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Protein

Integrin alpha-L

Gene

Itgal

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4 (PubMed:2051027). Integrin alpha-L/beta-2 is also a receptor for F11R (By similarity). Involved in a variety of immune phenomena including leukocyte-endothelial cell interaction, cytotoxic T-cell mediated killing, and antibody dependent killing by granulocytes and monocytes. Contributes to natural killer cell cytotoxicity. Involved in leukocyte adhesion and transmigration of leukocytes including T-cells and neutrophils (PubMed:16234355, PubMed:24158516). Required for generation of common lymphoid progenitor cells in bone marrow, indicating the role in lymphopoiesis (PubMed:25108025). Integrin alpha-L/beta-2 in assosiation with ICAM3, contributes to apoptotic neutrophil phagocytosis by macrophages.By similarity4 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi466 – 4749Sequence analysis
Calcium bindingi528 – 5369Sequence analysis
Calcium bindingi588 – 5969Sequence analysis

GO - Molecular functioni

GO - Biological processi

  • activated T cell proliferation Source: MGI
  • cell-matrix adhesion Source: MGI
  • cell surface receptor signaling pathway Source: MGI
  • heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules Source: MGI
  • integrin-mediated signaling pathway Source: UniProtKB-KW
  • leukocyte cell-cell adhesion Source: MGI
  • phagocytosis Source: UniProtKB-KW
  • positive regulation of calcium-mediated signaling Source: MGI
  • positive regulation of cell-cell adhesion Source: MGI
  • positive regulation of T cell proliferation Source: MGI
  • receptor clustering Source: MGI
  • regulation of cell-cell adhesion Source: MGI
  • T cell activation via T cell receptor contact with antigen bound to MHC molecule on antigen presenting cell Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Integrin, Receptor

Keywords - Biological processi

Cell adhesion, Phagocytosis

Keywords - Ligandi

Calcium, Magnesium, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-198933. Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell.
R-MMU-202733. Cell surface interactions at the vascular wall.
R-MMU-216083. Integrin cell surface interactions.

Names & Taxonomyi

Protein namesi
Recommended name:
Integrin alpha-L
Alternative name(s):
CD11 antigen-like family member A
Leukocyte adhesion glycoprotein LFA-1 alpha chain
Short name:
LFA-1A
Leukocyte function-associated molecule 1 alpha chain
Lymphocyte antigen 15
Short name:
Ly-15
CD_antigen: CD11a
Gene namesi
Name:Itgal
Synonyms:Lfa-1, Ly-15
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:96606. Itgal.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini24 – 10871064ExtracellularSequence analysisAdd
BLAST
Transmembranei1088 – 110821HelicalSequence analysisAdd
BLAST
Topological domaini1109 – 116355CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • cell-cell junction Source: MGI
  • cell surface Source: MGI
  • external side of plasma membrane Source: MGI
  • extracellular exosome Source: MGI
  • immunological synapse Source: MGI
  • integrin alphaL-beta2 complex Source: MGI
  • membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Mice show decreased cellularity in thymus but not spleen, and impaired early T cell development (PubMed:25108025). Obese mutant mice show decreased total number of T-cells, lower levels of neutrophil elastase and reduced cytotoxic T-cell proliferation in adipose tissue, as well as improved glucose tolerance and insulin resistance in comparison to obese wild type mice (PubMed:24158516).2 Publications

