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Protein

Alpha-2-HS-glycoprotein

Gene

Ahsg

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Could inhibit both insulin-receptor tyrosine kinase activity and insulin-stimulated receptor autophosphorylation and, concomitantly, antagonize the mitogenic effect of the hormone in cultured rat hepatoma cells.

GO - Molecular functioni

GO - Biological processi

  • acute-phase response Source: UniProtKB
  • animal organ regeneration Source: RGD
  • cellular response to insulin stimulus Source: RGD
  • cerebral cortex development Source: RGD
  • male gonad development Source: RGD
  • negative regulation of bone mineralization Source: UniProtKB
  • negative regulation of cell growth Source: RGD
  • negative regulation of insulin receptor signaling pathway Source: RGD
  • positive regulation of bone resorption Source: RGD
  • positive regulation of phagocytosis Source: UniProtKB
  • protein complex assembly Source: RGD
  • regulation of inflammatory response Source: UniProtKB
Complete GO annotation...

Protein family/group databases

MEROPSiI25.020.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-2-HS-glycoprotein
Alternative name(s):
59 kDa bone sialic acid-containing protein
Short name:
BSP
Fetuin-A
Glycoprotein PP63
Gene namesi
Name:Ahsg
Synonyms:Fetua
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi2075. Ahsg.

Subcellular locationi

GO - Cellular componenti

  • blood microparticle Source: GO_Central
  • extracellular matrix Source: RGD
  • extracellular space Source: RGD
  • protein complex Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 181 PublicationAdd BLAST18
ChainiPRO_000000889719 – 352Alpha-2-HS-glycoproteinAdd BLAST334

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi32 ↔ 343PROSITE-ProRule annotation
Disulfide bondi89 ↔ 100PROSITE-ProRule annotation
Glycosylationi99N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi114 ↔ 132PROSITE-ProRule annotation
Modified residuei134PhosphoserineBy similarity1
Modified residuei135PhosphothreonineBy similarity1
Modified residuei138PhosphoserineCombined sources1
Disulfide bondi146 ↔ 149PROSITE-ProRule annotation
Glycosylationi156N-linked (GlcNAc...)Sequence analysis1
Glycosylationi176N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi208 ↔ 219PROSITE-ProRule annotation
Disulfide bondi230 ↔ 247PROSITE-ProRule annotation
Modified residuei309PhosphoserineCombined sources1
Modified residuei313PhosphoserineCombined sources1
Modified residuei316PhosphoserineCombined sources1
Modified residuei318PhosphoserineCombined sources1

Post-translational modificationi

Undergoes complex post-translational modification involving N-glycosylation, and addition of fucose and sialic acid residues. Phosphorylation occurs at a serine residue.
Phosphorylated by FAM20C in the extracellular medium.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei143 – 144Cleavage; by trypsinSequence analysis2

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PRIDEiP24090.

PTM databases

iPTMnetiP24090.
PhosphoSitePlusiP24090.

Expressioni

Tissue specificityi

Synthesized in liver and secreted by the hepatocytes in the blood.

Interactioni

Protein-protein interaction databases

IntActiP24090. 2 interactors.

Structurei

3D structure databases

ProteinModelPortaliP24090.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini19 – 133Cystatin fetuin-A-type 1PROSITE-ProRule annotationAdd BLAST115
Domaini144 – 250Cystatin fetuin-A-type 2PROSITE-ProRule annotationAdd BLAST107

Sequence similaritiesi

Belongs to the fetuin family.PROSITE-ProRule annotation
Contains 2 cystatin fetuin-A-type domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

HOGENOMiHOG000290189.
HOVERGENiHBG051607.
InParanoidiP24090.

