P23981 (AROA1_TOBAC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-phosphoshikimate 1-carboxyvinyltransferase 1, chloroplastic
EC=2.5.1.19

Alternative name(s):
5-enolpyruvylshikimate-3-phosphate synthase 1
Short name=EPSP synthase 1

Gene names

Name:

EPSPS-1

Organism

Nicotiana tabacum (Common tobacco)

Taxonomic identifier

4097 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Nicotianoideae › Nicotianeae › Nicotiana

Protein attributes

Sequence length	518 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	Phosphoenolpyruvate + 3-phosphoshikimate = phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate.
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 6/7.
Subcellular location	Plastid › chloroplast.
Miscellaneous	This enzyme is the target of the potent, broad-spectrum herbicide, glyphosate [n-(phosphonomethyl)glycine]. Overproduction of EPSP leads to glyphosate tolerance.
Sequence similarities	Belongs to the EPSP synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Molecular function	Transferase
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-phosphoshikimate 1-carboxyvinyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 74	74	Chloroplast
	Chain	75 – 518	444	3-phosphoshikimate 1-carboxyvinyltransferase 1, chloroplastic		PRO_0000002291

Sequences

	Sequence		Length	Mass (Da)
	P23981 [UniParc]. Last modified March 1, 1992. Version 1. Checksum: 4F03C514EAFE1681	FASTA	518	55,711
	10 20 30 40 50 60 MAQISSMGQG IRTPNLNSYL PKTQKVPLFS HSIFIGSKKI TQNSAKSLWV SKEDSVLRVA 70 80 90 100 110 120 KSPFRISASV VTAQKPNEIV LQPIKDISGT VKLPGSKSLS NRILLLAALS KGRTVVDNLL 130 140 150 160 170 180 SSDDIHYMLG ALKTLGLHVE DDNENQRAIV EGCGGQFPVG KKSEEEIQLF LGNAGTAMRP 190 200 210 220 230 240 LTAAVTVAGG HSRYVLDGVP RMRERPIGDL VDGLKQLGAE VDCFLGTNCP PVRIVSKGGL 250 260 270 280 290 300 PGGKVKLSGS ISSQYLTALL MAAPLALGDV EIEIIDKLIS VPYVEMTLKL MERFGVSVEH 310 320 330 340 350 360 TSSWDKFLVR GGQKYKSPGK AYVEGDASSA SYFLAGAAVT GGTVTVEGCG TSSLQGDVKF 370 380 390 400 410 420 AEVLEKMGAE VTWTENSVTV KGPPRNSSGM KHLRAVDVNM NKMPDVAMTL AVVALFADGP 430 440 450 460 470 480 TAIRDVASWR VKETERMIAI CTELRKLGAT VVEGSDYCII TPPEKLNVTE IDTYDDHRMA 490 500 510 MAFSLAACAD VPVTIKDPGC TRKTFPNYFD VLQQYSKH

References

[1]	"Expression and stability of amplified genes encoding 5-enolpyruvylshikimate-3-phosphate synthase in glyphosate-tolerant tobacco cells." Wang Y., Jones J., Weller S., Goldsbrough P.B. Plant Mol. Biol. 17:1127-1138(1991) [PubMed: 1932690] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M61904 mRNA. Translation: AAA34071.1.
PIR	S18353.
3D structure databases
ProteinModelPortal	P23981.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR001986. Enolpyruvate_Tfrase_dom. IPR006264. EPSP_synthase. IPR023193. EPSP_synthase_CS. IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b. [Graphical view]
Gene3D	G3DSA:3.65.10.10. EPSP_synthase. 2 hits.
Pfam	PF00275. EPSP_synthase. 1 hit. [Graphical view]
SUPFAM	SSF55205. RNA3'_cycl/enolpyr_transf_A/B. 1 hit.
TIGRFAMs	TIGR01356. AroA. 1 hit.
PROSITE	PS00104. EPSP_SYNTHASE_1. 1 hit. PS00885. EPSP_SYNTHASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AROA1_TOBAC

Accession

Primary (citable) accession number: P23981

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 1992
Last sequence update:	March 1, 1992
Last modified:	September 21, 2011
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program