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Protein

Integrin alpha-L

Gene

Itgal

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4 (PubMed:2051027). Integrin alpha-L/beta-2 is also a receptor for F11R (By similarity). Involved in a variety of immune phenomena including leukocyte-endothelial cell interaction, cytotoxic T-cell mediated killing, and antibody dependent killing by granulocytes and monocytes. Contributes to natural killer cell cytotoxicity. Involved in leukocyte adhesion and transmigration of leukocytes including T-cells and neutrophils (PubMed:16234355, PubMed:24158516). Required for generation of common lymphoid progenitor cells in bone marrow, indicating the role in lymphopoiesis (PubMed:25108025). Integrin alpha-L/beta-2 in association with ICAM3, contributes to apoptotic neutrophil phagocytosis by macrophages.By similarity4 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi466 – 474Sequence analysis9
Calcium bindingi528 – 536Sequence analysis9
Calcium bindingi588 – 596Sequence analysis9

GO - Molecular functioni

GO - Biological processi

  • activated T cell proliferation Source: MGI
  • cell-matrix adhesion Source: MGI
  • cell surface receptor signaling pathway Source: MGI
  • heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules Source: MGI
  • integrin-mediated signaling pathway Source: UniProtKB-KW
  • leukocyte cell-cell adhesion Source: MGI
  • phagocytosis Source: UniProtKB-KW
  • positive regulation of calcium-mediated signaling Source: MGI
  • positive regulation of cell-cell adhesion Source: MGI
  • positive regulation of T cell proliferation Source: MGI
  • receptor clustering Source: MGI
  • regulation of cell-cell adhesion Source: MGI
  • T cell activation via T cell receptor contact with antigen bound to MHC molecule on antigen presenting cell Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Integrin, Receptor

Keywords - Biological processi

Cell adhesion, Phagocytosis

Keywords - Ligandi

Calcium, Magnesium, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-198933. Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell.
R-MMU-202733. Cell surface interactions at the vascular wall.
R-MMU-216083. Integrin cell surface interactions.
R-MMU-6798695. Neutrophil degranulation.

Names & Taxonomyi

Protein namesi
Recommended name:
Integrin alpha-L
Alternative name(s):
CD11 antigen-like family member A
Leukocyte adhesion glycoprotein LFA-1 alpha chain
Short name:
LFA-1A
Leukocyte function-associated molecule 1 alpha chain
Lymphocyte antigen 15
Short name:
Ly-15
CD_antigen: CD11a
Gene namesi
Name:Itgal
Synonyms:Lfa-1, Ly-15
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:96606. Itgal.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini24 – 1087ExtracellularSequence analysisAdd BLAST1064
Transmembranei1088 – 1108HelicalSequence analysisAdd BLAST21
Topological domaini1109 – 1163CytoplasmicSequence analysisAdd BLAST55

GO - Cellular componenti

  • cell-cell junction Source: MGI
  • cell surface Source: MGI
  • external side of plasma membrane Source: MGI
  • extracellular exosome Source: MGI
  • immunological synapse Source: MGI
  • integrin alphaL-beta2 complex Source: MGI
  • membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Mice show decreased cellularity in thymus but not spleen, and impaired early T cell development (PubMed:25108025). Obese mutant mice show decreased total number of T-cells, lower levels of neutrophil elastase and reduced cytotoxic T-cell proliferation in adipose tissue, as well as improved glucose tolerance and insulin resistance in comparison to obese wild type mice (PubMed:24158516).2 Publications

Chemistry databases

ChEMBLiCHEMBL1075188.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 231 PublicationAdd BLAST23
ChainiPRO_000001629324 – 1163Integrin alpha-LAdd BLAST1140

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi70 ↔ 77By similarity
Glycosylationi86N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi108 ↔ 126By similarity
Disulfide bondi147 ↔ 199By similarity
Glycosylationi185N-linked (GlcNAc...)Sequence analysis1
Glycosylationi270N-linked (GlcNAc...)Sequence analysis1
Glycosylationi444N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi651 ↔ 705By similarity
Glycosylationi668N-linked (GlcNAc...)Sequence analysis1
Glycosylationi696N-linked (GlcNAc...)Sequence analysis1
Glycosylationi724N-linked (GlcNAc...)Sequence analysis1
Glycosylationi728N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi767 ↔ 773By similarity
Glycosylationi776N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi840 ↔ 856By similarity
Glycosylationi857N-linked (GlcNAc...)Sequence analysis1
Glycosylationi880N-linked (GlcNAc...)Sequence analysis1
Glycosylationi890N-linked (GlcNAc...)Sequence analysis1
Glycosylationi899N-linked (GlcNAc...)Sequence analysis1
Glycosylationi927N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi993 ↔ 1009By similarity
Disulfide bondi1017 ↔ 1048By similarity
Glycosylationi1056N-linked (GlcNAc...)Sequence analysis1

Post-translational modificationi

In resting T-cells, up to 40% of surface ITGAL is constitutively phosphorylated. Phosphorylation causes conformational changes needed for ligand binding and is necessary for the activation by some physiological agents.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

EPDiP24063.
MaxQBiP24063.
PaxDbiP24063.
PRIDEiP24063.

