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Protein

Sodium- and chloride-dependent GABA transporter 1

Gene

Slc6a1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Terminates the action of GABA by its high affinity sodium-dependent reuptake into presynaptic terminals.

GO - Molecular functioni

GO - Biological processi

  • gamma-aminobutyric acid import Source: RGD
  • learning Source: RGD
  • negative regulation of synaptic transmission, GABAergic Source: RGD
  • positive regulation of gamma-aminobutyric acid secretion Source: RGD
  • protein homooligomerization Source: RGD
  • response to calcium ion Source: RGD
  • response to cocaine Source: RGD
  • response to estradiol Source: RGD
  • response to lead ion Source: RGD
  • response to organic cyclic compound Source: RGD
  • response to organic substance Source: RGD
  • response to organonitrogen compound Source: RGD
  • response to purine-containing compound Source: RGD
  • response to sucrose Source: RGD
  • response to toxic substance Source: RGD
Complete GO annotation...

Keywords - Biological processi

Neurotransmitter transport, Symport, Transport

Enzyme and pathway databases

ReactomeiREACT_319222. Na+/Cl- dependent neurotransmitter transporters.
REACT_340081. Reuptake of GABA.

Names & Taxonomyi

Protein namesi
Recommended name:
Sodium- and chloride-dependent GABA transporter 1
Short name:
GAT-1
Alternative name(s):
Solute carrier family 6 member 1
Gene namesi
Name:Slc6a1
Synonyms:Gabt1, Gat-1, Gat1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Chromosome 4

Organism-specific databases

RGDi620533. Slc6a1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 5252CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei53 – 7321Helical; Name=1Sequence AnalysisAdd
BLAST
Transmembranei81 – 10020Helical; Name=2Sequence AnalysisAdd
BLAST
Transmembranei124 – 14421Helical; Name=3Sequence AnalysisAdd
BLAST
Topological domaini145 – 21167ExtracellularSequence AnalysisAdd
BLAST
Transmembranei212 – 23019Helical; Name=4Sequence AnalysisAdd
BLAST
Transmembranei239 – 25618Helical; Name=5Sequence AnalysisAdd
BLAST
Transmembranei292 – 30918Helical; Name=6Sequence AnalysisAdd
BLAST
Transmembranei321 – 34222Helical; Name=7Sequence AnalysisAdd
BLAST
Transmembranei375 – 39420Helical; Name=8Sequence AnalysisAdd
BLAST
Transmembranei422 – 44019Helical; Name=9Sequence AnalysisAdd
BLAST
Transmembranei457 – 47721Helical; Name=10Sequence AnalysisAdd
BLAST
Transmembranei498 – 51720Helical; Name=11Sequence AnalysisAdd
BLAST
Transmembranei536 – 55419Helical; Name=12Sequence AnalysisAdd
BLAST
Topological domaini555 – 59945CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  • axon Source: Ensembl
  • cell surface Source: RGD
  • integral component of plasma membrane Source: InterPro
  • neuron projection Source: RGD
  • plasma membrane Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 599599Sodium- and chloride-dependent GABA transporter 1PRO_0000214746Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi176 – 1761N-linked (GlcNAc...)Sequence Analysis
Glycosylationi181 – 1811N-linked (GlcNAc...)Sequence Analysis
Glycosylationi184 – 1841N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiP23978.
PRIDEiP23978.

PTM databases

PhosphoSiteiP23978.

Expressioni

Gene expression databases

GenevisibleiP23978. RN.

Interactioni

Subunit structurei

Interacts with MPP5.1 Publication

Protein-protein interaction databases

BioGridi249421. 1 interaction.
STRINGi10116.ENSRNOP00000009705.

Structurei

3D structure databases

ProteinModelPortaliP23978.
SMRiP23978. Positions 44-521.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi597 – 5993PDZ-binding

Domaini

The PDZ domain-binding motif is involved in the interaction with MPP5.By similarity

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0733.
GeneTreeiENSGT00760000118857.
HOGENOMiHOG000116406.
HOVERGENiHBG071421.
InParanoidiP23978.
KOiK05034.
OMAiTCITQGG.
OrthoDBiEOG793B71.
PhylomeDBiP23978.
TreeFamiTF343812.

