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P23978

- SC6A1_RAT

UniProt

P23978 - SC6A1_RAT

Protein

Sodium- and chloride-dependent GABA transporter 1

Gene

Slc6a1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 118 (01 Oct 2014)
      Sequence version 1 (01 Mar 1992)
      Previous versions | rss
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    Functioni

    Terminates the action of GABA by its high affinity sodium-dependent reuptake into presynaptic terminals.

    GO - Molecular functioni

    1. gamma-aminobutyric acid:sodium symporter activity Source: RGD
    2. neurotransmitter:sodium symporter activity Source: InterPro
    3. protein binding Source: RGD

    GO - Biological processi

    1. gamma-aminobutyric acid import Source: RGD
    2. learning Source: RGD
    3. negative regulation of synaptic transmission, GABAergic Source: RGD
    4. positive regulation of gamma-aminobutyric acid secretion Source: RGD
    5. protein homooligomerization Source: RGD
    6. response to calcium ion Source: RGD
    7. response to cocaine Source: RGD
    8. response to estradiol Source: RGD
    9. response to lead ion Source: RGD
    10. response to organic cyclic compound Source: RGD
    11. response to organic substance Source: RGD
    12. response to organonitrogen compound Source: RGD
    13. response to purine-containing compound Source: RGD
    14. response to sucrose Source: RGD
    15. response to toxic substance Source: RGD

    Keywords - Biological processi

    Neurotransmitter transport, Symport, Transport

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Sodium- and chloride-dependent GABA transporter 1
    Short name:
    GAT-1
    Alternative name(s):
    Solute carrier family 6 member 1
    Gene namesi
    Name:Slc6a1
    Synonyms:Gabt1, Gat-1, Gat1
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 4

    Organism-specific databases

    RGDi620533. Slc6a1.

    Subcellular locationi

    Cell membrane By similarity; Multi-pass membrane protein By similarity. Membrane 1 Publication; Multi-pass membrane protein 1 Publication
    Note: Localized at the presynaptic terminals of interneurons By similarity. Localized at the plasma membrane and in a subset of intracellular vesicles.By similarity

    GO - Cellular componenti

    1. axon Source: Ensembl
    2. cell surface Source: RGD
    3. integral component of plasma membrane Source: InterPro
    4. neuron projection Source: RGD
    5. plasma membrane Source: RGD

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 599599Sodium- and chloride-dependent GABA transporter 1PRO_0000214746Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi176 – 1761N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi181 – 1811N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi184 – 1841N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PaxDbiP23978.
    PRIDEiP23978.

    PTM databases

    PhosphoSiteiP23978.

    Expressioni

    Gene expression databases

    GenevestigatoriP23978.

    Interactioni

    Subunit structurei

    Interacts with MPP5.1 Publication

    Protein-protein interaction databases

    BioGridi249421. 1 interaction.
    STRINGi10116.ENSRNOP00000009705.

    Structurei

    3D structure databases

    ProteinModelPortaliP23978.
    SMRiP23978. Positions 44-521.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 5252CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini145 – 21167ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini555 – 59945CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei53 – 7321Helical; Name=1Sequence AnalysisAdd
    BLAST
    Transmembranei81 – 10020Helical; Name=2Sequence AnalysisAdd
    BLAST
    Transmembranei124 – 14421Helical; Name=3Sequence AnalysisAdd
    BLAST
    Transmembranei212 – 23019Helical; Name=4Sequence AnalysisAdd
    BLAST
    Transmembranei239 – 25618Helical; Name=5Sequence AnalysisAdd
    BLAST
    Transmembranei292 – 30918Helical; Name=6Sequence AnalysisAdd
    BLAST
    Transmembranei321 – 34222Helical; Name=7Sequence AnalysisAdd
    BLAST
    Transmembranei375 – 39420Helical; Name=8Sequence AnalysisAdd
    BLAST
    Transmembranei422 – 44019Helical; Name=9Sequence AnalysisAdd
    BLAST
    Transmembranei457 – 47721Helical; Name=10Sequence AnalysisAdd
    BLAST
    Transmembranei498 – 51720Helical; Name=11Sequence AnalysisAdd
    BLAST
    Transmembranei536 – 55419Helical; Name=12Sequence AnalysisAdd
    BLAST

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi597 – 5993PDZ-binding

    Domaini

    The PDZ domain-binding motif is involved in the interaction with MPP5.By similarity

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0733.
    GeneTreeiENSGT00730000110773.
    HOGENOMiHOG000116406.
    HOVERGENiHBG071421.
    InParanoidiP23978.
    KOiK05034.
    OMAiFFFNIVQ.
    OrthoDBiEOG793B71.
    PhylomeDBiP23978.
    TreeFamiTF343812.

