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P24063

- ITAL_MOUSE

UniProt

P24063 - ITAL_MOUSE

Protein

Integrin alpha-L

Gene

Itgal

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 130 (01 Oct 2014)
      Sequence version 2 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. Is involved in a variety of immune phenomena including leukocyte-endothelial cell interaction, cytotoxic T-cell mediated killing, and antibody dependent killing by granulocytes and monocytes. Mice expressing a null mutation of the alpha-L subunit gene demonstrate impaired tumor rejection and impaired leukocytes recruitment.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Calcium bindingi466 – 4749Sequence Analysis
    Calcium bindingi528 – 5369Sequence Analysis
    Calcium bindingi588 – 5969Sequence Analysis

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. activated T cell proliferation Source: MGI
    2. cell surface receptor signaling pathway Source: MGI
    3. integrin-mediated signaling pathway Source: UniProtKB-KW
    4. leukocyte cell-cell adhesion Source: MGI
    5. positive regulation of calcium-mediated signaling Source: MGI
    6. positive regulation of cell-cell adhesion Source: MGI
    7. positive regulation of T cell proliferation Source: MGI
    8. regulation of cell-cell adhesion Source: MGI

    Keywords - Molecular functioni

    Integrin, Receptor

    Keywords - Biological processi

    Cell adhesion

    Keywords - Ligandi

    Calcium, Magnesium, Metal-binding

    Enzyme and pathway databases

    ReactomeiREACT_202937. Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell.
    REACT_216309. Integrin cell surface interactions.
    REACT_225233. Cell surface interactions at the vascular wall.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Integrin alpha-L
    Alternative name(s):
    CD11 antigen-like family member A
    Leukocyte adhesion glycoprotein LFA-1 alpha chain
    Short name:
    LFA-1A
    Leukocyte function-associated molecule 1 alpha chain
    Lymphocyte antigen 15
    Short name:
    Ly-15
    CD_antigen: CD11a
    Gene namesi
    Name:Itgal
    Synonyms:Lfa-1, Ly-15
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 7

    Organism-specific databases

    MGIiMGI:96606. Itgal.

    Subcellular locationi

    GO - Cellular componenti

    1. cell-cell junction Source: MGI
    2. external side of plasma membrane Source: MGI
    3. immunological synapse Source: MGI
    4. integrin complex Source: InterPro

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 23231 PublicationAdd
    BLAST
    Chaini24 – 11631140Integrin alpha-LPRO_0000016293Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi70 ↔ 77By similarity
    Glycosylationi86 – 861N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi108 ↔ 126By similarity
    Disulfide bondi147 ↔ 199By similarity
    Glycosylationi185 – 1851N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi270 – 2701N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi444 – 4441N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi651 ↔ 705By similarity
    Glycosylationi668 – 6681N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi696 – 6961N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi724 – 7241N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi728 – 7281N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi767 ↔ 773By similarity
    Glycosylationi776 – 7761N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi840 ↔ 856By similarity
    Glycosylationi857 – 8571N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi880 – 8801N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi890 – 8901N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi899 – 8991N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi927 – 9271N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi993 ↔ 1009By similarity
    Disulfide bondi1017 ↔ 1048By similarity
    Glycosylationi1056 – 10561N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    MaxQBiP24063.
    PaxDbiP24063.
    PRIDEiP24063.

    PTM databases

    PhosphoSiteiP24063.

    Expressioni

    Tissue specificityi

    Leukocytes.

    Gene expression databases

    ArrayExpressiP24063.
    BgeeiP24063.
    CleanExiMM_ITGAL.
    GenevestigatoriP24063.

    Interactioni

    Subunit structurei

    Heterodimer of an alpha and a beta subunit. Alpha-L associates with beta-2.

    Protein-protein interaction databases

    DIPiDIP-35643N.
    IntActiP24063. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliP24063.
    SMRiP24063. Positions 24-1068, 1078-1154.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini24 – 10871064ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini1109 – 116355CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei1088 – 110821HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati28 – 7952FG-GAP 1Add
    BLAST
    Repeati80 – 13859FG-GAP 2Add
    BLAST
    Domaini153 – 325173VWFAPROSITE-ProRule annotationAdd
    BLAST
    Repeati336 – 38752FG-GAP 3Add
    BLAST
    Repeati390 – 44354FG-GAP 4Add
    BLAST
    Repeati444 – 50461FG-GAP 5Add
    BLAST
    Repeati505 – 56157FG-GAP 6Add
    BLAST
    Repeati565 – 62561FG-GAP 7Add
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi1111 – 11155GFFKR motif

    Domaini

    The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage.

