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Protein

Constitutive acid phosphatase

Gene

PHO3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

A phosphate monoester + H2O = an alcohol + phosphate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei75NucleophileBy similarity1
Active sitei338Proton donorBy similarity1

GO - Molecular functioni

  • acid phosphatase activity Source: SGD

GO - Biological processi

  • phosphate-containing compound metabolic process Source: SGD
  • thiamine-containing compound metabolic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Enzyme and pathway databases

BioCyciYEAST:YBR092C-MONOMER.
BRENDAi3.1.3.2. 984.

Names & Taxonomyi

Protein namesi
Recommended name:
Constitutive acid phosphatase (EC:3.1.3.2)
Gene namesi
Name:PHO3
Ordered Locus Names:YBR092C
ORF Names:YBR0813
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:YBR092C.
SGDiS000000296. PHO3.

Subcellular locationi

GO - Cellular componenti

  • cell wall-bounded periplasmic space Source: SGD
  • fungal-type cell wall Source: GO_Central
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 17Sequence analysisAdd BLAST17
ChainiPRO_000002395318 – 467Constitutive acid phosphataseAdd BLAST450

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi97N-linked (GlcNAc...)Sequence analysis1
Glycosylationi103N-linked (GlcNAc...)Sequence analysis1
Glycosylationi162N-linked (GlcNAc...)Sequence analysis1
Glycosylationi192N-linked (GlcNAc...)Sequence analysis1
Glycosylationi250N-linked (GlcNAc...)Sequence analysis1
Glycosylationi315N-linked (GlcNAc...)Sequence analysis1
Glycosylationi356N-linked (GlcNAc...)Sequence analysis1
Glycosylationi390N-linked (GlcNAc...)Sequence analysis1
Glycosylationi439N-linked (GlcNAc...)Sequence analysis1
Glycosylationi445N-linked (GlcNAc...)Sequence analysis1
Glycosylationi456N-linked (GlcNAc...)Sequence analysis1
Glycosylationi461N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiP24031.
PRIDEiP24031.

PTM databases

iPTMnetiP24031.

Interactioni

Protein-protein interaction databases

BioGridi32798. 33 interactors.
MINTiMINT-664223.

Structurei

3D structure databases

ProteinModelPortaliP24031.
SMRiP24031.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the histidine acid phosphatase family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

HOGENOMiHOG000189191.
InParanoidiP24031.
KOiK01078.
OMAiSHIATIR.
OrthoDBiEOG092C2L5B.

Family and domain databases

CDDicd07061. HP_HAP_like. 1 hit.
Gene3Di3.40.50.1240. 1 hit.
InterProiIPR033379. Acid_Pase_AS.
IPR000560. His_Pase_clade-2.
IPR029033. His_PPase_superfam.
IPR016274. Histidine_acid_Pase_euk.
[Graphical view]
PfamiPF00328. His_Phos_2. 1 hit.
[Graphical view]
PIRSFiPIRSF000894. Acid_phosphatase. 1 hit.
SUPFAMiSSF53254. SSF53254. 1 hit.
PROSITEiPS00616. HIS_ACID_PHOSPHAT_1. 1 hit.
PS00778. HIS_ACID_PHOSPHAT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P24031-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFKSVVYSVL AAALVNAGTI PLGELADVAK IGTQEDIFPF LGGAGPYFSF
60 70 80 90 100
PGDYGISRDL PEGCEMKQLQ MLARHGERYP TYSKGATIMK TWYKLSNYTR
110 120 130 140 150
QFNGSLSFLN DDYEFFIRDD DDLEMETTFA NSDNVLNPYT GEMDAKRHAR
160 170 180 190 200
EFLAQYGYMF ENQTSFPIFA ASSERVHDTA QYFIDGLGDQ FNISLQTVSE
210 220 230 240 250
AMSAGANTLS AGNACPGWDE DANDDILDKY DTTYLDDIAK RLNKENKGLN
260 270 280 290 300
LTSKDANTLF AWCAYELNAR GYSDVCDIFT EDELVRYSYG QDLVSFYQDG
310 320 330 340 350
PGYDMIRSVG ANLFNATLKL LKQSETQDLK VWLSFTHDTD ILNYLTTAGI
360 370 380 390 400
IDDKNNLTAE YVPFMGNTFH KSWYVPQGAR VYTEKFQCSN DTYVRYVIND
410 420 430 440 450
AVVPIETCST GPGFSCEIND FYDYAEKRVA GTDFLKVCNV SSVSNVTELT
460
FYWDWNTTHY NDTLLKQ
Length:467
Mass (Da):52,777
Last modified:October 1, 1994 - v2
Checksum:i05FBB80DEB41B0FF
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti219 – 221DED → MKT in CAA25557 (PubMed:6093051).Curated3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X01080 Genomic DNA. Translation: CAA25557.1.
X78993 Genomic DNA. Translation: CAA55597.1.
Z35961 Genomic DNA. Translation: CAA85045.1.
BK006936 Genomic DNA. Translation: DAA07213.1.
PIRiS48259. PABYCC.
RefSeqiNP_009650.1. NM_001178440.1.

Genome annotation databases

EnsemblFungiiYBR092C; YBR092C; YBR092C.
GeneIDi852389.
KEGGisce:YBR092C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X01080 Genomic DNA. Translation: CAA25557.1.
X78993 Genomic DNA. Translation: CAA55597.1.
Z35961 Genomic DNA. Translation: CAA85045.1.
BK006936 Genomic DNA. Translation: DAA07213.1.
PIRiS48259. PABYCC.
RefSeqiNP_009650.1. NM_001178440.1.

3D structure databases

ProteinModelPortaliP24031.
SMRiP24031.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32798. 33 interactors.
MINTiMINT-664223.

PTM databases

iPTMnetiP24031.

Proteomic databases

MaxQBiP24031.
PRIDEiP24031.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYBR092C; YBR092C; YBR092C.
GeneIDi852389.
KEGGisce:YBR092C.

Organism-specific databases

EuPathDBiFungiDB:YBR092C.
SGDiS000000296. PHO3.

Phylogenomic databases

HOGENOMiHOG000189191.
InParanoidiP24031.
KOiK01078.
OMAiSHIATIR.
OrthoDBiEOG092C2L5B.

Enzyme and pathway databases

BioCyciYEAST:YBR092C-MONOMER.
BRENDAi3.1.3.2. 984.

Miscellaneous databases

PROiP24031.

Family and domain databases

CDDicd07061. HP_HAP_like. 1 hit.
Gene3Di3.40.50.1240. 1 hit.
InterProiIPR033379. Acid_Pase_AS.
IPR000560. His_Pase_clade-2.
IPR029033. His_PPase_superfam.
IPR016274. Histidine_acid_Pase_euk.
[Graphical view]
PfamiPF00328. His_Phos_2. 1 hit.
[Graphical view]
PIRSFiPIRSF000894. Acid_phosphatase. 1 hit.
SUPFAMiSSF53254. SSF53254. 1 hit.
PROSITEiPS00616. HIS_ACID_PHOSPHAT_1. 1 hit.
PS00778. HIS_ACID_PHOSPHAT_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPPA3_YEAST
AccessioniPrimary (citable) accession number: P24031
Secondary accession number(s): D6VQ93
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: October 1, 1994
Last modified: November 30, 2016
This is version 138 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 952 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.