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P23979

- 5HT3A_MOUSE

UniProt

P23979 - 5HT3A_MOUSE

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Protein

5-hydroxytryptamine receptor 3A

Gene

Htr3a

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. This receptor is a ligand-gated ion channel, which when activated causes fast, depolarizing responses in neurons. It is a cation-specific, but otherwise relatively nonselective, ion channel.1 Publication

GO - Molecular functioni

  1. extracellular ligand-gated ion channel activity Source: InterPro
  2. serotonin binding Source: MGI
  3. serotonin receptor activity Source: MGI

GO - Biological processi

  1. serotonin receptor signaling pathway Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Ligand-gated ion channel, Receptor

Keywords - Biological processi

Ion transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
5-hydroxytryptamine receptor 3A
Short name:
5-HT3-A
Short name:
5-HT3A
Alternative name(s):
5-hydroxytryptamine receptor 3
Short name:
5-HT-3
Short name:
5-HT3R
Serotonin receptor 3A
Serotonin-gated ion channel receptor
Gene namesi
Name:Htr3a
Synonyms:5ht3, Htr3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Unplaced

Organism-specific databases

MGIiMGI:96282. Htr3a.

Subcellular locationi

GO - Cellular componenti

  1. cell junction Source: UniProtKB-KW
  2. integral component of membrane Source: UniProtKB-KW
  3. postsynaptic membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2323Sequence AnalysisAdd
BLAST
Chaini24 – 4874645-hydroxytryptamine receptor 3APRO_0000000409Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi108 – 1081N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi161 ↔ 175By similarity
Glycosylationi174 – 1741N-linked (GlcNAc...)Sequence Analysis
Glycosylationi190 – 1901N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiP23979.
PRIDEiP23979.

PTM databases

PhosphoSiteiP23979.

Expressioni

Tissue specificityi

Brain, spinal cord, and heart.

Gene expression databases

CleanExiMM_HTR3A.
GenevestigatoriP23979.

Interactioni

Subunit structurei

Forms pentahomomeric complex as well as pentaheteromeric complex with HTR3B; homomeric complex is functional but exhibits low conductance with modified voltage dependence, and decreased agonist and antagonist affinity. Interacts with RIC3 (By similarity).By similarity

Protein-protein interaction databases

DIPiDIP-48769N.
STRINGi10090.ENSMUSP00000003826.

Structurei

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4PIRX-ray3.50A/B/C/D/E32-485[»]
ProteinModelPortaliP23979.
SMRiP23979. Positions 45-335.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini24 – 245222ExtracellularSequence AnalysisAdd
BLAST
Topological domaini273 – 2775CytoplasmicSequence Analysis
Topological domaini297 – 3059ExtracellularSequence Analysis
Topological domaini325 – 464140CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini485 – 4873ExtracellularSequence Analysis

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei246 – 27227Helical; Name=1Sequence AnalysisAdd
BLAST
Transmembranei278 – 29619Helical; Name=2Sequence AnalysisAdd
BLAST
Transmembranei306 – 32419Helical; Name=3Sequence AnalysisAdd
BLAST
Transmembranei465 – 48420Helical; Name=4Sequence AnalysisAdd
BLAST

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni423 – 45937HA-stretchAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG302526.
HOGENOMiHOG000241519.
HOVERGENiHBG106638.
InParanoidiP23979.
KOiK04819.
PhylomeDBiP23979.

