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P24008 (S5A1_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 114. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
3-oxo-5-alpha-steroid 4-dehydrogenase 1

EC=1.3.1.22
Alternative name(s):
SR type 1
Steroid 5-alpha-reductase 1
Short name=S5AR 1
Gene names
Name:Srd5a1
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length259 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Converts testosterone into 5-alpha-dihydrotestosterone and progesterone or corticosterone into their corresponding 5-alpha-3-oxosteroids. It plays a central role in sexual differentiation and androgen physiology.

Catalytic activity

A 3-oxo-5-alpha-steroid + NADP+ = a 3-oxo-Delta(4)-steroid + NADPH.

Subcellular location

Microsome membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein Potential.

Tissue specificity

Liver and prostate (at a low level).

Developmental stage

After the establishment of chromosomal sex at fertilization.

Induction

Its expression is regulated by androgens such as testosterone.

Sequence similarities

Belongs to the steroid 5-alpha reductase family.

Biophysicochemical properties

pH dependence:

Optimally active at alkaline pHs.

Ontologies

Keywords
   Biological processDifferentiation
Sexual differentiation
   Cellular componentEndoplasmic reticulum
Membrane
Microsome
   Coding sequence diversityAlternative initiation
   DomainTransmembrane
Transmembrane helix
   LigandNADP
   Molecular functionOxidoreductase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processandrogen biosynthetic process

