P24004 (PEX1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Peroxisomal ATPase PEX1

Alternative name(s):
Peroxin-1
Peroxisomal assembly protein 1
Peroxisome biogenesis protein PAS1

Gene names

Name:	PEX1
Synonyms:	PAS1
Ordered Locus Names:	YKL197C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	1043 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the peroxisomal protein import machinery. Together with PEX6, mediates the ATP-dependent relocation and recycling of the peroxisomal targeting signal-1 (PTS1) import receptor PEX5 from the peroxisomal membrane to the cytosol, where it is then available for another round of protein import into the organelle. Ref.7 Ref.8 Ref.9
Subunit structure	Interacts with PEX6; forms a high-molcular-mass heterooligomer in the cytosol. The PEX1-PEX6 heterooligomers associate with the peroxisomal importomer via interaction of PEX6 with the peroxisomal membrane anchor PEX15. Ref.7 Ref.8 Ref.9
Subcellular location	Cytoplasm. Peroxisome membrane; Peripheral membrane protein; Cytoplasmic side. Note: Shuttles between the cytoplasm and the peroxisomal membrane. Ref.5
Induction	By oleate.
Domain	AAA-cassette D1 is required for interaction with PEX6. ATP-binding in AAA-cassette D2 is required for interaction with PEX6. ATP-binding and hydrolysis in AAA-cassette D2 is required for proper function in PEX5 dislocation.
Miscellaneous	Present with 2100 molecules/cell in log phase SD medium. Ref.6
Sequence similarities	Belongs to the AAA ATPase family.

Ontologies

Keywords
Biological process	Peroxisome biogenesis
Cellular component	Cytoplasm Membrane Peroxisome
Domain	Repeat
Ligand	ATP-binding Nucleotide-binding
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	protein import into peroxisome matrix, receptor recycling Inferred from mutant phenotype Ref.8. Source: SGD
Cellular component	membrane fraction Inferred from direct assay Ref.9. Source: SGD peroxisomal membrane Inferred from electronic annotation. Source: UniProtKB-SubCell soluble fraction Inferred from direct assay Ref.9. Source: SGD
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATPase activity Inferred from mutant phenotype Ref.7. Source: SGD protein heterodimerization activity Inferred from mutant phenotype Ref.7. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
PEX6	P33760	3	EBI-13155,EBI-13178

