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Protein

Nitrite reductase

Gene

nirS

Organism
Pseudomonas stutzeri (Pseudomonas perfectomarina)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Nitric oxide + H2O + ferricytochrome c = nitrite + ferrocytochrome c + 2 H+.
NH3 + H2O + acceptor = hydroxylamine + reduced acceptor.

Cofactori

hemeBy similarityNote: Binds 2 heme groups per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei47Heme (covalent)PROSITE-ProRule annotation1
Binding sitei50Heme (covalent)PROSITE-ProRule annotation1
Metal bindingi51Iron (heme axial ligand)PROSITE-ProRule annotation1
Metal bindingi101Iron (heme axial ligand)PROSITE-ProRule annotation1
Metal bindingi193Iron (heme D1 proximal ligand)By similarity1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Transport

Keywords - Ligandi

Heme, Iron, Metal-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-201.
BRENDAi1.7.2.1. 5158.

Names & Taxonomyi

Protein namesi
Recommended name:
Nitrite reductase (EC:1.7.2.1)
Alternative name(s):
Cytochrome cd1
Cytochrome oxidase
Hydroxylamine reductase (EC:1.7.99.1)
Gene namesi
Name:nirS
OrganismiPseudomonas stutzeri (Pseudomonas perfectomarina)
Taxonomic identifieri316 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 26Add BLAST26
ChainiPRO_000000657727 – 560Nitrite reductaseAdd BLAST534

Expressioni

Inductioni

By anaerobic conditions. Induced by nitrite, repressed by nitrate, and inhibited by oxygen.

Interactioni

Subunit structurei

Homodimer in solution.

Structurei

3D structure databases

ProteinModelPortaliP24040.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini30 – 126Cytochrome cPROSITE-ProRule annotationAdd BLAST97

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni27 – 29N-terminal tail3
Regioni127 – 560D1-heme domainAdd BLAST434

Sequence similaritiesi

Contains 1 cytochrome c domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

KOiK15864.

Family and domain databases

Gene3Di1.10.760.10. 1 hit.
2.130.10.10. 1 hit.
2.140.10.20. 1 hit.
InterProiIPR009056. Cyt_c-like_dom.
IPR003143. Cyt_cd1_C.
IPR011048. Haem_d1.
IPR015943. WD40/YVTN_repeat-like_dom.
[Graphical view]
PfamiPF13442. Cytochrome_CBB3. 1 hit.
[Graphical view]
SUPFAMiSSF46626. SSF46626. 1 hit.
SSF51004. SSF51004. 1 hit.
PROSITEiPS51007. CYTC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P24040-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSNVGKPILA GLIAGLSLLG LAVAQAAAPE MTAEEKEASK QIYFERCAGC
60 70 80 90 100
HGVLRKGATG KNLEPHWSKT EADGKKTEGG TLNLGTKRLE NIIAYGTEGG
110 120 130 140 150
MVNYDDILTK EEINMMARYI QHTPDIPPEF SLQDMKDSWN LIVPVEKRVT
160 170 180 190 200
KQMNKINLQN VFAVTLRDAG KLALIDGDTH KIWKVLESGY AVHISRMSAS
210 220 230 240 250
GRYVYTTGRD GLTTIIDLWP EEPMTVATVR FGSDMRSVDV SKFEGYEDKY
260 270 280 290 300
LIGGTYWPPQ YSIVDGLTLE PIKVVSTRGQ TVDGEYHPEP RVASIVASHI
310 320 330 340 350
KPEWVVNVKE TGQIILVDYT DLKNLKTTTI ESAKFLHDGG WDYSKRYFMV
360 370 380 390 400
AANASNKVAA VDTKTGKLAA LIDTAKIPHP GRGANFVHPQ FGPVWSTGHL
410 420 430 440 450
GDDVVSLIST PSEESKYAKY KEHNWKVVQE LKMPGAGNLF VKTHPKSKHF
460 470 480 490 500
WADAPMNPER EVAESVYVFD MNDLSKAPIQ LNVAKDSGLP ESKAIRRAVQ
510 520 530 540 550
PEYNKAGDEV WISLWGGKTD QSAIVIYDDK TLKLKRVITD PAVVTPTGKF
560
NVFNTMNDVY
Length:560
Mass (Da):61,993
Last modified:March 1, 1992 - v1
Checksum:i476259C35FBFA7A0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X53676 Genomic DNA. Translation: CAA40150.1.
PIRiS13613. OSPSZ.

Genome annotation databases

KEGGiag:CAA40150.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X53676 Genomic DNA. Translation: CAA40150.1.
PIRiS13613. OSPSZ.

3D structure databases

ProteinModelPortaliP24040.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

KEGGiag:CAA40150.

Phylogenomic databases

KOiK15864.

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-201.
BRENDAi1.7.2.1. 5158.

Family and domain databases

Gene3Di1.10.760.10. 1 hit.
2.130.10.10. 1 hit.
2.140.10.20. 1 hit.
InterProiIPR009056. Cyt_c-like_dom.
IPR003143. Cyt_cd1_C.
IPR011048. Haem_d1.
IPR015943. WD40/YVTN_repeat-like_dom.
[Graphical view]
PfamiPF13442. Cytochrome_CBB3. 1 hit.
[Graphical view]
SUPFAMiSSF46626. SSF46626. 1 hit.
SSF51004. SSF51004. 1 hit.
PROSITEiPS51007. CYTC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNIRS_PSEST
AccessioniPrimary (citable) accession number: P24040
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: March 1, 1992
Last modified: October 5, 2016
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.