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P24033

- CDK1B_XENLA

UniProt

P24033 - CDK1B_XENLA

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Protein
Cyclin-dependent kinase 1-B
Gene
cdk1-b, cdc2, cdc2x1.2
Organism
Xenopus laevis (African clawed frog)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Plays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. Component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II By similarity.

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.
ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.

Enzyme regulationi

Phosphorylation at Thr-14 or Tyr-15 inactivates the enzyme, while phosphorylation at Thr-161 activates it.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei33 – 331ATP By similarity
Active sitei128 – 1281Proton acceptor By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi10 – 189ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. RNA polymerase II carboxy-terminal domain kinase activity Source: UniProtKB-EC
  3. cyclin-dependent protein serine/threonine kinase activity Source: UniProtKB
  4. protein binding Source: UniProtKB
  5. protein serine/threonine kinase activity Source: UniProtKB

GO - Biological processi

  1. mitotic nuclear division Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.22. 6726.

Names & Taxonomyi

Protein namesi
Recommended name:
Cyclin-dependent kinase 1-B (EC:2.7.11.22, EC:2.7.11.23)
Short name:
CDK1-B
Alternative name(s):
Cell division control protein 2 homolog 2
Cell division control protein 2-B
Cell division protein kinase 1
p34 protein kinase 2
Gene namesi
Name:cdk1-b
Synonyms:cdc2, cdc2x1.2
OrganismiXenopus laevis (African clawed frog)
Taxonomic identifieri8355 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Organism-specific databases

XenbaseiXB-GENE-6254942. cdk1.

Subcellular locationi

Nucleus By similarity

GO - Cellular componenti

  1. cytosol Source: Reactome
  2. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 302302Cyclin-dependent kinase 1-B
PRO_0000085736Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei14 – 141Phosphothreonine By similarity
Modified residuei15 – 151Phosphotyrosine; by wee1 and wee22 Publications
Modified residuei161 – 1611Phosphothreonine; by cak1 Publication
Modified residuei277 – 2771Phosphoserine By similarity

Post-translational modificationi

Phosphorylation at Tyr-15 by wee1 and wee2 inhibits the protein kinase activity and acts negative regulator of entry into mitosis (G2 to M transition).

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiP24033.

Interactioni

Subunit structurei

Forms a stable but non-covalent complex with a regulatory subunit and with a cyclin. Interacts with spdya.3 Publications

Structurei

3D structure databases

ProteinModelPortaliP24033.
SMRiP24033. Positions 1-292.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini4 – 287284Protein kinase
Add
BLAST

Sequence similaritiesi

Phylogenomic databases

HOVERGENiHBG014652.
KOiK02087.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P24033-1 [UniParc]FASTAAdd to Basket

« Hide

MDEYTKIEKI GEGTYGVVYK GRHKATGQVV AMKKIRLENE EEGVPSTAIR    50
EISLLKELQH PNIVCLLDVL MQDSRLYLIF EFLSMDLKKY LDSIPSGQYI 100
DTMLVKSYLY QILQGIVFCH SRRVLHRDLK PQNLLIDNKG VIKLADFGLA 150
RAFGIPVRVY THEVVTLWYR ASEVLLGSVR YSTPVDVWSV GTIFAEIATK 200
KPLFHGDSEI DQLFRIFRSL GTPNNEVWPE VESLQDYKNT FPKWKGGSLS 250
SNVKNIDEDG LDLLSKMLVY DPAKRISARK AMLHPYFDDL DKSSLPANQI 300
RN 302
Length:302
Mass (Da):34,532
Last modified:May 30, 2006 - v2
Checksum:iFA9DC156392D7CBC
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti87 – 871L → V in AAA63562. 1 Publication
Sequence conflicti123 – 1231R → G in AAA63562. 1 Publication
Sequence conflicti172 – 1721S → P in AAA63562. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M60681 mRNA. Translation: AAA63562.1.
BC054146 mRNA. Translation: AAH54146.1.
PIRiB44349.
RefSeqiNP_001080093.1. NM_001086624.1.
UniGeneiXl.3815.

Genome annotation databases

GeneIDi379785.
KEGGixla:379785.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M60681 mRNA. Translation: AAA63562.1 .
BC054146 mRNA. Translation: AAH54146.1 .
PIRi B44349.
RefSeqi NP_001080093.1. NM_001086624.1.
UniGenei Xl.3815.

3D structure databases

ProteinModelPortali P24033.
SMRi P24033. Positions 1-292.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PRIDEi P24033.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 379785.
KEGGi xla:379785.

Organism-specific databases

CTDi 379785.
Xenbasei XB-GENE-6254942. cdk1.

Phylogenomic databases

HOVERGENi HBG014652.
KOi K02087.

Enzyme and pathway databases

BRENDAi 2.7.11.22. 6726.

Family and domain databases

InterProi IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
Pfami PF00069. Pkinase. 1 hit.
[Graphical view ]
SMARTi SM00220. S_TKc. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Requirement of mosXe protein kinase for meiotic maturation of Xenopus oocytes induced by a cdc2 mutant lacking regulatory phosphorylation sites."
    Pickham K.M., Meyer A.N., Li J., Donoghue D.J.
    Mol. Cell. Biol. 12:3192-3203(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], ENZYME REGULATION, SUBUNIT.
    Tissue: Oocyte.
  2. NIH - Xenopus Gene Collection (XGC) project
    Submitted (JUN-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Tadpole.
  3. "The MO15 gene encodes the catalytic subunit of a protein kinase that activates cdc2 and other cyclin-dependent kinases (CDKs) through phosphorylation of Thr161 and its homologues."
    Fesquet D., Labbe J.-C., Derancourt J., Capony J.-P., Galas S., Girard F., Lorca T., Shuttleworth J., Doree M., Cavadore J.-C.
    EMBO J. 12:3111-3121(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION AT THR-161.
  4. "Cell cycle regulation of a Xenopus Wee1-like kinase."
    Mueller P.R., Coleman T.R., Dunphy W.G.
    Mol. Biol. Cell 6:119-134(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION AT TYR-15.
  5. "Analysis of the early embryonic cell cycles of Xenopus; regulation of cell cycle length by Xe-wee1 and Mos."
    Murakami M.S., Vande Woude G.F.
    Development 125:237-248(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION AT TYR-15.
  6. "A novel p34cdc2 binding and activating protein that is necessary and sufficient to trigger G2/M progression in Xenopus oocytes."
    Ferby I., Blazquez M., Palmer A., Eritja R., Nebreda A.R.
    Genes Dev. 13:2177-2189(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SPDYA.
  7. "Identification and comparative analysis of multiple mammalian Speedy/Ringo proteins."
    Cheng A., Xiong W., Ferrell J.E. Jr., Solomon M.J.
    Cell Cycle 4:155-165(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SPDYA.

Entry informationi

Entry nameiCDK1B_XENLA
AccessioniPrimary (citable) accession number: P24033
Secondary accession number(s): Q7SZ44
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: May 30, 2006
Last modified: September 3, 2014
This is version 104 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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