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P23954 (BLO2_KLEOX) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 64. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-lactamase OXY-2

EC=3.5.2.6
Alternative name(s):
Penicillinase
Gene names
Name:bla
OrganismKlebsiella oxytoca
Taxonomic identifier571 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeKlebsiella

Protein attributes

Sequence length290 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Hydrolyzes broad-spectrum beta-lactam antibiotics. Active against all third-generation cephalosporins but ceftazidime.

Catalytic activity

A beta-lactam + H2O = a substituted beta-amino acid.

Miscellaneous

In strain KH11 OXY-2 is known as OXY-2a.

Sequence similarities

Belongs to the class-A beta-lactamase family.

Ontologies

Keywords
   Biological processAntibiotic resistance
   DomainSignal
   Molecular functionHydrolase
   Technical termDirect protein sequencing
Gene Ontology (GO)
   Biological_processbeta-lactam antibiotic catabolic process

Inferred from electronic annotation. Source: InterPro

response to antibiotic

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionbeta-lactamase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2727 Ref.4 Ref.5
Chain28 – 290263Beta-lactamase OXY-2
PRO_0000016998

Regions

Region236 – 2383Substrate binding By similarity

Sites

Active site721Acyl-ester intermediate By similarity

Natural variations

Natural variant151Missing in strain: KH11 and SB23.
Natural variant1991D → N in strain: D488 and HB60.
Natural variant2251A → V in strain: D488.
Natural variant2551D → N in strain: D488.

Sequences

Sequence LengthMass (Da)Tools
P23954 [UniParc].

Last modified October 1, 1996. Version 2.
Checksum: 134C4BC24E9999E7

FASTA29031,136
        10         20         30         40         50         60 
MIKSSWRKIA MLAAAVPLLL ASGALWASTD AIHQKLTDLE KRSGGRLGVA LINTADNSQI 

        70         80         90        100        110        120 
LYRGDERFAM CSTSKVMAAA AVLKQSESNK EVVNKRLEIN AADLVVWSPI TEKHLQSGMT 

       130        140        150        160        170        180 
LAELSAATLQ YSDNTAMNLI IGYLGGPEKV TAFARSIGDA TFRLDRTEPT LNTAIPGDER 

       190        200        210        220        230        240 
DTSTPLAMAE SLRKLTLGDA LGEQQRAQLV TWLKGNTTGG QSIRAGLPES WVVGDKTGAG 

       250        260        270        280        290 
DYGTTNDIAV IWPEDHAPLV LVTYFTQPQQ DAKNRKEVLA AAAKIVTEGL 

« Hide

References

[1]"Beta-lactamase gene promoters of 71 clinical strains of Klebsiella oxytoca."
Fournier B., Lagrange P.H., Philippon A.
Antimicrob. Agents Chemother. 40:460-463(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: SL911.
[2]"Molecular aspects of high-level resistance to sulbactam-cefoperazone in Klebsiella oxytoca clinical isolates."
Kimura K., Arakawa Y., Ohsuka S., Ito H., Suzuki K., Kurokawa H., Kato N., Ohta M.
Antimicrob. Agents Chemother. 40:1988-1994(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: SB23.
[3]"Genetic characterization of resistance to extended-spectrum beta-lactams in Klebsiella oxytoca isolates recovered from patients with septicemia at hospitals in the Stockholm area."
Wu S.W., Dornbusch K., Kronvall G.
Antimicrob. Agents Chemother. 43:1294-1297(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: KH11.
[4]"Cefotaxime-hydrolysing activity of the beta-lactamase of Klebsiella oxytoca D488 could be related to a threonine residue at position 140."
Reynaud A., Peduzzi J., Barthelemy M., Labia R.
FEMS Microbiol. Lett. 65:185-192(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 28-290.
Strain: D488.
[5]"Characterization and amino acid sequence of the OXY-2 group beta-lactamase of pI 5.7 isolated from aztreonam-resistant Klebsiella oxytoca strain HB60."
Farzaneh S., Peduzzi J., Sofer L., Reynaud A., Barthelemy M., Labia R.
J. Antimicrob. Chemother. 40:789-795(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 28-290.
Strain: HB60.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z49084 Genomic DNA. Translation: CAA88908.1.
D84548 Genomic DNA. Translation: BAA12695.1.
Y17714 Genomic DNA. Translation: CAB42614.1.

3D structure databases

ProteinModelPortalP23954.
SMRP23954. Positions 30-290.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.40.710.10. 1 hit.
InterProIPR012338. Beta-lactam/transpept-like.
IPR000871. Beta-lactam_class-A/D.
IPR023650. Beta-lactam_class-A_AS.
[Graphical view]
PRINTSPR00118. BLACTAMASEA.
SUPFAMSSF56601. SSF56601. 1 hit.
PROSITEPS00146. BETA_LACTAMASE_A. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameBLO2_KLEOX
AccessionPrimary (citable) accession number: P23954
Secondary accession number(s): P71418
Entry history
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: October 1, 1996
Last modified: October 16, 2013
This is version 64 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families