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Protein

3-oxo-5-alpha-steroid 4-dehydrogenase 1

Gene

Srd5a1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Converts testosterone into 5-alpha-dihydrotestosterone and progesterone or corticosterone into their corresponding 5-alpha-3-oxosteroids. It plays a central role in sexual differentiation and androgen physiology.

Catalytic activityi

A 3-oxo-5-alpha-steroid + NADP+ = a 3-oxo-Delta(4)-steroid + NADPH.

pH dependencei

Optimally active at alkaline pHs.

GO - Molecular functioni

  1. 3-oxo-5-alpha-steroid 4-dehydrogenase activity Source: UniProtKB
  2. amide binding Source: RGD
  3. cholestenone 5-alpha-reductase activity Source: UniProtKB-EC
  4. NADPH binding Source: RGD

GO - Biological processi

  1. androgen biosynthetic process Source: RGD
  2. androgen metabolic process Source: RGD
  3. bone development Source: RGD
  4. cell differentiation Source: UniProtKB-KW
  5. cellular response to cAMP Source: RGD
  6. cellular response to dexamethasone stimulus Source: RGD
  7. cellular response to epinephrine stimulus Source: RGD
  8. cellular response to estradiol stimulus Source: RGD
  9. cellular response to growth factor stimulus Source: RGD
  10. cellular response to insulin stimulus Source: RGD
  11. cellular response to organic cyclic compound Source: RGD
  12. cellular response to starvation Source: RGD
  13. cellular response to testosterone stimulus Source: RGD
  14. cerebral cortex development Source: RGD
  15. circadian sleep/wake cycle, REM sleep Source: RGD
  16. diterpenoid metabolic process Source: RGD
  17. female genitalia development Source: RGD
  18. hippocampus development Source: RGD
  19. hypothalamus development Source: RGD
  20. liver development Source: RGD
  21. male genitalia development Source: RGD
  22. male gonad development Source: RGD
  23. male sex differentiation Source: RGD
  24. pituitary gland development Source: RGD
  25. progesterone metabolic process Source: RGD
  26. response to drug Source: RGD
  27. response to estradiol Source: RGD
  28. response to estrogen Source: RGD
  29. response to follicle-stimulating hormone Source: RGD
  30. response to fungicide Source: RGD
  31. response to growth hormone Source: RGD
  32. response to muscle activity Source: RGD
  33. response to organic cyclic compound Source: RGD
  34. response to testosterone Source: RGD
  35. serotonin metabolic process Source: RGD
  36. spinal cord development Source: RGD
  37. steroid biosynthetic process Source: RGD
  38. thalamus development Source: RGD
  39. urogenital system development Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Differentiation, Sexual differentiation

Keywords - Ligandi

NADP

Enzyme and pathway databases

BRENDAi1.3.1.22. 5301.

Names & Taxonomyi

Protein namesi
Recommended name:
3-oxo-5-alpha-steroid 4-dehydrogenase 1 (EC:1.3.1.22)
Alternative name(s):
SR type 1
Steroid 5-alpha-reductase 1
Short name:
S5AR 1
Gene namesi
Name:Srd5a1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Unplaced

Organism-specific databases

RGDi3757. Srd5a1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei10 – 3021HelicalSequence AnalysisAdd
BLAST
Transmembranei86 – 10621HelicalSequence AnalysisAdd
BLAST
Transmembranei111 – 13121HelicalSequence AnalysisAdd
BLAST
Transmembranei146 – 16621HelicalSequence AnalysisAdd
BLAST
Transmembranei206 – 22621HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. cell body fiber Source: RGD
  2. cytoplasm Source: RGD
  3. endoplasmic reticulum membrane Source: UniProtKB-SubCell
  4. integral component of membrane Source: UniProtKB-KW
  5. intracellular membrane-bounded organelle Source: RGD
  6. myelin sheath Source: RGD
  7. neuronal cell body Source: RGD
  8. perinuclear region of cytoplasm Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 2592593-oxo-5-alpha-steroid 4-dehydrogenase 1PRO_0000031565Add
BLAST

Proteomic databases

PaxDbiP24008.
PRIDEiP24008.

Expressioni

Tissue specificityi

Liver and prostate (at a low level).

Developmental stagei

After the establishment of chromosomal sex at fertilization.

Inductioni

Its expression is regulated by androgens such as testosterone.

Gene expression databases

GenevestigatoriP24008.

Interactioni

Protein-protein interaction databases

MINTiMINT-4570458.
STRINGi10116.ENSRNOP00000023659.