Family and domain databases

Gene3Di1.20.120.370. 2 hits.
2.70.170.10. 1 hit.
InterProiIPR008132. 5HT3_rcpt.
IPR008133. 5HT3_rcpt_A.
IPR027361. Acetylcholine_rcpt_TM.
IPR006202. Neur_chan_lig-bd.
IPR006201. Neur_channel.
IPR006029. Neurotrans-gated_channel_TM.
IPR018000. Neurotransmitter_ion_chnl_CS.
[Graphical view]
PANTHERiPTHR18945. PTHR18945. 1 hit.
PfamiPF02931. Neur_chan_LBD. 1 hit.
PF02932. Neur_chan_memb. 1 hit.
[Graphical view]
PRINTSiPR01709. 5HT3ARECEPTR.
PR01708. 5HT3RECEPTOR.
PR00252. NRIONCHANNEL.
SUPFAMiSSF63712. SSF63712. 1 hit.
SSF90112. SSF90112. 1 hit.
TIGRFAMsiTIGR00860. LIC. 1 hit.
PROSITEiPS00236. NEUROTR_ION_CHANNEL. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 5-HT3R-A (identifier: P23979-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRLCIPQVLL ALFLSMLTAP GEGSRRRATQ EDTTQPALLR LSDHLLANYK
60 70 80 90 100
KGVRPVRDWR KPTTVSIDVI MYAILNVDEK NQVLTTYIWY RQYWTDEFLQ
110 120 130 140 150
WTPEDFDNVT KLSIPTDSIW VPDILINEFV DVGKSPNIPY VYVHHRGEVQ
160 170 180 190 200
NYKPLQLVTA CSLDIYNFPF DVQNCSLTFT SWLHTIQDIN ITLWRSPEEV
210 220 230 240 250
RSDKSIFINQ GEWELLEVFP QFKEFSIDIS NSYAEMKFYV IIRRRPLFYA
260 270 280 290 300
VSLLLPSIFL MVVDIVGFCL PPDSGERVSF KITLLLGYSV FLIIVSDTLP
310 320 330 340 350
ATIGTPLIGV YFVVCMALLV ISLAETIFIV RLVHKQDLQR PVPDWLRHLV
360 370 380 390 400
LDRIAWILCL GEQPMAHRPP ATFQANKTDD CSGSDLLPAM GNHCSHVGGP
410 420 430 440 450
QDLEKTPRGR GSPLPPPREA SLAVRGLLQE LSSIRHFLEK RDEMREVARD
460 470 480
WLRVGYVLDR LLFRIYLLAV LAYSITLVTL WSIWHYS
Length:487
Mass (Da):56,056
Last modified:March 1, 1992 - v1
Checksum:iD0148867C8536D66
GO
Isoform 5-HT3R-AS (identifier: P23979-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     383-388: Missing.

Show »
Length:481
Mass (Da):55,474
Checksum:i7A9B7B5C38FEC909
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti31 – 311E → AR in CAA51089. (PubMed:7683998)Curated
Sequence conflicti31 – 311E → AR in CAA55870. (PubMed:7854052)Curated
Sequence conflicti31 – 311E → AR in CAA55871. (PubMed:7854052)Curated
Sequence conflicti74 – 741I → V in CAA51089. (PubMed:7683998)Curated
Sequence conflicti302 – 3021T → TA in CAA51089. (PubMed:7683998)Curated
Sequence conflicti302 – 3021T → TA in CAA55870. (PubMed:7854052)Curated
Sequence conflicti302 – 3021T → TA in CAA55871. (PubMed:7854052)Curated
Sequence conflicti384 – 3841S → SS in CAA80453. (PubMed:8112471)Curated
Sequence conflicti393 – 3931H → T in CAA55870. (PubMed:7854052)Curated
Sequence conflicti393 – 3931H → T in CAA55871. (PubMed:7854052)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei383 – 3886Missing in isoform 5-HT3R-AS. CuratedVSP_000079

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M74425 mRNA. Translation: AAA37124.1.
Z22772, Z22773 Genomic DNA. Translation: CAA80453.1.
X72395 mRNA. Translation: CAA51089.1.
X79283 Genomic DNA. Translation: CAA55870.1.
X79283 Genomic DNA. Translation: CAA55871.1.
PIRiS41757.
RefSeqiNP_038589.2. NM_013561.2.
UniGeneiMm.4831.

