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P23954

- BLO2_KLEOX

UniProt

P23954 - BLO2_KLEOX

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Protein

Beta-lactamase OXY-2

Gene
bla
Organism
Klebsiella oxytoca
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Hydrolyzes broad-spectrum beta-lactam antibiotics. Active against all third-generation cephalosporins but ceftazidime.

Catalytic activityi

A beta-lactam + H2O = a substituted beta-amino acid.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei72 – 721Acyl-ester intermediate By similarity

GO - Molecular functioni

  1. beta-lactamase activity Source: UniProtKB-EC

GO - Biological processi

  1. beta-lactam antibiotic catabolic process Source: InterPro
  2. response to antibiotic Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Antibiotic resistance

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-lactamase OXY-2 (EC:3.5.2.6)
Alternative name(s):
Penicillinase
Gene namesi
Name:bla
OrganismiKlebsiella oxytoca
Taxonomic identifieri571 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeKlebsiella

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 27272 PublicationsAdd
BLAST
Chaini28 – 290263Beta-lactamase OXY-2PRO_0000016998Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP23954.
SMRiP23954. Positions 30-290.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni236 – 2383Substrate binding By similarity

Sequence similaritiesi

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.40.710.10. 1 hit.
InterProiIPR012338. Beta-lactam/transpept-like.
IPR000871. Beta-lactam_class-A/D.
IPR023650. Beta-lactam_class-A_AS.
[Graphical view]
PRINTSiPR00118. BLACTAMASEA.
SUPFAMiSSF56601. SSF56601. 1 hit.
PROSITEiPS00146. BETA_LACTAMASE_A. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P23954-1 [UniParc]FASTAAdd to Basket

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MIKSSWRKIA MLAAAVPLLL ASGALWASTD AIHQKLTDLE KRSGGRLGVA    50
LINTADNSQI LYRGDERFAM CSTSKVMAAA AVLKQSESNK EVVNKRLEIN 100
AADLVVWSPI TEKHLQSGMT LAELSAATLQ YSDNTAMNLI IGYLGGPEKV 150
TAFARSIGDA TFRLDRTEPT LNTAIPGDER DTSTPLAMAE SLRKLTLGDA 200
LGEQQRAQLV TWLKGNTTGG QSIRAGLPES WVVGDKTGAG DYGTTNDIAV 250
IWPEDHAPLV LVTYFTQPQQ DAKNRKEVLA AAAKIVTEGL 290
Length:290
Mass (Da):31,136
Last modified:October 1, 1996 - v2
Checksum:i134C4BC24E9999E7
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti15 – 151Missing in strain: KH11 and SB23.
Natural varianti199 – 1991D → N in strain: D488 and HB60.
Natural varianti225 – 2251A → V in strain: D488.
Natural varianti255 – 2551D → N in strain: D488.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z49084 Genomic DNA. Translation: CAA88908.1.
D84548 Genomic DNA. Translation: BAA12695.1.
Y17714 Genomic DNA. Translation: CAB42614.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z49084 Genomic DNA. Translation: CAA88908.1 .
D84548 Genomic DNA. Translation: BAA12695.1 .
Y17714 Genomic DNA. Translation: CAB42614.1 .

3D structure databases

ProteinModelPortali P23954.
SMRi P23954. Positions 30-290.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.40.710.10. 1 hit.
InterProi IPR012338. Beta-lactam/transpept-like.
IPR000871. Beta-lactam_class-A/D.
IPR023650. Beta-lactam_class-A_AS.
[Graphical view ]
PRINTSi PR00118. BLACTAMASEA.
SUPFAMi SSF56601. SSF56601. 1 hit.
PROSITEi PS00146. BETA_LACTAMASE_A. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Beta-lactamase gene promoters of 71 clinical strains of Klebsiella oxytoca."
    Fournier B., Lagrange P.H., Philippon A.
    Antimicrob. Agents Chemother. 40:460-463(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: SL911.
  2. "Molecular aspects of high-level resistance to sulbactam-cefoperazone in Klebsiella oxytoca clinical isolates."
    Kimura K., Arakawa Y., Ohsuka S., Ito H., Suzuki K., Kurokawa H., Kato N., Ohta M.
    Antimicrob. Agents Chemother. 40:1988-1994(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: SB23.
  3. "Genetic characterization of resistance to extended-spectrum beta-lactams in Klebsiella oxytoca isolates recovered from patients with septicemia at hospitals in the Stockholm area."
    Wu S.W., Dornbusch K., Kronvall G.
    Antimicrob. Agents Chemother. 43:1294-1297(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: KH11.
  4. "Cefotaxime-hydrolysing activity of the beta-lactamase of Klebsiella oxytoca D488 could be related to a threonine residue at position 140."
    Reynaud A., Peduzzi J., Barthelemy M., Labia R.
    FEMS Microbiol. Lett. 65:185-192(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 28-290.
    Strain: D488.
  5. "Characterization and amino acid sequence of the OXY-2 group beta-lactamase of pI 5.7 isolated from aztreonam-resistant Klebsiella oxytoca strain HB60."
    Farzaneh S., Peduzzi J., Sofer L., Reynaud A., Barthelemy M., Labia R.
    J. Antimicrob. Chemother. 40:789-795(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 28-290.
    Strain: HB60.

Entry informationi

Entry nameiBLO2_KLEOX
AccessioniPrimary (citable) accession number: P23954
Secondary accession number(s): P71418
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: October 1, 1996
Last modified: October 16, 2013
This is version 64 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

In strain KH11 OXY-2 is known as OXY-2a.

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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