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P24031

- PPA3_YEAST

UniProt

P24031 - PPA3_YEAST

Protein

Constitutive acid phosphatase

Gene

PHO3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 121 (01 Oct 2014)
      Sequence version 2 (01 Oct 1994)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    A phosphate monoester + H2O = an alcohol + phosphate.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei75 – 751NucleophileBy similarity
    Active sitei338 – 3381Proton donorBy similarity

    GO - Molecular functioni

    1. acid phosphatase activity Source: SGD

    GO - Biological processi

    1. dephosphorylation Source: GOC
    2. phosphate-containing compound metabolic process Source: SGD
    3. thiamine-containing compound metabolic process Source: SGD

    Keywords - Molecular functioni

    Hydrolase

    Enzyme and pathway databases

    BioCyciYEAST:YBR092C-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Constitutive acid phosphatase (EC:3.1.3.2)
    Gene namesi
    Name:PHO3
    Ordered Locus Names:YBR092C
    ORF Names:YBR0813
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome II

    Organism-specific databases

    CYGDiYBR092c.
    SGDiS000000296. PHO3.

    Subcellular locationi

    GO - Cellular componenti

    1. cell wall-bounded periplasmic space Source: SGD

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1717Sequence AnalysisAdd
    BLAST
    Chaini18 – 467450Constitutive acid phosphatasePRO_0000023953Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi97 – 971N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi103 – 1031N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi162 – 1621N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi192 – 1921N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi250 – 2501N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi315 – 3151N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi356 – 3561N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi390 – 3901N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi439 – 4391N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi445 – 4451N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi456 – 4561N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi461 – 4611N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    MaxQBiP24031.
    PaxDbiP24031.
    PeptideAtlasiP24031.

    Expressioni

    Gene expression databases

    GenevestigatoriP24031.

    Interactioni

    Protein-protein interaction databases

    BioGridi32798. 33 interactions.
    MINTiMINT-664223.
    STRINGi4932.YBR092C.

    Structurei

    3D structure databases

    ProteinModelPortaliP24031.
    SMRiP24031. Positions 37-458.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the histidine acid phosphatase family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG260296.
    GeneTreeiENSGT00510000050349.
    HOGENOMiHOG000189191.
    KOiK01078.
    OMAiWDEDAND.
    OrthoDBiEOG7TQV9N.

    Family and domain databases

    Gene3Di3.40.50.1240. 1 hit.
    InterProiIPR000560. His_Pase_superF_clade-2.
    IPR029033. His_PPase_superfam.
    IPR016274. Histidine_acid_Pase_euk.
    [Graphical view]
    PfamiPF00328. His_Phos_2. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000894. Acid_phosphatase. 1 hit.
    SUPFAMiSSF53254. SSF53254. 1 hit.
    PROSITEiPS00616. HIS_ACID_PHOSPHAT_1. 1 hit.
    PS00778. HIS_ACID_PHOSPHAT_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P24031-1 [UniParc]FASTAAdd to Basket

    « Hide

    MFKSVVYSVL AAALVNAGTI PLGELADVAK IGTQEDIFPF LGGAGPYFSF    50
    PGDYGISRDL PEGCEMKQLQ MLARHGERYP TYSKGATIMK TWYKLSNYTR 100
    QFNGSLSFLN DDYEFFIRDD DDLEMETTFA NSDNVLNPYT GEMDAKRHAR 150
    EFLAQYGYMF ENQTSFPIFA ASSERVHDTA QYFIDGLGDQ FNISLQTVSE 200
    AMSAGANTLS AGNACPGWDE DANDDILDKY DTTYLDDIAK RLNKENKGLN 250
    LTSKDANTLF AWCAYELNAR GYSDVCDIFT EDELVRYSYG QDLVSFYQDG 300
    PGYDMIRSVG ANLFNATLKL LKQSETQDLK VWLSFTHDTD ILNYLTTAGI 350
    IDDKNNLTAE YVPFMGNTFH KSWYVPQGAR VYTEKFQCSN DTYVRYVIND 400
    AVVPIETCST GPGFSCEIND FYDYAEKRVA GTDFLKVCNV SSVSNVTELT 450
    FYWDWNTTHY NDTLLKQ 467
    Length:467
    Mass (Da):52,777
    Last modified:October 1, 1994 - v2
    Checksum:i05FBB80DEB41B0FF
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti219 – 2213DED → MKT in CAA25557. (PubMed:6093051)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X01080 Genomic DNA. Translation: CAA25557.1.
    X78993 Genomic DNA. Translation: CAA55597.1.
    Z35961 Genomic DNA. Translation: CAA85045.1.
    BK006936 Genomic DNA. Translation: DAA07213.1.
    PIRiS48259. PABYCC.
    RefSeqiNP_009650.1. NM_001178440.1.

    Genome annotation databases

    EnsemblFungiiYBR092C; YBR092C; YBR092C.
    GeneIDi852389.
    KEGGisce:YBR092C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X01080 Genomic DNA. Translation: CAA25557.1 .
    X78993 Genomic DNA. Translation: CAA55597.1 .
    Z35961 Genomic DNA. Translation: CAA85045.1 .
    BK006936 Genomic DNA. Translation: DAA07213.1 .
    PIRi S48259. PABYCC.
    RefSeqi NP_009650.1. NM_001178440.1.

    3D structure databases

    ProteinModelPortali P24031.
    SMRi P24031. Positions 37-458.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 32798. 33 interactions.
    MINTi MINT-664223.
    STRINGi 4932.YBR092C.

    Proteomic databases

    MaxQBi P24031.
    PaxDbi P24031.
    PeptideAtlasi P24031.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YBR092C ; YBR092C ; YBR092C .
    GeneIDi 852389.
    KEGGi sce:YBR092C.

    Organism-specific databases

    CYGDi YBR092c.
    SGDi S000000296. PHO3.

    Phylogenomic databases

    eggNOGi NOG260296.
    GeneTreei ENSGT00510000050349.
    HOGENOMi HOG000189191.
    KOi K01078.
    OMAi WDEDAND.
    OrthoDBi EOG7TQV9N.

    Enzyme and pathway databases

    BioCyci YEAST:YBR092C-MONOMER.

    Miscellaneous databases

    NextBioi 971204.

    Gene expression databases

    Genevestigatori P24031.

    Family and domain databases

    Gene3Di 3.40.50.1240. 1 hit.
    InterProi IPR000560. His_Pase_superF_clade-2.
    IPR029033. His_PPase_superfam.
    IPR016274. Histidine_acid_Pase_euk.
    [Graphical view ]
    Pfami PF00328. His_Phos_2. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000894. Acid_phosphatase. 1 hit.
    SUPFAMi SSF53254. SSF53254. 1 hit.
    PROSITEi PS00616. HIS_ACID_PHOSPHAT_1. 1 hit.
    PS00778. HIS_ACID_PHOSPHAT_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Structural analysis of the two tandemly repeated acid phosphatase genes in yeast."
      Bajwa W., Meyhack B., Rudolph H., Schweingruber A.-M., Hinnen A.
      Nucleic Acids Res. 12:7721-7739(1984) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Analysis of a 70 kb region on the right arm of yeast chromosome II."
      Mannhaupt G., Stucka R., Ehnle S., Vetter I., Feldmann H.
      Yeast 10:1363-1381(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. "Complete DNA sequence of yeast chromosome II."
      Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C.
      , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.
      EMBO J. 13:5795-5809(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiPPA3_YEAST
    AccessioniPrimary (citable) accession number: P24031
    Secondary accession number(s): D6VQ93
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 1, 1992
    Last sequence update: October 1, 1994
    Last modified: October 1, 2014
    This is version 121 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 952 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome II
      Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

    External Data

    Dasty 3