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P23979

- 5HT3A_MOUSE

UniProt

P23979 - 5HT3A_MOUSE

Protein

5-hydroxytryptamine receptor 3A

Gene

Htr3a

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 128 (01 Oct 2014)
      Sequence version 1 (01 Mar 1992)
      Previous versions | rss
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    Functioni

    This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. This receptor is a ligand-gated ion channel, which when activated causes fast, depolarizing responses in neurons. It is a cation-specific, but otherwise relatively nonselective, ion channel.1 Publication

    GO - Molecular functioni

    1. extracellular ligand-gated ion channel activity Source: InterPro
    2. serotonin binding Source: MGI
    3. serotonin receptor activity Source: MGI

    GO - Biological processi

    1. serotonin receptor signaling pathway Source: GOC

    Keywords - Molecular functioni

    Ion channel, Ligand-gated ion channel, Receptor

    Keywords - Biological processi

    Ion transport, Transport

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    5-hydroxytryptamine receptor 3A
    Short name:
    5-HT3-A
    Short name:
    5-HT3A
    Alternative name(s):
    5-hydroxytryptamine receptor 3
    Short name:
    5-HT-3
    Short name:
    5-HT3R
    Serotonin receptor 3A
    Serotonin-gated ion channel receptor
    Gene namesi
    Name:Htr3a
    Synonyms:5ht3, Htr3
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Unplaced

    Organism-specific databases

    MGIiMGI:96282. Htr3a.

    Subcellular locationi

    GO - Cellular componenti

    1. cell junction Source: UniProtKB-KW
    2. integral component of membrane Source: UniProtKB-KW
    3. postsynaptic membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2323Sequence AnalysisAdd
    BLAST
    Chaini24 – 4874645-hydroxytryptamine receptor 3APRO_0000000409Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi108 – 1081N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi161 ↔ 175By similarity
    Glycosylationi174 – 1741N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi190 – 1901N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PRIDEiP23979.

    PTM databases

    PhosphoSiteiP23979.

    Expressioni

    Tissue specificityi

    Brain, spinal cord, and heart.

    Gene expression databases

    CleanExiMM_HTR3A.
    GenevestigatoriP23979.

    Interactioni

    Subunit structurei

    Forms pentahomomeric complex as well as pentaheteromeric complex with HTR3B; homomeric complex is functional but exhibits low conductance with modified voltage dependence, and decreased agonist and antagonist affinity. Interacts with RIC3 By similarity.By similarity

    Protein-protein interaction databases

    DIPiDIP-48769N.
    STRINGi10090.ENSMUSP00000003826.

    Structurei

    3D structure databases

    ProteinModelPortaliP23979.
    SMRiP23979. Positions 45-335.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini24 – 245222ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini273 – 2775CytoplasmicSequence Analysis
    Topological domaini297 – 3059ExtracellularSequence Analysis
    Topological domaini325 – 464140CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini485 – 4873ExtracellularSequence Analysis

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei246 – 27227Helical; Name=1Sequence AnalysisAdd
    BLAST
    Transmembranei278 – 29619Helical; Name=2Sequence AnalysisAdd
    BLAST
    Transmembranei306 – 32419Helical; Name=3Sequence AnalysisAdd
    BLAST
    Transmembranei465 – 48420Helical; Name=4Sequence AnalysisAdd
    BLAST

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni423 – 45937HA-stretchAdd
    BLAST

    Sequence similaritiesi

    Keywords - Domaini

    Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG302526.
    HOGENOMiHOG000241519.
    HOVERGENiHBG106638.
    InParanoidiP23979.
    KOiK04819.
    PhylomeDBiP23979.