Family and domain databases

CDDicd00042. CY. 2 hits.
InterProiIPR000010. Cystatin_dom.
IPR025760. Cystatin_Fetuin_A.
IPR001363. Prot_inh_fetuin_CS.
[Graphical view]
PfamiPF00031. Cystatin. 1 hit.
[Graphical view]
SMARTiSM00043. CY. 2 hits.
[Graphical view]
PROSITEiPS51529. CYSTATIN_FETUIN_A. 2 hits.
PS01254. FETUIN_1. 1 hit.
PS01255. FETUIN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P24090-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKSLVLLLCF AQLWSCQSAP QGAGLGFREL ACDDPETEHV ALIAVDYLNK
60 70 80 90 100
HLLQGFRQIL NQIDKVKVWS RRPFGEVYEL EIDTLETTCH ALDPTPLANC
110 120 130 140 150
SVRQQAEHAV EGDCDFHILK QDGQFRVLHA QCHSTPDSAE DVRKFCPRCP
160 170 180 190 200
ILIRFNDTNV VHTVKTALAA FNAQNNGTYF KLVEISRAQN VPFPVSTLVE
210 220 230 240 250
FVIAATDCTG QEVTDPAKCN LLAEKQYGFC KATLIHRLGG EEVSVACKLF
260 270 280 290 300
QTQPQPANAN PAGPAPTVGQ AAPVAPPAGP PESVVVGPVA VPLGLPDHRT
310 320 330 340 350
HHDLRHAFSP VASVESASGE VLHSPKVGQP GDAGAAGPVA PLCPGRVRYF

KI
Length:352
Mass (Da):37,982
Last modified:April 1, 1993 - v2
Checksum:i43564F60F3C7C90A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti21Q → E AA sequence (PubMed:1860865).Curated1
Sequence conflicti29E → Q AA sequence (PubMed:1860865).Curated1
Sequence conflicti33 – 34DD → NN AA sequence (PubMed:1860865).Curated2
Sequence conflicti46D → H in AAA75502 (PubMed:1849862).Curated1
Sequence conflicti76E → Q in AAA75502 (PubMed:1849862).Curated1
Sequence conflicti236 – 237HR → QF AA sequence (PubMed:1860865).Curated2
Sequence conflicti241 – 242EE → QQ AA sequence (PubMed:1860865).Curated2
Sequence conflicti329Q → E AA sequence (PubMed:1860865).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X63446 mRNA. Translation: CAA45042.1.
M29758 mRNA. Translation: AAA75502.1.
D10261 mRNA. Translation: BAA01101.1.
BC091118 mRNA. Translation: AAH91118.1.
PIRiA32827.
RefSeqiNP_037030.1. NM_012898.4.
UniGeneiRn.32083.

Genome annotation databases

GeneIDi25373.
KEGGirno:25373.
UCSCiRGD:2075. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X63446 mRNA. Translation: CAA45042.1.
M29758 mRNA. Translation: AAA75502.1.
D10261 mRNA. Translation: BAA01101.1.
BC091118 mRNA. Translation: AAH91118.1.
PIRiA32827.
RefSeqiNP_037030.1. NM_012898.4.
UniGeneiRn.32083.

3D structure databases

ProteinModelPortaliP24090.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP24090. 2 interactors.

Protein family/group databases

MEROPSiI25.020.

PTM databases

iPTMnetiP24090.
PhosphoSitePlusiP24090.

Proteomic databases

PRIDEiP24090.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi25373.
KEGGirno:25373.
UCSCiRGD:2075. rat.

Organism-specific databases

CTDi197.
RGDi2075. Ahsg.

Phylogenomic databases

HOGENOMiHOG000290189.
HOVERGENiHBG051607.
InParanoidiP24090.

Miscellaneous databases

PROiP24090.

Family and domain databases

CDDicd00042. CY. 2 hits.
InterProiIPR000010. Cystatin_dom.
IPR025760. Cystatin_Fetuin_A.
IPR001363. Prot_inh_fetuin_CS.
[Graphical view]
PfamiPF00031. Cystatin. 1 hit.
[Graphical view]
SMARTiSM00043. CY. 2 hits.
[Graphical view]
PROSITEiPS51529. CYSTATIN_FETUIN_A. 2 hits.
PS01254. FETUIN_1. 1 hit.
PS01255. FETUIN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiFETUA_RAT
AccessioniPrimary (citable) accession number: P24090
Secondary accession number(s): Q5BKD2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: April 1, 1993
Last modified: November 30, 2016
This is version 130 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.