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P23978

- SC6A1_RAT

UniProt

P23978 - SC6A1_RAT

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Protein

Sodium- and chloride-dependent GABA transporter 1

Gene
Slc6a1, Gabt1, Gat-1, Gat1
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Terminates the action of GABA by its high affinity sodium-dependent reuptake into presynaptic terminals.

GO - Molecular functioni

  1. gamma-aminobutyric acid:sodium symporter activity Source: RGD
  2. neurotransmitter:sodium symporter activity Source: InterPro
  3. protein binding Source: RGD

GO - Biological processi

  1. gamma-aminobutyric acid import Source: RGD
  2. learning Source: RGD
  3. negative regulation of synaptic transmission, GABAergic Source: RGD
  4. positive regulation of gamma-aminobutyric acid secretion Source: RGD
  5. protein homooligomerization Source: RGD
  6. response to calcium ion Source: RGD
  7. response to cocaine Source: RGD
  8. response to estradiol Source: RGD
  9. response to lead ion Source: RGD
  10. response to organic cyclic compound Source: RGD
  11. response to organic substance Source: RGD
  12. response to organonitrogen compound Source: RGD
  13. response to purine-containing compound Source: RGD
  14. response to sucrose Source: RGD
  15. response to toxic substance Source: RGD
Complete GO annotation...

Keywords - Biological processi

Neurotransmitter transport, Symport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Sodium- and chloride-dependent GABA transporter 1
Short name:
GAT-1
Alternative name(s):
Solute carrier family 6 member 1
Gene namesi
Name:Slc6a1
Synonyms:Gabt1, Gat-1, Gat1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 4

Organism-specific databases

RGDi620533. Slc6a1.

Subcellular locationi

Cell membrane; Multi-pass membrane protein By similarity. Membrane; Multi-pass membrane protein
Note: Localized at the presynaptic terminals of interneurons By similarity. Localized at the plasma membrane and in a subset of intracellular vesicles.1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 5252Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei53 – 7321Helical; Name=1; Reviewed predictionAdd
BLAST
Transmembranei81 – 10020Helical; Name=2; Reviewed predictionAdd
BLAST
Transmembranei124 – 14421Helical; Name=3; Reviewed predictionAdd
BLAST
Topological domaini145 – 21167Extracellular Reviewed predictionAdd
BLAST
Transmembranei212 – 23019Helical; Name=4; Reviewed predictionAdd
BLAST
Transmembranei239 – 25618Helical; Name=5; Reviewed predictionAdd
BLAST
Transmembranei292 – 30918Helical; Name=6; Reviewed predictionAdd
BLAST
Transmembranei321 – 34222Helical; Name=7; Reviewed predictionAdd
BLAST
Transmembranei375 – 39420Helical; Name=8; Reviewed predictionAdd
BLAST
Transmembranei422 – 44019Helical; Name=9; Reviewed predictionAdd
BLAST
Transmembranei457 – 47721Helical; Name=10; Reviewed predictionAdd
BLAST
Transmembranei498 – 51720Helical; Name=11; Reviewed predictionAdd
BLAST
Transmembranei536 – 55419Helical; Name=12; Reviewed predictionAdd
BLAST
Topological domaini555 – 59945Cytoplasmic Reviewed predictionAdd
BLAST

GO - Cellular componenti

  1. axon Source: Ensembl
  2. cell surface Source: RGD
  3. integral component of plasma membrane Source: InterPro
  4. neuron projection Source: RGD
  5. plasma membrane Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 599599Sodium- and chloride-dependent GABA transporter 1PRO_0000214746Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi176 – 1761N-linked (GlcNAc...) Reviewed prediction
Glycosylationi181 – 1811N-linked (GlcNAc...) Reviewed prediction
Glycosylationi184 – 1841N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiP23978.
PRIDEiP23978.

PTM databases

PhosphoSiteiP23978.

Expressioni

Gene expression databases

GenevestigatoriP23978.

Interactioni

Subunit structurei

Interacts with MPP5.1 Publication

Protein-protein interaction databases

BioGridi249421. 1 interaction.
STRINGi10116.ENSRNOP00000009705.

Structurei

3D structure databases

ProteinModelPortaliP23978.
SMRiP23978. Positions 44-521.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi597 – 5993PDZ-binding

Domaini

The PDZ domain-binding motif is involved in the interaction with MPP5 By similarity.

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0733.
GeneTreeiENSGT00730000110773.
HOGENOMiHOG000116406.
HOVERGENiHBG071421.
InParanoidiP23978.
KOiK05034.
OMAiFFFNIVQ.
OrthoDBiEOG793B71.
PhylomeDBiP23978.
TreeFamiTF343812.

