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Protein

Beta-lactamase OXY-2

Gene

bla

Organism
Klebsiella oxytoca
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Hydrolyzes broad-spectrum beta-lactam antibiotics. Active against all third-generation cephalosporins but ceftazidime.

Catalytic activityi

A beta-lactam + H2O = a substituted beta-amino acid.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei72Acyl-ester intermediatePROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Antibiotic resistance

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-lactamase OXY-2 (EC:3.5.2.6)
Alternative name(s):
Penicillinase
Gene namesi
Name:bla
OrganismiKlebsiella oxytoca
Taxonomic identifieri571 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeKlebsiella

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 272 PublicationsAdd BLAST27
ChainiPRO_000001699828 – 290Beta-lactamase OXY-2Add BLAST263

Structurei

3D structure databases

ProteinModelPortaliP23954.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni236 – 238Substrate bindingBy similarity3

Sequence similaritiesi

Belongs to the class-A beta-lactamase family.Curated

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.40.710.10. 1 hit.
InterProiIPR012338. Beta-lactam/transpept-like.
IPR000871. Beta-lactam_class-A.
IPR023650. Beta-lactam_class-A_AS.
[Graphical view]
PRINTSiPR00118. BLACTAMASEA.
SUPFAMiSSF56601. SSF56601. 1 hit.
PROSITEiPS00146. BETA_LACTAMASE_A. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P23954-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIKSSWRKIA MLAAAVPLLL ASGALWASTD AIHQKLTDLE KRSGGRLGVA
60 70 80 90 100
LINTADNSQI LYRGDERFAM CSTSKVMAAA AVLKQSESNK EVVNKRLEIN
110 120 130 140 150
AADLVVWSPI TEKHLQSGMT LAELSAATLQ YSDNTAMNLI IGYLGGPEKV
160 170 180 190 200
TAFARSIGDA TFRLDRTEPT LNTAIPGDER DTSTPLAMAE SLRKLTLGDA
210 220 230 240 250
LGEQQRAQLV TWLKGNTTGG QSIRAGLPES WVVGDKTGAG DYGTTNDIAV
260 270 280 290
IWPEDHAPLV LVTYFTQPQQ DAKNRKEVLA AAAKIVTEGL
Length:290
Mass (Da):31,136
Last modified:October 1, 1996 - v2
Checksum:i134C4BC24E9999E7
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti15Missing in strain: KH11 and SB23. 1
Natural varianti199D → N in strain: D488 and HB60. 1
Natural varianti225A → V in strain: D488. 1
Natural varianti255D → N in strain: D488. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49084 Genomic DNA. Translation: CAA88908.1.
D84548 Genomic DNA. Translation: BAA12695.1.
Y17714 Genomic DNA. Translation: CAB42614.1.
RefSeqiWP_004099854.1. NZ_KQ088007.1.
WP_017146441.1. NZ_FLAE01000001.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49084 Genomic DNA. Translation: CAA88908.1.
D84548 Genomic DNA. Translation: BAA12695.1.
Y17714 Genomic DNA. Translation: CAB42614.1.
RefSeqiWP_004099854.1. NZ_KQ088007.1.
WP_017146441.1. NZ_FLAE01000001.1.

3D structure databases

ProteinModelPortaliP23954.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di3.40.710.10. 1 hit.
InterProiIPR012338. Beta-lactam/transpept-like.
IPR000871. Beta-lactam_class-A.
IPR023650. Beta-lactam_class-A_AS.
[Graphical view]
PRINTSiPR00118. BLACTAMASEA.
SUPFAMiSSF56601. SSF56601. 1 hit.
PROSITEiPS00146. BETA_LACTAMASE_A. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBLO2_KLEOX
AccessioniPrimary (citable) accession number: P23954
Secondary accession number(s): P71418
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: October 1, 1996
Last modified: November 2, 2016
This is version 70 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

In strain KH11 OXY-2 is known as OXY-2a.

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.