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P23979 (5HT3A_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 127. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
5-hydroxytryptamine receptor 3A

Short name=5-HT3-A
Short name=5-HT3A
Alternative name(s):
5-hydroxytryptamine receptor 3
Short name=5-HT-3
Short name=5-HT3R
Serotonin receptor 3A
Serotonin-gated ion channel receptor
Gene names
Name:Htr3a
Synonyms:5ht3, Htr3
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length487 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. This receptor is a ligand-gated ion channel, which when activated causes fast, depolarizing responses in neurons. It is a cation-specific, but otherwise relatively nonselective, ion channel. Ref.5

Subunit structure

Forms pentahomomeric complex as well as pentaheteromeric complex with HTR3B; homomeric complex is functional but exhibits low conductance with modified voltage dependence, and decreased agonist and antagonist affinity. Interacts with RIC3 By similarity.

Subcellular location

Cell junctionsynapsepostsynaptic cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein.

Tissue specificity

Brain, spinal cord, and heart.

Miscellaneous

The HA-stretch region of HTR3A seems to be responsible for the low conductance of HTR3A homomers compared to that of HTR3A/HTR3B heteromers.

Sequence similarities

Belongs to the ligand-gated ion channel (TC 1.A.9) family. 5-hydroxytryptamine receptor (TC 1.A.9.2) subfamily. HTR3A sub-subfamily. [View classification]

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 5-HT3R-A (identifier: P23979-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 5-HT3R-AS (identifier: P23979-2)

The sequence of this isoform differs from the canonical sequence as follows:
     383-388: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2323 Potential
Chain24 – 4874645-hydroxytryptamine receptor 3A
PRO_0000000409

Regions

Topological domain24 – 245222Extracellular Potential
Transmembrane246 – 27227Helical; Name=1; Potential
Topological domain273 – 2775Cytoplasmic Potential
Transmembrane278 – 29619Helical; Name=2; Potential
Topological domain297 – 3059Extracellular Potential
Transmembrane306 – 32419Helical; Name=3; Potential
Topological domain325 – 464140Cytoplasmic Potential
Transmembrane465 – 48420Helical; Name=4; Potential
Topological domain485 – 4873Extracellular Potential
Region423 – 45937HA-stretch

Amino acid modifications

Glycosylation1081N-linked (GlcNAc...) Potential
Glycosylation1741N-linked (GlcNAc...) Potential
Glycosylation1901N-linked (GlcNAc...) Potential
Disulfide bond161 ↔ 175 By similarity

Natural variations

Alternative sequence383 – 3886Missing in isoform 5-HT3R-AS.
VSP_000079

Experimental info

Sequence conflict311E → AR in CAA51089. Ref.3
Sequence conflict311E → AR in CAA55870. Ref.4
Sequence conflict311E → AR in CAA55871. Ref.4
Sequence conflict741I → V in CAA51089. Ref.3
Sequence conflict3021T → TA in CAA51089. Ref.3
Sequence conflict3021T → TA in CAA55870. Ref.4
Sequence conflict3021T → TA in CAA55871. Ref.4
Sequence conflict3841S → SS in CAA80453. Ref.2
Sequence conflict3931H → T in CAA55870. Ref.4
Sequence conflict3931H → T in CAA55871. Ref.4

Sequences

Sequence LengthMass (Da)Tools
Isoform 5-HT3R-A [UniParc].

Last modified March 1, 1992. Version 1.
Checksum: D0148867C8536D66

FASTA48756,056
        10         20         30         40         50         60 
MRLCIPQVLL ALFLSMLTAP GEGSRRRATQ EDTTQPALLR LSDHLLANYK KGVRPVRDWR 

        70         80         90        100        110        120 
KPTTVSIDVI MYAILNVDEK NQVLTTYIWY RQYWTDEFLQ WTPEDFDNVT KLSIPTDSIW 

       130        140        150        160        170        180 
VPDILINEFV DVGKSPNIPY VYVHHRGEVQ NYKPLQLVTA CSLDIYNFPF DVQNCSLTFT 

       190        200        210        220        230        240 
SWLHTIQDIN ITLWRSPEEV RSDKSIFINQ GEWELLEVFP QFKEFSIDIS NSYAEMKFYV 

       250        260        270        280        290        300 
IIRRRPLFYA VSLLLPSIFL MVVDIVGFCL PPDSGERVSF KITLLLGYSV FLIIVSDTLP 

       310        320        330        340        350        360 
ATIGTPLIGV YFVVCMALLV ISLAETIFIV RLVHKQDLQR PVPDWLRHLV LDRIAWILCL 

       370        380        390        400        410        420 
GEQPMAHRPP ATFQANKTDD CSGSDLLPAM GNHCSHVGGP QDLEKTPRGR GSPLPPPREA 

       430        440        450        460        470        480 
SLAVRGLLQE LSSIRHFLEK RDEMREVARD WLRVGYVLDR LLFRIYLLAV LAYSITLVTL 


WSIWHYS 

« Hide

Isoform 5-HT3R-AS [UniParc].

