P24000 (RL24B_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
60S ribosomal protein L24-B

Alternative name(s):
L30
RP29
YL21

Gene names

Name:	RPL24B
Synonyms:	RPL30B
Ordered Locus Names:	YGR148C
ORF Names:	G6635

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	155 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Subunit structure	Component of the large ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S). Ref.7
Subcellular location	Cytoplasm Ref.8.
Miscellaneous	Present with 36900 molecules/cell in log phase SD medium. There are 2 genes for L24 in yeast.
Sequence similarities	Belongs to the ribosomal protein L24e family.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Ribonucleoprotein Ribosomal protein
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cytoplasmic translation Traceable author statement Ref.7. Source: SGD
Cellular_component	cytosolic large ribosomal subunit Traceable author statement Ref.7. Source: SGD
Molecular_function	RNA binding Inferred from direct assay PubMed 6337137. Source: SGD structural constituent of ribosome Traceable author statement Ref.7. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 155	155	60S ribosomal protein L24-B		PRO_0000136895
Amino acid modifications
	Modified residue	7	1	Phosphoserine Ref.11 Ref.12
	Modified residue	9	1	Phosphoserine Ref.12
	Modified residue	86	1	Phosphoserine Ref.10

Sequences

	Sequence		Length	Mass (Da)
	P24000 [UniParc]. Last modified March 1, 1992. Version 1. Checksum: 7EE89CE40D7A657F	FASTA	155	17,548
	10 20 30 40 50 60 MKVEVDSFSG AKIYPGRGTL FVRGDSKIFR FQNSKSASLF KQRKNPRRIA WTVLFRKHHK 70 80 90 100 110 120 KGITEEVAKK RSRKTVKAQR PITGASLDLI KERRSLKPEV RKANREEKLK ANKEKKRAEK 130 140 150 AARKAEKAKS AGVQGSKVSK QQAKGAFQKV AATSR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Ribosomal protein L30 is dispensable in the yeast Saccharomyces cerevisiae." Baronas-Lowell D.M., Warner J.R. Mol. Cell. Biol. 10:5235-5243(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The sequence of a 27 kb segment on the right arm of chromosome VII from Saccharomyces cerevisiae reveals MOL1, NAT2, RPL30B, RSR1, CYS4, PEM1/CHO2, NSR1 genes and ten new open reading frames." Skala J., Nawrocki A., Goffeau A. Yeast 11:1421-1427(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K. Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[6]	"High-density yeast-tiling array reveals previously undiscovered introns and extensive regulation of meiotic splicing." Juneau K., Palm C., Miranda M., Davis R.W. Proc. Natl. Acad. Sci. U.S.A. 104:1522-1527(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-86. Strain: ATCC 201390 / BY4743.
[7]	"The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae." Planta R.J., Mager W.H. Yeast 14:471-477(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NOMENCLATURE, SUBUNIT.
[8]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[9]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[10]	"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway." Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N. Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-86, MASS SPECTROMETRY. Strain: YAL6B.
[11]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-7, MASS SPECTROMETRY.
[12]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-7 AND SER-9, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M34387 Genomic DNA. Translation: AAA34736.1. X85807 Genomic DNA. Translation: CAA59806.1. Z72933 Genomic DNA. Translation: CAA97162.1. AY557819 Genomic DNA. Translation: AAS56145.1. EF123136 mRNA. Translation: ABM97480.1. BK006941 Genomic DNA. Translation: DAA08240.1.
PIR	R6BYY0. A35925.
RefSeq	NP_011664.3. NM_001181277.3.
3D structure databases
ProteinModelPortal	P24000.
SMR	P24000. Positions 1-134.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-4722N.
IntAct	P24000. 4 interactions.
MINT	MINT-502170.
Proteomic databases
PaxDb	P24000.
PeptideAtlas	P24000.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YGR148C; YGR148C; YGR148C.
GeneID	853051.
KEGG	sce:YGR148C. sce:YGR155W.
Organism-specific databases
CYGD	YGR148c.
SGD	S000003380. RPL24B.
Phylogenomic databases
eggNOG	COG2075.
GeneTree	ENSGT00550000074809.
HOGENOM	HOG000184343.
KO	K01697. K02896.
OMA	IHPGHGK.
OrthoDB	EOG4W11PR.
Gene expression databases
Genevestigator	P24000.
GermOnline	YGR148C. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	2.30.170.20. 1 hit.
InterPro	IPR023442. Ribosomal_L24e_CS. IPR023441. Ribosomal_L24e_dom. IPR000988. Ribosomal_L24e_rel. [Graphical view]
PANTHER	PTHR10792. PTHR10792. 1 hit.
Pfam	PF01246. Ribosomal_L24e. 1 hit. [Graphical view]
PROSITE	PS01073. RIBOSOMAL_L24E. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	972972.

Entry information

Entry name

RL24B_YEAST

Accession

Primary (citable) accession number: P24000
Secondary accession number(s): A2TBN3, D6VUS9

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 1992
Last sequence update:	March 1, 1992
Last modified:	April 3, 2013
This is version 110 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program