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P23978 (SC6A1_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 106. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Sodium- and chloride-dependent GABA transporter 1
Short name=GAT-1
Alternative name(s):
Solute carrier family 6 member 1
Gene names
Name:Slc6a1
Synonyms:Gabt1, Gat-1, Gat1
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length599 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Terminates the action of GABA by its high affinity sodium-dependent reuptake into presynaptic terminals.

Subunit structure

Interacts with MPP5. Ref.3

Subcellular location

Cell membrane; Multi-pass membrane protein By similarity. Membrane; Multi-pass membrane protein. Note: Localized at the presynaptic terminals of interneurons By similarity. Localized at the plasma membrane and in a subset of intracellular vesicles. Ref.3

Domain

The PDZ domain-binding motif is involved in the interaction with MPP5 By similarity.

Miscellaneous

This protein is the target of psychomotor stimulants such as amphetamines or cocaine.

Sequence similarities

Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A1 subfamily. [View classification]

Ontologies

Keywords
   Biological processNeurotransmitter transport
Symport
Transport
   Cellular componentCell membrane
Membrane
   DomainTransmembrane
Transmembrane helix
   PTMGlycoprotein
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processgamma-aminobutyric acid import

Inferred from direct assay PubMed 16378241. Source: RGD

learning

Inferred from mutant phenotype PubMed 12110474. Source: RGD

negative regulation of synaptic transmission, GABAergic

Inferred from mutant phenotype PubMed 17317750. Source: RGD

positive regulation of gamma-aminobutyric acid secretion

Inferred from mutant phenotype PubMed 14500747. Source: RGD

protein homooligomerization

Inferred from direct assay PubMed 16314925. Source: RGD

response to calcium ion

Inferred from expression pattern PubMed 15778221. Source: RGD

response to cocaine

Inferred from expression pattern PubMed 12832547. Source: RGD

response to estradiol stimulus

Inferred from expression pattern PubMed 15664431. Source: RGD

response to lead ion

Inferred from expression pattern PubMed 15149801. Source: RGD

response to purine-containing compound

Inferred from mutant phenotype PubMed 16730753. Source: RGD

response to sucrose stimulus

Inferred from expression pattern PubMed 15778221. Source: RGD

response to toxin

Inferred from mutant phenotype PubMed 14643747. Source: RGD

   Cellular_componentaxon

Inferred from electronic annotation. Source: Compara

cell surface

Inferred from direct assay PubMed 14500747. Source: RGD

integral to plasma membrane

Inferred from electronic annotation. Source: InterPro

neuron projection

Inferred from direct assay PubMed 15149801. Source: RGD

plasma membrane

Inferred from direct assay PubMed 16378241. Source: RGD

   Molecular_functiongamma-aminobutyric acid:sodium symporter activity

Inferred from direct assay Ref.1. Source: RGD

neurotransmitter:sodium symporter activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 599599Sodium- and chloride-dependent GABA transporter 1
PRO_0000214746

Regions

Topological domain1 – 5252Cytoplasmic Potential
Transmembrane53 – 7321Helical; Name=1; Potential
Transmembrane81 – 10020Helical; Name=2; Potential
Transmembrane124 – 14421Helical; Name=3; Potential
Topological domain145 – 21167Extracellular Potential
Transmembrane212 – 23019Helical; Name=4; Potential
Transmembrane239 – 25618Helical; Name=5; Potential
Transmembrane292 – 30918Helical; Name=6; Potential
Transmembrane321 – 34222Helical; Name=7; Potential
Transmembrane375 – 39420Helical; Name=8; Potential
Transmembrane422 – 44019Helical; Name=9; Potential
Transmembrane457 – 47721Helical; Name=10; Potential
Transmembrane498 – 51720Helical; Name=11; Potential
Transmembrane536 – 55419Helical; Name=12; Potential
Topological domain555 – 59945Cytoplasmic Potential
Motif597 – 5993PDZ-binding

Amino acid modifications

Glycosylation1761N-linked (GlcNAc...) Potential
Glycosylation1811N-linked (GlcNAc...) Potential
Glycosylation1841N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
P23978 [UniParc].

