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P24040

- NIRS_PSEST

UniProt

P24040 - NIRS_PSEST

Protein

Nitrite reductase

Gene

nirS

Organism
Pseudomonas stutzeri (Pseudomonas perfectomarina)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 88 (01 Oct 2014)
      Sequence version 1 (01 Mar 1992)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Nitric oxide + H2O + ferricytochrome c = nitrite + ferrocytochrome c + 2 H+.
    NH3 + H2O + acceptor = hydroxylamine + reduced acceptor.

    Cofactori

    Binds 2 heme groups per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei47 – 471Heme (covalent)PROSITE-ProRule annotation
    Binding sitei50 – 501Heme (covalent)PROSITE-ProRule annotation
    Metal bindingi51 – 511Iron (heme axial ligand)PROSITE-ProRule annotation
    Metal bindingi101 – 1011Iron (heme axial ligand)PROSITE-ProRule annotation
    Metal bindingi193 – 1931Iron (heme D1 proximal ligand)By similarity

    GO - Molecular functioni

    1. electron carrier activity Source: InterPro
    2. heme binding Source: InterPro
    3. hydroxylamine reductase activity Source: UniProtKB-EC
    4. metal ion binding Source: UniProtKB-KW
    5. nitrite reductase (NO-forming) activity Source: UniProtKB-EC

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Electron transport, Transport

    Keywords - Ligandi

    Heme, Iron, Metal-binding

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-201.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Nitrite reductase (EC:1.7.2.1)
    Alternative name(s):
    Cytochrome cd1
    Cytochrome oxidase
    Hydroxylamine reductase (EC:1.7.99.1)
    Gene namesi
    Name:nirS
    OrganismiPseudomonas stutzeri (Pseudomonas perfectomarina)
    Taxonomic identifieri316 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

    Subcellular locationi

    GO - Cellular componenti

    1. periplasmic space Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Periplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2626Add
    BLAST
    Chaini27 – 560534Nitrite reductasePRO_0000006577Add
    BLAST

    Expressioni

    Inductioni

    By anaerobic conditions. Induced by nitrite, repressed by nitrate, and inhibited by oxygen.

    Interactioni

    Subunit structurei

    Homodimer in solution.

    Structurei

    3D structure databases

    ProteinModelPortaliP24040.
    SMRiP24040. Positions 23-560.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini30 – 12697Cytochrome cPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni27 – 293N-terminal tail
    Regioni127 – 560434D1-heme domainAdd
    BLAST

    Sequence similaritiesi

    Contains 1 cytochrome c domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal

    Family and domain databases

    Gene3Di1.10.760.10. 1 hit.
    2.130.10.10. 1 hit.
    2.140.10.20. 1 hit.
    InterProiIPR009056. Cyt_c-like_dom.
    IPR003143. Cyt_cd1_C.
    IPR011048. Haem_d1.
    IPR015943. WD40/YVTN_repeat-like_dom.
    [Graphical view]
    PfamiPF13442. Cytochrome_CBB3. 1 hit.
    [Graphical view]
    SUPFAMiSSF46626. SSF46626. 1 hit.
    SSF51004. SSF51004. 1 hit.
    PROSITEiPS51007. CYTC. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P24040-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSNVGKPILA GLIAGLSLLG LAVAQAAAPE MTAEEKEASK QIYFERCAGC    50
    HGVLRKGATG KNLEPHWSKT EADGKKTEGG TLNLGTKRLE NIIAYGTEGG 100
    MVNYDDILTK EEINMMARYI QHTPDIPPEF SLQDMKDSWN LIVPVEKRVT 150
    KQMNKINLQN VFAVTLRDAG KLALIDGDTH KIWKVLESGY AVHISRMSAS 200
    GRYVYTTGRD GLTTIIDLWP EEPMTVATVR FGSDMRSVDV SKFEGYEDKY 250
    LIGGTYWPPQ YSIVDGLTLE PIKVVSTRGQ TVDGEYHPEP RVASIVASHI 300
    KPEWVVNVKE TGQIILVDYT DLKNLKTTTI ESAKFLHDGG WDYSKRYFMV 350
    AANASNKVAA VDTKTGKLAA LIDTAKIPHP GRGANFVHPQ FGPVWSTGHL 400
    GDDVVSLIST PSEESKYAKY KEHNWKVVQE LKMPGAGNLF VKTHPKSKHF 450
    WADAPMNPER EVAESVYVFD MNDLSKAPIQ LNVAKDSGLP ESKAIRRAVQ 500
    PEYNKAGDEV WISLWGGKTD QSAIVIYDDK TLKLKRVITD PAVVTPTGKF 550
    NVFNTMNDVY 560
    Length:560
    Mass (Da):61,993
    Last modified:March 1, 1992 - v1
    Checksum:i476259C35FBFA7A0
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X53676 Genomic DNA. Translation: CAA40150.1.
    PIRiS13613. OSPSZ.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X53676 Genomic DNA. Translation: CAA40150.1 .
    PIRi S13613. OSPSZ.

    3D structure databases

    ProteinModelPortali P24040.
    SMRi P24040. Positions 23-560.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    BioCyci MetaCyc:MONOMER-201.

    Family and domain databases

    Gene3Di 1.10.760.10. 1 hit.
    2.130.10.10. 1 hit.
    2.140.10.20. 1 hit.
    InterProi IPR009056. Cyt_c-like_dom.
    IPR003143. Cyt_cd1_C.
    IPR011048. Haem_d1.
    IPR015943. WD40/YVTN_repeat-like_dom.
    [Graphical view ]
    Pfami PF13442. Cytochrome_CBB3. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46626. SSF46626. 1 hit.
    SSF51004. SSF51004. 1 hit.
    PROSITEi PS51007. CYTC. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The nirSTBM region coding for cytochrome cd1-dependent nitrite respiration of Pseudomonas stutzeri consists of a cluster of mono-, di-, and tetraheme proteins."
      Juengst A., Wakabayashi S., Matsubara H., Zumft W.G.
      FEBS Lett. 279:205-209(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
      Strain: ATCC 14405 / 218 / ZoBell.

    Entry informationi

    Entry nameiNIRS_PSEST
    AccessioniPrimary (citable) accession number: P24040
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 1, 1992
    Last sequence update: March 1, 1992
    Last modified: October 1, 2014
    This is version 88 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3