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P24063 (ITAL_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 128. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Integrin alpha-L
Alternative name(s):
CD11 antigen-like family member A
Leukocyte adhesion glycoprotein LFA-1 alpha chain
Short name=LFA-1A
Leukocyte function-associated molecule 1 alpha chain
Lymphocyte antigen 15
Short name=Ly-15
CD_antigen=CD11a
Gene names
Name:Itgal
Synonyms:Lfa-1, Ly-15
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length1163 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. Is involved in a variety of immune phenomena including leukocyte-endothelial cell interaction, cytotoxic T-cell mediated killing, and antibody dependent killing by granulocytes and monocytes. Mice expressing a null mutation of the alpha-L subunit gene demonstrate impaired tumor rejection and impaired leukocytes recruitment.

Subunit structure

Heterodimer of an alpha and a beta subunit. Alpha-L associates with beta-2.

Subcellular location

Membrane; Single-pass type I membrane protein.

Tissue specificity

Leukocytes.

Domain

The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage.

Sequence similarities

Belongs to the integrin alpha chain family.

Contains 7 FG-GAP repeats.

Contains 1 VWFA domain.

Ontologies

Keywords
   Biological processCell adhesion
   Cellular componentMembrane
   DomainRepeat
Signal
Transmembrane
Transmembrane helix
   LigandCalcium
Magnesium
Metal-binding
   Molecular functionIntegrin
Receptor
   PTMDisulfide bond
Glycoprotein
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processactivated T cell proliferation

Inferred from mutant phenotype PubMed 15210787. Source: MGI

cell surface receptor signaling pathway

Inferred from direct assay PubMed 8117278. Source: MGI

integrin-mediated signaling pathway

Inferred from electronic annotation. Source: UniProtKB-KW

leukocyte cell-cell adhesion

Inferred from direct assay PubMed 15064761PubMed 8117278. Source: MGI

positive regulation of T cell proliferation

Inferred from mutant phenotype PubMed 1346270. Source: MGI

positive regulation of calcium-mediated signaling

Inferred from direct assay PubMed 8117278. Source: MGI

positive regulation of cell-cell adhesion

Inferred from mutant phenotype PubMed 1346270. Source: MGI

regulation of cell-cell adhesion

Inferred from direct assay PubMed 8117278. Source: MGI

   Cellular_componentcell-cell junction

Inferred from direct assay PubMed 23793062. Source: MGI

external side of plasma membrane

Inferred from direct assay PubMed 8043862PubMed 8557754. Source: MGI

immunological synapse

Inferred from direct assay PubMed 15064761. Source: MGI

integrin complex

Inferred from electronic annotation. Source: InterPro

   Molecular_functionmetal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2323 Ref.3
Chain24 – 11631140Integrin alpha-L
PRO_0000016293

Regions

Topological domain24 – 10871064Extracellular Potential
Transmembrane1088 – 110821Helical; Potential
Topological domain1109 – 116355Cytoplasmic Potential
Repeat28 – 7952FG-GAP 1
Repeat80 – 13859FG-GAP 2
Domain153 – 325173VWFA
Repeat336 – 38752FG-GAP 3
Repeat390 – 44354FG-GAP 4
Repeat444 – 50461FG-GAP 5
Repeat505 – 56157FG-GAP 6
Repeat565 – 62561FG-GAP 7
Calcium binding466 – 4749 Potential
Calcium binding528 – 5369 Potential
Calcium binding588 – 5969 Potential
Motif1111 – 11155GFFKR motif

Amino acid modifications

Glycosylation861N-linked (GlcNAc...) Potential
Glycosylation1851N-linked (GlcNAc...) Potential
Glycosylation2701N-linked (GlcNAc...) Potential
Glycosylation4441N-linked (GlcNAc...) Potential
Glycosylation6681N-linked (GlcNAc...) Potential
Glycosylation6961N-linked (GlcNAc...) Potential
Glycosylation7241N-linked (GlcNAc...) Potential
Glycosylation7281N-linked (GlcNAc...) Potential
Glycosylation7761N-linked (GlcNAc...) Potential
Glycosylation8571N-linked (GlcNAc...) Potential
Glycosylation8801N-linked (GlcNAc...) Potential
Glycosylation8901N-linked (GlcNAc...) Potential
Glycosylation8991N-linked (GlcNAc...) Potential
Glycosylation9271N-linked (GlcNAc...) Potential
Glycosylation10561N-linked (GlcNAc...) Potential
Disulfide bond70 ↔ 77 By similarity
Disulfide bond108 ↔ 126 By similarity
Disulfide bond147 ↔ 199 By similarity
Disulfide bond651 ↔ 705 By similarity
Disulfide bond767 ↔ 773 By similarity
Disulfide bond840 ↔ 856 By similarity
Disulfide bond993 ↔ 1009 By similarity
Disulfide bond1017 ↔ 1048 By similarity

Experimental info

Sequence conflict9721W → R in AAA39426. Ref.1
Sequence conflict9781P → L in AAA39426. Ref.1
Sequence conflict10231R → W in AAA39426. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P24063 [UniParc].