Chemistry

ChEMBLiCHEMBL1075188.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 23231 PublicationAdd
BLAST
Chaini24 – 11631140Integrin alpha-LPRO_0000016293Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi70 ↔ 77By similarity
Glycosylationi86 – 861N-linked (GlcNAc...)Sequence analysis
Disulfide bondi108 ↔ 126By similarity
Disulfide bondi147 ↔ 199By similarity
Glycosylationi185 – 1851N-linked (GlcNAc...)Sequence analysis
Glycosylationi270 – 2701N-linked (GlcNAc...)Sequence analysis
Glycosylationi444 – 4441N-linked (GlcNAc...)Sequence analysis
Disulfide bondi651 ↔ 705By similarity
Glycosylationi668 – 6681N-linked (GlcNAc...)Sequence analysis
Glycosylationi696 – 6961N-linked (GlcNAc...)Sequence analysis
Glycosylationi724 – 7241N-linked (GlcNAc...)Sequence analysis
Glycosylationi728 – 7281N-linked (GlcNAc...)Sequence analysis
Disulfide bondi767 ↔ 773By similarity
Glycosylationi776 – 7761N-linked (GlcNAc...)Sequence analysis
Disulfide bondi840 ↔ 856By similarity
Glycosylationi857 – 8571N-linked (GlcNAc...)Sequence analysis
Glycosylationi880 – 8801N-linked (GlcNAc...)Sequence analysis
Glycosylationi890 – 8901N-linked (GlcNAc...)Sequence analysis
Glycosylationi899 – 8991N-linked (GlcNAc...)Sequence analysis
Glycosylationi927 – 9271N-linked (GlcNAc...)Sequence analysis
Disulfide bondi993 ↔ 1009By similarity
Disulfide bondi1017 ↔ 1048By similarity
Glycosylationi1056 – 10561N-linked (GlcNAc...)Sequence analysis

Post-translational modificationi

In resting T-cells, up to 40% of surface ITGAL is constitutively phosphorylated. Phosphorylation causes conformational changes needed for ligand binding and is necessary for the activation by some physiological agents.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

EPDiP24063.
MaxQBiP24063.
PaxDbiP24063.
PRIDEiP24063.

PTM databases

iPTMnetiP24063.
PhosphoSiteiP24063.
SwissPalmiP24063.

Expressioni

Tissue specificityi

Leukocytes.2 Publications

Gene expression databases

BgeeiENSMUSG00000030830.
CleanExiMM_ITGAL.
ExpressionAtlasiP24063. baseline and differential.
GenevisibleiP24063. MM.

Interactioni

Subunit structurei

Heterodimer of an alpha and a beta subunit. Alpha-L associates with beta-2. Interacts with THBD.By similarity

GO - Molecular functioni

Protein-protein interaction databases

DIPiDIP-35643N.
IntActiP24063. 1 interaction.
STRINGi10090.ENSMUSP00000101913.

Chemistry

BindingDBiP24063.

Structurei

3D structure databases

ProteinModelPortaliP24063.
SMRiP24063. Positions 152-330, 1078-1154.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati28 – 7952FG-GAP 1PROSITE-ProRule annotationAdd
BLAST
Repeati80 – 13859FG-GAP 2PROSITE-ProRule annotationAdd
BLAST
Domaini153 – 325173VWFAPROSITE-ProRule annotationAdd
BLAST
Repeati336 – 38752FG-GAP 3PROSITE-ProRule annotationAdd
BLAST
Repeati390 – 44354FG-GAP 4PROSITE-ProRule annotationAdd
BLAST
Repeati444 – 50461FG-GAP 5PROSITE-ProRule annotationAdd
BLAST
Repeati505 – 56157FG-GAP 6PROSITE-ProRule annotationAdd
BLAST
Repeati565 – 62561FG-GAP 7PROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi1111 – 11155GFFKR motif

Domaini

The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage. The I-domain is necessary and sufficient for interaction with ICAM1 and F11R.By similarity

Sequence similaritiesi

Belongs to the integrin alpha chain family.Curated
Contains 7 FG-GAP repeats.PROSITE-ProRule annotation
Contains 1 VWFA domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IPAP. Eukaryota.
ENOG410ZJIF. LUCA.
GeneTreeiENSGT00760000118782.
HOGENOMiHOG000113114.
HOVERGENiHBG006188.
InParanoidiP24063.

