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P24008

- S5A1_RAT

UniProt

P24008 - S5A1_RAT

Protein

3-oxo-5-alpha-steroid 4-dehydrogenase 1

Gene

Srd5a1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 116 (01 Oct 2014)
      Sequence version 2 (01 Nov 1997)
      Previous versions | rss
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    Functioni

    Converts testosterone into 5-alpha-dihydrotestosterone and progesterone or corticosterone into their corresponding 5-alpha-3-oxosteroids. It plays a central role in sexual differentiation and androgen physiology.

    Catalytic activityi

    A 3-oxo-5-alpha-steroid + NADP+ = a 3-oxo-Delta(4)-steroid + NADPH.

    pH dependencei

    Optimally active at alkaline pHs.

    GO - Molecular functioni

    1. 3-oxo-5-alpha-steroid 4-dehydrogenase activity Source: UniProtKB
    2. amide binding Source: RGD
    3. cholestenone 5-alpha-reductase activity Source: UniProtKB-EC
    4. NADPH binding Source: RGD

    GO - Biological processi

    1. androgen biosynthetic process Source: RGD
    2. androgen metabolic process Source: RGD
    3. bone development Source: RGD
    4. cell differentiation Source: UniProtKB-KW
    5. cellular response to cAMP Source: RGD
    6. cellular response to dexamethasone stimulus Source: RGD
    7. cellular response to epinephrine stimulus Source: RGD
    8. cellular response to estradiol stimulus Source: RGD
    9. cellular response to growth factor stimulus Source: RGD
    10. cellular response to insulin stimulus Source: RGD
    11. cellular response to organic cyclic compound Source: RGD
    12. cellular response to starvation Source: RGD
    13. cellular response to testosterone stimulus Source: RGD
    14. cerebral cortex development Source: RGD
    15. circadian sleep/wake cycle, REM sleep Source: RGD
    16. diterpenoid metabolic process Source: RGD
    17. female genitalia development Source: RGD
    18. hippocampus development Source: RGD
    19. hypothalamus development Source: RGD
    20. liver development Source: RGD
    21. male genitalia development Source: RGD
    22. male gonad development Source: RGD
    23. male sex differentiation Source: RGD
    24. pituitary gland development Source: RGD
    25. progesterone metabolic process Source: RGD
    26. response to drug Source: RGD
    27. response to estradiol Source: RGD
    28. response to estrogen Source: RGD
    29. response to follicle-stimulating hormone Source: RGD
    30. response to fungicide Source: RGD
    31. response to growth hormone Source: RGD
    32. response to muscle activity Source: RGD
    33. response to organic cyclic compound Source: RGD
    34. response to testosterone Source: RGD
    35. serotonin metabolic process Source: RGD
    36. spinal cord development Source: RGD
    37. steroid biosynthetic process Source: RGD
    38. thalamus development Source: RGD
    39. urogenital system development Source: RGD

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Differentiation, Sexual differentiation

    Keywords - Ligandi

    NADP

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    3-oxo-5-alpha-steroid 4-dehydrogenase 1 (EC:1.3.1.22)
    Alternative name(s):
    SR type 1
    Steroid 5-alpha-reductase 1
    Short name:
    S5AR 1
    Gene namesi
    Name:Srd5a1
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi3757. Srd5a1.

    Subcellular locationi

    GO - Cellular componenti

    1. cell body fiber Source: RGD
    2. cytoplasm Source: RGD
    3. endoplasmic reticulum membrane Source: UniProtKB-SubCell
    4. integral component of membrane Source: UniProtKB-KW
    5. intracellular membrane-bounded organelle Source: RGD
    6. myelin sheath Source: RGD
    7. neuronal cell body Source: RGD
    8. perinuclear region of cytoplasm Source: RGD

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane, Microsome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 2592593-oxo-5-alpha-steroid 4-dehydrogenase 1PRO_0000031565Add
    BLAST

    Proteomic databases

    PaxDbiP24008.
    PRIDEiP24008.

    Expressioni

    Tissue specificityi

    Liver and prostate (at a low level).

    Developmental stagei

    After the establishment of chromosomal sex at fertilization.

    Inductioni

    Its expression is regulated by androgens such as testosterone.

    Gene expression databases

    GenevestigatoriP24008.

    Interactioni

    Protein-protein interaction databases

    MINTiMINT-4570458.
    STRINGi10116.ENSRNOP00000023659.

    Structurei

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei10 – 3021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei86 – 10621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei111 – 13121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei146 – 16621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei206 – 22621HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the steroid 5-alpha reductase family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG82739.
    HOGENOMiHOG000050133.
    HOVERGENiHBG003402.
    InParanoidiA0JPJ6.
    KOiK12343.
    PhylomeDBiP24008.