    Sequence similaritiesi

    Belongs to the integrin alpha chain family.Curated
    Contains 7 FG-GAP repeats.Curated
    Contains 1 VWFA domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat, Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG296688.
    GeneTreeiENSGT00750000117471.
    HOGENOMiHOG000113114.
    HOVERGENiHBG006188.

    Family and domain databases

    InterProiIPR013517. FG-GAP.
    IPR013519. Int_alpha_beta-p.
    IPR000413. Integrin_alpha.
    IPR013649. Integrin_alpha-2.
    IPR018184. Integrin_alpha_C_CS.
    IPR002035. VWF_A.
    [Graphical view]
    PfamiPF01839. FG-GAP. 1 hit.
    PF00357. Integrin_alpha. 1 hit.
    PF08441. Integrin_alpha2. 1 hit.
    PF00092. VWA. 1 hit.
    [Graphical view]
    PRINTSiPR01185. INTEGRINA.
    SMARTiSM00191. Int_alpha. 5 hits.
    SM00327. VWA. 1 hit.
    [Graphical view]
    SUPFAMiSSF53300. SSF53300. 1 hit.
    PROSITEiPS51470. FG_GAP. 7 hits.
    PS00242. INTEGRIN_ALPHA. 1 hit.
    PS50234. VWFA. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P24063-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSFRIAGPRL LLLGLQLFAK AWSYNLDTRP TQSFLAQAGR HFGYQVLQIE     50
    DGVVVGAPGE GDNTGGLYHC RTSSEFCQPV SLHGSNHTSK YLGMTLATDA 100
    AKGSLLACDP GLSRTCDQNT YLSGLCYLFP QSLEGPMLQN RPAYQECMKG 150
    KVDLVFLFDG SQSLDRKDFE KILEFMKDVM RKLSNTSYQF AAVQFSTDCR 200
    TEFTFLDYVK QNKNPDVLLG SVQPMFLLTN TFRAINYVVA HVFKEESGAR 250
    PDATKVLVII TDGEASDKGN ISAAHDITRY IIGIGKHFVS VQKQKTLHIF 300
    ASEPVEEFVK ILDTFEKLKD LFTDLQRRIY AIEGTNRQDL TSFNMELSSS 350
    GISADLSKGH AVVGAVGAKD WAGGFLDLRE DLQGATFVGQ EPLTSDVRGG 400
    YLGYTVAWMT SRSSRPLLAA GAPRYQHVGQ VLLFQAPEAG GRWNQTQKIE 450
    GTQIGSYFGG ELCSVDLDQD GEAELLLIGA PLFFGEQRGG RVFTYQRRQS 500
    LFEMVSELQG DPGYPLGRFG AAITALTDIN GDRLTDVAVG APLEEQGAVY 550
    IFNGKPGGLS PQPSQRIQGA QVFPGIRWFG RSIHGVKDLG GDRLADVVVG 600
    AEGRVVVLSS RPVVDVVTEL SFSPEEIPVH EVECSYSARE EQKHGVKLKA 650
    CFRIKPLTPQ FQGRLLANLS YTLQLDGHRM RSRGLFPDGS HELSGNTSIT 700
    PDKSCLDFHF HFPICIQDLI SPINVSLNFS LLEEEGTPRD QKGRAMQPIL 750
    RPSIHTVTKE IPFEKNCGED KKCEANLTLS SPARSGPLRL MSSASLAVEW 800
    TLSNSGEDAY WVRLDLDFPR GLSFRKVEML QPHSRMPVSC EELTEGSSLL 850
    TKTLKCNVSS PIFKAGQEVS LQVMFNTLLN SSWEDFVELN GTVHCENENS 900
    SLQEDNSAAT HIPVLYPVNI LTKEQENSTL YISFTPKGPK TQQVQHVYQV 950
    RIQPSAYDHN MPTLEALVGV PWPHSEDPIT YTWSVQTDPL VTCHSEDLKR 1000
    PSSEAEQPCL PGVQFRCPIV FRREILIQVT GTVELSKEIK ASSTLSLCSS 1050
    LSVSFNSSKH FHLYGSKASE AQVLVKVDLI HEKEMLHVYV LSGIGGLVLL 1100
    FLIFLALYKV GFFKRNLKEK MEADGGVPNG SPPEDTDPLA VPGEETKDMG 1150
    CLEPSGRVTR TKA 1163
    Length:1,163
    Mass (Da):128,328
    Last modified:July 27, 2011 - v2
    Checksum:iF19C786C4D33CC0C
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti972 – 9721W → R in AAA39426. (PubMed:2051027)Curated
    Sequence conflicti978 – 9781P → L in AAA39426. (PubMed:2051027)Curated
    Sequence conflicti1023 – 10231R → W in AAA39426. (PubMed:2051027)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M60778 mRNA. Translation: AAA39426.1.
    AC133494 Genomic DNA. No translation available.
    PIRiI56126.
    UniGeneiMm.1618.