    Family and domain databases

    Gene3Di1.20.120.370. 2 hits.
    2.70.170.10. 1 hit.
    InterProiIPR008132. 5HT3_rcpt.
    IPR008133. 5HT3_rcpt_A.
    IPR027361. Acetylcholine_rcpt_TM.
    IPR006202. Neur_chan_lig-bd.
    IPR006201. Neur_channel.
    IPR006029. Neurotrans-gated_channel_TM.
    IPR018000. Neurotransmitter_ion_chnl_CS.
    [Graphical view]
    PANTHERiPTHR18945. PTHR18945. 1 hit.
    PfamiPF02931. Neur_chan_LBD. 1 hit.
    PF02932. Neur_chan_memb. 1 hit.
    [Graphical view]
    PRINTSiPR01709. 5HT3ARECEPTR.
    PR01708. 5HT3RECEPTOR.
    PR00252. NRIONCHANNEL.
    SUPFAMiSSF63712. SSF63712. 1 hit.
    SSF90112. SSF90112. 1 hit.
    TIGRFAMsiTIGR00860. LIC. 1 hit.
    PROSITEiPS00236. NEUROTR_ION_CHANNEL. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 5-HT3R-A (identifier: P23979-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MRLCIPQVLL ALFLSMLTAP GEGSRRRATQ EDTTQPALLR LSDHLLANYK    50
    KGVRPVRDWR KPTTVSIDVI MYAILNVDEK NQVLTTYIWY RQYWTDEFLQ 100
    WTPEDFDNVT KLSIPTDSIW VPDILINEFV DVGKSPNIPY VYVHHRGEVQ 150
    NYKPLQLVTA CSLDIYNFPF DVQNCSLTFT SWLHTIQDIN ITLWRSPEEV 200
    RSDKSIFINQ GEWELLEVFP QFKEFSIDIS NSYAEMKFYV IIRRRPLFYA 250
    VSLLLPSIFL MVVDIVGFCL PPDSGERVSF KITLLLGYSV FLIIVSDTLP 300
    ATIGTPLIGV YFVVCMALLV ISLAETIFIV RLVHKQDLQR PVPDWLRHLV 350
    LDRIAWILCL GEQPMAHRPP ATFQANKTDD CSGSDLLPAM GNHCSHVGGP 400
    QDLEKTPRGR GSPLPPPREA SLAVRGLLQE LSSIRHFLEK RDEMREVARD 450
    WLRVGYVLDR LLFRIYLLAV LAYSITLVTL WSIWHYS 487
    Length:487
    Mass (Da):56,056
    Last modified:March 1, 1992 - v1
    Checksum:iD0148867C8536D66
    GO
    Isoform 5-HT3R-AS (identifier: P23979-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         383-388: Missing.

    Show »
    Length:481
    Mass (Da):55,474
    Checksum:i7A9B7B5C38FEC909
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti31 – 311E → AR in CAA51089. (PubMed:7683998)Curated
    Sequence conflicti31 – 311E → AR in CAA55870. (PubMed:7854052)Curated
    Sequence conflicti31 – 311E → AR in CAA55871. (PubMed:7854052)Curated
    Sequence conflicti74 – 741I → V in CAA51089. (PubMed:7683998)Curated
    Sequence conflicti302 – 3021T → TA in CAA51089. (PubMed:7683998)Curated
    Sequence conflicti302 – 3021T → TA in CAA55870. (PubMed:7854052)Curated
    Sequence conflicti302 – 3021T → TA in CAA55871. (PubMed:7854052)Curated
    Sequence conflicti384 – 3841S → SS in CAA80453. (PubMed:8112471)Curated
    Sequence conflicti393 – 3931H → T in CAA55870. (PubMed:7854052)Curated
    Sequence conflicti393 – 3931H → T in CAA55871. (PubMed:7854052)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei383 – 3886Missing in isoform 5-HT3R-AS. CuratedVSP_000079

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M74425 mRNA. Translation: AAA37124.1.
    Z22772, Z22773 Genomic DNA. Translation: CAA80453.1.
    X72395 mRNA. Translation: CAA51089.1.
    X79283 Genomic DNA. Translation: CAA55870.1.
    X79283 Genomic DNA. Translation: CAA55871.1.
    PIRiS41757.
    RefSeqiNP_038589.2. NM_013561.2.
    UniGeneiMm.4831.

    Genome annotation databases

    GeneIDi15561.
    KEGGimmu:15561.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M74425 mRNA. Translation: AAA37124.1 .
    Z22772 , Z22773 Genomic DNA. Translation: CAA80453.1 .
    X72395 mRNA. Translation: CAA51089.1 .
    X79283 Genomic DNA. Translation: CAA55870.1 .
    X79283 Genomic DNA. Translation: CAA55871.1 .
    PIRi S41757.
    RefSeqi NP_038589.2. NM_013561.2.
    UniGenei Mm.4831.