    Family and domain databases

    InterProiIPR000175. Na/ntran_symport.
    IPR002980. Na/ntran_symport_GABA_GAT1.
    [Graphical view]
    PANTHERiPTHR11616. PTHR11616. 1 hit.
    PfamiPF00209. SNF. 1 hit.
    [Graphical view]
    PRINTSiPR01195. GAT1TRNSPORT.
    PR00176. NANEUSMPORT.
    PROSITEiPS00610. NA_NEUROTRAN_SYMP_1. 1 hit.
    PS00754. NA_NEUROTRAN_SYMP_2. 1 hit.
    PS50267. NA_NEUROTRAN_SYMP_3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P23978-1 [UniParc]FASTAAdd to Basket

    « Hide

    MATDNSKVAD GQISTEVSEA PVASDKPKTL VVKVQKKAGD LPDRDTWKGR    50
    FDFLMSCVGY AIGLGNVWRF PYLCGKNGGG AFLIPYFLTL IFAGVPLFLL 100
    ECSLGQYTSI GGLGVWKLAP MFKGVGLAAA VLSFWLNIYY IVIISWAIYY 150
    LYNSFTTTLP WKQCDNPWNT DRCFSNYSLV NTTNMTSAVV EFWERNMHQM 200
    TDGLDKPGQI RWPLAITLAI AWVLVYFCIW KGVGWTGKVV YFSATYPYIM 250
    LIILFFRGVT LPGAKEGILF YITPNFRKLS DSEVWLDAAT QIFFSYGLGL 300
    GSLIALGSYN SFHNNVYRDS IIVCCINSCT SMFAGFVIFS IVGFMAHVTK 350
    RSIADVAASG PGLAFLAYPE AVTQLPISPL WAILFFSMLL MLGIDSQFCT 400
    VEGFITALVD EYPRLLRNRR ELFIAAVCIV SYLIGLSNIT QGGIYVFKLF 450
    DYYSASGMSL LFLVFFECVS ISWFYGVNRF YDNIQEMVGS RPCIWWKLCW 500
    SFFTPIIVAG VFLFSAVQMT PLTMGSYVFP KWGQGVGWLM ALSSMVLIPG 550
    YMAYMFLTLK GSLKQRLQVM IQPSEDIVRP ENGPEQPQAG SSASKEAYI 599
    Length:599
    Mass (Da):67,001
    Last modified:March 1, 1992 - v1
    Checksum:i4FEF85092DC1D045
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M59742 mRNA. Translation: AAA63487.1.
    PIRiA35918. ACRTGT.
    RefSeqiNP_077347.1. NM_024371.2.
    XP_006237121.1. XM_006237059.1.
    XP_006237122.1. XM_006237060.1.
    UniGeneiRn.10035.
    Rn.234542.

    Genome annotation databases

    EnsembliENSRNOT00000009705; ENSRNOP00000009705; ENSRNOG00000006527.
    GeneIDi79212.
    KEGGirno:79212.
    UCSCiRGD:620533. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M59742 mRNA. Translation: AAA63487.1 .
    PIRi A35918. ACRTGT.
    RefSeqi NP_077347.1. NM_024371.2.
    XP_006237121.1. XM_006237059.1.
    XP_006237122.1. XM_006237060.1.
    UniGenei Rn.10035.
    Rn.234542.

    3D structure databases

    ProteinModelPortali P23978.
    SMRi P23978. Positions 44-521.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 249421. 1 interaction.
    STRINGi 10116.ENSRNOP00000009705.

    Chemistry

    BindingDBi P23978.
    ChEMBLi CHEMBL4054.

    PTM databases

    PhosphoSitei P23978.

    Proteomic databases

    PaxDbi P23978.
    PRIDEi P23978.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000009705 ; ENSRNOP00000009705 ; ENSRNOG00000006527 .
    GeneIDi 79212.
    KEGGi rno:79212.
    UCSCi RGD:620533. rat.

    Organism-specific databases

    CTDi 6529.
    RGDi 620533. Slc6a1.

    Phylogenomic databases

    eggNOGi COG0733.
    GeneTreei ENSGT00730000110773.
    HOGENOMi HOG000116406.
    HOVERGENi HBG071421.
    InParanoidi P23978.
    KOi K05034.
    OMAi FFFNIVQ.
    OrthoDBi EOG793B71.
    PhylomeDBi P23978.
    TreeFami TF343812.

    Miscellaneous databases

    NextBioi 614608.
    PROi P23978.

    Gene expression databases

    Genevestigatori P23978.

    Family and domain databases

    InterProi IPR000175. Na/ntran_symport.
    IPR002980. Na/ntran_symport_GABA_GAT1.
    [Graphical view ]
    PANTHERi PTHR11616. PTHR11616. 1 hit.
    Pfami PF00209. SNF. 1 hit.
    [Graphical view ]
    PRINTSi PR01195. GAT1TRNSPORT.
    PR00176. NANEUSMPORT.
    PROSITEi PS00610. NA_NEUROTRAN_SYMP_1. 1 hit.
    PS00754. NA_NEUROTRAN_SYMP_2. 1 hit.
    PS50267. NA_NEUROTRAN_SYMP_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
      Tissue: Brain.
    2. "Analysis of the transmembrane topology and membrane assembly of the GAT-1 gamma-aminobutyric acid transporter."
      Clark J.A.
      J. Biol. Chem. 272:14695-14704(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: TOPOLOGY.
    3. "The GABA transporter GAT1 and the MAGUK protein Pals1: interaction, uptake modulation, and coexpression in the brain."
      McHugh E.M., Zhu W., Milgram S., Mager S.
      Mol. Cell. Neurosci. 26:406-417(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH MPP5, SUBCELLULAR LOCATION.

    Entry informationi

    Entry nameiSC6A1_RAT
    AccessioniPrimary (citable) accession number: P23978
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 1, 1992
    Last sequence update: March 1, 1992
    Last modified: October 1, 2014
    This is version 118 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    This protein is the target of psychomotor stimulants such as amphetamines or cocaine.

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3