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P24040

- NIRS_PSEST

UniProt

P24040 - NIRS_PSEST

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Protein

Nitrite reductase

Gene

nirS

Organism
Pseudomonas stutzeri (Pseudomonas perfectomarina)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

Nitric oxide + H2O + ferricytochrome c = nitrite + ferrocytochrome c + 2 H+.
NH3 + H2O + acceptor = hydroxylamine + reduced acceptor.

Cofactori

hemeBy similarityNote: Binds 2 heme groups per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei47 – 471Heme (covalent)PROSITE-ProRule annotation
Binding sitei50 – 501Heme (covalent)PROSITE-ProRule annotation
Metal bindingi51 – 511Iron (heme axial ligand)PROSITE-ProRule annotation
Metal bindingi101 – 1011Iron (heme axial ligand)PROSITE-ProRule annotation
Metal bindingi193 – 1931Iron (heme D1 proximal ligand)By similarity

GO - Molecular functioni

  1. electron carrier activity Source: InterPro
  2. heme binding Source: InterPro
  3. hydroxylamine reductase activity Source: UniProtKB-EC
  4. metal ion binding Source: UniProtKB-KW
  5. nitrite reductase (NO-forming) activity Source: UniProtKB-EC
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Transport

Keywords - Ligandi

Heme, Iron, Metal-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-201.

Names & Taxonomyi

Protein namesi
Recommended name:
Nitrite reductase (EC:1.7.2.1)
Alternative name(s):
Cytochrome cd1
Cytochrome oxidase
Hydroxylamine reductase (EC:1.7.99.1)
Gene namesi
Name:nirS
OrganismiPseudomonas stutzeri (Pseudomonas perfectomarina)
Taxonomic identifieri316 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

Subcellular locationi

GO - Cellular componenti

  1. periplasmic space Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2626Add
BLAST
Chaini27 – 560534Nitrite reductasePRO_0000006577Add
BLAST

Expressioni

Inductioni

By anaerobic conditions. Induced by nitrite, repressed by nitrate, and inhibited by oxygen.

Interactioni

Subunit structurei

Homodimer in solution.

Structurei

3D structure databases

ProteinModelPortaliP24040.
SMRiP24040. Positions 23-560.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini30 – 12697Cytochrome cPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni27 – 293N-terminal tail
Regioni127 – 560434D1-heme domainAdd
BLAST

Sequence similaritiesi

Contains 1 cytochrome c domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Family and domain databases

Gene3Di1.10.760.10. 1 hit.
2.130.10.10. 1 hit.
2.140.10.20. 1 hit.
InterProiIPR009056. Cyt_c-like_dom.
IPR003143. Cyt_cd1_C.
IPR011048. Haem_d1.
IPR015943. WD40/YVTN_repeat-like_dom.
[Graphical view]
PfamiPF13442. Cytochrome_CBB3. 1 hit.
[Graphical view]
SUPFAMiSSF46626. SSF46626. 1 hit.
SSF51004. SSF51004. 1 hit.
PROSITEiPS51007. CYTC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P24040-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSNVGKPILA GLIAGLSLLG LAVAQAAAPE MTAEEKEASK QIYFERCAGC
60 70 80 90 100
HGVLRKGATG KNLEPHWSKT EADGKKTEGG TLNLGTKRLE NIIAYGTEGG
110 120 130 140 150
MVNYDDILTK EEINMMARYI QHTPDIPPEF SLQDMKDSWN LIVPVEKRVT
160 170 180 190 200
KQMNKINLQN VFAVTLRDAG KLALIDGDTH KIWKVLESGY AVHISRMSAS
210 220 230 240 250
GRYVYTTGRD GLTTIIDLWP EEPMTVATVR FGSDMRSVDV SKFEGYEDKY
260 270 280 290 300
LIGGTYWPPQ YSIVDGLTLE PIKVVSTRGQ TVDGEYHPEP RVASIVASHI
310 320 330 340 350
KPEWVVNVKE TGQIILVDYT DLKNLKTTTI ESAKFLHDGG WDYSKRYFMV
360 370 380 390 400
AANASNKVAA VDTKTGKLAA LIDTAKIPHP GRGANFVHPQ FGPVWSTGHL
410 420 430 440 450
GDDVVSLIST PSEESKYAKY KEHNWKVVQE LKMPGAGNLF VKTHPKSKHF
460 470 480 490 500
WADAPMNPER EVAESVYVFD MNDLSKAPIQ LNVAKDSGLP ESKAIRRAVQ
510 520 530 540 550
PEYNKAGDEV WISLWGGKTD QSAIVIYDDK TLKLKRVITD PAVVTPTGKF
560
NVFNTMNDVY
Length:560
Mass (Da):61,993
Last modified:March 1, 1992 - v1
Checksum:i476259C35FBFA7A0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X53676 Genomic DNA. Translation: CAA40150.1.
PIRiS13613. OSPSZ.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X53676 Genomic DNA. Translation: CAA40150.1 .
PIRi S13613. OSPSZ.

3D structure databases

ProteinModelPortali P24040.
SMRi P24040. Positions 23-560.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

BioCyci MetaCyc:MONOMER-201.

Family and domain databases

Gene3Di 1.10.760.10. 1 hit.
2.130.10.10. 1 hit.
2.140.10.20. 1 hit.
InterProi IPR009056. Cyt_c-like_dom.
IPR003143. Cyt_cd1_C.
IPR011048. Haem_d1.
IPR015943. WD40/YVTN_repeat-like_dom.
[Graphical view ]
Pfami PF13442. Cytochrome_CBB3. 1 hit.
[Graphical view ]
SUPFAMi SSF46626. SSF46626. 1 hit.
SSF51004. SSF51004. 1 hit.
PROSITEi PS51007. CYTC. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The nirSTBM region coding for cytochrome cd1-dependent nitrite respiration of Pseudomonas stutzeri consists of a cluster of mono-, di-, and tetraheme proteins."
    Juengst A., Wakabayashi S., Matsubara H., Zumft W.G.
    FEBS Lett. 279:205-209(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
    Strain: ATCC 14405 / 218 / ZoBell.

Entry informationi

Entry nameiNIRS_PSEST
AccessioniPrimary (citable) accession number: P24040
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: March 1, 1992
Last modified: November 26, 2014
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3