Family and domain databases

InterProiIPR013517. FG-GAP.
IPR013519. Int_alpha_beta-p.
IPR000413. Integrin_alpha.
IPR013649. Integrin_alpha-2.
IPR018184. Integrin_alpha_C_CS.
IPR032695. Integrin_dom.
IPR002035. VWF_A.
[Graphical view]
PfamiPF01839. FG-GAP. 1 hit.
PF00357. Integrin_alpha. 1 hit.
PF08441. Integrin_alpha2. 1 hit.
PF00092. VWA. 1 hit.
[Graphical view]
PRINTSiPR01185. INTEGRINA.
SMARTiSM00191. Int_alpha. 5 hits.
SM00327. VWA. 1 hit.
[Graphical view]
SUPFAMiSSF53300. SSF53300. 1 hit.
SSF69179. SSF69179. 2 hits.
PROSITEiPS51470. FG_GAP. 7 hits.
PS00242. INTEGRIN_ALPHA. 1 hit.
PS50234. VWFA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P24063-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSFRIAGPRL LLLGLQLFAK AWSYNLDTRP TQSFLAQAGR HFGYQVLQIE
60 70 80 90 100
DGVVVGAPGE GDNTGGLYHC RTSSEFCQPV SLHGSNHTSK YLGMTLATDA
110 120 130 140 150
AKGSLLACDP GLSRTCDQNT YLSGLCYLFP QSLEGPMLQN RPAYQECMKG
160 170 180 190 200
KVDLVFLFDG SQSLDRKDFE KILEFMKDVM RKLSNTSYQF AAVQFSTDCR
210 220 230 240 250
TEFTFLDYVK QNKNPDVLLG SVQPMFLLTN TFRAINYVVA HVFKEESGAR
260 270 280 290 300
PDATKVLVII TDGEASDKGN ISAAHDITRY IIGIGKHFVS VQKQKTLHIF
310 320 330 340 350
ASEPVEEFVK ILDTFEKLKD LFTDLQRRIY AIEGTNRQDL TSFNMELSSS
360 370 380 390 400
GISADLSKGH AVVGAVGAKD WAGGFLDLRE DLQGATFVGQ EPLTSDVRGG
410 420 430 440 450
YLGYTVAWMT SRSSRPLLAA GAPRYQHVGQ VLLFQAPEAG GRWNQTQKIE
460 470 480 490 500
GTQIGSYFGG ELCSVDLDQD GEAELLLIGA PLFFGEQRGG RVFTYQRRQS
510 520 530 540 550
LFEMVSELQG DPGYPLGRFG AAITALTDIN GDRLTDVAVG APLEEQGAVY
560 570 580 590 600
IFNGKPGGLS PQPSQRIQGA QVFPGIRWFG RSIHGVKDLG GDRLADVVVG
610 620 630 640 650
AEGRVVVLSS RPVVDVVTEL SFSPEEIPVH EVECSYSARE EQKHGVKLKA
660 670 680 690 700
CFRIKPLTPQ FQGRLLANLS YTLQLDGHRM RSRGLFPDGS HELSGNTSIT
710 720 730 740 750
PDKSCLDFHF HFPICIQDLI SPINVSLNFS LLEEEGTPRD QKGRAMQPIL
760 770 780 790 800
RPSIHTVTKE IPFEKNCGED KKCEANLTLS SPARSGPLRL MSSASLAVEW
810 820 830 840 850
TLSNSGEDAY WVRLDLDFPR GLSFRKVEML QPHSRMPVSC EELTEGSSLL
860 870 880 890 900
TKTLKCNVSS PIFKAGQEVS LQVMFNTLLN SSWEDFVELN GTVHCENENS
910 920 930 940 950
SLQEDNSAAT HIPVLYPVNI LTKEQENSTL YISFTPKGPK TQQVQHVYQV
960 970 980 990 1000
RIQPSAYDHN MPTLEALVGV PWPHSEDPIT YTWSVQTDPL VTCHSEDLKR
1010 1020 1030 1040 1050
PSSEAEQPCL PGVQFRCPIV FRREILIQVT GTVELSKEIK ASSTLSLCSS
1060 1070 1080 1090 1100
LSVSFNSSKH FHLYGSKASE AQVLVKVDLI HEKEMLHVYV LSGIGGLVLL
1110 1120 1130 1140 1150
FLIFLALYKV GFFKRNLKEK MEADGGVPNG SPPEDTDPLA VPGEETKDMG
1160
CLEPSGRVTR TKA
Length:1,163
Mass (Da):128,328
Last modified:July 27, 2011 - v2
Checksum:iF19C786C4D33CC0C
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti972 – 9721W → R in AAA39426 (PubMed:2051027).Curated
Sequence conflicti978 – 9781P → L in AAA39426 (PubMed:2051027).Curated
Sequence conflicti1023 – 10231R → W in AAA39426 (PubMed:2051027).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M60778 mRNA. Translation: AAA39426.1.
AC133494 Genomic DNA. No translation available.
PIRiI56126.
UniGeneiMm.1618.