PTM databases

iPTMnetiP24063.
PhosphoSitePlusiP24063.
SwissPalmiP24063.

Expressioni

Tissue specificityi

Leukocytes.2 Publications

Gene expression databases

BgeeiENSMUSG00000030830.
CleanExiMM_ITGAL.
ExpressionAtlasiP24063. baseline and differential.
GenevisibleiP24063. MM.

Interactioni

Subunit structurei

Heterodimer of an alpha and a beta subunit. Alpha-L associates with beta-2. Interacts with THBD.By similarity

GO - Molecular functioni

Protein-protein interaction databases

DIPiDIP-35643N.
IntActiP24063. 1 interactor.
STRINGi10090.ENSMUSP00000101913.

Chemistry databases

BindingDBiP24063.

Structurei

3D structure databases

ProteinModelPortaliP24063.
SMRiP24063.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati28 – 79FG-GAP 1PROSITE-ProRule annotationAdd BLAST52
Repeati80 – 138FG-GAP 2PROSITE-ProRule annotationAdd BLAST59
Domaini153 – 325VWFAPROSITE-ProRule annotationAdd BLAST173
Repeati336 – 387FG-GAP 3PROSITE-ProRule annotationAdd BLAST52
Repeati390 – 443FG-GAP 4PROSITE-ProRule annotationAdd BLAST54
Repeati444 – 504FG-GAP 5PROSITE-ProRule annotationAdd BLAST61
Repeati505 – 561FG-GAP 6PROSITE-ProRule annotationAdd BLAST57
Repeati565 – 625FG-GAP 7PROSITE-ProRule annotationAdd BLAST61

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi1111 – 1115GFFKR motif5

Domaini

The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage. The I-domain is necessary and sufficient for interaction with ICAM1 and F11R.By similarity

Sequence similaritiesi

Belongs to the integrin alpha chain family.Curated
Contains 7 FG-GAP repeats.PROSITE-ProRule annotation
Contains 1 VWFA domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IPAP. Eukaryota.
ENOG410ZJIF. LUCA.
GeneTreeiENSGT00760000118782.
HOGENOMiHOG000113114.
HOVERGENiHBG006188.
InParanoidiP24063.

Family and domain databases

InterProiIPR013517. FG-GAP.
IPR013519. Int_alpha_beta-p.
IPR000413. Integrin_alpha.
IPR013649. Integrin_alpha-2.
IPR018184. Integrin_alpha_C_CS.
IPR032695. Integrin_dom.
IPR002035. VWF_A.
[Graphical view]
PfamiPF01839. FG-GAP. 1 hit.
PF00357. Integrin_alpha. 1 hit.
PF08441. Integrin_alpha2. 1 hit.
PF00092. VWA. 1 hit.
[Graphical view]
PRINTSiPR01185. INTEGRINA.
SMARTiSM00191. Int_alpha. 5 hits.
SM00327. VWA. 1 hit.
[Graphical view]
SUPFAMiSSF53300. SSF53300. 1 hit.
SSF69179. SSF69179. 2 hits.
PROSITEiPS51470. FG_GAP. 7 hits.
PS00242. INTEGRIN_ALPHA. 1 hit.
PS50234. VWFA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P24063-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSFRIAGPRL LLLGLQLFAK AWSYNLDTRP TQSFLAQAGR HFGYQVLQIE
60 70 80 90 100
DGVVVGAPGE GDNTGGLYHC RTSSEFCQPV SLHGSNHTSK YLGMTLATDA
110 120 130 140 150
AKGSLLACDP GLSRTCDQNT YLSGLCYLFP QSLEGPMLQN RPAYQECMKG
160 170 180 190 200
KVDLVFLFDG SQSLDRKDFE KILEFMKDVM RKLSNTSYQF AAVQFSTDCR
210 220 230 240 250
TEFTFLDYVK QNKNPDVLLG SVQPMFLLTN TFRAINYVVA HVFKEESGAR
260 270 280 290 300
PDATKVLVII TDGEASDKGN ISAAHDITRY IIGIGKHFVS VQKQKTLHIF
310 320 330 340 350
ASEPVEEFVK ILDTFEKLKD LFTDLQRRIY AIEGTNRQDL TSFNMELSSS
360 370 380 390 400
GISADLSKGH AVVGAVGAKD WAGGFLDLRE DLQGATFVGQ EPLTSDVRGG
410 420 430 440 450
YLGYTVAWMT SRSSRPLLAA GAPRYQHVGQ VLLFQAPEAG GRWNQTQKIE
460 470 480 490 500
GTQIGSYFGG ELCSVDLDQD GEAELLLIGA PLFFGEQRGG RVFTYQRRQS
510 520 530 540 550
LFEMVSELQG DPGYPLGRFG AAITALTDIN GDRLTDVAVG APLEEQGAVY
560 570 580 590 600
IFNGKPGGLS PQPSQRIQGA QVFPGIRWFG RSIHGVKDLG GDRLADVVVG
610 620 630 640 650
AEGRVVVLSS RPVVDVVTEL SFSPEEIPVH EVECSYSARE EQKHGVKLKA
660 670 680 690 700
CFRIKPLTPQ FQGRLLANLS YTLQLDGHRM RSRGLFPDGS HELSGNTSIT
710 720 730 740 750
PDKSCLDFHF HFPICIQDLI SPINVSLNFS LLEEEGTPRD QKGRAMQPIL
760 770 780 790 800
RPSIHTVTKE IPFEKNCGED KKCEANLTLS SPARSGPLRL MSSASLAVEW
810 820 830 840 850
TLSNSGEDAY WVRLDLDFPR GLSFRKVEML QPHSRMPVSC EELTEGSSLL
860 870 880 890 900
TKTLKCNVSS PIFKAGQEVS LQVMFNTLLN SSWEDFVELN GTVHCENENS
910 920 930 940 950
SLQEDNSAAT HIPVLYPVNI LTKEQENSTL YISFTPKGPK TQQVQHVYQV
960 970 980 990 1000
RIQPSAYDHN MPTLEALVGV PWPHSEDPIT YTWSVQTDPL VTCHSEDLKR
1010 1020 1030 1040 1050
PSSEAEQPCL PGVQFRCPIV FRREILIQVT GTVELSKEIK ASSTLSLCSS
1060 1070 1080 1090 1100
LSVSFNSSKH FHLYGSKASE AQVLVKVDLI HEKEMLHVYV LSGIGGLVLL
1110 1120 1130 1140 1150
FLIFLALYKV GFFKRNLKEK MEADGGVPNG SPPEDTDPLA VPGEETKDMG
1160
CLEPSGRVTR TKA
Length:1,163
Mass (Da):128,328
Last modified:July 27, 2011 - v2
Checksum:iF19C786C4D33CC0C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti972W → R in AAA39426 (PubMed:2051027).Curated1
Sequence conflicti978P → L in AAA39426 (PubMed:2051027).Curated1
Sequence conflicti1023R → W in AAA39426 (PubMed:2051027).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M60778 mRNA. Translation: AAA39426.1.
AC133494 Genomic DNA. No translation available.
PIRiI56126.
UniGeneiMm.1618.

Genome annotation databases

EnsembliENSMUST00000170971; ENSMUSP00000131847; ENSMUSG00000030830.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M60778 mRNA. Translation: AAA39426.1.
AC133494 Genomic DNA. No translation available.
PIRiI56126.
UniGeneiMm.1618.

3D structure databases

ProteinModelPortaliP24063.
SMRiP24063.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-35643N.
IntActiP24063. 1 interactor.
STRINGi10090.ENSMUSP00000101913.

Chemistry databases

BindingDBiP24063.
ChEMBLiCHEMBL1075188.

PTM databases

iPTMnetiP24063.
PhosphoSitePlusiP24063.
SwissPalmiP24063.

Proteomic databases

EPDiP24063.
MaxQBiP24063.
PaxDbiP24063.
PRIDEiP24063.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000170971; ENSMUSP00000131847; ENSMUSG00000030830.

Organism-specific databases

MGIiMGI:96606. Itgal.

Phylogenomic databases

eggNOGiENOG410IPAP. Eukaryota.
ENOG410ZJIF. LUCA.
GeneTreeiENSGT00760000118782.
HOGENOMiHOG000113114.
HOVERGENiHBG006188.
InParanoidiP24063.

Enzyme and pathway databases

ReactomeiR-MMU-198933. Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell.
R-MMU-202733. Cell surface interactions at the vascular wall.
R-MMU-216083. Integrin cell surface interactions.
R-MMU-6798695. Neutrophil degranulation.

Miscellaneous databases

PROiP24063.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000030830.
CleanExiMM_ITGAL.
ExpressionAtlasiP24063. baseline and differential.
GenevisibleiP24063. MM.

Family and domain databases

InterProiIPR013517. FG-GAP.
IPR013519. Int_alpha_beta-p.
IPR000413. Integrin_alpha.
IPR013649. Integrin_alpha-2.
IPR018184. Integrin_alpha_C_CS.
IPR032695. Integrin_dom.
IPR002035. VWF_A.
[Graphical view]
PfamiPF01839. FG-GAP. 1 hit.
PF00357. Integrin_alpha. 1 hit.
PF08441. Integrin_alpha2. 1 hit.
PF00092. VWA. 1 hit.
[Graphical view]
PRINTSiPR01185. INTEGRINA.
SMARTiSM00191. Int_alpha. 5 hits.
SM00327. VWA. 1 hit.
[Graphical view]
SUPFAMiSSF53300. SSF53300. 1 hit.
SSF69179. SSF69179. 2 hits.
PROSITEiPS51470. FG_GAP. 7 hits.
PS00242. INTEGRIN_ALPHA. 1 hit.
PS50234. VWFA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiITAL_MOUSE
AccessioniPrimary (citable) accession number: P24063
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: July 27, 2011
Last modified: November 30, 2016
This is version 147 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.