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Protein

Cytochrome c oxidase subunit 1

Gene

ctaD

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Protein inferred from homologyi

Functioni

Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Subunits 1-3 form the functional core of the enzyme complex. Co I is the catalytic subunit of the enzyme. Electrons originating in cytochrome c are transferred via the copper A center of subunit 2 and heme a of subunit 1 to the bimetallic center formed by heme a3 and copper B. This cytochrome c oxidase shows proton pump activity across the membrane in addition to the electron transfer.

Catalytic activityi

4 ferrocytochrome c + O2 + 4 H+ = 4 ferricytochrome c + 2 H2O.

Cofactori

Protein has several cofactor binding sites:
  • Cu2+Note: Binds 1 copper B ion per subunit.
  • hemeNote: Binds 2 heme groups per subunit.

Pathwayi: oxidative phosphorylation

This protein is involved in the pathway oxidative phosphorylation, which is part of Energy metabolism.
View all proteins of this organism that are known to be involved in the pathway oxidative phosphorylation and in Energy metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi73Iron (heme A axial ligand)Curated1
Metal bindingi249Copper BCurated1
Metal bindingi253Copper BCurated1
Metal bindingi298Copper BCurated1
Metal bindingi299Copper BCurated1
Metal bindingi384Iron (heme A3 axial ligand)Curated1
Metal bindingi386Iron (heme A axial ligand)Curated1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Hydrogen ion transport, Ion transport, Respiratory chain, Transport

Keywords - Ligandi

Copper, Heme, Iron, Metal-binding

Enzyme and pathway databases

BioCyciBSUB:BSU14900-MONOMER.
SABIO-RKP24010.
UniPathwayiUPA00705.

Protein family/group databases

TCDBi3.D.4.4.1. the proton-translocating cytochrome oxidase (cox) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome c oxidase subunit 1 (EC:1.9.3.1)
Alternative name(s):
Caa-3605 subunit 1
Cytochrome aa3 subunit 1
Cytochrome c oxidase polypeptide I
Oxidase aa(3) subunit 1
Gene namesi
Name:ctaD
Ordered Locus Names:BSU14900
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 27ExtracellularSequence analysisAdd BLAST27
Transmembranei28 – 46HelicalSequence analysisAdd BLAST19
Topological domaini47 – 68CytoplasmicSequence analysisAdd BLAST22
Transmembranei69 – 88HelicalSequence analysisAdd BLAST20
Topological domaini89 – 110ExtracellularSequence analysisAdd BLAST22
Transmembranei111 – 128HelicalSequence analysisAdd BLAST18
Topological domaini129 – 159CytoplasmicSequence analysisAdd BLAST31
Transmembranei160 – 178HelicalSequence analysisAdd BLAST19
Topological domaini179 – 196ExtracellularSequence analysisAdd BLAST18
Transmembranei197 – 215HelicalSequence analysisAdd BLAST19
Topological domaini216 – 241CytoplasmicSequence analysisAdd BLAST26
Transmembranei242 – 261HelicalSequence analysisAdd BLAST20
Topological domaini262 – 284ExtracellularSequence analysisAdd BLAST23
Transmembranei285 – 304HelicalSequence analysisAdd BLAST20
Topological domaini305 – 312CytoplasmicSequence analysis8
Transmembranei313 – 331HelicalSequence analysisAdd BLAST19
Topological domaini332 – 346ExtracellularSequence analysisAdd BLAST15
Transmembranei347 – 366HelicalSequence analysisAdd BLAST20
Topological domaini367 – 374CytoplasmicSequence analysis8
Transmembranei375 – 394HelicalSequence analysisAdd BLAST20
Topological domaini395 – 421ExtracellularSequence analysisAdd BLAST27
Transmembranei422 – 441HelicalSequence analysisAdd BLAST20
Topological domaini442 – 459CytoplasmicSequence analysisAdd BLAST18
Transmembranei460 – 479HelicalSequence analysisAdd BLAST20
Topological domaini480 – 552ExtracellularSequence analysisAdd BLAST73
Transmembranei553 – 572HelicalSequence analysisAdd BLAST20
Topological domaini573 – 580CytoplasmicSequence analysis8
Transmembranei581 – 604HelicalSequence analysisAdd BLAST24
Topological domaini605 – 622CytoplasmicSequence analysisAdd BLAST18

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001834361 – 622Cytochrome c oxidase subunit 1Add BLAST622

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki249 ↔ 2531'-histidyl-3'-tyrosine (His-Tyr)By similarity

Proteomic databases

PaxDbiP24010.

Interactioni

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100008246.

Structurei

3D structure databases

ProteinModelPortaliP24010.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105BZ9. Bacteria.
COG0843. LUCA.
HOGENOMiHOG000085275.
InParanoidiP24010.
KOiK02274.
OMAiIDDHGFH.
PhylomeDBiP24010.

