Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Denitrification system component NirT

Gene

nirT

Organism
Pseudomonas stutzeri (Pseudomonas perfectomarina)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei58 – 581Heme 1 (covalent)By similarity
Binding sitei61 – 611Heme 1 (covalent)By similarity
Metal bindingi62 – 621Iron (heme 1 axial ligand)By similarity
Binding sitei88 – 881Heme 2 (covalent)By similarity
Binding sitei91 – 911Heme 2 (covalent)By similarity
Metal bindingi92 – 921Iron (heme 2 axial ligand)By similarity
Binding sitei148 – 1481Heme 3 (covalent)By similarity
Binding sitei151 – 1511Heme 3 (covalent)By similarity
Metal bindingi152 – 1521Iron (heme 3 axial ligand)By similarity
Binding sitei180 – 1801Heme 4 (covalent)By similarity
Binding sitei183 – 1831Heme 4 (covalent)By similarity
Metal bindingi184 – 1841Iron (heme 4 axial ligand)By similarity

GO - Molecular functioni

  1. heme binding Source: InterPro
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. denitrification pathway Source: InterPro
  2. oxidation-reduction process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Electron transport, Transport

Keywords - Ligandi

Heme, Iron, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Denitrification system component NirT
Gene namesi
Name:nirT
OrganismiPseudomonas stutzeri (Pseudomonas perfectomarina)
Taxonomic identifieri316 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 2424CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei25 – 4521HelicalSequence AnalysisAdd
BLAST
Topological domaini46 – 201156PeriplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 201201Denitrification system component NirTPRO_0000108438Add
BLAST

Post-translational modificationi

Binds 4 heme groups per subunit.

Expressioni

Inductioni

By anaerobic conditions.

Structurei

3D structure databases

ProteinModelPortaliP24038.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Keywords - Domaini

Transmembrane, Transmembrane helix

Family and domain databases

InterProiIPR011031. Multihaem_cyt.
IPR024717. NapC/NirT/NrfH.
IPR005126. NapC/NirT_cyt_c_N.
IPR011885. NO3Rdtase_cyt_c_NapC/NirT.
[Graphical view]
PfamiPF03264. Cytochrom_NNT. 1 hit.
[Graphical view]
PIRSFiPIRSF000013. 4_hem_cytochrm_NapC. 1 hit.
TIGRFAMsiTIGR02161. napC_nirT. 1 hit.
PROSITEiPS51008. MULTIHEME_CYTC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P24038-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDKDGNKQQ KGGILALLRR PSTRYSLGGI LIVGIVAGIV FWGGFNTALE
60 70 80 90 100
ATNTETFCIS CHEMGDNVYP EYKETIHYAN RTGVRATCPD CHVPRDWTHK
110 120 130 140 150
MVRKVEASKE LWGKIVGTID TAEKFEAKRL TLARREWARM RASDSRECRN
160 170 180 190 200
CHSLESMSSD MQKQRARKQH EMAREDNLTC IACHKGIAHH LPEGMTEEDE

D
Length:201
Mass (Da):22,825
Last modified:March 1, 1992 - v1
Checksum:i68B20780C422A450
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X53676 Genomic DNA. Translation: CAA40151.1.
PIRiS13937. O4PSZ.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X53676 Genomic DNA. Translation: CAA40151.1.
PIRiS13937. O4PSZ.

3D structure databases

ProteinModelPortaliP24038.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

InterProiIPR011031. Multihaem_cyt.
IPR024717. NapC/NirT/NrfH.
IPR005126. NapC/NirT_cyt_c_N.
IPR011885. NO3Rdtase_cyt_c_NapC/NirT.
[Graphical view]
PfamiPF03264. Cytochrom_NNT. 1 hit.
[Graphical view]
PIRSFiPIRSF000013. 4_hem_cytochrm_NapC. 1 hit.
TIGRFAMsiTIGR02161. napC_nirT. 1 hit.
PROSITEiPS51008. MULTIHEME_CYTC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The nirSTBM region coding for cytochrome cd1-dependent nitrite respiration of Pseudomonas stutzeri consists of a cluster of mono-, di-, and tetraheme proteins."
    Juengst A., Wakabayashi S., Matsubara H., Zumft W.G.
    FEBS Lett. 279:205-209(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 14405 / 218 / ZoBell.

Entry informationi

Entry nameiNIRT_PSEST
AccessioniPrimary (citable) accession number: P24038
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: March 1, 1992
Last modified: January 7, 2015
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.