Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Beta-galactosidase

Gene

lacZ

Organism
Streptococcus thermophilus
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei458 – 4581Proton donorBy similarity
Active sitei546 – 5461NucleophileBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Enzyme and pathway databases

SABIO-RKP23989.

Protein family/group databases

CAZyiGH2. Glycoside Hydrolase Family 2.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-galactosidase (EC:3.2.1.23)
Short name:
Beta-gal
Alternative name(s):
Lactase
Gene namesi
Name:lacZ
OrganismiStreptococcus thermophilus
Taxonomic identifieri1308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10261026Beta-galactosidasePRO_0000057676Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi264199.stu1397.

Structurei

3D structure databases

ProteinModelPortaliP23989.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 2 family.Curated

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF02929. Bgal_small_N. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSiPR00132. GLHYDRLASE2.
SMARTiSM01038. Bgal_small_N. 1 hit.
[Graphical view]
SUPFAMiSSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEiPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P23989-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNMTEKIQTY LNDPKIVSVN TVDAHSDHKY FESLEEFSEG EMKLRQSLNG
60 70 80 90 100
KWKIHYAQNT NQVLKDFYKT EFDETDLNFI NVPGHLELQG FGSPQYVNTQ
110 120 130 140 150
YPWDGKEFLR PPQVPQESNA VASYVKHFTL NDALKDKKVF ISFQGVATSI
160 170 180 190 200
FVWVNGNFVG YSEDSFTPSE FEISDYLVEG DNKLAVAVYR YSTASWLEDQ
210 220 230 240 250
DFWRLYGIFR DVYLYAIPKV HVQDLFVKGD YDYQTKAGQL DIDLKTVGDY
260 270 280 290 300
EDKKIKYVLS DYEGIVTEGD ASVNGDGELS VSLENLKIKP WSAESPKLYD
310 320 330 340 350
LILHVLDDDQ VVEVVPVKVG FRRFEIKDKL MLLNGKRIVF KGVNRHEFNA
360 370 380 390 400
RTGRCITEED MLWDIKVMKQ HNINAVRTSH YPNQTRWYEL CDEYGLYVID
410 420 430 440 450
EANLETHGTW QKLGLCEPSW NIPASEPEWL PACLDRANNM FQRDKNHASV
460 470 480 490 500
IIWSCGNESY AGKDIADMAD YFRSVDNTRP VHYEGVAWCR EFDYITDIES
510 520 530 540 550
RMYAKPADIE EYLTTGKLVD LSSVSDKHFA SGNLTNKPQK PYISCEYMHT
560 570 580 590 600
MGNSGGGLQL YTDLEKYPEY QGGFIWDFID QAIYKTLPNG SEFLSYGGDW
610 620 630 640 650
HDRPSDYEFC GNGIVFADRT LTPKLQTVKH LYSNIKIAVD EKSVTIKNDN
660 670 680 690 700
LFEDLSAYTF LARVYEDGRK VSESEYHFDV KPGEEATFPV NFVVEASNSE
710 720 730 740 750
QIYEVACVLR EATEWAPKGH EIVRGQYVVE KISTETPVKA PLNVVEGDFN
760 770 780 790 800
IGIQGQNFSI LLSRAQNTLV SAKYNGVEFI EKGPKLSFTR AYTDNDRGAG
810 820 830 840 850
YPFEMAGWKV AGNYSKVTDT QIQIEDDSVK VTYVHELPGL SDVEVKVTYQ
860 870 880 890 900
VDYKGRIFVT ANYDGKAGLP NFPEFGLEFA IGSQFTNLSY YGYGAEESYR
910 920 930 940 950
DKLPGAYLGR YETSVEKTFA PYLMPQESGN HYGTREFTVS DDNHNGLKFT
960 970 980 990 1000
ALNKAFEFSA LRNSTEQIEN ARHQYELQES DATWIKVLAA QMGVGGDDTW
1010 1020
GAPVHDEFLL SSADSYQLSF MIEPLN
Length:1,026
Mass (Da):117,046
Last modified:March 1, 1992 - v1
Checksum:iB453AFAD68F08C98
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M63636 Genomic DNA. Translation: AAA63532.1.
M63636 Genomic DNA. Translation: AAA63533.1.
M23009 Genomic DNA. Translation: AAA26911.1.
PIRiA49750.
RefSeqiWP_011226267.1. NZ_CP011217.1.

Genome annotation databases

GeneIDi3164581.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M63636 Genomic DNA. Translation: AAA63532.1.
M63636 Genomic DNA. Translation: AAA63533.1.
M23009 Genomic DNA. Translation: AAA26911.1.
PIRiA49750.
RefSeqiWP_011226267.1. NZ_CP011217.1.

3D structure databases

ProteinModelPortaliP23989.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi264199.stu1397.

Protein family/group databases

CAZyiGH2. Glycoside Hydrolase Family 2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi3164581.

Enzyme and pathway databases

SABIO-RKP23989.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF02929. Bgal_small_N. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSiPR00132. GLHYDRLASE2.
SMARTiSM01038. Bgal_small_N. 1 hit.
[Graphical view]
SUPFAMiSSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEiPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Analysis of the lacZ sequences from two Streptococcus thermophilus strains: comparison with the Escherichia coli and Lactobacillus bulgaricus beta-galactosidase sequences."
    Schroeder C.J., Robert C., Lenzen G., McKay L.L., Mercenier A.
    J. Gen. Microbiol. 137:369-380(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: A054.
  2. "Lactose transport system of Streptococcus thermophilus: a hybrid protein with homology to the melibiose carrier and enzyme III of phosphoenolpyruvate-dependent phosphotransferase systems."
    Poolman B., Royer T.J., Mainzer S.E., Schmidt B.F.
    J. Bacteriol. 171:244-253(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-108.

Entry informationi

Entry nameiBGAL_STRTR
AccessioniPrimary (citable) accession number: P23989
Secondary accession number(s): Q6LDR7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: March 1, 1992
Last modified: July 22, 2015
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.