Family and domain databases

Gene3Di1.20.210.10. 1 hit.
InterProiIPR000883. COX1.
IPR023616. Cyt_c_oxase-like_su1_dom.
IPR023615. Cyt_c_Oxase_su1_BS.
IPR014241. Cyt_c_oxidase_su1_bac.
[Graphical view]
PANTHERiPTHR10422. PTHR10422. 1 hit.
PfamiPF00115. COX1. 1 hit.
[Graphical view]
PRINTSiPR01165. CYCOXIDASEI.
SUPFAMiSSF81442. SSF81442. 1 hit.
TIGRFAMsiTIGR02891. CtaD_CoxA. 1 hit.
PROSITEiPS50855. COX1. 1 hit.
PS00077. COX1_CUB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P24010-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLNALTEKRT RGSMLWDYLT TVDHKKIAIL YLVAGGFFFL VGGIEAMFIR
60 70 80 90 100
IQLAKPENAF LSAQAYNEVM TMHGTTMIFL AAMPLLFALM NAVVPLQIGA
110 120 130 140 150
RDVSFPFLNA LGFWLFFFGG IFLNLSWFLG GAPDAGWTSY ASLSLHSKGH
160 170 180 190 200
GIDFFVLGLQ ISGLGTLIAG INFLATIINM RAPGMTYMRL PLFTWTTFVA
210 220 230 240 250
SALILFAFPP LTVGLALMML DRLFGTNFFN PELGGNTVIW EHLFWIFGHP
260 270 280 290 300
EVYILILPAF GIFSEVIPVF ARKRLFGYSS MVFAIVLIGF LGFMVWVHHM
310 320 330 340 350
FTTGLGPIAN AIFAVATMAI AIPTGIKIFN WLLTIWGGNV KYTTAMLYAV
360 370 380 390 400
SFIPSFVLGG VTGVMLAAAA ADYQFHDTYF VVAHFHYVII GGVVFGLLAG
410 420 430 440 450
VHFWWPKMFG KILHETMGKI SFVLFFIGFH LTFFIQHFVG LMGMPRRVYT
460 470 480 490 500
FLPGQGLETG NLISTIGAFF MAAAVILLLV NVIWTSVKGE YVGADPWHDG
510 520 530 540 550
RTLEWTVSSP PPEYNFKQLP FVRGLDPLWI EKQAGHKSMT PAEPVDDIHM
560 570 580 590 600
PNGSILPLII SFGLFVAAFG LLYRSDYAWG LPVIFIGLGI TFITMLLRSV
610 620
IDDHGYHIHK EELPNDDKGV KA
Length:622
Mass (Da):69,028
Last modified:June 16, 2009 - v3
Checksum:iDF159F21D191332D
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti120G → H in CAB11343 (Ref. 2) Curated1
Sequence conflicti155 – 156FV → SI in CAA38077 (PubMed:1847686).Curated2
Sequence conflicti288Missing in CAA38077 (PubMed:1847686).Curated1
Sequence conflicti474A → R in CAA38077 (PubMed:1847686).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X54140 Genomic DNA. Translation: CAA38077.1.
Z98682 Genomic DNA. Translation: CAB11343.1.
AL009126 Genomic DNA. Translation: CAB13363.2.
PIRiE69609.
RefSeqiNP_389373.2. NC_000964.3.
WP_003232245.1. NZ_JNCM01000035.1.

Genome annotation databases

EnsemblBacteriaiCAB13363; CAB13363; BSU14900.
GeneIDi936898.
KEGGibsu:BSU14900.
PATRICi18974781. VBIBacSub10457_1582.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X54140 Genomic DNA. Translation: CAA38077.1.
Z98682 Genomic DNA. Translation: CAB11343.1.
AL009126 Genomic DNA. Translation: CAB13363.2.
PIRiE69609.
RefSeqiNP_389373.2. NC_000964.3.
WP_003232245.1. NZ_JNCM01000035.1.

3D structure databases

ProteinModelPortaliP24010.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100008246.

Protein family/group databases

TCDBi3.D.4.4.1. the proton-translocating cytochrome oxidase (cox) superfamily.

Proteomic databases

PaxDbiP24010.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB13363; CAB13363; BSU14900.
GeneIDi936898.
KEGGibsu:BSU14900.
PATRICi18974781. VBIBacSub10457_1582.

Phylogenomic databases

eggNOGiENOG4105BZ9. Bacteria.
COG0843. LUCA.
HOGENOMiHOG000085275.
InParanoidiP24010.
KOiK02274.
OMAiIDDHGFH.
PhylomeDBiP24010.

Enzyme and pathway databases

UniPathwayiUPA00705.
BioCyciBSUB:BSU14900-MONOMER.
SABIO-RKP24010.

Family and domain databases

Gene3Di1.20.210.10. 1 hit.
InterProiIPR000883. COX1.
IPR023616. Cyt_c_oxase-like_su1_dom.
IPR023615. Cyt_c_Oxase_su1_BS.
IPR014241. Cyt_c_oxidase_su1_bac.
[Graphical view]
PANTHERiPTHR10422. PTHR10422. 1 hit.
PfamiPF00115. COX1. 1 hit.
[Graphical view]
PRINTSiPR01165. CYCOXIDASEI.
SUPFAMiSSF81442. SSF81442. 1 hit.
TIGRFAMsiTIGR02891. CtaD_CoxA. 1 hit.
PROSITEiPS50855. COX1. 1 hit.
PS00077. COX1_CUB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCOX1_BACSU
AccessioniPrimary (citable) accession number: P24010
Secondary accession number(s): O34467
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: June 16, 2009
Last modified: November 30, 2016
This is version 141 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.