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P23954

- BLO2_KLEOX

UniProt

P23954 - BLO2_KLEOX

Protein

Beta-lactamase OXY-2

Gene

bla

Organism
Klebsiella oxytoca
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 65 (01 Oct 2014)
      Sequence version 2 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Hydrolyzes broad-spectrum beta-lactam antibiotics. Active against all third-generation cephalosporins but ceftazidime.

    Catalytic activityi

    A beta-lactam + H2O = a substituted beta-amino acid.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei72 – 721Acyl-ester intermediatePROSITE-ProRule annotation

    GO - Molecular functioni

    1. beta-lactamase activity Source: UniProtKB-EC

    GO - Biological processi

    1. beta-lactam antibiotic catabolic process Source: InterPro
    2. response to antibiotic Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Antibiotic resistance

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-lactamase OXY-2 (EC:3.5.2.6)
    Alternative name(s):
    Penicillinase
    Gene namesi
    Name:bla
    OrganismiKlebsiella oxytoca
    Taxonomic identifieri571 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeKlebsiella

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 27272 PublicationsAdd
    BLAST
    Chaini28 – 290263Beta-lactamase OXY-2PRO_0000016998Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliP23954.
    SMRiP23954. Positions 30-290.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni236 – 2383Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the class-A beta-lactamase family.Curated

    Keywords - Domaini

    Signal

    Family and domain databases

    Gene3Di3.40.710.10. 1 hit.
    InterProiIPR012338. Beta-lactam/transpept-like.
    IPR000871. Beta-lactam_class-A/D.
    IPR023650. Beta-lactam_class-A_AS.
    [Graphical view]
    PRINTSiPR00118. BLACTAMASEA.
    SUPFAMiSSF56601. SSF56601. 1 hit.
    PROSITEiPS00146. BETA_LACTAMASE_A. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P23954-1 [UniParc]FASTAAdd to Basket

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    MIKSSWRKIA MLAAAVPLLL ASGALWASTD AIHQKLTDLE KRSGGRLGVA    50
    LINTADNSQI LYRGDERFAM CSTSKVMAAA AVLKQSESNK EVVNKRLEIN 100
    AADLVVWSPI TEKHLQSGMT LAELSAATLQ YSDNTAMNLI IGYLGGPEKV 150
    TAFARSIGDA TFRLDRTEPT LNTAIPGDER DTSTPLAMAE SLRKLTLGDA 200
    LGEQQRAQLV TWLKGNTTGG QSIRAGLPES WVVGDKTGAG DYGTTNDIAV 250
    IWPEDHAPLV LVTYFTQPQQ DAKNRKEVLA AAAKIVTEGL 290
    Length:290
    Mass (Da):31,136
    Last modified:October 1, 1996 - v2
    Checksum:i134C4BC24E9999E7
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti15 – 151Missing in strain: KH11 and SB23.
    Natural varianti199 – 1991D → N in strain: D488 and HB60.
    Natural varianti225 – 2251A → V in strain: D488.
    Natural varianti255 – 2551D → N in strain: D488.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z49084 Genomic DNA. Translation: CAA88908.1.
    D84548 Genomic DNA. Translation: BAA12695.1.
    Y17714 Genomic DNA. Translation: CAB42614.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z49084 Genomic DNA. Translation: CAA88908.1 .
    D84548 Genomic DNA. Translation: BAA12695.1 .
    Y17714 Genomic DNA. Translation: CAB42614.1 .

    3D structure databases

    ProteinModelPortali P23954.
    SMRi P23954. Positions 30-290.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 3.40.710.10. 1 hit.
    InterProi IPR012338. Beta-lactam/transpept-like.
    IPR000871. Beta-lactam_class-A/D.
    IPR023650. Beta-lactam_class-A_AS.
    [Graphical view ]
    PRINTSi PR00118. BLACTAMASEA.
    SUPFAMi SSF56601. SSF56601. 1 hit.
    PROSITEi PS00146. BETA_LACTAMASE_A. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Beta-lactamase gene promoters of 71 clinical strains of Klebsiella oxytoca."
      Fournier B., Lagrange P.H., Philippon A.
      Antimicrob. Agents Chemother. 40:460-463(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: SL911.
    2. "Molecular aspects of high-level resistance to sulbactam-cefoperazone in Klebsiella oxytoca clinical isolates."
      Kimura K., Arakawa Y., Ohsuka S., Ito H., Suzuki K., Kurokawa H., Kato N., Ohta M.
      Antimicrob. Agents Chemother. 40:1988-1994(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: SB23.
    3. "Genetic characterization of resistance to extended-spectrum beta-lactams in Klebsiella oxytoca isolates recovered from patients with septicemia at hospitals in the Stockholm area."
      Wu S.W., Dornbusch K., Kronvall G.
      Antimicrob. Agents Chemother. 43:1294-1297(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: KH11.
    4. "Cefotaxime-hydrolysing activity of the beta-lactamase of Klebsiella oxytoca D488 could be related to a threonine residue at position 140."
      Reynaud A., Peduzzi J., Barthelemy M., Labia R.
      FEMS Microbiol. Lett. 65:185-192(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 28-290.
      Strain: D488.
    5. "Characterization and amino acid sequence of the OXY-2 group beta-lactamase of pI 5.7 isolated from aztreonam-resistant Klebsiella oxytoca strain HB60."
      Farzaneh S., Peduzzi J., Sofer L., Reynaud A., Barthelemy M., Labia R.
      J. Antimicrob. Chemother. 40:789-795(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 28-290.
      Strain: HB60.

    Entry informationi

    Entry nameiBLO2_KLEOX
    AccessioniPrimary (citable) accession number: P23954
    Secondary accession number(s): P71418
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 1, 1992
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 65 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    In strain KH11 OXY-2 is known as OXY-2a.

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3