Constitutive acid phosphatase precursor - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P24031 (PPA3_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 111. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Constitutive acid phosphatase
EC=3.1.3.2

Gene names

Name:	PHO3
Ordered Locus Names:	YBR092C
ORF Names:	YBR0813

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	467 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	A phosphate monoester + H₂O = an alcohol + phosphate.
Miscellaneous	Present with 952 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the histidine acid phosphatase family.

Ontologies

Keywords
Domain	Signal
Molecular function	Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	thiamine-containing compound metabolic process Inferred from direct assay PubMed 2407294. Source: SGD
Cellular_component	cell wall-bounded periplasmic space Inferred from mutant phenotype PubMed 2676709. Source: SGD
Molecular_function	acid phosphatase activity Inferred from mutant phenotype PubMed 2676709. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

17

Potential

Chain

450

Constitutive acid phosphatase

PRO_0000023953

Sites

Active site

1

Nucleophile By similarity

Active site

1

Proton donor By similarity

Amino acid modifications

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict

3

DED → MKT in CAA25557. Ref.1

Sequences

Sequence

Length

Mass (Da)

Tools

P24031 [UniParc].

Last modified October 1, 1994. Version 2.
Checksum: 05FBB80DEB41B0FF
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467

52,777

        10         20         30         40         50         60 
MFKSVVYSVL AAALVNAGTI PLGELADVAK IGTQEDIFPF LGGAGPYFSF PGDYGISRDL 

        70         80         90        100        110        120 
PEGCEMKQLQ MLARHGERYP TYSKGATIMK TWYKLSNYTR QFNGSLSFLN DDYEFFIRDD 

       130        140        150        160        170        180 
DDLEMETTFA NSDNVLNPYT GEMDAKRHAR EFLAQYGYMF ENQTSFPIFA ASSERVHDTA 

       190        200        210        220        230        240 
QYFIDGLGDQ FNISLQTVSE AMSAGANTLS AGNACPGWDE DANDDILDKY DTTYLDDIAK 

       250        260        270        280        290        300 
RLNKENKGLN LTSKDANTLF AWCAYELNAR GYSDVCDIFT EDELVRYSYG QDLVSFYQDG 

       310        320        330        340        350        360 
PGYDMIRSVG ANLFNATLKL LKQSETQDLK VWLSFTHDTD ILNYLTTAGI IDDKNNLTAE 

       370        380        390        400        410        420 
YVPFMGNTFH KSWYVPQGAR VYTEKFQCSN DTYVRYVIND AVVPIETCST GPGFSCEIND 

       430        440        450        460 
FYDYAEKRVA GTDFLKVCNV SSVSNVTELT FYWDWNTTHY NDTLLKQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structural analysis of the two tandemly repeated acid phosphatase genes in yeast." Bajwa W., Meyhack B., Rudolph H., Schweingruber A.-M., Hinnen A. Nucleic Acids Res. 12:7721-7739(1984) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Analysis of a 70 kb region on the right arm of yeast chromosome II." Mannhaupt G., Stucka R., Ehnle S., Vetter I., Feldmann H. Yeast 10:1363-1381(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"Complete DNA sequence of yeast chromosome II." Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. Kleine K. EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X01080 Genomic DNA. Translation: CAA25557.1. X78993 Genomic DNA. Translation: CAA55597.1. Z35961 Genomic DNA. Translation: CAA85045.1. BK006936 Genomic DNA. Translation: DAA07213.1.
PIR	PABYCC. S48259.
RefSeq	NP_009650.1. NM_001178440.1.
3D structure databases
ProteinModelPortal	P24031.
SMR	P24031. Positions 37-458.
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-664223.
STRING	4932.YBR092C.
Proteomic databases
PaxDb	P24031.
PeptideAtlas	P24031.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YBR092C; YBR092C; YBR092C.
GeneID	852389.
KEGG	sce:YBR092C.
Organism-specific databases
CYGD	YBR092c.
SGD	S000000296. PHO3.
Phylogenomic databases
eggNOG	NOG260296.
GeneTree	ENSGT00510000050349.
HOGENOM	HOG000189191.
KO	K01078.
OMA	WDEDAND.
OrthoDB	EOG45HW5S.
Gene expression databases
Genevestigator	P24031.
GermOnline	YBR092C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR000560. His_Pase_superF_clade-2. IPR016274. Histidine_acid_Pase_euk. [Graphical view]
Pfam	PF00328. His_Phos_2. 1 hit. [Graphical view]
PIRSF	PIRSF000894. Acid_phosphatase. 1 hit.
PROSITE	PS00616. HIS_ACID_PHOSPHAT_1. 1 hit. PS00778. HIS_ACID_PHOSPHAT_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	971204.

Entry information

Entry name

PPA3_YEAST

Accession

Primary (citable) accession number: P24031
Secondary accession number(s): D6VQ93

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 1992
Last sequence update:	October 1, 1994
Last modified:	May 1, 2013
This is version 111 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome II

Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

SIMILARITY comments

Index of protein domains and families

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents