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P24040 (NIRS_PSEST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 87. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Nitrite reductase

EC=1.7.2.1
Alternative name(s):
Cytochrome cd1
Cytochrome oxidase
Hydroxylamine reductase
EC=1.7.99.1
Gene names
Name:nirS
OrganismPseudomonas stutzeri (Pseudomonas perfectomarina)
Taxonomic identifier316 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

Protein attributes

Sequence length560 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

Nitric oxide + H2O + ferricytochrome c = nitrite + ferrocytochrome c + 2 H+.

NH3 + H2O + acceptor = hydroxylamine + reduced acceptor.

Cofactor

Binds 2 heme groups per subunit By similarity.

Subunit structure

Homodimer in solution.

Subcellular location

Periplasm.

Induction

By anaerobic conditions. Induced by nitrite, repressed by nitrate, and inhibited by oxygen.

Sequence similarities

Contains 1 cytochrome c domain.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2626
Chain27 – 560534Nitrite reductase
PRO_0000006577

Regions

Domain30 – 12697Cytochrome c
Region27 – 293N-terminal tail
Region127 – 560434D1-heme domain

Sites

Metal binding511Iron (heme axial ligand) By similarity
Metal binding1011Iron (heme axial ligand) By similarity
Metal binding1931Iron (heme D1 proximal ligand) By similarity
Binding site471Heme (covalent) By similarity
Binding site501Heme (covalent) By similarity

Sequences

Sequence LengthMass (Da)Tools
P24040 [UniParc].

Last modified March 1, 1992. Version 1.
Checksum: 476259C35FBFA7A0

FASTA56061,993
        10         20         30         40         50         60 
MSNVGKPILA GLIAGLSLLG LAVAQAAAPE MTAEEKEASK QIYFERCAGC HGVLRKGATG 

        70         80         90        100        110        120 
KNLEPHWSKT EADGKKTEGG TLNLGTKRLE NIIAYGTEGG MVNYDDILTK EEINMMARYI 

       130        140        150        160        170        180 
QHTPDIPPEF SLQDMKDSWN LIVPVEKRVT KQMNKINLQN VFAVTLRDAG KLALIDGDTH 

       190        200        210        220        230        240 
KIWKVLESGY AVHISRMSAS GRYVYTTGRD GLTTIIDLWP EEPMTVATVR FGSDMRSVDV 

       250        260        270        280        290        300 
SKFEGYEDKY LIGGTYWPPQ YSIVDGLTLE PIKVVSTRGQ TVDGEYHPEP RVASIVASHI 

       310        320        330        340        350        360 
KPEWVVNVKE TGQIILVDYT DLKNLKTTTI ESAKFLHDGG WDYSKRYFMV AANASNKVAA 

       370        380        390        400        410        420 
VDTKTGKLAA LIDTAKIPHP GRGANFVHPQ FGPVWSTGHL GDDVVSLIST PSEESKYAKY 

       430        440        450        460        470        480 
KEHNWKVVQE LKMPGAGNLF VKTHPKSKHF WADAPMNPER EVAESVYVFD MNDLSKAPIQ 

       490        500        510        520        530        540 
LNVAKDSGLP ESKAIRRAVQ PEYNKAGDEV WISLWGGKTD QSAIVIYDDK TLKLKRVITD 

       550        560 
PAVVTPTGKF NVFNTMNDVY 

« Hide

References

[1]"The nirSTBM region coding for cytochrome cd1-dependent nitrite respiration of Pseudomonas stutzeri consists of a cluster of mono-, di-, and tetraheme proteins."
Juengst A., Wakabayashi S., Matsubara H., Zumft W.G.
FEBS Lett. 279:205-209(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
Strain: ATCC 14405 / 218 / ZoBell.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X53676 Genomic DNA. Translation: CAA40150.1.
PIROSPSZ. S13613.

3D structure databases

ProteinModelPortalP24040.
SMRP24040. Positions 23-560.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

BioCycMetaCyc:MONOMER-201.

Family and domain databases

Gene3D1.10.760.10. 1 hit.
2.130.10.10. 1 hit.
2.140.10.20. 1 hit.
InterProIPR009056. Cyt_c-like_dom.
IPR003143. Cyt_cd1_C.
IPR011048. Haem_d1.
IPR015943. WD40/YVTN_repeat-like_dom.
[Graphical view]
PfamPF13442. Cytochrome_CBB3. 1 hit.
[Graphical view]
SUPFAMSSF46626. SSF46626. 1 hit.
SSF51004. SSF51004. 1 hit.
PROSITEPS51007. CYTC. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNIRS_PSEST
AccessionPrimary (citable) accession number: P24040
Entry history
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: March 1, 1992
Last modified: April 16, 2014
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families