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Protein

Alpha-2-HS-glycoprotein

Gene

Ahsg

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Could inhibit both insulin-receptor tyrosine kinase activity and insulin-stimulated receptor autophosphorylation and, concomitantly, antagonize the mitogenic effect of the hormone in cultured rat hepatoma cells.

GO - Molecular functioni

GO - Biological processi

  • acute-phase response Source: UniProtKB
  • animal organ regeneration Source: RGD
  • cellular response to insulin stimulus Source: RGD
  • cerebral cortex development Source: RGD
  • male gonad development Source: RGD
  • negative regulation of bone mineralization Source: RGD
  • negative regulation of cell growth Source: RGD
  • negative regulation of insulin receptor signaling pathway Source: RGD
  • ossification Source: RGD
  • positive regulation of bone resorption Source: RGD
  • positive regulation of phagocytosis Source: UniProtKB
  • regulation of inflammatory response Source: UniProtKB

Protein family/group databases

MEROPSiI25.020

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-2-HS-glycoprotein
Alternative name(s):
59 kDa bone sialic acid-containing protein
Short name:
BSP
Fetuin-A
Glycoprotein PP63
Gene namesi
Name:Ahsg
Synonyms:Fetua
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi2075 Ahsg

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 181 PublicationAdd BLAST18
ChainiPRO_000000889719 – 352Alpha-2-HS-glycoproteinAdd BLAST334

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi32 ↔ 343PROSITE-ProRule annotation
Disulfide bondi89 ↔ 100PROSITE-ProRule annotation
Glycosylationi99N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi114 ↔ 132PROSITE-ProRule annotation
Modified residuei134PhosphoserineBy similarity1
Modified residuei135PhosphothreonineBy similarity1
Modified residuei138PhosphoserineCombined sources1
Disulfide bondi146 ↔ 149PROSITE-ProRule annotation
Glycosylationi156N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi176N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi208 ↔ 219PROSITE-ProRule annotation
Disulfide bondi230 ↔ 247PROSITE-ProRule annotation
Modified residuei309PhosphoserineCombined sources1
Modified residuei313PhosphoserineCombined sources1
Modified residuei316PhosphoserineCombined sources1
Modified residuei318PhosphoserineCombined sources1

Post-translational modificationi

Undergoes complex post-translational modification involving N-glycosylation, and addition of fucose and sialic acid residues. Phosphorylation occurs at a serine residue.
Phosphorylated by FAM20C in the extracellular medium.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei143 – 144Cleavage; by trypsinSequence analysis2

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PRIDEiP24090

PTM databases

iPTMnetiP24090
PhosphoSitePlusiP24090

Expressioni

Tissue specificityi

Synthesized in liver and secreted by the hepatocytes in the blood.

Interactioni

Protein-protein interaction databases

IntActiP24090, 2 interactors

Structurei

3D structure databases

ProteinModelPortaliP24090
SMRiP24090
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini19 – 133Cystatin fetuin-A-type 1PROSITE-ProRule annotationAdd BLAST115
Domaini144 – 250Cystatin fetuin-A-type 2PROSITE-ProRule annotationAdd BLAST107

Sequence similaritiesi

Belongs to the fetuin family.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

HOGENOMiHOG000290189
HOVERGENiHBG051607
InParanoidiP24090

Family and domain databases

CDDicd00042 CY, 2 hits
InterProiView protein in InterPro
IPR000010 Cystatin_dom
IPR025760 Cystatin_Fetuin_A
IPR001363 Prot_inh_fetuin_CS
PfamiView protein in Pfam
PF00031 Cystatin, 1 hit
SMARTiView protein in SMART
SM00043 CY, 2 hits
PROSITEiView protein in PROSITE
PS51529 CYSTATIN_FETUIN_A, 2 hits
PS01254 FETUIN_1, 1 hit
PS01255 FETUIN_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P24090-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKSLVLLLCF AQLWSCQSAP QGAGLGFREL ACDDPETEHV ALIAVDYLNK
60 70 80 90 100
HLLQGFRQIL NQIDKVKVWS RRPFGEVYEL EIDTLETTCH ALDPTPLANC
110 120 130 140 150
SVRQQAEHAV EGDCDFHILK QDGQFRVLHA QCHSTPDSAE DVRKFCPRCP
160 170 180 190 200
ILIRFNDTNV VHTVKTALAA FNAQNNGTYF KLVEISRAQN VPFPVSTLVE
210 220 230 240 250
FVIAATDCTG QEVTDPAKCN LLAEKQYGFC KATLIHRLGG EEVSVACKLF
260 270 280 290 300
QTQPQPANAN PAGPAPTVGQ AAPVAPPAGP PESVVVGPVA VPLGLPDHRT
310 320 330 340 350
HHDLRHAFSP VASVESASGE VLHSPKVGQP GDAGAAGPVA PLCPGRVRYF

KI
Length:352
Mass (Da):37,982
Last modified:April 1, 1993 - v2
Checksum:i43564F60F3C7C90A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti21Q → E AA sequence (PubMed:1860865).Curated1
Sequence conflicti29E → Q AA sequence (PubMed:1860865).Curated1
Sequence conflicti33 – 34DD → NN AA sequence (PubMed:1860865).Curated2
Sequence conflicti46D → H in AAA75502 (PubMed:1849862).Curated1
Sequence conflicti76E → Q in AAA75502 (PubMed:1849862).Curated1
Sequence conflicti236 – 237HR → QF AA sequence (PubMed:1860865).Curated2
Sequence conflicti241 – 242EE → QQ AA sequence (PubMed:1860865).Curated2
Sequence conflicti329Q → E AA sequence (PubMed:1860865).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X63446 mRNA Translation: CAA45042.1
M29758 mRNA Translation: AAA75502.1
D10261 mRNA Translation: BAA01101.1
BC091118 mRNA Translation: AAH91118.1
PIRiA32827
RefSeqiNP_037030.1, NM_012898.4
UniGeneiRn.32083

Genome annotation databases

GeneIDi25373
KEGGirno:25373
UCSCiRGD:2075 rat

Similar proteinsi

Entry informationi

Entry nameiFETUA_RAT
AccessioniPrimary (citable) accession number: P24090
Secondary accession number(s): Q5BKD2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: April 1, 1993
Last modified: April 25, 2018
This is version 136 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome
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Main funding by: National Institutes of Health