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Protein

Peroxisomal ATPase PEX1

Gene

PEX1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the peroxisomal protein import machinery. Together with PEX6, mediates the ATP-dependent relocation and recycling of the peroxisomal targeting signal-1 (PTS1) import receptor PEX5 from the peroxisomal membrane to the cytosol, where it is then available for another round of protein import into the organelle.3 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi461 – 468ATPSequence analysis8
Nucleotide bindingi738 – 745ATPSequence analysis8

GO - Molecular functioni

  • ATPase activity Source: SGD
  • ATPase activity, coupled Source: InterPro
  • ATP binding Source: UniProtKB-KW
  • protein heterodimerization activity Source: SGD

GO - Biological processi

  • protein import into peroxisome matrix, receptor recycling Source: SGD
Complete GO annotation...

Keywords - Biological processi

Peroxisome biogenesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-31959-MONOMER.

Protein family/group databases

TCDBi3.A.20.1.5. the peroxisomal protein importer (ppi) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Peroxisomal ATPase PEX1
Alternative name(s):
Peroxin-1
Peroxisomal assembly protein 1
Peroxisome biogenesis protein PAS1
Gene namesi
Name:PEX1
Synonyms:PAS1
Ordered Locus Names:YKL197C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XI

Organism-specific databases

EuPathDBiFungiDB:YKL197C.
SGDiS000001680. PEX1.

Subcellular locationi

GO - Cellular componenti

  • peroxisomal membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane, Peroxisome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi467K → E in PEX1pA1; no effect. 3 Publications1
Mutagenesisi525D → Q in PEX1pB1; no effect. 2 Publications1
Mutagenesisi744K → E in PEX1pA2; decreased binding to PEX6. Results in accumulation of PEX5 on peroxisomal membranes. 3 Publications1
Mutagenesisi797D → Q in PEX1pB2; results in accumulation of PEX5 on peroxisomal membranes. 2 Publications1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000846061 – 1043Peroxisomal ATPase PEX1Add BLAST1043

Proteomic databases

MaxQBiP24004.
PRIDEiP24004.

Expressioni

Inductioni

By oleate.

Interactioni

Subunit structurei

Interacts with PEX6; forms a high-molecular-mass heterooligomer in the cytosol. The PEX1-PEX6 heterooligomers associate with the peroxisomal importomer via interaction of PEX6 with the peroxisomal membrane anchor PEX15.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
PEX6P3376016EBI-13155,EBI-13178

GO - Molecular functioni

  • protein heterodimerization activity Source: SGD

Protein-protein interaction databases

BioGridi33923. 55 interactors.
DIPiDIP-4266N.
IntActiP24004. 8 interactors.
MINTiMINT-483826.

Structurei

3D structure databases

ProteinModelPortaliP24004.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni453 – 626AAA-cassette D1Add BLAST174
Regioni733 – 926AAA-cassette D2Add BLAST194

Domaini

AAA-cassette D1 is required for interaction with PEX6. ATP-binding in AAA-cassette D2 is required for interaction with PEX6. ATP-binding and hydrolysis in AAA-cassette D2 is required for proper function in PEX5 dislocation.

Sequence similaritiesi

Belongs to the AAA ATPase family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

GeneTreeiENSGT00550000075032.
HOGENOMiHOG000163138.
InParanoidiP24004.
KOiK13338.
OMAiSAVAQQC.
OrthoDBiEOG092C0WJM.

