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Protein

Enoyl-CoA delta isomerase 1, mitochondrial

Gene

Eci1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Able to isomerize both 3-cis and 3-trans double bonds into the 2-trans form in a range of enoyl-CoA species.

Catalytic activityi

A (3Z)-alk-3-enoyl-CoA = a (2E)-alk-2-enoyl-CoA.
A (3E)-alk-3-enoyl-CoA = a (2E)-alk-2-enoyl-CoA.

Pathwayi: fatty acid beta-oxidation

This protein is involved in the pathway fatty acid beta-oxidation, which is part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway fatty acid beta-oxidation and in Lipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei140Substrate; via amide nitrogenBy similarity1
Binding sitei164SubstrateBy similarity1
Sitei165Important for catalytic activityBy similarity1

GO - Molecular functioni

  • dodecenoyl-CoA delta-isomerase activity Source: RGD
  • enoyl-CoA hydratase activity Source: GO_Central
  • identical protein binding Source: RGD

GO - Biological processi

  • fatty acid beta-oxidation Source: RGD

Keywordsi

Molecular functionIsomerase
Biological processFatty acid metabolism, Lipid metabolism

Enzyme and pathway databases

BRENDAi5.3.3.8 5301
SABIO-RKP23965
UniPathwayiUPA00659

Chemistry databases

SwissLipidsiSLP:000001593

Names & Taxonomyi

Protein namesi
Recommended name:
Enoyl-CoA delta isomerase 1, mitochondrial (EC:5.3.3.8)
Alternative name(s):
3,2-trans-enoyl-CoA isomerase
Delta(3),Delta(2)-enoyl-CoA isomerase
Short name:
D3,D2-enoyl-CoA isomerase
Dodecenoyl-CoA isomerase
Gene namesi
Name:Eci1
Synonyms:Dci
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi61892 Eci1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi165E → Q: Loss of activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 28MitochondrionAdd BLAST28
ChainiPRO_000000742229 – 289Enoyl-CoA delta isomerase 1, mitochondrialAdd BLAST261

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei48N6-acetyllysine; alternateBy similarity1
Modified residuei48N6-succinyllysine; alternateBy similarity1
Modified residuei71N6-succinyllysineBy similarity1
Modified residuei76N6-acetyllysineBy similarity1
Modified residuei222N6-acetyllysine; alternateBy similarity1
Modified residuei222N6-succinyllysine; alternateBy similarity1
Modified residuei229N6-acetyllysine; alternateBy similarity1
Modified residuei229N6-succinyllysine; alternateBy similarity1
Modified residuei255N6-acetyllysine; alternateBy similarity1
Modified residuei255N6-succinyllysine; alternateBy similarity1
Modified residuei275N6-succinyllysineBy similarity1
Modified residuei283N6-acetyllysine; alternateBy similarity1
Modified residuei283N6-succinyllysine; alternateBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP23965
PRIDEiP23965

PTM databases

iPTMnetiP23965
PhosphoSitePlusiP23965

Interactioni

Subunit structurei

Homotrimer.1 Publication

GO - Molecular functioni

  • identical protein binding Source: RGD

Protein-protein interaction databases

IntActiP23965, 1 interactor
STRINGi10116.ENSRNOP00000011784

Structurei

Secondary structure

1289
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi32 – 37Combined sources6
Beta strandi44 – 49Combined sources6
Turni52 – 55Combined sources4
Helixi59 – 74Combined sources16
Beta strandi80 – 87Combined sources8
Beta strandi89 – 91Combined sources3
Helixi97 – 100Combined sources4
Helixi105 – 123Combined sources19
Beta strandi126 – 133Combined sources8
Beta strandi135 – 138Combined sources4
Helixi139 – 147Combined sources9
Beta strandi148 – 154Combined sources7
Helixi165 – 168Combined sources4
Helixi174 – 184Combined sources11
Helixi186 – 195Combined sources10
Helixi201 – 206Combined sources6
Beta strandi209 – 214Combined sources6
Helixi216 – 218Combined sources3
Helixi219 – 230Combined sources12
Helixi235 – 254Combined sources20
Helixi257 – 269Combined sources13
Helixi271 – 282Combined sources12

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1XX4X-ray2.20A29-289[»]
ProteinModelPortaliP23965
SMRiP23965
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP23965

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni93 – 97Substrate bindingBy similarity5

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiENOG410ISNQ Eukaryota
COG1024 LUCA
HOVERGENiHBG001112
InParanoidiP23965
PhylomeDBiP23965

Family and domain databases

InterProiView protein in InterPro
IPR029045 ClpP/crotonase-like_dom_sf
IPR018376 Enoyl-CoA_hyd/isom_CS
IPR001753 Enoyl-CoA_hydra/iso
PfamiView protein in Pfam
PF00378 ECH_1, 1 hit
SUPFAMiSSF52096 SSF52096, 1 hit
PROSITEiView protein in PROSITE
PS00166 ENOYL_COA_HYDRATASE, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P23965-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALAAARRVL LQAGSRLGRR GAVDGARRFS NKRVLVEKEG EAGIAVMKFK
60 70 80 90 100
NPPVNSLSLE FLTEFVISLE KLENDKSIRG VILTSERPGI FSAGLDLMEM
110 120 130 140 150
YGRNPAHYAE YWKAVQELWL RLYLSNLTLI SAINGASPAG GCLMALTCDY
160 170 180 190 200
RIMADNSKYT IGLNESLLGI VAPFWLKDNY VNTIGHRAAE RALQLGTLFP
210 220 230 240 250
PAEALKVGLV DEVVPEDQVH SKARSVMAKW FTIPDHSRQL TKSMMRKATA
260 270 280
DNLIKQREAD IQNFTSFISR DSIQKSLHVY LEKLKQKKG
Length:289
Mass (Da):32,254
Last modified:March 1, 1992 - v1
Checksum:i79D2CED09DD8FDC6
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti1 – 8MALAAARR → AGCCAC AA sequence (PubMed:2040594).Curated8
Sequence conflicti260D → N in AAA41073 (PubMed:2040594).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X61184 mRNA Translation: CAA43488.1
M61112 mRNA Translation: AAA41073.1
PIRiS17161
UniGeneiRn.80835

Genome annotation databases

UCSCiRGD:61892 rat

Similar proteinsi

Entry informationi

Entry nameiECI1_RAT
AccessioniPrimary (citable) accession number: P23965
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: March 1, 1992
Last modified: May 23, 2018
This is version 128 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

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