Reviewed, UniProtKB/Swiss-Prot P24001 (IL32_HUMAN)

Names and origin

Protein names

Recommended name:
    Interleukin-32
      Short name=IL-32
Alternative name(s):
    Natural killer cells protein 4
    Tumor necrosis factor alpha-inducing factor

Gene names

Name:	IL32
Synonyms:	NK4, TAIF

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	234 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Cytokine that may play a role in innate and adaptive immune responses. It induces various cytokines such as TNFA/TNF-alpha and IL8. It activates typical cytokine signal pathways of NF-kappa-B and p38 MAPK. Ref.2
Subcellular location	Secreted. Ref.2
Tissue specificity	Selectively expressed in lymphocytes. Expression more prominent in immunce cells than in non-immune cells. Ref.2
Induction	Expression increased after activation of T-cells by mitogens or activation of NK cells by IL-2. Ref.1

Ontologies

Keywords
Cellular component	Secreted
Coding sequence diversity	Alternative splicing
Domain	Signal
Molecular function	Cytokine
Gene Ontology (GO)
Biological process	cell adhesion Ref.1 Traceable author statement. Source: ProtInc defense response Ref.1 Traceable author statement. Source: ProtInc immune response Ref.1 Traceable author statement. Source: ProtInc
Cellular component	extracellular space Ref.1 Traceable author statement. Source: ProtInc
Molecular function	cytokine activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: P24001-1)

Also known as: Gamma;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P24001-2)

Also known as: Beta;

The sequence of this isoform differs from the canonical sequence as follows:
     19-64: Missing.

Isoform 3 (identifier: P24001-3)

Also known as: Delta;

The sequence of this isoform differs from the canonical sequence as follows:
     1-10: Missing.
     19-64: Missing.

Isoform 4 (identifier: P24001-4)

Also known as: Alpha;

The sequence of this isoform differs from the canonical sequence as follows:
     19-64: Missing.
     154-210: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 30	30	Potential
	Chain	31 – 234	204	Interleukin-32		PRO_0000021487
Natural variations
	Alternative sequence	1 – 10	10	Missing in isoform 3.		VSP_022005
	Alternative sequence	19 – 64	46	Missing in isoform 2, isoform 3 and isoform 4.		VSP_022006
	Alternative sequence	154 – 210	57	Missing in isoform 4.		VSP_022007
	Natural variant	152	1	D → N Ref.1		VAR_005506

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (Gamma) [UniParc]. Last modified December 12, 2006. Version 3. Checksum: 5F339FE2D348A761	FASTA	234	26,676
	10 20 30 40 50 60 MCFPKVLSDD MKKLKARMVM LLPTSAQGLG AWVSACDTED TVGHLGPWRD KDPALWCQLC 70 80 90 100 110 120 LSSQHQAIER FYDKMQNAES GRGQVMSSLA ELEDDFKEGY LETVAAYYEE QHPELTPLLE 130 140 150 160 170 180 KERDGLRCRG NRSPVPDVED PATEEPGESF CDKVMRWFQA MLQRLQTWWH GVLAWVKEKV 190 200 210 220 230 VALVHAVQAL WKQFQSFCCS LSELFMSSFQ SYGAPRGDKE ELTPQKCSEP QSSK
	Isoform 2 (Beta). Checksum: 601340225A3BB1CD	FASTA	188	21,737
	10 20 30 40 50 60 MCFPKVLSDD MKKLKARMHQ AIERFYDKMQ NAESGRGQVM SSLAELEDDF KEGYLETVAA 70 80 90 100 110 120 YYEEQHPELT PLLEKERDGL RCRGNRSPVP DVEDPATEEP GESFCDKVMR WFQAMLQRLQ 130 140 150 160 170 180 TWWHGVLAWV KEKVVALVHA VQALWKQFQS FCCSLSELFM SSFQSYGAPR GDKEELTPQK CSEPQSSK
	Isoform 3 (Delta). Checksum: 0B04D770664399C4	FASTA	178	20,600
	10 20 30 40 50 60 MKKLKARMHQ AIERFYDKMQ NAESGRGQVM SSLAELEDDF KEGYLETVAA YYEEQHPELT 70 80 90 100 110 120 PLLEKERDGL RCRGNRSPVP DVEDPATEEP GESFCDKVMR WFQAMLQRLQ TWWHGVLAWV 130 140 150 160 170 KEKVVALVHA VQALWKQFQS FCCSLSELFM SSFQSYGAPR GDKEELTPQK CSEPQSSK
	Isoform 4 (Alpha). Checksum: A31708A3A25CFF6F	FASTA	131	14,909
	10 20 30 40 50 60 MCFPKVLSDD MKKLKARMHQ AIERFYDKMQ NAESGRGQVM SSLAELEDDF KEGYLETVAA 70 80 90 100 110 120 YYEEQHPELT PLLEKERDGL RCRGNRSPVP DVEDPATEEP GESFCDKSYG APRGDKEELT 130 PQKCSEPQSS K