Family and domain databases

Gene3Di3.10.330.10. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR003960. ATPase_AAA_CS.
IPR029067. CDC48_domain_2-like.
IPR027417. P-loop_NTPase.
IPR015342. PEX-N_psi_beta-barrel.
IPR025653. Pex1.
[Graphical view]
PANTHERiPTHR23077:SF12. PTHR23077:SF12. 3 hits.
PfamiPF00004. AAA. 2 hits.
PF09262. PEX-1N. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 2 hits.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
SSF54585. SSF54585. 1 hit.
PROSITEiPS00674. AAA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P24004-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTTKRLKFE NLRIQFSNAI VGNFLRLPHS IINVLESTNY AIQEFGIAVH
60 70 80 90 100
SHNSDIPIVH LGWDGHDSGS SENVVLINPV LATVYDLNQK SPLVDLYIQR
110 120 130 140 150
YDHTHLATEV YVTPETSDDW EIIDANAMRF QNGEILHQTR IVTPGETLIC
160 170 180 190 200
YLEGIVTKFK IDRVEPSMKS ARITDGSLVV VAPKVNKTRL VKAEYGHSNK
210 220 230 240 250
TILKNGAIQL LKKVILRSTV CKMDFPKDNL FVVYISDGAQ LPSQKGYASI
260 270 280 290 300
VKCSLRQSKK SDSDNKSVGI PSKKIGVFIK CDSQIPENHI ALSSHLWDAF
310 320 330 340 350
FTHPMNGAKI KLEFLQMNQA NIISGRNATV NIKYFGKDVP TKSGDQYSKL
360 370 380 390 400
LGGSLLTNNL ILPTEQIIIE IKKGESEQQL CNLNEISNES VQWKVTQMGK
410 420 430 440 450
EEVKDIIERH LPKHYHVKET GEVSRTSKDE DDFITVNSIK KEMVNYLTSP
460 470 480 490 500
IIATPAIILD GKQGIGKTRL LKELINEVEK DHHIFVKYAD CETLHETSNL
510 520 530 540 550
DKTQKLIMEW CSFCYWYGPS LIVLDNVEAL FGKPQANDGD PSNNGQWDNA
560 570 580 590 600
SKLLNFFINQ VTKIFNKDNK RIRVLFSGKQ KTQINPLLFD KHFVSETWSL
610 620 630 640 650
RAPDKHARAK LLEYFFSKNQ IMKLNRDLQF SDLSLETEGF SPLDLEIFTE
660 670 680 690 700
KIFYDLQLER DCDNVVTREL FSKSLSAFTP SALRGVKLTK ETNIKWGDIG
710 720 730 740 750
ALANAKDVLL ETLEWPTKYE PIFVNCPLRL RSGILLYGYP GCGKTLLASA
760 770 780 790 800
VAQQCGLNFI SVKGPEILNK FIGASEQNIR ELFERAQSVK PCILFFDEFD
810 820 830 840 850
SIAPKRGHDS TGVTDRVVNQ LLTQMDGAEG LDGVYILAAT SRPDLIDSAL
860 870 880 890 900
LRPGRLDKSV ICNIPTESER LDILQAIVNS KDKDTGQKKF ALEKNADLKL
910 920 930 940 950
IAEKTAGFSG ADLQGLCYNA YLKSVHRWLS AADQSEVVPG NDNIEYFSIN
960 970 980 990 1000
EHGRREENRL RLKTLLQQDV VHETKTSTSA ASELTAVVTI NDLLEACQET
1010 1020 1030 1040
KPSISTSELV KLRGIYDRFQ KDRNGEMPNG ENSIDIGSRL SLM
Length:1,043
Mass (Da):117,276
Last modified:June 1, 1994 - v2
Checksum:iEB13C080FB8189DB
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti354S → T in AAA34842 (PubMed:1825027).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M58676 Genomic DNA. Translation: AAA34842.1.
Z28197 Genomic DNA. Translation: CAA82041.1.
BK006944 Genomic DNA. Translation: DAA08969.1.
PIRiS38034.
RefSeqiNP_012724.1. NM_001179763.1.

Genome annotation databases

EnsemblFungiiYKL197C; YKL197C; YKL197C.
GeneIDi853636.
KEGGisce:YKL197C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M58676 Genomic DNA. Translation: AAA34842.1.
Z28197 Genomic DNA. Translation: CAA82041.1.
BK006944 Genomic DNA. Translation: DAA08969.1.
PIRiS38034.
RefSeqiNP_012724.1. NM_001179763.1.

3D structure databases

ProteinModelPortaliP24004.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33923. 55 interactors.
DIPiDIP-4266N.
IntActiP24004. 8 interactors.
MINTiMINT-483826.

Protein family/group databases

TCDBi3.A.20.1.5. the peroxisomal protein importer (ppi) family.

Proteomic databases

MaxQBiP24004.
PRIDEiP24004.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYKL197C; YKL197C; YKL197C.
GeneIDi853636.
KEGGisce:YKL197C.

Organism-specific databases

EuPathDBiFungiDB:YKL197C.
SGDiS000001680. PEX1.

Phylogenomic databases

GeneTreeiENSGT00550000075032.
HOGENOMiHOG000163138.
InParanoidiP24004.
KOiK13338.
OMAiSAVAQQC.
OrthoDBiEOG092C0WJM.

Enzyme and pathway databases

BioCyciYEAST:G3O-31959-MONOMER.

Miscellaneous databases

PROiP24004.

Family and domain databases

Gene3Di3.10.330.10. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR003960. ATPase_AAA_CS.
IPR029067. CDC48_domain_2-like.
IPR027417. P-loop_NTPase.
IPR015342. PEX-N_psi_beta-barrel.
IPR025653. Pex1.
[Graphical view]
PANTHERiPTHR23077:SF12. PTHR23077:SF12. 3 hits.
PfamiPF00004. AAA. 2 hits.
PF09262. PEX-1N. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 2 hits.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
SSF54585. SSF54585. 1 hit.
PROSITEiPS00674. AAA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPEX1_YEAST
AccessioniPrimary (citable) accession number: P24004
Secondary accession number(s): D6VX03
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: June 1, 1994
Last modified: November 30, 2016
This is version 151 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2100 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.