Reviewed, UniProtKB/Swiss-Prot P24091 (CHI2_TOBAC)

Names and origin

Protein names

Recommended name:
    Endochitinase B
      Short name=CHN-B
    EC=3.2.1.14

Gene names

Name:

CHN50

Organism

Nicotiana tabacum (Common tobacco)

Taxonomic identifier

4097 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Nicotianoideae › Nicotianeae › Nicotiana

Protein attributes

Sequence length	324 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Defense against chitin containing fungal pathogens.
Catalytic activity	Random hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.
Subcellular location	Vacuole. Note: Vacuolar and protoplast. Ref.4
Induction	By ethylene.
Post-translational modification	The 4-hydroxyproline residues are not glycosylated in this plant vacuolar protein.
Sequence similarities	Belongs to the glycosyl hydrolase 19 family. Chitinase class I subfamily. Contains 1 chitin-binding type-1 domain.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Chitin degradation Hypersensitive response Plant defense Polysaccharide degradation
Cellular component	Vacuole
Domain	Signal
Ligand	Chitin-binding
Molecular function	Glycosidase Hydrolase Pathogenesis-related protein
PTM	Disulfide bond Hydroxylation
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	cell wall macromolecule catabolic process Inferred from electronic annotation. Source: InterPro chitin catabolic process Inferred from electronic annotation. Source: UniProtKB-KW plant-type hypersensitive response Inferred from electronic annotation. Source: UniProtKB-KW polysaccharide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW response to biotic stimulus Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	vacuole Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	chitin binding Inferred from electronic annotation. Source: UniProtKB-KW chitinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	Ref.3
	Chain	24 – 317	294	Endochitinase B		PRO_0000005332
	Propeptide	318 – 324	7	Removed in mature form Probable		PRO_0000005333
Regions
	Domain	24 – 65	42	Chitin-binding type-1
Amino acid modifications
	Modified residue	67	1	4-hydroxyproline Ref.5
	Modified residue	69	1	4-hydroxyproline Ref.5
	Disulfide bond	26 ↔ 41		By similarity
	Disulfide bond	35 ↔ 47		By similarity
	Disulfide bond	40 ↔ 54		By similarity
	Disulfide bond	59 ↔ 63		By similarity
	Disulfide bond	96 ↔ 158		By similarity
	Disulfide bond	170 ↔ 178		By similarity
	Disulfide bond	277 ↔ 309		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P24091-1 [UniParc]. Last modified March 1, 1992. Version 1. Checksum: FA65DC2113B33EB6	FASTA	324	34,721
	10 20 30 40 50 60 MRLREFTALS SLLFSLLLLS ASAEQCGSQA GGARCASGLC CSKFGWCGNT NDYCGPGNCQ 70 80 90 100 110 120 SQCPGGPTPP GGGDLGSIIS SSMFDQMLKH RNDNACQGKG FYSYNAFINA ARSFPGFGTS 130 140 150 160 170 180 GDTTARKREI AAFFAQTSHE TTGGWATAPD GPYAWGYCWL REQGSPGDYC TPSGQWPCAP 190 200 210 220 230 240 GRKYFGRGPI QISHNYNYGP CGRAIGVDLL NNPDLVATDP VISFKSALWF WMTPQSPKPS 250 260 270 280 290 300 CHDVIIGRWQ PSSADRAANR LPGFGVITNI INGGLECGRG TDSRVQDRIG FYRRYCSILG 310 320 VSPGDNLDCG NQRSFGNGLL VDTM

References

[1]	"Gene structure and expression of a tobacco endochitinase gene in suspension-cultured tobacco cells." Fukuda Y., Ohme M., Shinshi H. Plant Mol. Biol. 16:1-10(1991) [PubMed: 1888889] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. Bright Yellow 4. Tissue: Leaf.
[2]	"The structure and regulation of homeologous tobacco endochitinase genes of Nicotiana sylvestris and N. tomentosiformis origin." van Buuren M., Neuhaus J.-M., Shinshi H., Ryals J., Meins F. Jr. Mol. Gen. Genet. 232:460-469(1992) [PubMed: 1588915] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. Havana 425. Tissue: Leaf.
[3]	"Regulation of a plant pathogenesis-related enzyme: inhibition of chitinase and chitinase mRNA accumulation in cultured tobacco tissues by auxin and cytokinin." Shinshi H., Mohnen D., Meins F. Jr. Proc. Natl. Acad. Sci. U.S.A. 84:89-93(1987) [PubMed: 16593796] [Abstract] Cited for: NUCLEOTIDE SEQUENCE OF 15-324, PROTEIN SEQUENCE OF 24-53. Strain: cv. Havana.
[4]	"A short C-terminal sequence is necessary and sufficient for the targeting of chitinases to the plant vacuole." Neuhaus J.-M., Sticher L., Meins F. Jr., Boller T. Proc. Natl. Acad. Sci. U.S.A. 88:10362-10366(1991) [PubMed: 1946457] [Abstract] Cited for: SUBCELLULAR LOCATION.
[5]	"Vacuolar chitinases of tobacco: a new class of hydroxyproline-containing proteins." Sticher L., Hofsteenge J., Milani A., Neuhaus J.-M., Meins F. Jr. Science 257:655-657(1992) [PubMed: 1496378] [Abstract] Cited for: HYDROXYLATION AT PRO-67 AND PRO-69, MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X51599 Genomic DNA. Translation: CAA35945.1. X64519 Genomic DNA. Translation: CAA45822.1. M15173 mRNA. Translation: AAA34070.1.
PIR	S20981.
3D structure databases
HSSP	HSSP built from PDB template 1CNS based on UniProtKB P23951.
SMR	P24091. Positions 24-315.
ModBase	Search...
Protein family/group databases
CAZy	CBM18. Carbohydrate-Binding Module Family 18. GH19. Glycoside Hydrolase Family 19.
Enzyme and pathway databases
BRENDA	3.2.1.14. 298.
Family and domain databases
InterPro	IPR018371. Chitin-binding_1_CS. IPR001002. Chitin_bd_1. IPR016283. Glyco_hydro_19. IPR000726. Glyco_hydro_19_cat. [Graphical view]
Gene3D	G3DSA:3.30.60.10. Chitin_bd_1. 1 hit.
PANTHER	PTHR22595. Glyco_hydro_19_cat. 1 hit.
Pfam	PF00187. Chitin_bind_1. 1 hit. PF00182. Glyco_hydro_19. 1 hit. [Graphical view]
PIRSF	PIRSF001060. Endochitinase. 1 hit.
PRINTS	PR00451. CHITINBINDNG.
ProDom	PD000609. Chitin_binding_1. 1 hit. PD354900. Glyco_hydro_19. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00270. ChtBD1. 1 hit. [Graphical view]
PROSITE	PS00026. CHIT_BIND_I_1. 1 hit. PS50941. CHIT_BIND_I_2. 1 hit. PS00773. CHITINASE_19_1. 1 hit. PS00774. CHITINASE_19_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CHI2_TOBAC

Accession

Primary (citable) accession number: P24091

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 1992
Last sequence update:	March 1, 1992
Last modified:	June 16, 2009
This is version 88 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)