Last modified March 1, 1992. Version 1.
Checksum: 4FEF85092DC1D045

FASTA59967,001
        10         20         30         40         50         60 
MATDNSKVAD GQISTEVSEA PVASDKPKTL VVKVQKKAGD LPDRDTWKGR FDFLMSCVGY 

        70         80         90        100        110        120 
AIGLGNVWRF PYLCGKNGGG AFLIPYFLTL IFAGVPLFLL ECSLGQYTSI GGLGVWKLAP 

       130        140        150        160        170        180 
MFKGVGLAAA VLSFWLNIYY IVIISWAIYY LYNSFTTTLP WKQCDNPWNT DRCFSNYSLV 

       190        200        210        220        230        240 
NTTNMTSAVV EFWERNMHQM TDGLDKPGQI RWPLAITLAI AWVLVYFCIW KGVGWTGKVV 

       250        260        270        280        290        300 
YFSATYPYIM LIILFFRGVT LPGAKEGILF YITPNFRKLS DSEVWLDAAT QIFFSYGLGL 

       310        320        330        340        350        360 
GSLIALGSYN SFHNNVYRDS IIVCCINSCT SMFAGFVIFS IVGFMAHVTK RSIADVAASG 

       370        380        390        400        410        420 
PGLAFLAYPE AVTQLPISPL WAILFFSMLL MLGIDSQFCT VEGFITALVD EYPRLLRNRR 

       430        440        450        460        470        480 
ELFIAAVCIV SYLIGLSNIT QGGIYVFKLF DYYSASGMSL LFLVFFECVS ISWFYGVNRF 

       490        500        510        520        530        540 
YDNIQEMVGS RPCIWWKLCW SFFTPIIVAG VFLFSAVQMT PLTMGSYVFP KWGQGVGWLM 

       550        560        570        580        590 
ALSSMVLIPG YMAYMFLTLK GSLKQRLQVM IQPSEDIVRP ENGPEQPQAG SSASKEAYI

« Hide

References

[1]"Cloning and expression of a rat brain GABA transporter."
Guastella J., Nelson N., Nelson H., Czyzyk L., Keynan S., Miedel M.C., Davidson N., Lester H.A., Kanner B.I.
Science 249:1303-1306(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
Tissue: Brain.
[2]"Analysis of the transmembrane topology and membrane assembly of the GAT-1 gamma-aminobutyric acid transporter."
Clark J.A.
J. Biol. Chem. 272:14695-14704(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: TOPOLOGY.
[3]"The GABA transporter GAT1 and the MAGUK protein Pals1: interaction, uptake modulation, and coexpression in the brain."
McHugh E.M., Zhu W., Milgram S., Mager S.
Mol. Cell. Neurosci. 26:406-417(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH MPP5, SUBCELLULAR LOCATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		M59742 mRNA. Translation: AAA63487.1.
IPIIPI00187596.
PIRACRTGT. A35918.
RefSeqNP_077347.1. NM_024371.2.
UniGeneRn.10035.

3D structure databases

ProteinModelPortalP23978.
SMRP23978. Positions 44-532.
ModBaseSearch...

Protein-protein interaction databases

STRING10116.ENSRNOP00000009705.

PTM databases

PhosphoSiteP23978.

Proteomic databases

PaxDbP23978.
PRIDEP23978.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000009705; ENSRNOP00000009705; ENSRNOG00000006527.
GeneID79212.
KEGGrno:79212.
UCSCRGD:620533. rat.

Organism-specific databases

CTD6529.
RGD620533. Slc6a1.

Phylogenomic databases

eggNOGCOG0733.
GeneTreeENSGT00690000101944.
HOGENOMHOG000116406.
HOVERGENHBG071421.
InParanoidP23978.
KOK05034.
OMANSFTTEL.
OrthoDBEOG4JQ3X4.

Gene expression databases

GenevestigatorP23978.
GermOnlineENSRNOG00000006527. Rattus norvegicus.

Family and domain databases

InterProIPR000175. Na/ntran_symport.
IPR002980. Na/ntran_symport_GABA_GAT1.
[Graphical view]
PANTHERPTHR11616. PTHR11616. 1 hit.
PfamPF00209. SNF. 1 hit.
[Graphical view]
PRINTSPR01195. GAT1TRNSPORT.
PR00176. NANEUSMPORT.
PROSITEPS00610. NA_NEUROTRAN_SYMP_1. 1 hit.
PS00754. NA_NEUROTRAN_SYMP_2. 1 hit.
PS50267. NA_NEUROTRAN_SYMP_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

BindingDBP23978.
ChEMBLCHEMBL4054.
NextBio614608.

Entry information

Entry nameSC6A1_RAT
AccessionPrimary (citable) accession number: P23978
Entry history
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: March 1, 1992
Last modified: May 1, 2013
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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