P23954 (BLO2_KLEOX) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Beta-lactamase OXY-2
EC=3.5.2.6

Alternative name(s):
Penicillinase

Gene names

Name:

bla

Organism

Klebsiella oxytoca

Taxonomic identifier

571 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Klebsiella

Protein attributes

Sequence length	290 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Hydrolyzes broad-spectrum beta-lactam antibiotics. Active against all third-generation cephalosporins but ceftazidime.
Catalytic activity	A beta-lactam + H2O = a substituted beta-amino acid.
Miscellaneous	In strain KH11 OXY-2 is known as OXY-2a.
Sequence similarities	Belongs to the class-A beta-lactamase family.

Ontologies

Keywords
Biological process	Antibiotic resistance
Domain	Signal
Molecular function	Hydrolase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	beta-lactam antibiotic catabolic process Inferred from electronic annotation. Source: InterPro response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	beta-lactamase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 27	27	Ref.4 Ref.5
	Chain	28 – 290	263	Beta-lactamase OXY-2		PRO_0000016998
Regions
	Region	236 – 238	3	Substrate binding By similarity
Sites
	Active site	72	1	Acyl-ester intermediate By similarity
Natural variations
	Natural variant	15	1	Missing in strain: KH11 and SB23.
	Natural variant	199	1	D → N in strain: D488 and HB60.
	Natural variant	225	1	A → V in strain: D488.
	Natural variant	255	1	D → N in strain: D488.

Sequences

	Sequence		Length	Mass (Da)
	P23954 [UniParc]. Last modified October 1, 1996. Version 2. Checksum: 134C4BC24E9999E7	FASTA	290	31,136
	10 20 30 40 50 60 MIKSSWRKIA MLAAAVPLLL ASGALWASTD AIHQKLTDLE KRSGGRLGVA LINTADNSQI 70 80 90 100 110 120 LYRGDERFAM CSTSKVMAAA AVLKQSESNK EVVNKRLEIN AADLVVWSPI TEKHLQSGMT 130 140 150 160 170 180 LAELSAATLQ YSDNTAMNLI IGYLGGPEKV TAFARSIGDA TFRLDRTEPT LNTAIPGDER 190 200 210 220 230 240 DTSTPLAMAE SLRKLTLGDA LGEQQRAQLV TWLKGNTTGG QSIRAGLPES WVVGDKTGAG 250 260 270 280 290 DYGTTNDIAV IWPEDHAPLV LVTYFTQPQQ DAKNRKEVLA AAAKIVTEGL

References

[1]	"Beta-lactamase gene promoters of 71 clinical strains of Klebsiella oxytoca." Fournier B., Lagrange P.H., Philippon A. Antimicrob. Agents Chemother. 40:460-463(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: SL911.
[2]	"Molecular aspects of high-level resistance to sulbactam-cefoperazone in Klebsiella oxytoca clinical isolates." Kimura K., Arakawa Y., Ohsuka S., Ito H., Suzuki K., Kurokawa H., Kato N., Ohta M. Antimicrob. Agents Chemother. 40:1988-1994(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: SB23.
[3]	"Genetic characterization of resistance to extended-spectrum beta-lactams in Klebsiella oxytoca isolates recovered from patients with septicemia at hospitals in the Stockholm area." Wu S.W., Dornbusch K., Kronvall G. Antimicrob. Agents Chemother. 43:1294-1297(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: KH11.
[4]	"Cefotaxime-hydrolysing activity of the beta-lactamase of Klebsiella oxytoca D488 could be related to a threonine residue at position 140." Reynaud A., Peduzzi J., Barthelemy M., Labia R. FEMS Microbiol. Lett. 65:185-192(1991) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 28-290. Strain: D488.
[5]	"Characterization and amino acid sequence of the OXY-2 group beta-lactamase of pI 5.7 isolated from aztreonam-resistant Klebsiella oxytoca strain HB60." Farzaneh S., Peduzzi J., Sofer L., Reynaud A., Barthelemy M., Labia R. J. Antimicrob. Chemother. 40:789-795(1997) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 28-290. Strain: HB60.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z49084 Genomic DNA. Translation: CAA88908.1. D84548 Genomic DNA. Translation: BAA12695.1. Y17714 Genomic DNA. Translation: CAB42614.1.
3D structure databases
ProteinModelPortal	P23954.
SMR	P23954. Positions 30-290.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	3.40.710.10. 1 hit.
InterPro	IPR012338. Beta-lactam/transpept-like. IPR000871. Beta-lactam_class-A/D. IPR023650. Beta-lactam_class-A_AS. [Graphical view]
PRINTS	PR00118. BLACTAMASEA.
SUPFAM	SSF56601. PBP_transp_fold. 1 hit.
PROSITE	PS00146. BETA_LACTAMASE_A. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BLO2_KLEOX

Accession

Primary (citable) accession number: P23954
Secondary accession number(s): P71418

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 1992
Last sequence update:	October 1, 1996
Last modified:	April 3, 2013
This is version 63 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program