Inferred from direct assay PubMed 12606426. Source: RGD

androgen metabolic process

Inferred from direct assay PubMed 20954080. Source: RGD

bone development

Inferred from expression pattern PubMed 15012600. Source: RGD

cell differentiation

Inferred from electronic annotation. Source: UniProtKB-KW

cellular response to cAMP

Inferred from expression pattern PubMed 14997014. Source: RGD

cellular response to dexamethasone stimulus

Inferred from expression pattern PubMed 15004407. Source: RGD

cellular response to epinephrine stimulus

Inferred from expression pattern PubMed 14997014. Source: RGD

cellular response to estradiol stimulus

Inferred from expression pattern PubMed 15312799. Source: RGD

cellular response to growth factor stimulus

Inferred from expression pattern PubMed 15249139. Source: RGD

cellular response to insulin stimulus

Inferred from expression pattern PubMed 20810561. Source: RGD

cellular response to organic cyclic compound

Inferred from expression pattern PubMed 15936112. Source: RGD

cellular response to starvation

Inferred from expression pattern PubMed 9729333. Source: RGD

cellular response to testosterone stimulus

Inferred from expression pattern PubMed 15004407. Source: RGD

cerebral cortex development

Inferred from expression pattern PubMed 8770891. Source: RGD

circadian sleep/wake cycle, REM sleep

Inferred from expression pattern PubMed 12597996. Source: RGD

diterpenoid metabolic process

Inferred from expression pattern PubMed 14997014. Source: RGD

female genitalia development

Inferred from expression pattern PubMed 9142501. Source: RGD

hippocampus development

Inferred from expression pattern PubMed 8770891. Source: RGD

hypothalamus development

Inferred from expression pattern PubMed 15804399PubMed 8770891. Source: RGD

liver development

Inferred from expression pattern PubMed 8770891. Source: RGD

male genitalia development

Inferred from expression pattern PubMed 9142501. Source: RGD

male gonad development

Inferred from expression pattern PubMed 12606426. Source: RGD

male sex differentiation

Traceable author statement Ref.1. Source: RGD

pituitary gland development

Inferred from expression pattern PubMed 8770891. Source: RGD

progesterone metabolic process

Inferred from direct assay PubMed 9202401. Source: RGD

response to drug

Inferred from mutant phenotype PubMed 1314830. Source: RGD

response to estradiol

Inferred from expression pattern PubMed 9504773. Source: RGD

response to estrogen

Inferred from expression pattern PubMed 19793540. Source: RGD

response to follicle-stimulating hormone

Inferred from expression pattern PubMed 12630924. Source: RGD

response to fungicide

Inferred from expression pattern PubMed 19728174. Source: RGD

response to growth hormone

Inferred from expression pattern PubMed 10067850. Source: RGD

response to muscle activity

Inferred from expression pattern PubMed 20045012. Source: RGD

response to organic cyclic compound

Inferred from expression pattern PubMed 17940878. Source: RGD

response to testosterone

Inferred from expression pattern PubMed 16595696. Source: RGD

serotonin metabolic process

Inferred from expression pattern PubMed 15936112. Source: RGD

spinal cord development

Inferred from expression pattern PubMed 8770891. Source: RGD

steroid biosynthetic process

Inferred from direct assay PubMed 7482629. Source: RGD

thalamus development

Inferred from expression pattern PubMed 8770891. Source: RGD

urogenital system development

Inferred from expression pattern PubMed 8584033. Source: RGD

   Cellular_componentcell body fiber

Inferred from direct assay PubMed 15305365. Source: RGD

cytoplasm

Inferred from direct assay PubMed 15305365. Source: RGD

endoplasmic reticulum membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

integral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

intracellular membrane-bounded organelle

Inferred from direct assay PubMed 20724274. Source: RGD

myelin sheath

Inferred from direct assay PubMed 9202401. Source: RGD

neuronal cell body

Inferred from direct assay PubMed 15305365. Source: RGD

perinuclear region of cytoplasm

Inferred from direct assay PubMed 7553073. Source: RGD

   Molecular_function3-oxo-5-alpha-steroid 4-dehydrogenase activity

Inferred from sequence or structural similarity. Source: UniProtKB

NADPH binding

Inferred from direct assay PubMed 11377984. Source: RGD

amide binding

Inferred from direct assay PubMed 9090798. Source: RGD

cholestenone 5-alpha-reductase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative initiation. [Align] [Select]
Isoform Long (identifier: P24008-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform Short (identifier: P24008-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-4: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 2592593-oxo-5-alpha-steroid 4-dehydrogenase 1
PRO_0000031565

Regions

Transmembrane10 – 3021Helical; Potential
Transmembrane86 – 10621Helical; Potential
Transmembrane111 – 13121Helical; Potential
Transmembrane146 – 16621Helical; Potential
Transmembrane206 – 22621Helical; Potential

Natural variations

Alternative sequence1 – 44Missing in isoform Short.
VSP_018884

Sequences

Sequence LengthMass (Da)Tools
Isoform Long [UniParc].

Last modified November 1, 1997. Version 2.
Checksum: FE6705AC1D7CAE11

FASTA25929,780
        10         20         30         40         50         60 
MVPLMELDEL CLLDMLVYLE GFMAFVSIVG LRSVGSPYGR YSPQWPGIRV PARPAWFIQE 

        70         80         90        100        110        120 
LPSMAWPLYE YIRPAAARLG NLPNRVLLAM FLIHYVQRTL VFPVLIRGGK PTLLVTFVLA 

       130        140        150        160        170        180 
FLFCTFNGYV QSRYLSQFAV YAEDWVTHPC FLTGFALWLV GMVINIHSDH ILRNLRKPGE 

       190        200        210        220        230        240 
TGYKIPRGGL FEYVSAANYF GELVEWCGFA LASWSLQGVV FALFTLSTLL TRAKQHHQWY 

       250 
HEKFEDYPKS RKILIPFVL 

« Hide

Isoform Short [UniParc].

Checksum: A8510DF4235CB9CA
Show »

FASTA25529,339

References

« Hide 'large scale' references
[1]"Expression cloning and regulation of steroid 5 alpha-reductase, an enzyme essential for male sexual differentiation."
Andersson S., Bishop R.W., Russell D.W.
J. Biol. Chem. 264:16249-16255(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM SHORT).
[2]"Heterogeneity of rat type I 5 alpha-reductase cDNA: cloning, expression and regulation by pituitary implants and dihydrotestosterone."
Lopez-Solache I., Luu-The V., Seralini G.E., Labrie F.
Biochim. Biophys. Acta 1305:139-144(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM LONG).
Tissue: Liver.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM SHORT).
Tissue: Brain.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
J05035 mRNA. Translation: AAA42102.1.
S81448 mRNA. Translation: AAB36218.1.
BC127454 mRNA. Translation: AAI27455.1.
PIRS65744.
RefSeqNP_058766.2. NM_017070.3.
UniGeneRn.4620.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

MINTMINT-4570458.
STRING10116.ENSRNOP00000023659.

Chemistry

BindingDBP24008.
ChEMBLCHEMBL2096664.

Proteomic databases

PaxDbP24008.
PRIDEP24008.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID24950.
KEGGrno:24950.
UCSCRGD:3757. rat. [P24008-1]

Organism-specific databases

CTD6715.
RGD3757. Srd5a1.

Phylogenomic databases

eggNOGNOG82739.
HOGENOMHOG000050133.
HOVERGENHBG003402.
InParanoidA0JPJ6.
KOK12343.
PhylomeDBP24008.

Gene expression databases

GenevestigatorP24008.

Family and domain databases

InterProIPR016636. 3-oxo-5-alpha-steroid_4-DH.
IPR001104. 3-oxo-5_a-steroid_4-DH_C.
[Graphical view]
PfamPF02544. Steroid_dh. 1 hit.
[Graphical view]
PIRSFPIRSF015596. 5_alpha-SR2. 1 hit.
PROSITEPS50244. S5A_REDUCTASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio604965.

Entry information

Entry nameS5A1_RAT
AccessionPrimary (citable) accession number: P24008
Secondary accession number(s): A0JPJ6
Entry history
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: November 1, 1997
Last modified: April 16, 2014
This is version 114 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families