Genome annotation databases

GeneIDi15561.
KEGGimmu:15561.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M74425 mRNA. Translation: AAA37124.1 .
Z22772 , Z22773 Genomic DNA. Translation: CAA80453.1 .
X72395 mRNA. Translation: CAA51089.1 .
X79283 Genomic DNA. Translation: CAA55870.1 .
X79283 Genomic DNA. Translation: CAA55871.1 .
PIRi S41757.
RefSeqi NP_038589.2. NM_013561.2.
UniGenei Mm.4831.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4PIR X-ray 3.50 A/B/C/D/E 32-485 [» ]
ProteinModelPortali P23979.
SMRi P23979. Positions 45-335.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-48769N.
STRINGi 10090.ENSMUSP00000003826.

Chemistry

BindingDBi P23979.
ChEMBLi CHEMBL4972.
GuidetoPHARMACOLOGYi 373.

PTM databases

PhosphoSitei P23979.

Proteomic databases

MaxQBi P23979.
PRIDEi P23979.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 15561.
KEGGi mmu:15561.

Organism-specific databases

CTDi 3359.
MGIi MGI:96282. Htr3a.

Phylogenomic databases

eggNOGi NOG302526.
HOGENOMi HOG000241519.
HOVERGENi HBG106638.
InParanoidi P23979.
KOi K04819.
PhylomeDBi P23979.

Miscellaneous databases

NextBioi 288512.
PROi P23979.
SOURCEi Search...

Gene expression databases

CleanExi MM_HTR3A.
Genevestigatori P23979.

Family and domain databases

Gene3Di 1.20.120.370. 2 hits.
2.70.170.10. 1 hit.
InterProi IPR008132. 5HT3_rcpt.
IPR008133. 5HT3_rcpt_A.
IPR027361. Acetylcholine_rcpt_TM.
IPR006202. Neur_chan_lig-bd.
IPR006201. Neur_channel.
IPR006029. Neurotrans-gated_channel_TM.
IPR018000. Neurotransmitter_ion_chnl_CS.
[Graphical view ]
PANTHERi PTHR18945. PTHR18945. 1 hit.
Pfami PF02931. Neur_chan_LBD. 1 hit.
PF02932. Neur_chan_memb. 1 hit.
[Graphical view ]
PRINTSi PR01709. 5HT3ARECEPTR.
PR01708. 5HT3RECEPTOR.
PR00252. NRIONCHANNEL.
SUPFAMi SSF63712. SSF63712. 1 hit.
SSF90112. SSF90112. 1 hit.
TIGRFAMsi TIGR00860. LIC. 1 hit.
PROSITEi PS00236. NEUROTR_ION_CHANNEL. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Primary structure and functional expression of the 5HT3 receptor, a serotonin-gated ion channel."
    Maricq A.V., Peterson A.S., Brake A.J., Myers R.M., Julius D.
    Science 254:432-437(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Organisation of the murine 5-HT3 receptor gene and assignment to human chromosome 11."
    Uetz P., Abdelatty F., Villarroel A., Gundrun R., Weiss B., Koenen M.
    FEBS Lett. 339:302-306(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: BALB/c.
    Tissue: Brain.
  3. "Cloning and functional expression of an apparent splice variant of the murine 5-HT3 receptor A subunit."
    Hope A.G., Downie D.L., Sutherland L., Lambert J.J., Peters J.A., Burchell B.
    Eur. J. Pharmacol. 245:187-192(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: A/J.
  4. "Organization of the mouse 5-HT3 receptor gene and functional expression of two splice variants."
    Werner P., Kawashima E., Reid J., Hussy N., Lundstrom K., Buell G., Humbert Y., Jones K.A.
    Brain Res. Mol. Brain Res. 26:233-241(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], ALTERNATIVE SPLICING.
    Strain: 129/Sv.
  5. "A cytoplasmic region determines single-channel conductance in 5-HT3 receptors."
    Kelley S.P., Dunlop J.I., Kirkness E.F., Lambert J.J., Peters J.A.
    Nature 424:321-324(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, REGION.

Entry informationi

Entry namei5HT3A_MOUSE
AccessioniPrimary (citable) accession number: P23979
Secondary accession number(s): Q61225, Q61226
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: March 1, 1992
Last modified: October 29, 2014
This is version 129 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

The HA-stretch region of HTR3A seems to be responsible for the low conductance of HTR3A homomers compared to that of HTR3A/HTR3B heteromers.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3