Checksum: 7A9B7B5C38FEC909
Show »

FASTA48155,474

References

[1]"Primary structure and functional expression of the 5HT3 receptor, a serotonin-gated ion channel."
Maricq A.V., Peterson A.S., Brake A.J., Myers R.M., Julius D.
Science 254:432-437(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Organisation of the murine 5-HT3 receptor gene and assignment to human chromosome 11."
Uetz P., Abdelatty F., Villarroel A., Gundrun R., Weiss B., Koenen M.
FEBS Lett. 339:302-306(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: BALB/c.
Tissue: Brain.
[3]"Cloning and functional expression of an apparent splice variant of the murine 5-HT3 receptor A subunit."
Hope A.G., Downie D.L., Sutherland L., Lambert J.J., Peters J.A., Burchell B.
Eur. J. Pharmacol. 245:187-192(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: A/J.
[4]"Organization of the mouse 5-HT3 receptor gene and functional expression of two splice variants."
Werner P., Kawashima E., Reid J., Hussy N., Lundstrom K., Buell G., Humbert Y., Jones K.A.
Brain Res. Mol. Brain Res. 26:233-241(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], ALTERNATIVE SPLICING.
Strain: 129/Sv.
[5]"A cytoplasmic region determines single-channel conductance in 5-HT3 receptors."
Kelley S.P., Dunlop J.I., Kirkness E.F., Lambert J.J., Peters J.A.
Nature 424:321-324(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, REGION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M74425 mRNA. Translation: AAA37124.1.
Z22772, Z22773 Genomic DNA. Translation: CAA80453.1.
X72395 mRNA. Translation: CAA51089.1.
X79283 Genomic DNA. Translation: CAA55870.1.
X79283 Genomic DNA. Translation: CAA55871.1.
PIRS41757.
RefSeqNP_038589.2. NM_013561.2.
UniGeneMm.4831.

3D structure databases

ProteinModelPortalP23979.
SMRP23979. Positions 45-335.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-48769N.
STRING10090.ENSMUSP00000003826.

Chemistry

BindingDBP23979.
ChEMBLCHEMBL4972.
GuidetoPHARMACOLOGY373.

PTM databases

PhosphoSiteP23979.

Proteomic databases

PRIDEP23979.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID15561.
KEGGmmu:15561.

Organism-specific databases

CTD3359.
MGIMGI:96282. Htr3a.

Phylogenomic databases

eggNOGNOG302526.
HOGENOMHOG000241519.
HOVERGENHBG106638.
InParanoidP23979.
KOK04819.
PhylomeDBP23979.

Gene expression databases

CleanExMM_HTR3A.
GenevestigatorP23979.

Family and domain databases

Gene3D1.20.120.370. 2 hits.
2.70.170.10. 1 hit.
InterProIPR008132. 5HT3_rcpt.
IPR008133. 5HT3_rcpt_A.
IPR027361. Acetylcholine_rcpt_TM.
IPR006202. Neur_chan_lig-bd.
IPR006201. Neur_channel.
IPR006029. Neurotrans-gated_channel_TM.
IPR018000. Neurotransmitter_ion_chnl_CS.
[Graphical view]
PANTHERPTHR18945. PTHR18945. 1 hit.
PfamPF02931. Neur_chan_LBD. 1 hit.
PF02932. Neur_chan_memb. 1 hit.
[Graphical view]
PRINTSPR01709. 5HT3ARECEPTR.
PR01708. 5HT3RECEPTOR.
PR00252. NRIONCHANNEL.
SUPFAMSSF63712. SSF63712. 1 hit.
SSF90112. SSF90112. 1 hit.
TIGRFAMsTIGR00860. LIC. 1 hit.
PROSITEPS00236. NEUROTR_ION_CHANNEL. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio288512.
PROP23979.
SOURCESearch...

Entry information

Entry name5HT3A_MOUSE
AccessionPrimary (citable) accession number: P23979
Secondary accession number(s): Q61225, Q61226
Entry history
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: March 1, 1992
Last modified: June 11, 2014
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot