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Reviewed, UniProtKB/Swiss-Prot P24010 (COX1_BACSU)

Last modified June 16, 2009. Version 84. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Cytochrome c oxidase subunit 1
    EC=1.9.3.1
Alternative name(s):
    Cytochrome c oxidase polypeptide I
    Cytochrome aa3 subunit 1
    Caa-3605 subunit 1
    Oxidase aa(3) subunit 1
Gene names
Name: ctaD
Ordered Locus Names: BSU14900
OrganismBacillus subtilis [Complete proteome] [HAMAP]
Taxonomic identifier1423 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

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Protein attributes

Sequence length622 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceInferred from homology.

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General annotation (Comments)

Function

Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Subunits 1-3 form the functional core of the enzyme complex. Co I is the catalytic subunit of the enzyme. Electrons originating in cytochrome c are transferred via the copper A center of subunit 2 and heme a of subunit 1 to the bimetallic center formed by heme a3 and copper B. This cytochrome c oxidase shows proton pump activity across the membrane in addition to the electron transfer.

Catalytic activity

4 ferrocytochrome c + O2 + 4 H+ = 4 ferricytochrome c + 2 H2O.

Cofactor

Binds 1 copper B ion per subunit.

Binds 2 heme groups per subunit.

Pathway

Energy metabolism; oxidative phosphorylation.

Subcellular location

Cell membrane; Multi-pass membrane protein.

Sequence similarities

Belongs to the heme-copper respiratory oxidase family.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 622622Cytochrome c oxidase subunit 1
PRO_0000183436

Regions

Topological domain1 – 2727Extracellular Potential
Transmembrane28 – 4619 Potential
Topological domain47 – 6822Cytoplasmic Potential
Transmembrane69 – 8820 Potential
Topological domain89 – 11022Extracellular Potential
Transmembrane111 – 12818 Potential
Topological domain129 – 15931Cytoplasmic Potential
Transmembrane160 – 17819 Potential
Topological domain179 – 19618Extracellular Potential
Transmembrane197 – 21519 Potential
Topological domain216 – 24126Cytoplasmic Potential
Transmembrane242 – 26120 Potential
Topological domain262 – 28423Extracellular Potential
Transmembrane285 – 30420 Potential
Topological domain305 – 3128Cytoplasmic Potential
Transmembrane313 – 33119 Potential
Topological domain332 – 34615Extracellular Potential
Transmembrane347 – 36620 Potential
Topological domain367 – 3748Cytoplasmic Potential
Transmembrane375 – 39420 Potential
Topological domain395 – 42127Extracellular Potential
Transmembrane422 – 44120 Potential
Topological domain442 – 45918Cytoplasmic Potential
Transmembrane460 – 47920 Potential
Topological domain480 – 55273Extracellular Potential
Transmembrane553 – 57220 Potential
Topological domain573 – 5808Cytoplasmic Potential
Transmembrane581 – 60424 Potential
Topological domain605 – 62218Cytoplasmic Potential

Sites

Metal binding731Iron (heme A axial ligand) Probable
Metal binding2491Copper B Probable
Metal binding2531Copper B Probable
Metal binding2981Copper B Probable
Metal binding2991Copper B Probable
Metal binding3841Iron (heme A3 axial ligand) Probable
Metal binding3861Iron (heme A axial ligand) Probable

Amino acid modifications

Cross-link249 ↔ 2531'-histidyl-3'-tyrosine (His-Tyr) By similarity

Experimental info

Sequence conflict1201G → H in CAB11343. Ref.2
Sequence conflict155 – 1562FV → SI in CAA38077. Ref.1
Sequence conflict2881Missing in CAA38077. Ref.1
Sequence conflict4741A → R in CAA38077. Ref.1

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Sequences

Sequence LengthMass (Da)Tools
P24010-1 [UniParc].