Genome annotation databases

EnsembliENSMUST00000170971; ENSMUSP00000131847; ENSMUSG00000030830.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M60778 mRNA. Translation: AAA39426.1.
AC133494 Genomic DNA. No translation available.
PIRiI56126.
UniGeneiMm.1618.

3D structure databases

ProteinModelPortaliP24063.
SMRiP24063. Positions 152-330, 1078-1154.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-35643N.
IntActiP24063. 1 interaction.
STRINGi10090.ENSMUSP00000101913.

Chemistry

BindingDBiP24063.
ChEMBLiCHEMBL1075188.

PTM databases

iPTMnetiP24063.
PhosphoSiteiP24063.
SwissPalmiP24063.

Proteomic databases

EPDiP24063.
MaxQBiP24063.
PaxDbiP24063.
PRIDEiP24063.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000170971; ENSMUSP00000131847; ENSMUSG00000030830.

Organism-specific databases

MGIiMGI:96606. Itgal.

Phylogenomic databases

eggNOGiENOG410IPAP. Eukaryota.
ENOG410ZJIF. LUCA.
GeneTreeiENSGT00760000118782.
HOGENOMiHOG000113114.
HOVERGENiHBG006188.
InParanoidiP24063.

Enzyme and pathway databases

ReactomeiR-MMU-198933. Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell.
R-MMU-202733. Cell surface interactions at the vascular wall.
R-MMU-216083. Integrin cell surface interactions.

Miscellaneous databases

PROiP24063.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000030830.
CleanExiMM_ITGAL.
ExpressionAtlasiP24063. baseline and differential.
GenevisibleiP24063. MM.

Family and domain databases

InterProiIPR013517. FG-GAP.
IPR013519. Int_alpha_beta-p.
IPR000413. Integrin_alpha.
IPR013649. Integrin_alpha-2.
IPR018184. Integrin_alpha_C_CS.
IPR032695. Integrin_dom.
IPR002035. VWF_A.
[Graphical view]
PfamiPF01839. FG-GAP. 1 hit.
PF00357. Integrin_alpha. 1 hit.
PF08441. Integrin_alpha2. 1 hit.
PF00092. VWA. 1 hit.
[Graphical view]
PRINTSiPR01185. INTEGRINA.
SMARTiSM00191. Int_alpha. 5 hits.
SM00327. VWA. 1 hit.
[Graphical view]
SUPFAMiSSF53300. SSF53300. 1 hit.
SSF69179. SSF69179. 2 hits.
PROSITEiPS51470. FG_GAP. 7 hits.
PS00242. INTEGRIN_ALPHA. 1 hit.
PS50234. VWFA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiITAL_MOUSE
AccessioniPrimary (citable) accession number: P24063
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: July 27, 2011
Last modified: September 7, 2016
This is version 144 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.