Family and domain databases

InterProiIPR000175. Na/ntran_symport.
IPR002980. Na/ntran_symport_GABA_GAT1.
[Graphical view]
PANTHERiPTHR11616. PTHR11616. 1 hit.
PfamiPF00209. SNF. 1 hit.
[Graphical view]
PRINTSiPR01195. GAT1TRNSPORT.
PR00176. NANEUSMPORT.
PROSITEiPS00610. NA_NEUROTRAN_SYMP_1. 1 hit.
PS00754. NA_NEUROTRAN_SYMP_2. 1 hit.
PS50267. NA_NEUROTRAN_SYMP_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P23978-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATDNSKVAD GQISTEVSEA PVASDKPKTL VVKVQKKAGD LPDRDTWKGR
60 70 80 90 100
FDFLMSCVGY AIGLGNVWRF PYLCGKNGGG AFLIPYFLTL IFAGVPLFLL
110 120 130 140 150
ECSLGQYTSI GGLGVWKLAP MFKGVGLAAA VLSFWLNIYY IVIISWAIYY
160 170 180 190 200
LYNSFTTTLP WKQCDNPWNT DRCFSNYSLV NTTNMTSAVV EFWERNMHQM
210 220 230 240 250
TDGLDKPGQI RWPLAITLAI AWVLVYFCIW KGVGWTGKVV YFSATYPYIM
260 270 280 290 300
LIILFFRGVT LPGAKEGILF YITPNFRKLS DSEVWLDAAT QIFFSYGLGL
310 320 330 340 350
GSLIALGSYN SFHNNVYRDS IIVCCINSCT SMFAGFVIFS IVGFMAHVTK
360 370 380 390 400
RSIADVAASG PGLAFLAYPE AVTQLPISPL WAILFFSMLL MLGIDSQFCT
410 420 430 440 450
VEGFITALVD EYPRLLRNRR ELFIAAVCIV SYLIGLSNIT QGGIYVFKLF
460 470 480 490 500
DYYSASGMSL LFLVFFECVS ISWFYGVNRF YDNIQEMVGS RPCIWWKLCW
510 520 530 540 550
SFFTPIIVAG VFLFSAVQMT PLTMGSYVFP KWGQGVGWLM ALSSMVLIPG
560 570 580 590
YMAYMFLTLK GSLKQRLQVM IQPSEDIVRP ENGPEQPQAG SSASKEAYI
Length:599
Mass (Da):67,001
Last modified:March 1, 1992 - v1
Checksum:i4FEF85092DC1D045
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M59742 mRNA. Translation: AAA63487.1.
PIRiA35918. ACRTGT.
RefSeqiNP_077347.1. NM_024371.2.
XP_006237121.1. XM_006237059.1.
XP_006237122.1. XM_006237060.2.
UniGeneiRn.10035.
Rn.234542.

Genome annotation databases

EnsembliENSRNOT00000009705; ENSRNOP00000009705; ENSRNOG00000006527.
GeneIDi79212.
KEGGirno:79212.
UCSCiRGD:620533. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M59742 mRNA. Translation: AAA63487.1.
PIRiA35918. ACRTGT.
RefSeqiNP_077347.1. NM_024371.2.
XP_006237121.1. XM_006237059.1.
XP_006237122.1. XM_006237060.2.
UniGeneiRn.10035.
Rn.234542.

3D structure databases

ProteinModelPortaliP23978.
SMRiP23978. Positions 44-521.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi249421. 1 interaction.
STRINGi10116.ENSRNOP00000009705.

Chemistry

BindingDBiP23978.
ChEMBLiCHEMBL2111478.

PTM databases

PhosphoSiteiP23978.

Proteomic databases

PaxDbiP23978.
PRIDEiP23978.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000009705; ENSRNOP00000009705; ENSRNOG00000006527.
GeneIDi79212.
KEGGirno:79212.
UCSCiRGD:620533. rat.

Organism-specific databases

CTDi6529.
RGDi620533. Slc6a1.

Phylogenomic databases

eggNOGiCOG0733.
GeneTreeiENSGT00760000118857.
HOGENOMiHOG000116406.
HOVERGENiHBG071421.
InParanoidiP23978.
KOiK05034.
OMAiTCITQGG.
OrthoDBiEOG793B71.
PhylomeDBiP23978.
TreeFamiTF343812.

Enzyme and pathway databases

ReactomeiREACT_319222. Na+/Cl- dependent neurotransmitter transporters.
REACT_340081. Reuptake of GABA.

Miscellaneous databases

NextBioi614608.
PROiP23978.

Gene expression databases

GenevisibleiP23978. RN.

Family and domain databases

InterProiIPR000175. Na/ntran_symport.
IPR002980. Na/ntran_symport_GABA_GAT1.
[Graphical view]
PANTHERiPTHR11616. PTHR11616. 1 hit.
PfamiPF00209. SNF. 1 hit.
[Graphical view]
PRINTSiPR01195. GAT1TRNSPORT.
PR00176. NANEUSMPORT.
PROSITEiPS00610. NA_NEUROTRAN_SYMP_1. 1 hit.
PS00754. NA_NEUROTRAN_SYMP_2. 1 hit.
PS50267. NA_NEUROTRAN_SYMP_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
    Tissue: Brain.
  2. "Analysis of the transmembrane topology and membrane assembly of the GAT-1 gamma-aminobutyric acid transporter."
    Clark J.A.
    J. Biol. Chem. 272:14695-14704(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY.
  3. "The GABA transporter GAT1 and the MAGUK protein Pals1: interaction, uptake modulation, and coexpression in the brain."
    McHugh E.M., Zhu W., Milgram S., Mager S.
    Mol. Cell. Neurosci. 26:406-417(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH MPP5, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiSC6A1_RAT
AccessioniPrimary (citable) accession number: P23978
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: March 1, 1992
Last modified: June 24, 2015
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

This protein is the target of psychomotor stimulants such as amphetamines or cocaine.

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.