Family and domain databases

InterProiIPR000175. Na/ntran_symport.
IPR002980. Na/ntran_symport_GABA_GAT1.
[Graphical view]
PANTHERiPTHR11616. PTHR11616. 1 hit.
PfamiPF00209. SNF. 1 hit.
[Graphical view]
PRINTSiPR01195. GAT1TRNSPORT.
PR00176. NANEUSMPORT.
PROSITEiPS00610. NA_NEUROTRAN_SYMP_1. 1 hit.
PS00754. NA_NEUROTRAN_SYMP_2. 1 hit.
PS50267. NA_NEUROTRAN_SYMP_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P23978-1 [UniParc]FASTAAdd to Basket

« Hide

MATDNSKVAD GQISTEVSEA PVASDKPKTL VVKVQKKAGD LPDRDTWKGR    50
FDFLMSCVGY AIGLGNVWRF PYLCGKNGGG AFLIPYFLTL IFAGVPLFLL 100
ECSLGQYTSI GGLGVWKLAP MFKGVGLAAA VLSFWLNIYY IVIISWAIYY 150
LYNSFTTTLP WKQCDNPWNT DRCFSNYSLV NTTNMTSAVV EFWERNMHQM 200
TDGLDKPGQI RWPLAITLAI AWVLVYFCIW KGVGWTGKVV YFSATYPYIM 250
LIILFFRGVT LPGAKEGILF YITPNFRKLS DSEVWLDAAT QIFFSYGLGL 300
GSLIALGSYN SFHNNVYRDS IIVCCINSCT SMFAGFVIFS IVGFMAHVTK 350
RSIADVAASG PGLAFLAYPE AVTQLPISPL WAILFFSMLL MLGIDSQFCT 400
VEGFITALVD EYPRLLRNRR ELFIAAVCIV SYLIGLSNIT QGGIYVFKLF 450
DYYSASGMSL LFLVFFECVS ISWFYGVNRF YDNIQEMVGS RPCIWWKLCW 500
SFFTPIIVAG VFLFSAVQMT PLTMGSYVFP KWGQGVGWLM ALSSMVLIPG 550
YMAYMFLTLK GSLKQRLQVM IQPSEDIVRP ENGPEQPQAG SSASKEAYI 599
Length:599
Mass (Da):67,001
Last modified:March 1, 1992 - v1
Checksum:i4FEF85092DC1D045
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M59742 mRNA. Translation: AAA63487.1.
PIRiA35918. ACRTGT.
RefSeqiNP_077347.1. NM_024371.2.
XP_006237121.1. XM_006237059.1.
XP_006237122.1. XM_006237060.1.
UniGeneiRn.10035.
Rn.234542.

Genome annotation databases

EnsembliENSRNOT00000009705; ENSRNOP00000009705; ENSRNOG00000006527.
GeneIDi79212.
KEGGirno:79212.
UCSCiRGD:620533. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M59742 mRNA. Translation: AAA63487.1 .
PIRi A35918. ACRTGT.
RefSeqi NP_077347.1. NM_024371.2.
XP_006237121.1. XM_006237059.1.
XP_006237122.1. XM_006237060.1.
UniGenei Rn.10035.
Rn.234542.

3D structure databases

ProteinModelPortali P23978.
SMRi P23978. Positions 44-521.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 249421. 1 interaction.
STRINGi 10116.ENSRNOP00000009705.

Chemistry

BindingDBi P23978.
ChEMBLi CHEMBL4054.

PTM databases

PhosphoSitei P23978.

Proteomic databases

PaxDbi P23978.
PRIDEi P23978.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000009705 ; ENSRNOP00000009705 ; ENSRNOG00000006527 .
GeneIDi 79212.
KEGGi rno:79212.
UCSCi RGD:620533. rat.

Organism-specific databases

CTDi 6529.
RGDi 620533. Slc6a1.

Phylogenomic databases

eggNOGi COG0733.
GeneTreei ENSGT00730000110773.
HOGENOMi HOG000116406.
HOVERGENi HBG071421.
InParanoidi P23978.
KOi K05034.
OMAi FFFNIVQ.
OrthoDBi EOG793B71.
PhylomeDBi P23978.
TreeFami TF343812.

Miscellaneous databases

NextBioi 614608.
PROi P23978.

Gene expression databases

Genevestigatori P23978.

Family and domain databases

InterProi IPR000175. Na/ntran_symport.
IPR002980. Na/ntran_symport_GABA_GAT1.
[Graphical view ]
PANTHERi PTHR11616. PTHR11616. 1 hit.
Pfami PF00209. SNF. 1 hit.
[Graphical view ]
PRINTSi PR01195. GAT1TRNSPORT.
PR00176. NANEUSMPORT.
PROSITEi PS00610. NA_NEUROTRAN_SYMP_1. 1 hit.
PS00754. NA_NEUROTRAN_SYMP_2. 1 hit.
PS50267. NA_NEUROTRAN_SYMP_3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
    Tissue: Brain.
  2. "Analysis of the transmembrane topology and membrane assembly of the GAT-1 gamma-aminobutyric acid transporter."
    Clark J.A.
    J. Biol. Chem. 272:14695-14704(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY.
  3. "The GABA transporter GAT1 and the MAGUK protein Pals1: interaction, uptake modulation, and coexpression in the brain."
    McHugh E.M., Zhu W., Milgram S., Mager S.
    Mol. Cell. Neurosci. 26:406-417(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH MPP5, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiSC6A1_RAT
AccessioniPrimary (citable) accession number: P23978
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: March 1, 1992
Last modified: June 11, 2014
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

This protein is the target of psychomotor stimulants such as amphetamines or cocaine.

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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