Last modified June 16, 2009. Version 3.
Checksum: DF159F21D191332D

FASTA62269,028
        10         20         30         40         50         60 
MLNALTEKRT RGSMLWDYLT TVDHKKIAIL YLVAGGFFFL VGGIEAMFIR IQLAKPENAF 

        70         80         90        100        110        120 
LSAQAYNEVM TMHGTTMIFL AAMPLLFALM NAVVPLQIGA RDVSFPFLNA LGFWLFFFGG 

       130        140        150        160        170        180 
IFLNLSWFLG GAPDAGWTSY ASLSLHSKGH GIDFFVLGLQ ISGLGTLIAG INFLATIINM 

       190        200        210        220        230        240 
RAPGMTYMRL PLFTWTTFVA SALILFAFPP LTVGLALMML DRLFGTNFFN PELGGNTVIW 

       250        260        270        280        290        300 
EHLFWIFGHP EVYILILPAF GIFSEVIPVF ARKRLFGYSS MVFAIVLIGF LGFMVWVHHM 

       310        320        330        340        350        360 
FTTGLGPIAN AIFAVATMAI AIPTGIKIFN WLLTIWGGNV KYTTAMLYAV SFIPSFVLGG 

       370        380        390        400        410        420 
VTGVMLAAAA ADYQFHDTYF VVAHFHYVII GGVVFGLLAG VHFWWPKMFG KILHETMGKI 

       430        440        450        460        470        480 
SFVLFFIGFH LTFFIQHFVG LMGMPRRVYT FLPGQGLETG NLISTIGAFF MAAAVILLLV 

       490        500        510        520        530        540 
NVIWTSVKGE YVGADPWHDG RTLEWTVSSP PPEYNFKQLP FVRGLDPLWI EKQAGHKSMT 

       550        560        570        580        590        600 
PAEPVDDIHM PNGSILPLII SFGLFVAAFG LLYRSDYAWG LPVIFIGLGI TFITMLLRSV 

       610        620 
IDDHGYHIHK EELPNDDKGV KA

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References

« Hide 'large scale' references
[1]"The Bacillus subtilis cytochrome-c oxidase. Variations on a conserved protein theme."
Saraste M., Metso T., Nakari T., Jalli T., Lauraeus M., van der Oost J.
Eur. J. Biochem. 195:517-525(1991) [PubMed: 1847686] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 168.
[2]"Bacillus subtilis chromosomal region downstream nprE."
Bertero M., Presecan E., Glaser P., Richou A., Danchin A.
Submitted (AUG-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 168.
[3]"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. expand/collapse author list , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
Nature 390:249-256(1997) [PubMed: 9384377] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 168.
[4]"From a consortium sequence to a unified sequence: the Bacillus subtilis 168 reference genome a decade later."
Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A., Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.
Microbiology 0:0-0(2009) [PubMed: 19383706] [Abstract]
Cited for: SEQUENCE REVISION TO 120.

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Cross-references

Sequence databases

X54140 Genomic DNA. Translation: CAA38077.1.
Z98682 Genomic DNA. Translation: CAB11343.1.
AL009126 Genomic DNA. Translation: CAB13363.2.
PIRE69609.
RefSeqNP_389373.1.

3D structure databases

HSSPHSSP built from PDB template 1FFT based on UniProtKB P18401.
ModBaseSearch...

Protein family/group databases

TCDB3.D.4.4.1. proton-translocating cytochrome oxidase (COX) superfamily.

Genome annotation databases

GeneID936898.
GenomeReviewsGene locus BSU14900 in contig AL009126_GR.
KEGGbsu:BSU14900.
NMPDRfig|224308.1.peg.1492.

Organism-specific databases

SubtiListBG10216. ctaD. [Micado]
CMRSearch...

Phylogenomic databases

HOGENOMP24010.
OMAP24010. IFAVATM.

Enzyme and pathway databases

BioCycBSUB224308:BSU1492-MON.
BRENDA1.9.3.1. 150.

Family and domain databases

InterProIPR000883. Cyt_c_oxidase_su1.
IPR014241. Cyt_c_oxidase_su1_bac.
[Graphical view]
Gene3DG3DSA:1.20.210.10. COX1. 1 hit.
PANTHERPTHR10422. COX1. 1 hit.
PfamPF00115. COX1. 1 hit.
[Graphical view]
PRINTSPR01165. CYCOXIDASEI.
TIGRFAMsTIGR02891. CtaD_CoxA. 1 hit.
PROSITEPS50855. COX1. 1 hit.
PS00077. COX1_CUB. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameCOX1_BACSU
AccessionPrimary (citable) accession number: P24010
Secondary accession number(s): O34467
Entry history
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: June 16, 2009
Last modified: June 16, 2009
This is version 84 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

Bacillus subtilis

Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents