P24008 (S5A1_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-oxo-5-alpha-steroid 4-dehydrogenase 1
EC=1.3.99.5

Alternative name(s):
SR type 1
Steroid 5-alpha-reductase 1
Short name=S5AR 1

Gene names

Name:

Srd5a1

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	259 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Converts testosterone into 5-alpha-dihydrotestosterone and progesterone or corticosterone into their corresponding 5-alpha-3-oxosteroids. It plays a central role in sexual differentiation and androgen physiology.
Catalytic activity	A 3-oxo-5-alpha-steroid + acceptor = a 3-oxo-Delta(4)-steroid + reduced acceptor.
Subcellular location	Microsome membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein Potential.
Tissue specificity	Liver and prostate (at a low level).
Developmental stage	After the establishment of chromosomal sex at fertilization.
Induction	Its expression is regulated by androgens such as testosterone.
Sequence similarities	Belongs to the steroid 5-alpha reductase family.
Biophysicochemical properties	pH dependence: Optimally active at alkaline pHs.

Ontologies

Keywords
Biological process	Differentiation Sexual differentiation
Cellular component	Endoplasmic reticulum Membrane Microsome
Coding sequence diversity	Alternative initiation
Domain	Transmembrane Transmembrane helix
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	androgen biosynthetic process Inferred from direct assay. Source: RGD bone development Inferred from expression pattern. Source: RGD cell differentiation Inferred from electronic annotation. Source: UniProtKB-KW cellular response to cAMP Inferred from expression pattern. Source: RGD cellular response to dexamethasone stimulus Inferred from expression pattern. Source: RGD cellular response to epinephrine stimulus Inferred from expression pattern. Source: RGD cellular response to estradiol stimulus Inferred from expression pattern. Source: RGD cellular response to growth factor stimulus Inferred from expression pattern. Source: RGD cellular response to insulin stimulus Inferred from expression pattern. Source: RGD cellular response to organic cyclic compound Inferred from expression pattern. Source: RGD cellular response to starvation Inferred from expression pattern. Source: RGD cellular response to testosterone stimulus Inferred from expression pattern. Source: RGD cerebral cortex development Inferred from expression pattern. Source: RGD circadian sleep/wake cycle, REM sleep Inferred from expression pattern. Source: RGD diterpenoid metabolic process Inferred from expression pattern. Source: RGD female genitalia development Inferred from expression pattern. Source: RGD hippocampus development Inferred from expression pattern. Source: RGD hypothalamus development Inferred from expression pattern. Source: RGD liver development Inferred from expression pattern. Source: RGD male genitalia development Inferred from expression pattern. Source: RGD male gonad development Inferred from expression pattern. Source: RGD pituitary gland development Inferred from expression pattern. Source: RGD progesterone metabolic process Inferred from direct assay. Source: RGD response to drug Inferred from mutant phenotype. Source: RGD response to follicle-stimulating hormone stimulus Inferred from expression pattern. Source: RGD response to fungicide Inferred from expression pattern. Source: RGD response to growth hormone stimulus Inferred from expression pattern. Source: RGD response to muscle activity Inferred from expression pattern. Source: RGD serotonin metabolic process Inferred from expression pattern. Source: RGD spinal cord development Inferred from expression pattern. Source: RGD thalamus development Inferred from expression pattern. Source: RGD urogenital system development Inferred from expression pattern. Source: RGD
Cellular component	cell body fiber Inferred from direct assay. Source: RGD endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW microsome Inferred from direct assay. Source: RGD myelin sheath Inferred from direct assay. Source: RGD neuronal cell body Inferred from direct assay. Source: RGD perinuclear region of cytoplasm Inferred from direct assay. Source: RGD
Molecular function	3-oxo-5-alpha-steroid 4-dehydrogenase activity Inferred from sequence or structural similarity. Source: UniProtKB NADPH binding Inferred from direct assay. Source: RGD amide binding Inferred from direct assay. Source: RGD
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative initiation. [Align] [Select]

Isoform Long (identifier: P24008-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform Short (identifier: P24008-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-4: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 259	259	3-oxo-5-alpha-steroid 4-dehydrogenase 1		PRO_0000031565
Regions
	Transmembrane	10 – 30	21	Helical; Potential
	Transmembrane	86 – 106	21	Helical; Potential
	Transmembrane	111 – 131	21	Helical; Potential
	Transmembrane	146 – 166	21	Helical; Potential
	Transmembrane	206 – 226	21	Helical; Potential
Natural variations
	Alternative sequence	1 – 4	4	Missing in isoform Short.		VSP_018884