    3D structure databases

    ProteinModelPortali P23979.
    SMRi P23979. Positions 45-335.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-48769N.
    STRINGi 10090.ENSMUSP00000003826.

    Chemistry

    BindingDBi P23979.
    ChEMBLi CHEMBL4972.
    GuidetoPHARMACOLOGYi 373.

    PTM databases

    PhosphoSitei P23979.

    Proteomic databases

    PRIDEi P23979.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 15561.
    KEGGi mmu:15561.

    Organism-specific databases

    CTDi 3359.
    MGIi MGI:96282. Htr3a.

    Phylogenomic databases

    eggNOGi NOG302526.
    HOGENOMi HOG000241519.
    HOVERGENi HBG106638.
    InParanoidi P23979.
    KOi K04819.
    PhylomeDBi P23979.

    Miscellaneous databases

    NextBioi 288512.
    PROi P23979.
    SOURCEi Search...

    Gene expression databases

    CleanExi MM_HTR3A.
    Genevestigatori P23979.

    Family and domain databases

    Gene3Di 1.20.120.370. 2 hits.
    2.70.170.10. 1 hit.
    InterProi IPR008132. 5HT3_rcpt.
    IPR008133. 5HT3_rcpt_A.
    IPR027361. Acetylcholine_rcpt_TM.
    IPR006202. Neur_chan_lig-bd.
    IPR006201. Neur_channel.
    IPR006029. Neurotrans-gated_channel_TM.
    IPR018000. Neurotransmitter_ion_chnl_CS.
    [Graphical view ]
    PANTHERi PTHR18945. PTHR18945. 1 hit.
    Pfami PF02931. Neur_chan_LBD. 1 hit.
    PF02932. Neur_chan_memb. 1 hit.
    [Graphical view ]
    PRINTSi PR01709. 5HT3ARECEPTR.
    PR01708. 5HT3RECEPTOR.
    PR00252. NRIONCHANNEL.
    SUPFAMi SSF63712. SSF63712. 1 hit.
    SSF90112. SSF90112. 1 hit.
    TIGRFAMsi TIGR00860. LIC. 1 hit.
    PROSITEi PS00236. NEUROTR_ION_CHANNEL. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Primary structure and functional expression of the 5HT3 receptor, a serotonin-gated ion channel."
      Maricq A.V., Peterson A.S., Brake A.J., Myers R.M., Julius D.
      Science 254:432-437(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Organisation of the murine 5-HT3 receptor gene and assignment to human chromosome 11."
      Uetz P., Abdelatty F., Villarroel A., Gundrun R., Weiss B., Koenen M.
      FEBS Lett. 339:302-306(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: BALB/c.
      Tissue: Brain.
    3. "Cloning and functional expression of an apparent splice variant of the murine 5-HT3 receptor A subunit."
      Hope A.G., Downie D.L., Sutherland L., Lambert J.J., Peters J.A., Burchell B.
      Eur. J. Pharmacol. 245:187-192(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: A/J.
    4. "Organization of the mouse 5-HT3 receptor gene and functional expression of two splice variants."
      Werner P., Kawashima E., Reid J., Hussy N., Lundstrom K., Buell G., Humbert Y., Jones K.A.
      Brain Res. Mol. Brain Res. 26:233-241(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], ALTERNATIVE SPLICING.
      Strain: 129/Sv.
    5. "A cytoplasmic region determines single-channel conductance in 5-HT3 receptors."
      Kelley S.P., Dunlop J.I., Kirkness E.F., Lambert J.J., Peters J.A.
      Nature 424:321-324(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, REGION.

    Entry informationi

    Entry namei5HT3A_MOUSE
    AccessioniPrimary (citable) accession number: P23979
    Secondary accession number(s): Q61225, Q61226
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 1, 1992
    Last sequence update: March 1, 1992
    Last modified: October 1, 2014
    This is version 128 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    The HA-stretch region of HTR3A seems to be responsible for the low conductance of HTR3A homomers compared to that of HTR3A/HTR3B heteromers.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3