P24069 (HAD1_PSEUC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: (S)-2-haloacid dehalogenase 1 EC=3.8.1.2 Alternative name(s): 2-haloalkanoic acid dehalogenase I DEHCI Halocarboxylic acid halidohydrolase I L-2-haloacid dehalogenase I
Organism	Pseudomonas sp. (strain CBS-3)
Taxonomic identifier	72586 [NCBI]
Taxonomic lineage	Bacteria › Proteobacteria

Protein attributes

Sequence length	227 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the hydrolytic dehalogenation of small (S)-2-haloalkanoic acids to yield the corresponding (R)-2-hydroxyalkanoic acids. Acts on acids of short chain lengths, C2 to C4, with inversion of configuration at C-2.
Catalytic activity	(S)-2-haloacid + H2O = (R)-2-hydroxyacid + halide.
Sequence similarities	Belongs to the HAD-like hydrolase superfamily. S-2-haloalkanoic acid dehalogenase family.

Ontologies

Keywords
Molecular function	Hydrolase
Gene Ontology (GO)
Molecular function	(S)-2-haloacid dehalogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 227	227	(S)-2-haloacid dehalogenase 1		PRO_0000079160
Sites
	Active site	10	1	Nucleophile By similarity

Sequences

	Sequence		Length	Mass (Da)
	P24069 [UniParc]. Last modified October 1, 1993. Version 2. Checksum: 0D2002A0282C92EE	FASTA	227	25,430
	10 20 30 40 50 60 MDPIRACVFD AYGTLLDVNT AVMKHAHDIG GCAEELSSLW RQRQLEYSWT RTLMGRYADF 70 80 90 100 110 120 WQLTTEALDF ALESFGLLER TDLKNRLLDA YHELSAYPDA VGTLGALKAA GFTTAILSNG 130 140 150 160 170 180 NNEMLRGALR AGNLTEALDQ CISVDEIKIY KPDPRVYQFA CDRLDVRPSE VCFVSSNAWD 190 200 210 220 IGGAGAFGFN TVRINRINKP QEYSFAPQRH QLSSLSELPQ LLLRLTQ

References

[1]

"Complete nucleotide sequences and comparison of the structural genes of two 2-haloalkanoic acid dehalogenases from Pseudomonas sp. strain CBS3." Schneider B., Mueller R., Frank R., Lingens F. J. Bacteriol. 173:1530-1535(1991) [PubMed: 1995594] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	M62908 Genomic DNA. Translation: AAA63640.1.
PIR	A38452.
3D structure databases
ProteinModelPortal	P24069.
SMR	P24069. Positions 1-224.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR005834. Dehalogen-like_hydro. IPR023214. HAD-like_dom. IPR006388. HAD-SF_hydro_IA_v2. IPR006402. HAD-SF_hydro_IA_v3. IPR006328. HAD_II. IPR005833. Haloacid_DH/epoxide_hydro. [Graphical view]
Gene3D	G3DSA:3.40.50.1000. HAD-like_dom. 1 hit.
Pfam	PF00702. Hydrolase. 1 hit. [Graphical view]
PRINTS	PR00413. HADHALOGNASE.
SUPFAM	SSF56784. HAD-like_dom. 1 hit.
TIGRFAMs	TIGR01493. HAD-SF-IA-v2. 1 hit. TIGR01509. HAD-SF-IA-v3. 1 hit. TIGR01428. HAD_type_II. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HAD1_PSEUC

Accession

Primary (citable) accession number: P24069

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 1992
Last sequence update:	October 1, 1993
Last modified:	May 31, 2011
This is version 52 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program