Reviewed, UniProtKB/Swiss-Prot P24033 (CDC2B_XENLA)

Names and origin

Protein names

Recommended name:
    Cell division control protein 2-B
    EC=2.7.11.22
    EC=2.7.11.23
Alternative name(s):
    Cell division control protein 2 homolog 2
    p34 protein kinase 2

Gene names

Name:	cdc2-B
Synonyms:	cdc2x1.2

Organism

Xenopus laevis (African clawed frog)

Taxonomic identifier

8355 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Amphibia › Batrachia › Anura › Mesobatrachia › Pipoidea › Pipidae › Xenopodinae › Xenopus › Xenopus

Protein attributes

Sequence length	302 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Plays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. Component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II By similarity.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein. ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.
Enzyme regulation	Phosphorylation at Thr-14 or Tyr-15 inactivates the enzyme, while phosphorylation at Thr-161 activates it. Ref.1
Subunit structure	Forms a stable but non-covalent complex with a regulatory subunit and with a cyclin. Interacts with spdya. Ref.1 Ref.4 Ref.5
Subcellular location	Nucleus By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Biological process	Cell cycle Cell division Mitosis
Cellular component	Nucleus
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	cell division Inferred from electronic annotation. Source: UniProtKB-KW mitosis Inferred from electronic annotation. Source: UniProtKB-KW protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA polymerase subunit kinase activity Inferred from electronic annotation. Source: EC cyclin-dependent protein kinase activity Inferred from electronic annotation. Source: EC protein binding Ref.4 Ref.5 Inferred from physical interaction. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 302	302	Cell division control protein 2-B		PRO_0000085736
Regions
	Domain	4 – 287	284	Protein kinase
	Nucleotide binding	10 – 18	9	ATP By similarity
Sites
	Active site	128	1	Proton acceptor By similarity
	Binding site	33	1	ATP By similarity
Amino acid modifications
	Modified residue	14	1	Phosphothreonine By similarity
	Modified residue	15	1	Phosphotyrosine By similarity
	Modified residue	161	1	Phosphothreonine; by CAK Ref.3
	Modified residue	277	1	Phosphoserine By similarity
Experimental info
	Sequence conflict	87	1	L → V in AAA63562. Ref.1
	Sequence conflict	123	1	R → G in AAA63562. Ref.1
	Sequence conflict	172	1	S → P in AAA63562. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P24033-1 [UniParc]. Last modified May 30, 2006. Version 2. Checksum: FA9DC156392D7CBC	FASTA	302	34,532
	10 20 30 40 50 60 MDEYTKIEKI GEGTYGVVYK GRHKATGQVV AMKKIRLENE EEGVPSTAIR EISLLKELQH 70 80 90 100 110 120 PNIVCLLDVL MQDSRLYLIF EFLSMDLKKY LDSIPSGQYI DTMLVKSYLY QILQGIVFCH 130 140 150 160 170 180 SRRVLHRDLK PQNLLIDNKG VIKLADFGLA RAFGIPVRVY THEVVTLWYR ASEVLLGSVR 190 200 210 220 230 240 YSTPVDVWSV GTIFAEIATK KPLFHGDSEI DQLFRIFRSL GTPNNEVWPE VESLQDYKNT 250 260 270 280 290 300 FPKWKGGSLS SNVKNIDEDG LDLLSKMLVY DPAKRISARK AMLHPYFDDL DKSSLPANQI RN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Requirement of mosXe protein kinase for meiotic maturation of Xenopus oocytes induced by a cdc2 mutant lacking regulatory phosphorylation sites." Pickham K.M., Meyer A.N., Li J., Donoghue D.J. Mol. Cell. Biol. 12:3192-3203(1992) [PubMed: 1377775] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], ENZYME REGULATION, SUBUNIT. Tissue: Oocyte.
[2]	NIH - Xenopus Gene Collection (XGC) project Submitted (JUN-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Tadpole.
[3]	"The MO15 gene encodes the catalytic subunit of a protein kinase that activates cdc2 and other cyclin-dependent kinases (CDKs) through phosphorylation of Thr161 and its homologues." Fesquet D., Labbe J.-C., Derancourt J., Capony J.-P., Galas S., Girard F., Lorca T., Shuttleworth J., Doree M., Cavadore J.-C. EMBO J. 12:3111-3121(1993) [PubMed: 8344251] [Abstract] Cited for: PHOSPHORYLATION AT THR-161.
[4]	"A novel p34cdc2 binding and activating protein that is necessary and sufficient to trigger G2/M progression in Xenopus oocytes." Ferby I., Blazquez M., Palmer A., Eritja R., Nebreda A.R. Genes Dev. 13:2177-2189(1999) [PubMed: 10465793] [Abstract] Cited for: INTERACTION WITH SPDYA.
[5]	"Identification and comparative analysis of multiple mammalian Speedy/Ringo proteins." Cheng A., Xiong W., Ferrell J.E. Jr., Solomon M.J. Cell Cycle 4:155-165(2005) [PubMed: 15611625] [Abstract] Cited for: INTERACTION WITH SPDYA.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M60681 mRNA. Translation: AAA63562.1. BC054146 mRNA. Translation: AAH54146.1.
PIR	B44349.
RefSeq	NP_001080093.1.
UniGene	Xl.3815
3D structure databases
HSSP	HSSP built from PDB template 1P2A based on UniProtKB P24941.
ModBase	Search...
Genome annotation databases
GeneID	379785.
KEGG	xla:379785.
Phylogenomic databases
HOVERGEN	P24033.
Enzyme and pathway databases
BRENDA	2.7.11.22. 648. 2.7.11.23. 648.
Family and domain databases
InterPro	IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_pkinase-rel. IPR008271. Ser_thr_pkin_AS. IPR002290. Ser_thr_pkinase. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
ProDom	PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CDC2B_XENLA

Accession

Primary (citable) accession number: P24033
Secondary accession number(s): Q7SZ44

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 1992
Last sequence update:	May 30, 2006
Last modified:	June 16, 2009
This is version 73 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Xenopus annotation project