Last modified July 27, 2011. Version 2.
Checksum: F19C786C4D33CC0C

FASTA1,163128,328
        10         20         30         40         50         60 
MSFRIAGPRL LLLGLQLFAK AWSYNLDTRP TQSFLAQAGR HFGYQVLQIE DGVVVGAPGE 

        70         80         90        100        110        120 
GDNTGGLYHC RTSSEFCQPV SLHGSNHTSK YLGMTLATDA AKGSLLACDP GLSRTCDQNT 

       130        140        150        160        170        180 
YLSGLCYLFP QSLEGPMLQN RPAYQECMKG KVDLVFLFDG SQSLDRKDFE KILEFMKDVM 

       190        200        210        220        230        240 
RKLSNTSYQF AAVQFSTDCR TEFTFLDYVK QNKNPDVLLG SVQPMFLLTN TFRAINYVVA 

       250        260        270        280        290        300 
HVFKEESGAR PDATKVLVII TDGEASDKGN ISAAHDITRY IIGIGKHFVS VQKQKTLHIF 

       310        320        330        340        350        360 
ASEPVEEFVK ILDTFEKLKD LFTDLQRRIY AIEGTNRQDL TSFNMELSSS GISADLSKGH 

       370        380        390        400        410        420 
AVVGAVGAKD WAGGFLDLRE DLQGATFVGQ EPLTSDVRGG YLGYTVAWMT SRSSRPLLAA 

       430        440        450        460        470        480 
GAPRYQHVGQ VLLFQAPEAG GRWNQTQKIE GTQIGSYFGG ELCSVDLDQD GEAELLLIGA 

       490        500        510        520        530        540 
PLFFGEQRGG RVFTYQRRQS LFEMVSELQG DPGYPLGRFG AAITALTDIN GDRLTDVAVG 

       550        560        570        580        590        600 
APLEEQGAVY IFNGKPGGLS PQPSQRIQGA QVFPGIRWFG RSIHGVKDLG GDRLADVVVG 

       610        620        630        640        650        660 
AEGRVVVLSS RPVVDVVTEL SFSPEEIPVH EVECSYSARE EQKHGVKLKA CFRIKPLTPQ 

       670        680        690        700        710        720 
FQGRLLANLS YTLQLDGHRM RSRGLFPDGS HELSGNTSIT PDKSCLDFHF HFPICIQDLI 

       730        740        750        760        770        780 
SPINVSLNFS LLEEEGTPRD QKGRAMQPIL RPSIHTVTKE IPFEKNCGED KKCEANLTLS 

       790        800        810        820        830        840 
SPARSGPLRL MSSASLAVEW TLSNSGEDAY WVRLDLDFPR GLSFRKVEML QPHSRMPVSC 

       850        860        870        880        890        900 
EELTEGSSLL TKTLKCNVSS PIFKAGQEVS LQVMFNTLLN SSWEDFVELN GTVHCENENS 

       910        920        930        940        950        960 
SLQEDNSAAT HIPVLYPVNI LTKEQENSTL YISFTPKGPK TQQVQHVYQV RIQPSAYDHN 

       970        980        990       1000       1010       1020 
MPTLEALVGV PWPHSEDPIT YTWSVQTDPL VTCHSEDLKR PSSEAEQPCL PGVQFRCPIV 

      1030       1040       1050       1060       1070       1080 
FRREILIQVT GTVELSKEIK ASSTLSLCSS LSVSFNSSKH FHLYGSKASE AQVLVKVDLI 

      1090       1100       1110       1120       1130       1140 
HEKEMLHVYV LSGIGGLVLL FLIFLALYKV GFFKRNLKEK MEADGGVPNG SPPEDTDPLA 

      1150       1160 
VPGEETKDMG CLEPSGRVTR TKA 

« Hide

References

« Hide 'large scale' references
[1]"Cloning of the murine lymphocyte function-associated molecule-1 alpha-subunit and its expression in COS cells."
Kaufmann Y., Tseng E., Springer T.A.
J. Immunol. 147:369-374(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[3]"Sequence homology of the LFA-1 and Mac-1 leukocyte adhesion glycoproteins and unexpected relation to leukocyte interferon."
Springer T.A., Teplow D.B., Dreyer W.J.
Nature 314:540-542(1985) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 24-42.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M60778 mRNA. Translation: AAA39426.1.
AC133494 Genomic DNA. No translation available.
PIRI56126.
UniGeneMm.1618.

3D structure databases

ProteinModelPortalP24063.
SMRP24063. Positions 24-1068, 1078-1154.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-35643N.
IntActP24063. 1 interaction.

Chemistry

BindingDBP24063.
ChEMBLCHEMBL1075188.

PTM databases

PhosphoSiteP24063.

Proteomic databases

MaxQBP24063.
PaxDbP24063.
PRIDEP24063.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000170971; ENSMUSP00000131847; ENSMUSG00000030830.

Organism-specific databases

MGIMGI:96606. Itgal.

Phylogenomic databases

eggNOGNOG296688.
GeneTreeENSGT00750000117471.
HOGENOMHOG000113114.
HOVERGENHBG006188.

Gene expression databases

ArrayExpressP24063.
BgeeP24063.
CleanExMM_ITGAL.
GenevestigatorP24063.

Family and domain databases

InterProIPR013517. FG-GAP.
IPR013519. Int_alpha_beta-p.
IPR000413. Integrin_alpha.
IPR013649. Integrin_alpha-2.
IPR018184. Integrin_alpha_C_CS.
IPR002035. VWF_A.
[Graphical view]
PfamPF01839. FG-GAP. 1 hit.
PF00357. Integrin_alpha. 1 hit.
PF08441. Integrin_alpha2. 1 hit.
PF00092. VWA. 1 hit.
[Graphical view]
PRINTSPR01185. INTEGRINA.
SMARTSM00191. Int_alpha. 5 hits.
SM00327. VWA. 1 hit.
[Graphical view]
SUPFAMSSF53300. SSF53300. 1 hit.
PROSITEPS51470. FG_GAP. 7 hits.
PS00242. INTEGRIN_ALPHA. 1 hit.
PS50234. VWFA. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROP24063.
SOURCESearch...

Entry information

Entry nameITAL_MOUSE
AccessionPrimary (citable) accession number: P24063
Entry history
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: July 27, 2011
Last modified: June 11, 2014
This is version 128 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot