P23991 (ADH1_CHICK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alcohol dehydrogenase 1
Short name=ADH-1
EC=1.1.1.1

Alternative name(s):
Alcohol dehydrogenase I

Gene names

Name:

ADH1

Organism

Gallus gallus (Chicken)

Taxonomic identifier

9031 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galliformes › Phasianidae › Phasianinae › Gallus

Protein attributes

Sequence length	376 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	An alcohol + NAD+ = an aldehyde or ketone + NADH.
Cofactor	Binds 2 zinc ions per subunit.
Subunit structure	Homodimer.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the zinc-containing alcohol dehydrogenase family. Class-I subfamily.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Metal-binding NAD Zinc
Molecular function	Oxidoreductase
PTM	Acetylation
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	alcohol dehydrogenase (NAD) activity Inferred from electronic annotation. Source: EC nucleotide binding Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.2
	Chain	2 – 376	375	Alcohol dehydrogenase 1		PRO_0000160672
Regions
	Nucleotide binding	201 – 206	6	NAD By similarity
	Nucleotide binding	294 – 296	3	NAD By similarity
Sites
	Metal binding	47	1	Zinc 1; catalytic
	Metal binding	68	1	Zinc 1; catalytic
	Metal binding	98	1	Zinc 2
	Metal binding	101	1	Zinc 2
	Metal binding	104	1	Zinc 2
	Metal binding	112	1	Zinc 2
	Metal binding	176	1	Zinc 1; catalytic
	Binding site	225	1	NAD By similarity
	Binding site	230	1	NAD By similarity
	Binding site	371	1	NAD By similarity
Amino acid modifications
	Modified residue	2	1	N-acetylserine Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P23991 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: CCD03989E53273DE	FASTA	376	39,808
	10 20 30 40 50 60 MSTVGKVIKC KAAVLWEANK PFSLEEVEVA PPKAHEVRIK IVATGICRSD DHVVTGALAM 70 80 90 100 110 120 PFPIILGHEA AGVIESVGEK VTSLKPGDAV IPLFVPQCGE CRSCLSTKGN LCIKNDLSSS 130 140 150 160 170 180 PTGLMADGTT RFTCKGKAIH HFVGTSTFTE YTVVHETAAA KIDSAAPLEK VCLIGCGFST 190 200 210 220 230 240 GYGAVLQTAK VEAGSTCAVF GLGGVGLSVV MGCKAAGASR IIAVDINKDK FAKAKELGAT 250 260 270 280 290 300 ECINPKDFKK PIHEVLTEMT GQGVDYSFEV IGRIETMTAA LASCHNNYGV SVIVGVPPAA 310 320 330 340 350 360 QKISFDPMLI FSGRTWKGSV FGGWKSKDAV PKLVADYMKK KFVLDPLITH TLPFTKINEG 370 FDLLRTGKSI RSVLVL

References

[1]	"Avian alcohol dehydrogenase: the chicken liver enzyme. Primary structure, cDNA-cloning, and relationships to other alcohol dehydrogenases." Estonius M., Karlsson C., Fox E.A., Hoeoeg J.-O., Holmquist B., Vallee B.L., Davidson W.S., Joernvall H. Eur. J. Biochem. 194:593-602(1990) [PubMed: 2269284] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Liver.
[2]	"Fast atom bombardment mass spectrometry and chemical analysis in determinations of acyl-blocked protein structures." Egestad B., Estonius M., Danielsson O., Persson B., Cederlund E., Kaiser R., Holmquist B., Vallee B., Pares X., Jefferey J., Joernvall H. FEBS Lett. 269:194-196(1990) [PubMed: 2387402] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-17 AND 360-376.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X54612 mRNA. Translation: CAA38433.1. Sequence problems.
IPI	IPI00599701.
PIR	DECHA1. S13851.
UniGene	Gga.4115.
3D structure databases
ProteinModelPortal	P23991.
SMR	P23991. Positions 2-376.
ModBase	Search...
Protein-protein interaction databases
STRING	P23991.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
eggNOG	veNOG15402.
GeneTree	ENSGT00430000030800.
HOGENOM	HBG753318.
HOVERGEN	HBG000195.
InParanoid	P23991.
OrthoDB	EOG4BRWM5.
PhylomeDB	P23991.
Family and domain databases
InterPro	IPR013149. ADH_C. IPR013154. ADH_GroES-like. IPR002085. ADH_SF_Zn-type. IPR002328. ADH_Zn_CS. IPR011032. GroES-like. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR11695. ADH_Sf_Zn. 1 hit.
Pfam	PF08240. ADH_N. 1 hit. PF00107. ADH_zinc_N. 1 hit. [Graphical view]
SUPFAM	SSF50129. GroES_like. 2 hits.
PROSITE	PS00059. ADH_ZINC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ADH1_CHICK

Accession

Primary (citable) accession number: P23991

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 1992
Last sequence update:	January 23, 2007
Last modified:	December 14, 2011
This is version 82 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program