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Protein

3-oxo-5-alpha-steroid 4-dehydrogenase 1

Gene

Srd5a1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Converts testosterone into 5-alpha-dihydrotestosterone and progesterone or corticosterone into their corresponding 5-alpha-3-oxosteroids. It plays a central role in sexual differentiation and androgen physiology.

Catalytic activityi

A 3-oxo-5-alpha-steroid + NADP+ = a 3-oxo-Delta(4)-steroid + NADPH.

pH dependencei

Optimally active at alkaline pHs.

GO - Molecular functioni

  • 3-oxo-5-alpha-steroid 4-dehydrogenase activity Source: UniProtKB
  • amide binding Source: RGD
  • cholestenone 5-alpha-reductase activity Source: UniProtKB-EC
  • NADPH binding Source: RGD

GO - Biological processi

  • androgen biosynthetic process Source: RGD
  • androgen metabolic process Source: RGD
  • bone development Source: RGD
  • cell differentiation Source: UniProtKB-KW
  • cellular response to cAMP Source: RGD
  • cellular response to dexamethasone stimulus Source: RGD
  • cellular response to epinephrine stimulus Source: RGD
  • cellular response to estradiol stimulus Source: RGD
  • cellular response to growth factor stimulus Source: RGD
  • cellular response to insulin stimulus Source: RGD
  • cellular response to organic cyclic compound Source: RGD
  • cellular response to starvation Source: RGD
  • cellular response to testosterone stimulus Source: RGD
  • cerebral cortex development Source: RGD
  • circadian sleep/wake cycle, REM sleep Source: RGD
  • diterpenoid metabolic process Source: RGD
  • female genitalia development Source: RGD
  • hippocampus development Source: RGD
  • hypothalamus development Source: RGD
  • liver development Source: RGD
  • male genitalia development Source: RGD
  • male gonad development Source: RGD
  • male sex differentiation Source: RGD
  • pituitary gland development Source: RGD
  • progesterone metabolic process Source: RGD
  • response to drug Source: RGD
  • response to estradiol Source: RGD
  • response to estrogen Source: RGD
  • response to follicle-stimulating hormone Source: RGD
  • response to fungicide Source: RGD
  • response to growth hormone Source: RGD
  • response to muscle activity Source: RGD
  • response to organic cyclic compound Source: RGD
  • response to testosterone Source: RGD
  • serotonin metabolic process Source: RGD
  • spinal cord development Source: RGD
  • steroid biosynthetic process Source: RGD
  • thalamus development Source: RGD
  • urogenital system development Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Differentiation, Sexual differentiation

Keywords - Ligandi

NADP

Enzyme and pathway databases

BRENDAi1.3.1.22. 5301.
ReactomeiR-RNO-193048. Androgen biosynthesis.

Names & Taxonomyi

Protein namesi
Recommended name:
3-oxo-5-alpha-steroid 4-dehydrogenase 1 (EC:1.3.1.22)
Alternative name(s):
SR type 1
Steroid 5-alpha-reductase 1
Short name:
S5AR 1
Gene namesi
Name:Srd5a1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 1

Organism-specific databases

RGDi3757. Srd5a1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei10 – 30HelicalSequence analysisAdd BLAST21
Transmembranei86 – 106HelicalSequence analysisAdd BLAST21
Transmembranei111 – 131HelicalSequence analysisAdd BLAST21
Transmembranei146 – 166HelicalSequence analysisAdd BLAST21
Transmembranei206 – 226HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

  • cell body fiber Source: RGD
  • cytoplasm Source: RGD
  • endoplasmic reticulum membrane Source: UniProtKB-SubCell
  • integral component of membrane Source: UniProtKB-KW
  • intracellular membrane-bounded organelle Source: RGD
  • myelin sheath Source: RGD
  • neuronal cell body Source: RGD
  • organelle membrane Source: UniProtKB-SubCell
  • perinuclear region of cytoplasm Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL3422.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000315651 – 2593-oxo-5-alpha-steroid 4-dehydrogenase 1Add BLAST259

Proteomic databases

PaxDbiP24008.
PRIDEiP24008.

Expressioni

Tissue specificityi

Liver and prostate (at a low level).

Developmental stagei

After the establishment of chromosomal sex at fertilization.

Inductioni

Its expression is regulated by androgens such as testosterone.

Gene expression databases

ExpressionAtlasiP24008. baseline and differential.

Interactioni

Protein-protein interaction databases

MINTiMINT-4570458.
STRINGi10116.ENSRNOP00000023659.

Chemistry databases

BindingDBiP24008.