Family & Domainsi

Sequence similaritiesi

Belongs to the steroid 5-alpha reductase family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG82739.
HOGENOMiHOG000050133.
HOVERGENiHBG003402.
InParanoidiP24008.
KOiK12343.
PhylomeDBiP24008.

Family and domain databases

InterProiIPR016636. 3-oxo-5-alpha-steroid_4-DH.
IPR001104. 3-oxo-5_a-steroid_4-DH_C.
[Graphical view]
PfamiPF02544. Steroid_dh. 1 hit.
[Graphical view]
PIRSFiPIRSF015596. 5_alpha-SR2. 1 hit.
PROSITEiPS50244. S5A_REDUCTASE. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative initiation. AlignAdd to basket

Isoform Long (identifier: P24008-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVPLMELDEL CLLDMLVYLE GFMAFVSIVG LRSVGSPYGR YSPQWPGIRV
60 70 80 90 100
PARPAWFIQE LPSMAWPLYE YIRPAAARLG NLPNRVLLAM FLIHYVQRTL
110 120 130 140 150
VFPVLIRGGK PTLLVTFVLA FLFCTFNGYV QSRYLSQFAV YAEDWVTHPC
160 170 180 190 200
FLTGFALWLV GMVINIHSDH ILRNLRKPGE TGYKIPRGGL FEYVSAANYF
210 220 230 240 250
GELVEWCGFA LASWSLQGVV FALFTLSTLL TRAKQHHQWY HEKFEDYPKS

RKILIPFVL
Length:259
Mass (Da):29,780
Last modified:November 1, 1997 - v2
Checksum:iFE6705AC1D7CAE11
GO
Isoform Short (identifier: P24008-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-4: Missing.

Show »
Length:255
Mass (Da):29,339
Checksum:iA8510DF4235CB9CA
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 44Missing in isoform Short. 2 PublicationsVSP_018884

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J05035 mRNA. Translation: AAA42102.1.
S81448 mRNA. Translation: AAB36218.1.
BC127454 mRNA. Translation: AAI27455.1.
PIRiS65744.
RefSeqiNP_058766.2. NM_017070.3. [P24008-2]
UniGeneiRn.4620.

Genome annotation databases

GeneIDi24950.
KEGGirno:24950.
UCSCiRGD:3757. rat. [P24008-1]

Keywords - Coding sequence diversityi

Alternative initiation

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J05035 mRNA. Translation: AAA42102.1.
S81448 mRNA. Translation: AAB36218.1.
BC127454 mRNA. Translation: AAI27455.1.
PIRiS65744.
RefSeqiNP_058766.2. NM_017070.3. [P24008-2]
UniGeneiRn.4620.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-4570458.
STRINGi10116.ENSRNOP00000023659.

Chemistry

BindingDBiP24008.
ChEMBLiCHEMBL2096664.

Proteomic databases

PaxDbiP24008.
PRIDEiP24008.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi24950.
KEGGirno:24950.
UCSCiRGD:3757. rat. [P24008-1]

Organism-specific databases

CTDi6715.
RGDi3757. Srd5a1.

Phylogenomic databases

eggNOGiNOG82739.
HOGENOMiHOG000050133.
HOVERGENiHBG003402.
InParanoidiP24008.
KOiK12343.
PhylomeDBiP24008.

Enzyme and pathway databases

BRENDAi1.3.1.22. 5301.

Miscellaneous databases

NextBioi604965.

Gene expression databases

GenevestigatoriP24008.

Family and domain databases

InterProiIPR016636. 3-oxo-5-alpha-steroid_4-DH.
IPR001104. 3-oxo-5_a-steroid_4-DH_C.
[Graphical view]
PfamiPF02544. Steroid_dh. 1 hit.
[Graphical view]
PIRSFiPIRSF015596. 5_alpha-SR2. 1 hit.
PROSITEiPS50244. S5A_REDUCTASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Expression cloning and regulation of steroid 5 alpha-reductase, an enzyme essential for male sexual differentiation."
    Andersson S., Bishop R.W., Russell D.W.
    J. Biol. Chem. 264:16249-16255(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM SHORT).
  2. "Heterogeneity of rat type I 5 alpha-reductase cDNA: cloning, expression and regulation by pituitary implants and dihydrotestosterone."
    Lopez-Solache I., Luu-The V., Seralini G.E., Labrie F.
    Biochim. Biophys. Acta 1305:139-144(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM LONG).
    Tissue: Liver.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM SHORT).
    Tissue: Brain.

Entry informationi

Entry nameiS5A1_RAT
AccessioniPrimary (citable) accession number: P24008
Secondary accession number(s): A0JPJ6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: November 1, 1997
Last modified: April 1, 2015
This is version 119 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.