P24067 (BIP2_MAIZE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Luminal-binding protein 2
Short name=BiP2

Alternative name(s):
B-70
Short name=B70
Heat shock protein 70 homolog 2

Gene names

Name:

BIPE2

Organism

Zea mays (Maize)

Taxonomic identifier

4577 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › Liliopsida › commelinids › Poales › Poaceae › PACMAD clade › Panicoideae › Andropogoneae › Zea

Protein attributes

Sequence length	663 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Probably plays a role in facilitating the assembly of multimeric protein complexes inside the ER.
Subcellular location	Endoplasmic reticulum lumen.
Induction	By treatment with tunicamycin. Overproduced in fl2, defective endosperm-b30, and mucronate mutants.
Sequence similarities	Belongs to the heat shock protein 70 family.
Sequence caution	The sequence AAA92743.1 differs from that shown. Reason: Erroneous termination at position 664. Translated as stop.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum
Domain	Signal
Ligand	ATP-binding Nucleotide-binding
PTM	Glycoprotein
Gene Ontology (GO)
Biological_process	polar nucleus fusion Inferred from electronic annotation. Source: EnsemblPlants/Gramene response to cadmium ion Inferred from electronic annotation. Source: EnsemblPlants/Gramene response to endoplasmic reticulum stress Inferred from electronic annotation. Source: EnsemblPlants/Gramene response to heat Inferred from electronic annotation. Source: EnsemblPlants/Gramene
Cellular_component	cell wall Inferred from electronic annotation. Source: EnsemblPlants/Gramene endoplasmic reticulum lumen Inferred from electronic annotation. Source: UniProtKB-SubCell nucleolus Inferred from electronic annotation. Source: EnsemblPlants/Gramene plasma membrane Inferred from electronic annotation. Source: EnsemblPlants/Gramene vacuole Inferred from electronic annotation. Source: EnsemblPlants/Gramene
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 21	21	Potential
	Chain	22 – 663	642	Luminal-binding protein 2		PRO_0000013591
Regions
	Motif	660 – 663	4	Prevents secretion from ER
Amino acid modifications
	Glycosylation	614	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	P24067 [UniParc]. Last modified December 15, 1998. Version 3. Checksum: 6769B7D19A0D918A	FASTA	663	73,085
	10 20 30 40 50 60 MDRARGSAFL LGVLLAGSLF AFSVAKEETK KLGTVIGIDL GTTYSCVGVY KNGHVEIIAN 70 80 90 100 110 120 DQGNRITPSW VAFTDSERLI GEAAKNQAAV NPERTIFDVK RLIGRKFADK EVQRDMKLVP 130 140 150 160 170 180 YKIINKDGKP YIQVKIKDGE NKVFSPEEIS AMILGKMKDT AEAYLGKKIN DAVVTVPAYF 190 200 210 220 230 240 NDAQRQATKD AGVIAGLNVA RIINEPTAAA IAYGLDKKGG EKNILVFDLG GGTFDVSILT 250 260 270 280 290 300 IDNGVFEVLA TNGDTHLGGE DFDQRIMEYF IKLIKKKYSK DISKDNRALG KLRREAERAK 310 320 330 340 350 360 RALSNQHQVR VEIESLFDGT DFSEPLTRAR FEELNNDLFR KTMGPVKKAM EDAGLEKSQI 370 380 390 400 410 420 HEIVLVGGST RIPKVQQLLR DYFDGKEPNK GVNPDEAVAF GAAVQGSILS GEGGDETKDI 430 440 450 460 470 480 LLLDVAPLTL GIETVGGVMT KLIPRNTVIP TKKSQVFTTY QDQQTTVSIQ VFEGERSMTK 490 500 510 520 530 540 DCRLLGKFDL NGIAPAPRGT PQIEVTFEVD ANGILNVKAE DKGTGKSEKI TITNEKGRLS 550 560 570 580 590 600 QEEIDRMVRE AEEFAEEDKK VKERIDARNQ LETYVYNMKN TVGDKDKLAD KLEAEEKEKV 610 620 630 640 650 660 EEALKEALEW LDDNQSAEKE DYEEKLKEVE AVCNPIVSAV YQRSGGAPGG DADGGVDDDH DEL

References

[1]	"Comparative analysis of BiP gene expression in maize endosperm." Wrobel R.L., Obrian G.R., Boston R.S. Gene 204:105-113(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION. Strain: cv. Floury-2. Tissue: Kernel.
[2]	"Characterization of an immunoglobulin binding protein homolog in the maize floury-2 endosperm mutant." Fontes E.B.P., Shank B.B., Wrobel R.L., Moose S.P., Obrian G.R., Wurtzel E.T., Boston R.S. Plant Cell 3:483-496(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 197-663, CHARACTERIZATION. Strain: cv. Floury-2. Tissue: Kernel.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U58208 mRNA. Translation: AAC49899.1. M59449 mRNA. Translation: AAA92743.1. Sequence problems.
PIR	JQ0966. T04078.
RefSeq	NP_001105893.1. NM_001112423.1.
UniGene	Zm.80917.
3D structure databases
ProteinModelPortal	P24067.
ModBase	Search...
Proteomic databases
PRIDE	P24067.
ProMEX	P24067.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	732808.
KEGG	zma:732808.
Organism-specific databases
Gramene	P24067.
MaizeGDB	66083.
Phylogenomic databases
HOGENOM	HOG000228135.
KO	K09490.
OMA	DKRAVQK.
Family and domain databases
InterPro	IPR018181. Heat_shock_70_CS. IPR013126. Hsp_70_fam. [Graphical view]
Pfam	PF00012. HSP70. 1 hit. [Graphical view]
PRINTS	PR00301. HEATSHOCK70.
PROSITE	PS00014. ER_TARGET. 1 hit. PS00297. HSP70_1. 1 hit. PS00329. HSP70_2. 1 hit. PS01036. HSP70_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BIP2_MAIZE

Accession

Primary (citable) accession number: P24067

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 1992
Last sequence update:	December 15, 1998
Last modified:	April 3, 2013
This is version 84 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program