Family & Domainsi

Sequence similaritiesi

Belongs to the steroid 5-alpha reductase family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1638. Eukaryota.
ENOG410XS6P. LUCA.
GeneTreeiENSGT00510000046634.
HOGENOMiHOG000050133.
HOVERGENiHBG003402.
InParanoidiP24008.
KOiK12343.
OMAiFEDYPKD.
PhylomeDBiP24008.

Family and domain databases

InterProiIPR016636. 3-oxo-5-alpha-steroid_4-DH.
IPR001104. 3-oxo-5_a-steroid_4-DH_C.
[Graphical view]
PfamiPF02544. Steroid_dh. 1 hit.
[Graphical view]
PIRSFiPIRSF015596. 5_alpha-SR2. 1 hit.
PROSITEiPS50244. S5A_REDUCTASE. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative initiation. AlignAdd to basket

Isoform Long (identifier: P24008-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVPLMELDEL CLLDMLVYLE GFMAFVSIVG LRSVGSPYGR YSPQWPGIRV
60 70 80 90 100
PARPAWFIQE LPSMAWPLYE YIRPAAARLG NLPNRVLLAM FLIHYVQRTL
110 120 130 140 150
VFPVLIRGGK PTLLVTFVLA FLFCTFNGYV QSRYLSQFAV YAEDWVTHPC
160 170 180 190 200
FLTGFALWLV GMVINIHSDH ILRNLRKPGE TGYKIPRGGL FEYVSAANYF
210 220 230 240 250
GELVEWCGFA LASWSLQGVV FALFTLSTLL TRAKQHHQWY HEKFEDYPKS

RKILIPFVL
Length:259
Mass (Da):29,780
Last modified:November 1, 1997 - v2
Checksum:iFE6705AC1D7CAE11
GO
Isoform Short (identifier: P24008-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-4: Missing.

Show »
Length:255
Mass (Da):29,339
Checksum:iA8510DF4235CB9CA
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0188841 – 4Missing in isoform Short. 2 Publications4

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J05035 mRNA. Translation: AAA42102.1.
S81448 mRNA. Translation: AAB36218.1.
BC127454 mRNA. Translation: AAI27455.1.
PIRiS65744.
RefSeqiNP_058766.2. NM_017070.3. [P24008-2]
UniGeneiRn.4620.

Genome annotation databases

EnsembliENSRNOT00000023659; ENSRNOP00000023659; ENSRNOG00000017601. [P24008-2]
GeneIDi24950.
KEGGirno:24950.
UCSCiRGD:3757. rat. [P24008-1]

Keywords - Coding sequence diversityi

Alternative initiation

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J05035 mRNA. Translation: AAA42102.1.
S81448 mRNA. Translation: AAB36218.1.
BC127454 mRNA. Translation: AAI27455.1.
PIRiS65744.
RefSeqiNP_058766.2. NM_017070.3. [P24008-2]
UniGeneiRn.4620.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-4570458.
STRINGi10116.ENSRNOP00000023659.

Chemistry databases

BindingDBiP24008.
ChEMBLiCHEMBL3422.

Proteomic databases

PaxDbiP24008.
PRIDEiP24008.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000023659; ENSRNOP00000023659; ENSRNOG00000017601. [P24008-2]
GeneIDi24950.
KEGGirno:24950.
UCSCiRGD:3757. rat. [P24008-1]

Organism-specific databases

CTDi6715.
RGDi3757. Srd5a1.

Phylogenomic databases

eggNOGiKOG1638. Eukaryota.
ENOG410XS6P. LUCA.
GeneTreeiENSGT00510000046634.
HOGENOMiHOG000050133.
HOVERGENiHBG003402.
InParanoidiP24008.
KOiK12343.
OMAiFEDYPKD.
PhylomeDBiP24008.

Enzyme and pathway databases

BRENDAi1.3.1.22. 5301.
ReactomeiR-RNO-193048. Androgen biosynthesis.

Miscellaneous databases

PROiP24008.

Gene expression databases

ExpressionAtlasiP24008. baseline and differential.

Family and domain databases

InterProiIPR016636. 3-oxo-5-alpha-steroid_4-DH.
IPR001104. 3-oxo-5_a-steroid_4-DH_C.
[Graphical view]
PfamiPF02544. Steroid_dh. 1 hit.
[Graphical view]
PIRSFiPIRSF015596. 5_alpha-SR2. 1 hit.
PROSITEiPS50244. S5A_REDUCTASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiS5A1_RAT
AccessioniPrimary (citable) accession number: P24008
Secondary accession number(s): A0JPJ6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: November 1, 1997
Last modified: October 5, 2016
This is version 128 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.