Reviewed, UniProtKB/Swiss-Prot P24031 (PPA3_YEAST)

Names and origin

Protein names

Recommended name:
    Constitutive acid phosphatase
    EC=3.1.3.2

Gene names

Name:	PHO3
Ordered Locus Names:	YBR092C
ORF Names:	YBR0813

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	467 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	A phosphate monoester + H2O = an alcohol + phosphate.
Miscellaneous	Present with 952 molecules/cell in log phase SD medium. Ref.4
Sequence similarities	Belongs to the histidine acid phosphatase family.

Ontologies

Keywords
Domain	Signal
Molecular function	Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phosphate metabolic process Inferred from direct assay. Source: SGD thiamin and derivative metabolic process Inferred from direct assay. Source: SGD
Cellular component	cell wall-bounded periplasmic space Inferred from mutant phenotype. Source: SGD
Molecular function	acid phosphatase activity Inferred from mutant phenotype. Source: SGD identical protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
itself		1	EBI-13728,EBI-13728

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 17	17	Potential
	Chain	18 – 467	450	Constitutive acid phosphatase		PRO_0000023953
Sites
	Active site	75	1	Nucleophile By similarity
	Active site	338	1	Proton donor By similarity
Amino acid modifications
	Glycosylation	97	1	N-linked (GlcNAc...) Potential
	Glycosylation	103	1	N-linked (GlcNAc...) Potential
	Glycosylation	162	1	N-linked (GlcNAc...) Potential
	Glycosylation	192	1	N-linked (GlcNAc...) Potential
	Glycosylation	250	1	N-linked (GlcNAc...) Potential
	Glycosylation	315	1	N-linked (GlcNAc...) Potential
	Glycosylation	356	1	N-linked (GlcNAc...) Potential
	Glycosylation	390	1	N-linked (GlcNAc...) Potential
	Glycosylation	439	1	N-linked (GlcNAc...) Potential
	Glycosylation	445	1	N-linked (GlcNAc...) Potential
	Glycosylation	456	1	N-linked (GlcNAc...) Potential
	Glycosylation	461	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	219 – 221	3	DED → MKT in CAA25557. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P24031-1 [UniParc]. Last modified October 1, 1994. Version 2. Checksum: 05FBB80DEB41B0FF	FASTA	467	52,777
	10 20 30 40 50 60 MFKSVVYSVL AAALVNAGTI PLGELADVAK IGTQEDIFPF LGGAGPYFSF PGDYGISRDL 70 80 90 100 110 120 PEGCEMKQLQ MLARHGERYP TYSKGATIMK TWYKLSNYTR QFNGSLSFLN DDYEFFIRDD 130 140 150 160 170 180 DDLEMETTFA NSDNVLNPYT GEMDAKRHAR EFLAQYGYMF ENQTSFPIFA ASSERVHDTA 190 200 210 220 230 240 QYFIDGLGDQ FNISLQTVSE AMSAGANTLS AGNACPGWDE DANDDILDKY DTTYLDDIAK 250 260 270 280 290 300 RLNKENKGLN LTSKDANTLF AWCAYELNAR GYSDVCDIFT EDELVRYSYG QDLVSFYQDG 310 320 330 340 350 360 PGYDMIRSVG ANLFNATLKL LKQSETQDLK VWLSFTHDTD ILNYLTTAGI IDDKNNLTAE 370 380 390 400 410 420 YVPFMGNTFH KSWYVPQGAR VYTEKFQCSN DTYVRYVIND AVVPIETCST GPGFSCEIND 430 440 450 460 FYDYAEKRVA GTDFLKVCNV SSVSNVTELT FYWDWNTTHY NDTLLKQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structural analysis of the two tandemly repeated acid phosphatase genes in yeast." Bajwa W., Meyhack B., Rudolph H., Schweingruber A.-M., Hinnen A. Nucleic Acids Res. 12:7721-7739(1984) [PubMed: 6093051] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Analysis of a 70 kb region on the right arm of yeast chromosome II." Mannhaupt G., Stucka R., Ehnle S., Vetter I., Feldmann H. Yeast 10:1363-1381(1994) [PubMed: 7900426] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"Complete DNA sequence of yeast chromosome II." Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K. EMBO J. 13:5795-5809(1994) [PubMed: 7813418] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X01080 Genomic DNA. Translation: CAA25557.1. X78993 Genomic DNA. Translation: CAA55597.1. Z35961 Genomic DNA. Translation: CAA85045.1.
PIR	PABYCC. S48259.
RefSeq	NP_009650.1.
3D structure databases
HSSP	HSSP built from PDB template 1QFX based on UniProtKB P34755.
ModBase	Search...
Protein-protein interaction databases
IntAct	P24031. 1 interaction.
Proteomic databases
PeptideAtlas	P24031.
Genome annotation databases
Ensembl	YBR092C. Saccharomyces cerevisiae. [Contig view]
GeneID	852389.
GenomeReviews	Gene locus YBR092C in contig Y13134_GR.
KEGG	sce:YBR092C.
NMPDR	fig|4932.3.peg.349.
Organism-specific databases
CYGD	YBR092c.
SGD	S000000296. PHO3.
Yeast-GFP	Search...
Phylogenomic databases
HOGENOM	P24031.
OMA	P24031. WDEDAND.
Enzyme and pathway databases
BRENDA	3.1.3.2. 250.
Gene expression databases
GermOnline	YBR092C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR000560. Histidine_acid_Pase. IPR016274. Histidine_acid_Pase_euk. [Graphical view]
Pfam	PF00328. Acid_phosphat_A. 1 hit. [Graphical view]
PIRSF	PIRSF000894. Acid_phosphatase. 1 hit.
PROSITE	PS00616. HIS_ACID_PHOSPHAT_1. 1 hit. PS00778. HIS_ACID_PHOSPHAT_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	971204.

Entry information

Entry name

PPA3_YEAST

Accession

Primary (citable) accession number: P24031

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 1992
Last sequence update:	October 1, 1994
Last modified:	June 16, 2009
This is version 78 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)