Reviewed, UniProtKB/Swiss-Prot P24040 (NIRS_PSEST)

Names and origin

Protein names

Recommended name:
    Nitrite reductase
    EC=1.7.2.1
Alternative name(s):
    Cytochrome cd1
    Cytochrome oxidase
    Hydroxylamine reductase
    EC=1.7.99.1

Gene names

Name:

nirS

Organism

Pseudomonas stutzeri (Pseudomonas perfectomarina)

Taxonomic identifier

316 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	560 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Nitric oxide + H2O + ferricytochrome c = nitrite + ferrocytochrome c + 2 H+. NH3 + H2O + acceptor = hydroxylamine + reduced acceptor.
Cofactor	Binds 2 heme groups per subunit By similarity.
Subunit structure	Homodimer in solution.
Subcellular location	Periplasm.
Induction	By anaerobic conditions. Induced by nitrite, repressed by nitrate, and inhibited by oxygen.
Sequence similarities	Contains 1 cytochrome c domain.

Ontologies

Keywords
Biological process	Electron transport Transport
Cellular component	Periplasm
Domain	Signal
Ligand	Heme Iron Metal-binding
Molecular function	Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	periplasmic space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro hydroxylamine reductase activity Inferred from electronic annotation. Source: EC nitrite reductase (NO-forming) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 26	26
	Chain	27 – 560	534	Nitrite reductase		PRO_0000006577
Regions
	Domain	30 – 126	97	Cytochrome c
	Region	27 – 29	3	N-terminal tail
	Region	127 – 560	434	D1-heme domain
Sites
	Metal binding	51	1	Iron (heme axial ligand) By similarity
	Metal binding	101	1	Iron (heme axial ligand) By similarity
	Metal binding	193	1	Iron (heme D1 proximal ligand) By similarity
	Binding site	47	1	Heme (covalent) By similarity
	Binding site	50	1	Heme (covalent) By similarity

Sequences

	Sequence		Length	Mass (Da)
	P24040-1 [UniParc]. Last modified March 1, 1992. Version 1. Checksum: 476259C35FBFA7A0	FASTA	560	61,993
	10 20 30 40 50 60 MSNVGKPILA GLIAGLSLLG LAVAQAAAPE MTAEEKEASK QIYFERCAGC HGVLRKGATG 70 80 90 100 110 120 KNLEPHWSKT EADGKKTEGG TLNLGTKRLE NIIAYGTEGG MVNYDDILTK EEINMMARYI 130 140 150 160 170 180 QHTPDIPPEF SLQDMKDSWN LIVPVEKRVT KQMNKINLQN VFAVTLRDAG KLALIDGDTH 190 200 210 220 230 240 KIWKVLESGY AVHISRMSAS GRYVYTTGRD GLTTIIDLWP EEPMTVATVR FGSDMRSVDV 250 260 270 280 290 300 SKFEGYEDKY LIGGTYWPPQ YSIVDGLTLE PIKVVSTRGQ TVDGEYHPEP RVASIVASHI 310 320 330 340 350 360 KPEWVVNVKE TGQIILVDYT DLKNLKTTTI ESAKFLHDGG WDYSKRYFMV AANASNKVAA 370 380 390 400 410 420 VDTKTGKLAA LIDTAKIPHP GRGANFVHPQ FGPVWSTGHL GDDVVSLIST PSEESKYAKY 430 440 450 460 470 480 KEHNWKVVQE LKMPGAGNLF VKTHPKSKHF WADAPMNPER EVAESVYVFD MNDLSKAPIQ 490 500 510 520 530 540 LNVAKDSGLP ESKAIRRAVQ PEYNKAGDEV WISLWGGKTD QSAIVIYDDK TLKLKRVITD 550 560 PAVVTPTGKF NVFNTMNDVY

References

[1]

"The nirSTBM region coding for cytochrome cd1-dependent nitrite respiration of Pseudomonas stutzeri consists of a cluster of mono-, di-, and tetraheme proteins." Juengst A., Wakabayashi S., Matsubara H., Zumft W.G. FEBS Lett. 279:205-209(1991) [PubMed: 2001732] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE. Strain: ATCC 14405 / 218 / ZoBell.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X53676 Genomic DNA. Translation: CAA40150.1.
PIR	OSPSZ. S13613.
3D structure databases
HSSP	HSSP built from PDB template 1GJQ based on UniProtKB P24474.
ModBase	Search...
Enzyme and pathway databases
BioCyc	MetaCyc:MON-201.
BRENDA	1.7.2.1. 421. 1.7.99.1. 421.
Family and domain databases
InterPro	IPR009056. Cyt_c_monohaem. IPR003143. Cyt_d1_haem. [Graphical view]
Gene3D	G3DSA:2.140.10.20. Cyt_d1_haem. 1 hit. G3DSA:1.10.760.10. Cytochrome_c_R. 1 hit.
Pfam	PF02239. Cytochrom_D1. 1 hit. [Graphical view]
PROSITE	PS51007. CYTC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NIRS_PSEST

Accession

Primary (citable) accession number: P24040

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 1992
Last sequence update:	March 1, 1992
Last modified:	June 16, 2009
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)