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1043	1043	Peroxisomal ATPase PEX1		PRO_0000084606
Regions
	Nucleotide binding	461 – 468	8	ATP Potential
	Nucleotide binding	738 – 745	8	ATP Potential
	Region	453 – 626	174	AAA-cassette D1
	Region	733 – 926	194	AAA-cassette D2
Experimental info
	Mutagenesis	467	1	K → E in PEX1pA1; no effect. Ref.4 Ref.7 Ref.8
	Mutagenesis	525	1	D → Q in PEX1pB1; no effect. Ref.7 Ref.8
	Mutagenesis	744	1	K → E in PEX1pA2; decreased binding to PEX6. Results in accumulation of PEX5 on peroxisomal membranes. Ref.4 Ref.7 Ref.8
	Mutagenesis	797	1	D → Q in PEX1pB2; results in accumulation of PEX5 on peroxisomal membranes. Ref.7 Ref.8
	Sequence conflict	354	1	S → T in AAA34842. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P24004 [UniParc]. Last modified June 1, 1994. Version 2. Checksum: EB13C080FB8189DB	FASTA	1,043	117,276
	10 20 30 40 50 60 MTTTKRLKFE NLRIQFSNAI VGNFLRLPHS IINVLESTNY AIQEFGIAVH SHNSDIPIVH 70 80 90 100 110 120 LGWDGHDSGS SENVVLINPV LATVYDLNQK SPLVDLYIQR YDHTHLATEV YVTPETSDDW 130 140 150 160 170 180 EIIDANAMRF QNGEILHQTR IVTPGETLIC YLEGIVTKFK IDRVEPSMKS ARITDGSLVV 190 200 210 220 230 240 VAPKVNKTRL VKAEYGHSNK TILKNGAIQL LKKVILRSTV CKMDFPKDNL FVVYISDGAQ 250 260 270 280 290 300 LPSQKGYASI VKCSLRQSKK SDSDNKSVGI PSKKIGVFIK CDSQIPENHI ALSSHLWDAF 310 320 330 340 350 360 FTHPMNGAKI KLEFLQMNQA NIISGRNATV NIKYFGKDVP TKSGDQYSKL LGGSLLTNNL 370 380 390 400 410 420 ILPTEQIIIE IKKGESEQQL CNLNEISNES VQWKVTQMGK EEVKDIIERH LPKHYHVKET 430 440 450 460 470 480 GEVSRTSKDE DDFITVNSIK KEMVNYLTSP IIATPAIILD GKQGIGKTRL LKELINEVEK 490 500 510 520 530 540 DHHIFVKYAD CETLHETSNL DKTQKLIMEW CSFCYWYGPS LIVLDNVEAL FGKPQANDGD 550 560 570 580 590 600 PSNNGQWDNA SKLLNFFINQ VTKIFNKDNK RIRVLFSGKQ KTQINPLLFD KHFVSETWSL 610 620 630 640 650 660 RAPDKHARAK LLEYFFSKNQ IMKLNRDLQF SDLSLETEGF SPLDLEIFTE KIFYDLQLER 670 680 690 700 710 720 DCDNVVTREL FSKSLSAFTP SALRGVKLTK ETNIKWGDIG ALANAKDVLL ETLEWPTKYE 730 740 750 760 770 780 PIFVNCPLRL RSGILLYGYP GCGKTLLASA VAQQCGLNFI SVKGPEILNK FIGASEQNIR 790 800 810 820 830 840 ELFERAQSVK PCILFFDEFD SIAPKRGHDS TGVTDRVVNQ LLTQMDGAEG LDGVYILAAT 850 860 870 880 890 900 SRPDLIDSAL LRPGRLDKSV ICNIPTESER LDILQAIVNS KDKDTGQKKF ALEKNADLKL 910 920 930 940 950 960 IAEKTAGFSG ADLQGLCYNA YLKSVHRWLS AADQSEVVPG NDNIEYFSIN EHGRREENRL 970 980 990 1000 1010 1020 RLKTLLQQDV VHETKTSTSA ASELTAVVTI NDLLEACQET KPSISTSELV KLRGIYDRFQ 1030 1040 KDRNGEMPNG ENSIDIGSRL SLM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"PAS1, a yeast gene required for peroxisome biogenesis, encodes a member of a novel family of putative ATPases." Erdmann R., Wiebel F.F., Flessau A., Rytka J., Beyer A., Froehlich K.-U., Kunau W.-H. Cell 64:499-510(1991) [PubMed: 1825027] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete DNA sequence of yeast chromosome XI." Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W. Nature 369:371-378(1994) [PubMed: 8196765] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Effect of site-directed mutagenesis of conserved lysine residues upon Pas1 protein function in peroxisome biogenesis." Krause T., Kunau W.-H., Erdmann R. Yeast 10:1613-1620(1994) [PubMed: 7725796] [Abstract] Cited for: MUTAGENESIS OF LYS-467 AND LYS-744.
[5]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"Structural and functional analysis of the interaction of the AAA-peroxins Pex1p and Pex6p." Birschmann I., Rosenkranz K., Erdmann R., Kunau W.-H. FEBS J. 272:47-58(2005) [PubMed: 15634331] [Abstract] Cited for: FUNCTION, INTERACTION WITH PEX6, MUTAGENESIS OF LYS-467; ASP-525; LYS-744 AND ASP-797.
[8]	"Functional role of the AAA peroxins in dislocation of the cycling PTS1 receptor back to the cytosol." Platta H.W., Grunau S., Rosenkranz K., Girzalsky W., Erdmann R. Nat. Cell Biol. 7:817-822(2005) [PubMed: 16007078] [Abstract] Cited for: FUNCTION, INTERACTION WITH PEX6, MUTAGENESIS OF LYS-467; ASP-525; LYS-744 AND ASP-797.
[9]	"Functional association of the AAA complex and the peroxisomal importomer." Rosenkranz K., Birschmann I., Grunau S., Girzalsky W., Kunau W.-H., Erdmann R. FEBS J. 273:3804-3815(2006) [PubMed: 16911527] [Abstract] Cited for: FUNCTION, INTERACTION WITH PEX6.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M58676 Genomic DNA. Translation: AAA34842.1. Z28197 Genomic DNA. Translation: CAA82041.1. BK006944 Genomic DNA. Translation: DAA08969.1.
PIR	S38034.
RefSeq	NP_012724.1. NM_001179763.1.
3D structure databases
ProteinModelPortal	P24004.
SMR	P24004. Positions 12-183, 454-495, 691-1004.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-4266N.
IntAct	P24004. 1 interaction.
MINT	MINT-483826.
STRING	P24004.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YKL197C; YKL197C; YKL197C.
GeneID	853636.
KEGG	sce:YKL197C.
NMPDR	fig|4932.3.peg.3701.
Organism-specific databases
CYGD	YKL197c.
SGD	S000001680. PEX1.
Phylogenomic databases
eggNOG	fuNOG04405.
GeneTree	EFGT00050000000010.
HOGENOM	HBG202371.
OMA	SAVAQQC.
OrthoDB	EOG4TQQJ3.
Gene expression databases
ArrayExpress	P24004.
Genevestigator	P24004.
GermOnline	YKL197C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR003593. ATPase_AAA+_core. IPR003959. ATPase_AAA_core. IPR003960. ATPase_AAA_CS. IPR015342. PEX-1N. [Graphical view]
KO	K13338.
Pfam	PF00004. AAA. 2 hits. PF09262. PEX-1N. 1 hit. [Graphical view]
SMART	SM00382. AAA. 2 hits. [Graphical view]
PROSITE	PS00674. AAA. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	974520.

Entry information

Entry name

PEX1_YEAST

Accession

Primary (citable) accession number: P24004
Secondary accession number(s): D6VX03

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 1992
Last sequence update:	June 1, 1994
Last modified:	December 14, 2011
This is version 103 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program