Sequences

	Sequence		Length	Mass (Da)
	Isoform Long [UniParc]. Last modified November 1, 1997. Version 2. Checksum: FE6705AC1D7CAE11	FASTA	259	29,780
	10 20 30 40 50 60 MVPLMELDEL CLLDMLVYLE GFMAFVSIVG LRSVGSPYGR YSPQWPGIRV PARPAWFIQE 70 80 90 100 110 120 LPSMAWPLYE YIRPAAARLG NLPNRVLLAM FLIHYVQRTL VFPVLIRGGK PTLLVTFVLA 130 140 150 160 170 180 FLFCTFNGYV QSRYLSQFAV YAEDWVTHPC FLTGFALWLV GMVINIHSDH ILRNLRKPGE 190 200 210 220 230 240 TGYKIPRGGL FEYVSAANYF GELVEWCGFA LASWSLQGVV FALFTLSTLL TRAKQHHQWY 250 HEKFEDYPKS RKILIPFVL
	Isoform Short [UniParc]. Checksum: A8510DF4235CB9CA	FASTA	255	29,339
	10 20 30 40 50 60 MELDELCLLD MLVYLEGFMA FVSIVGLRSV GSPYGRYSPQ WPGIRVPARP AWFIQELPSM 70 80 90 100 110 120 AWPLYEYIRP AAARLGNLPN RVLLAMFLIH YVQRTLVFPV LIRGGKPTLL VTFVLAFLFC 130 140 150 160 170 180 TFNGYVQSRY LSQFAVYAED WVTHPCFLTG FALWLVGMVI NIHSDHILRN LRKPGETGYK 190 200 210 220 230 240 IPRGGLFEYV SAANYFGELV EWCGFALASW SLQGVVFALF TLSTLLTRAK QHHQWYHEKF 250 EDYPKSRKIL IPFVL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Expression cloning and regulation of steroid 5 alpha-reductase, an enzyme essential for male sexual differentiation." Andersson S., Bishop R.W., Russell D.W. J. Biol. Chem. 264:16249-16255(1989) [PubMed: 2476440] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM SHORT).
[2]	"Heterogeneity of rat type I 5 alpha-reductase cDNA: cloning, expression and regulation by pituitary implants and dihydrotestosterone." Lopez-Solache I., Luu-The V., Seralini G.E., Labrie F. Biochim. Biophys. Acta 1305:139-144(1996) [PubMed: 8597599] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM LONG). Tissue: Liver.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM SHORT). Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	J05035 mRNA. Translation: AAA42102.1. S81448 mRNA. Translation: AAB36218.1. BC127454 mRNA. Translation: AAI27455.1.
IPI	IPI00210851. IPI00760114.
PIR	S65744.
RefSeq	NP_058766.2. NM_017070.3.
UniGene	Rn.4620.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	P24008.
Proteomic databases
PRIDE	P24008.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	24950.
KEGG	rno:24950.
UCSC	NM_017070. rat.
Organism-specific databases
CTD	6715.
RGD	3757. Srd5a1.
Phylogenomic databases
eggNOG	roNOG12983.
HOVERGEN	HBG003402.
InParanoid	P24008.
OrthoDB	EOG4VX25X.
Gene expression databases
ArrayExpress	P24008.
Genevestigator	P24008.
GermOnline	ENSRNOG00000017601. Rattus norvegicus.
Family and domain databases
InterPro	IPR016636. 3-oxo-5-alpha-steroid_4-DH. IPR001104. 3-oxo-5_a-steroid_4-DH_C. [Graphical view]
KO	K12343.
Pfam	PF02544. Steroid_dh. 1 hit. [Graphical view]
PIRSF	PIRSF015596. 5_alpha-SR2. 1 hit.
PROSITE	PS50244. S5A_REDUCTASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	604965.

Entry information

Entry name

S5A1_RAT

Accession

Primary (citable) accession number: P24008
Secondary accession number(s): A0JPJ6

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 1992
Last sequence update:	November 1, 1997
Last modified:	November 16, 2011
This is version 94 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program