    Genome annotation databases

    EnsembliENSMUST00000170971; ENSMUSP00000131847; ENSMUSG00000030830.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M60778 mRNA. Translation: AAA39426.1 .
    AC133494 Genomic DNA. No translation available.
    PIRi I56126.
    UniGenei Mm.1618.

    3D structure databases

    ProteinModelPortali P24063.
    SMRi P24063. Positions 24-1068, 1078-1154.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-35643N.
    IntActi P24063. 1 interaction.

    Chemistry

    BindingDBi P24063.
    ChEMBLi CHEMBL1075188.

    PTM databases

    PhosphoSitei P24063.

    Proteomic databases

    MaxQBi P24063.
    PaxDbi P24063.
    PRIDEi P24063.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000170971 ; ENSMUSP00000131847 ; ENSMUSG00000030830 .

    Organism-specific databases

    MGIi MGI:96606. Itgal.

    Phylogenomic databases

    eggNOGi NOG296688.
    GeneTreei ENSGT00750000117471.
    HOGENOMi HOG000113114.
    HOVERGENi HBG006188.

    Enzyme and pathway databases

    Reactomei REACT_202937. Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell.
    REACT_216309. Integrin cell surface interactions.
    REACT_225233. Cell surface interactions at the vascular wall.

    Miscellaneous databases

    PROi P24063.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P24063.
    Bgeei P24063.
    CleanExi MM_ITGAL.
    Genevestigatori P24063.

    Family and domain databases

    InterProi IPR013517. FG-GAP.
    IPR013519. Int_alpha_beta-p.
    IPR000413. Integrin_alpha.
    IPR013649. Integrin_alpha-2.
    IPR018184. Integrin_alpha_C_CS.
    IPR002035. VWF_A.
    [Graphical view ]
    Pfami PF01839. FG-GAP. 1 hit.
    PF00357. Integrin_alpha. 1 hit.
    PF08441. Integrin_alpha2. 1 hit.
    PF00092. VWA. 1 hit.
    [Graphical view ]
    PRINTSi PR01185. INTEGRINA.
    SMARTi SM00191. Int_alpha. 5 hits.
    SM00327. VWA. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53300. SSF53300. 1 hit.
    PROSITEi PS51470. FG_GAP. 7 hits.
    PS00242. INTEGRIN_ALPHA. 1 hit.
    PS50234. VWFA. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning of the murine lymphocyte function-associated molecule-1 alpha-subunit and its expression in COS cells."
      Kaufmann Y., Tseng E., Springer T.A.
      J. Immunol. 147:369-374(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    3. "Sequence homology of the LFA-1 and Mac-1 leukocyte adhesion glycoproteins and unexpected relation to leukocyte interferon."
      Springer T.A., Teplow D.B., Dreyer W.J.
      Nature 314:540-542(1985) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 24-42.

    Entry informationi

    Entry nameiITAL_MOUSE
    AccessioniPrimary (citable) accession number: P24063
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 1, 1992
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 130 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3