    Family and domain databases

    InterProiIPR016636. 3-oxo-5-alpha-steroid_4-DH.
    IPR001104. 3-oxo-5_a-steroid_4-DH_C.
    [Graphical view]
    PfamiPF02544. Steroid_dh. 1 hit.
    [Graphical view]
    PIRSFiPIRSF015596. 5_alpha-SR2. 1 hit.
    PROSITEiPS50244. S5A_REDUCTASE. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative initiation. Align

    Isoform Long (identifier: P24008-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MVPLMELDEL CLLDMLVYLE GFMAFVSIVG LRSVGSPYGR YSPQWPGIRV    50
    PARPAWFIQE LPSMAWPLYE YIRPAAARLG NLPNRVLLAM FLIHYVQRTL 100
    VFPVLIRGGK PTLLVTFVLA FLFCTFNGYV QSRYLSQFAV YAEDWVTHPC 150
    FLTGFALWLV GMVINIHSDH ILRNLRKPGE TGYKIPRGGL FEYVSAANYF 200
    GELVEWCGFA LASWSLQGVV FALFTLSTLL TRAKQHHQWY HEKFEDYPKS 250
    RKILIPFVL 259
    Length:259
    Mass (Da):29,780
    Last modified:November 1, 1997 - v2
    Checksum:iFE6705AC1D7CAE11
    GO
    Isoform Short (identifier: P24008-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-4: Missing.

    Show »
    Length:255
    Mass (Da):29,339
    Checksum:iA8510DF4235CB9CA
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 44Missing in isoform Short. 2 PublicationsVSP_018884

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J05035 mRNA. Translation: AAA42102.1.
    S81448 mRNA. Translation: AAB36218.1.
    BC127454 mRNA. Translation: AAI27455.1.
    PIRiS65744.
    RefSeqiNP_058766.2. NM_017070.3. [P24008-2]
    UniGeneiRn.4620.

    Genome annotation databases

    GeneIDi24950.
    KEGGirno:24950.
    UCSCiRGD:3757. rat. [P24008-1]

    Keywords - Coding sequence diversityi

    Alternative initiation

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J05035 mRNA. Translation: AAA42102.1 .
    S81448 mRNA. Translation: AAB36218.1 .
    BC127454 mRNA. Translation: AAI27455.1 .
    PIRi S65744.
    RefSeqi NP_058766.2. NM_017070.3. [P24008-2 ]
    UniGenei Rn.4620.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    MINTi MINT-4570458.
    STRINGi 10116.ENSRNOP00000023659.

    Chemistry

    BindingDBi P24008.
    ChEMBLi CHEMBL2096664.

    Proteomic databases

    PaxDbi P24008.
    PRIDEi P24008.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 24950.
    KEGGi rno:24950.
    UCSCi RGD:3757. rat. [P24008-1 ]

    Organism-specific databases

    CTDi 6715.
    RGDi 3757. Srd5a1.

    Phylogenomic databases

    eggNOGi NOG82739.
    HOGENOMi HOG000050133.
    HOVERGENi HBG003402.
    InParanoidi A0JPJ6.
    KOi K12343.
    PhylomeDBi P24008.

    Miscellaneous databases

    NextBioi 604965.

    Gene expression databases

    Genevestigatori P24008.

    Family and domain databases

    InterProi IPR016636. 3-oxo-5-alpha-steroid_4-DH.
    IPR001104. 3-oxo-5_a-steroid_4-DH_C.
    [Graphical view ]
    Pfami PF02544. Steroid_dh. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF015596. 5_alpha-SR2. 1 hit.
    PROSITEi PS50244. S5A_REDUCTASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Expression cloning and regulation of steroid 5 alpha-reductase, an enzyme essential for male sexual differentiation."
      Andersson S., Bishop R.W., Russell D.W.
      J. Biol. Chem. 264:16249-16255(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM SHORT).
    2. "Heterogeneity of rat type I 5 alpha-reductase cDNA: cloning, expression and regulation by pituitary implants and dihydrotestosterone."
      Lopez-Solache I., Luu-The V., Seralini G.E., Labrie F.
      Biochim. Biophys. Acta 1305:139-144(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM LONG).
      Tissue: Liver.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM SHORT).
      Tissue: Brain.

    Entry informationi

    Entry nameiS5A1_RAT
    AccessioniPrimary (citable) accession number: P24008
    Secondary accession number(s): A0JPJ6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 1, 1992
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 116 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3