P24005 (ACTB_DICDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Calcium-regulated actin-bundling protein

Alternative name(s):
34 kDa actin-binding protein

Gene names

Name:	abpB
ORF Names:	DDB_G0279081

Organism

Dictyostelium discoideum (Slime mold)

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	295 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May contribute to the structure and reorganization of filopodia and pseudopodia accompanying cell movements.
Subunit structure	Monomer.

Ontologies

Keywords
Ligand	Actin-binding Calcium
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Cellular component	cortical cytoskeleton Inferred from direct assay. Source: dictyBase phagocytic vesicle Inferred from direct assay. Source: dictyBase
Molecular function	actin binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 295	295	Calcium-regulated actin-bundling protein		PRO_0000073833
Regions
	Compositional bias	256 – 275	20	Ala/Gly/Ser-rich
	Compositional bias	286 – 295	10	Arg/Lys-rich (basic)

Sequences

	Sequence		Length	Mass (Da)
	P24005 [UniParc]. Last modified March 1, 1992. Version 1. Checksum: 1BBF85DEA657097D	FASTA	295	33,353
	10 20 30 40 50 60 MAETKVAPNL TGIEQTKAGQ SFTEKLSAEA MEFFCNVAKL PFSQQAVHFL NAYWAEVSKE 70 80 90 100 110 120 AEFIYSVGWE TIKYADMHCK GIQLVFKYDE GNDLDFDIAL YFYEQLCKFC EDPKNKNYAT 130 140 150 160 170 180 TYPISQPQML TALKRKQELR EKVDVNFDGR VSFLEYLLYQ YKDFANPADF CTRSMNHDEH 190 200 210 220 230 240 PEIKKARLAL EEVNKRIRAY EEEKARLTEE SKIPGVKGLG ATNMLAQIDS GPLKEQLNFA 250 260 270 280 290 LISAEAAVRT ASKKYGGAAY SGGAGDAGAG SSAGAIWWMN RDLEEKKKRY GPQKK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation and sequencing of cDNA clones encoding the Dictyostelium discoideum 30,000-dalton actin-bundling protein." Fechheimer M., Murdock D., Carney M., Glover C.V.C. J. Biol. Chem. 266:2883-2889(1991) [PubMed: 1993662] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 7-22 AND 225-237.
[2]	"Dictyostelium discoideum cells lacking the 34,000-dalton actin-binding protein can grow, locomote, and develop, but exhibit defects in regulation of cell structure and movement: a case of partial redundancy." Rivero F., Furukawa R., Noegel A.A., Fechheimer M. J. Cell Biol. 135:965-980(1996) [PubMed: 8922380] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: AX3.
[3]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[4]	"Identification and isolation of Dictyostelium microtubule-associated protein interactors by tandem affinity purification." Koch K.V., Reinders Y., Ho T.-H., Sickmann A., Graef R. Eur. J. Cell Biol. 85:1079-1090(2006) [PubMed: 16782229] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Strain: AX2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M58022 mRNA. Translation: AAA33144.1. Z50156 Genomic DNA. Translation: CAA90518.1. U32112 Genomic DNA. Translation: AAB48216.1. AAFI02000027 Genomic DNA. Translation: EAL67879.1.
PIR	A23750.
RefSeq	XP_641862.1. XM_636770.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblProtists	DDB0214810; DDB0214810; DDB_G0279081.
GeneID	8621868.
GenomeReviews	Gene locus abpB in contig CM000152_GR.
KEGG	ddi:DDB_G0279081.
Organism-specific databases
dictyBase	DDB_G0279081. abpB.
Family and domain databases
ProtoNet	Search...

Entry information

Entry name

ACTB_DICDI

Accession

Primary (citable) accession number: P24005
Secondary accession number(s): Q54XA5

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 1992
Last sequence update:	March 1, 1992
Last modified:	September 21, 2011
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)