Reviewed, UniProtKB/Swiss-Prot P23955 (MPPA_NEUCR)

Names and origin

Protein names

Recommended name:
    Mitochondrial-processing peptidase subunit alpha
    EC=3.4.24.64
Alternative name(s):
    Alpha-MPP

Gene names

Name:	mpp
ORF Names:	NCU06270

Organism

Neurospora crassa [Complete proteome]

Taxonomic identifier

5141 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Sordariomycetes › Sordariomycetidae › Sordariales › Sordariaceae › Neurospora

Protein attributes

Sequence length	577 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Cleaves presequences (transit peptides) from mitochondrial protein precursors By similarity.
Catalytic activity	Release of N-terminal transit peptides from precursor proteins imported into the mitochondrion, typically with Arg in position P2.
Subunit structure	Heterodimer of alpha and beta subunits.
Subcellular location	Mitochondrion matrix.
Domain	Appears to contain two domains of approximately equal size which are separated by a loop-like sequence.
Sequence similarities	Belongs to the peptidase M16 family.
Caution	Does not seem to have a protease activity as it lack the zinc-binding site.

Ontologies

Keywords
Cellular component	Mitochondrion
Domain	Transit peptide
Molecular function	Hydrolase Metalloprotease Protease
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	metalloendopeptidase activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 35	35	Mitochondrion
	Chain	36 – 577	542	Mitochondrial-processing peptidase subunit alpha		PRO_0000026771
Regions
	Compositional bias	259 – 307	49	Ser-rich
Experimental info
	Sequence conflict	107	1	T → S AA sequence Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P23955-1 [UniParc]. Last modified March 1, 2004. Version 2. Checksum: 029491B2FBA01ADB	FASTA	577	63,041
	10 20 30 40 50 60 MLNRFRPARL VAQSSRCLPL TRARAGPLPV NNARTLATRA AAVNTKEPTE RDNITTLSNG 70 80 90 100 110 120 VRVASEDLPD AFSGVGVYID AGSRYENDYV RGASHIMDRL AFKSTSTRTA DEMLETVEKL 130 140 150 160 170 180 GGNIQCASSR ESMMYQAATF NKAIPTAVEL MAETIRDPKL TDEELEGQIM TAQYEVNEIW 190 200 210 220 230 240 SKAELILPEL VHMAAFKDNT LGNPLLCPKE RLDYINRDVI QTYRDAFYRP ERLVVAFAGV 250 260 270 280 290 300 PHERAVKLAE KYFGDMKASD APGLSRTGSE TSVDSLVSES SEASSESSSS SSDSSESSGG 310 320 330 340 350 360 LLSKLFSPKA KKATPNPFLT RVPISTEDLT RPAHYTGGFL TLPSQPPPLN PNLPTFTHIQ 370 380 390 400 410 420 LAFEGLAISD DDIYALATLQ TLLGGGGSFS AGGPGKGMYS RLYTNVLNQH GWVESCVAFN 430 440 450 460 470 480 HSYTDSGLFG IAASCYPGRT LPMLQVMCRE LHALTTDHGY SALGELEVSR AKNQLRSSLL 490 500 510 520 530 540 MNLESRMVEL EDLGRQVQVH GRKIPVREMT RRINELTVKD LRRVAKRVVG GMANNAGQGS 550 560 570 GAPTVVLQEA TVQGLKTTEL GWDQIQDTIA QWKLGRR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Matrix processing peptidase of mitochondria. Structure-function relationships." Schneider H., Arretz M., Wachter E., Neupert W. J. Biol. Chem. 265:9881-9887(1990) [PubMed: 2141023] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE OF N-TERMINUS.
[2]	"The genome sequence of the filamentous fungus Neurospora crassa." Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D., Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B., Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M., Qui D. , Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M., Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U., Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D., Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S., Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D., Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S., Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A., DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R., Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R., Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I., Sachs M.S., Lander E.S., Nusbaum C., Birren B.W. Nature 422:859-868(2003) [PubMed: 12712197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.

Cross-references

Sequence databases
EMBLGenBankDDBJ	J05484 mRNA. Translation: AAA33597.1. Sequence problems. AABX02000025 Genomic DNA. Translation: EAA33638.1.
PIR	A36442.
RefSeq	XP_962874.1.
3D structure databases
HSSP	HSSP built from PDB template 1HR6 based on UniProtKB P11914.
ModBase	Search...
Protein family/group databases
MEROPS	M16.971.
Genome annotation databases
GeneID	3879027.
KEGG	ncr:NCU06270.
NMPDR	fig|5141.1.peg.4058.
Enzyme and pathway databases
BioCyc	NCRA-XX3-01:NCRA-XX3-01-010018-MON.
BRENDA	3.4.24.64. 266.
Family and domain databases
InterPro	IPR011237. Pept_M16_core. IPR011765. Pept_M16_N. IPR001431. Pept_M16_Zn_BS. IPR007863. Peptidase_M16_C. [Graphical view]
Gene3D	G3DSA:3.30.830.10. Pept_M16_core. 2 hits.
Pfam	PF00675. Peptidase_M16. 1 hit. PF05193. Peptidase_M16_C. 1 hit. [Graphical view]
PROSITE	PS00143. INSULINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MPPA_NEUCR

Accession

Primary (citable) accession number: P23955
Secondary accession number(s): Q7RVH4

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 1992
Last sequence update:	March 1, 2004
Last modified:	January 20, 2009
This is version 81 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)