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of a novel gene expressed in activated natural killer cells and T cells." Dahl C.A., Schall R.P., He H., Cairns J.S. J. Immunol. 148:597-603(1992) [PubMed: 1729377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), INDUCTION, VARIANT ASN-152.
[2]	"Interleukin-32: a cytokine and inducer of TNFalpha." Kim S.-H., Han S.-Y., Azam T., Yoon D.-Y., Dinarello C.A. Immunity 22:131-142(2005) [PubMed: 15664165] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORMS 2; 3 AND 4), ALTERNATIVE SPLICING, FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[3]	"The sequence and analysis of duplication-rich human chromosome 16." Martin J., Han C., Gordon L.A., Terry A., Prabhakar S., She X., Xie G., Hellsten U., Chan Y.M., Altherr M., Couronne O., Aerts A., Bajorek E., Black S., Blumer H., Branscomb E., Brown N.C., Bruno W.J. , Buckingham J.M., Callen D.F., Campbell C.S., Campbell M.L., Campbell E.W., Caoile C., Challacombe J.F., Chasteen L.A., Chertkov O., Chi H.C., Christensen M., Clark L.M., Cohn J.D., Denys M., Detter J.C., Dickson M., Dimitrijevic-Bussod M., Escobar J., Fawcett J.J., Flowers D., Fotopulos D., Glavina T., Gomez M., Gonzales E., Goodstein D., Goodwin L.A., Grady D.L., Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Hildebrand C.E., Huang W., Israni S., Jett J., Jewett P.B., Kadner K., Kimball H., Kobayashi A., Krawczyk M.-C., Leyba T., Longmire J.L., Lopez F., Lou Y., Lowry S., Ludeman T., Manohar C.F., Mark G.A., McMurray K.L., Meincke L.J., Morgan J., Moyzis R.K., Mundt M.O., Munk A.C., Nandkeshwar R.D., Pitluck S., Pollard M., Predki P., Parson-Quintana B., Ramirez L., Rash S., Retterer J., Ricke D.O., Robinson D.L., Rodriguez A., Salamov A., Saunders E.H., Scott D., Shough T., Stallings R.L., Stalvey M., Sutherland R.D., Tapia R., Tesmer J.G., Thayer N., Thompson L.S., Tice H., Torney D.C., Tran-Gyamfi M., Tsai M., Ulanovsky L.E., Ustaszewska A., Vo N., White P.S., Williams A.L., Wills P.L., Wu J.-R., Wu K., Yang J., DeJong P., Bruce D., Doggett N.A., Deaven L., Schmutz J., Grimwood J., Richardson P., Rokhsar D.S., Eichler E.E., Gilna P., Lucas S.M., Myers R.M., Rubin E.M., Pennacchio L.A. Nature 432:988-994(2004) [PubMed: 15616553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Eye and Pancreas.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M59807 mRNA. Translation: AAA36367.2. AY495331 mRNA. Translation: AAS80143.1. AY495332 mRNA. Translation: AAS80144.1. AY495333 mRNA. Translation: AAS80145.1. AY495334 Genomic DNA. Translation: AAS80146.1. AY495334 Genomic DNA. Translation: AAS80147.1. AY495334 Genomic DNA. Translation: AAS80148.1. AC108134 Genomic DNA. No translation available. BC009401 mRNA. Translation: AAH09401.1. BC018782 mRNA. Translation: AAH18782.1. BC105602 mRNA. Translation: AAI05603.1.
IPI	IPI00103009. IPI00386710. IPI00478532. IPI00816727.
PIR	I56140.
RefSeq	NP_001012649.1. NP_001012651.1. NP_001012736.1. NP_004212.4.
UniGene	Hs.943
3D structure databases
ModBase	Search...
PTM databases
PhosphoSite	P24001.
Genome annotation databases
Ensembl	ENSG00000008517. Homo sapiens. [Contig view]
GeneID	9235.
KEGG	hsa:9235.
NMPDR	fig|9606.3.peg.11613.
Organism-specific databases
GeneCards	GC16P003056.
HGNC	HGNC:16830. IL32.
MIM	606001. gene.
PharmGKB	PA128395754.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	P24001.
HOVERGEN	P24001.
OMA	P24001. HQAIERF.
Gene expression databases
ArrayExpress	P24001.
Bgee	P24001.
CleanEx	HS_IL32.
GermOnline	ENSG00000008517. Homo sapiens.
Family and domain databases
ProtoNet	Search...
Other Resources
NextBio	34609.
SOURCE	Search...

Entry information

Entry name

IL32_HUMAN

Accession

Primary (citable) accession number: P24001
Secondary accession number(s): Q5VFH7 , Q5VFH8, Q8WV38, Q96GK9

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 1992
Last sequence update:	December 12, 2006
Last modified:	June 16, 2009
This is version 58 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)