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Protein

26 kDa endochitinase 2

Gene
N/A
Organism
Hordeum vulgare (Barley)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Defense against chitin containing fungal pathogens.

Catalytic activityi

Random hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.

GO - Molecular functioni

  1. chitinase activity Source: UniProtKB-EC

GO - Biological processi

  1. cell wall macromolecule catabolic process Source: InterPro
  2. chitin catabolic process Source: UniProtKB-KW
  3. defense response Source: UniProtKB-KW
  4. polysaccharide catabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Chitin degradation, Plant defense, Polysaccharide degradation

Enzyme and pathway databases

BRENDAi3.2.1.14. 2687.

Protein family/group databases

CAZyiGH19. Glycoside Hydrolase Family 19.

Names & Taxonomyi

Protein namesi
Recommended name:
26 kDa endochitinase 2 (EC:3.2.1.14)
Alternative name(s):
CHI-26
OrganismiHordeum vulgare (Barley)
Taxonomic identifieri4513 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladePooideaeTriticeaeHordeum

Organism-specific databases

GrameneiP23951.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2323Add
BLAST
Chaini24 – 26624326 kDa endochitinase 2PRO_0000005297Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi46 ↔ 108
Disulfide bondi120 ↔ 128
Disulfide bondi227 ↔ 259

Keywords - PTMi

Disulfide bond

Expressioni

Gene expression databases

ExpressionAtlasiP23951. baseline.
GenevestigatoriP23951.

Structurei

Secondary structure

1
266
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi25 – 273Combined sources
Helixi31 – 377Combined sources
Turni38 – 425Combined sources
Turni47 – 515Combined sources
Helixi54 – 618Combined sources
Turni65 – 684Combined sources
Beta strandi70 – 723Combined sources
Helixi73 – 9018Combined sources
Helixi102 – 1043Combined sources
Beta strandi124 – 1263Combined sources
Turni138 – 1414Combined sources
Helixi145 – 15511Combined sources
Turni159 – 1613Combined sources
Helixi165 – 1684Combined sources
Helixi170 – 18213Combined sources
Helixi191 – 1955Combined sources
Helixi203 – 2075Combined sources
Helixi214 – 22613Combined sources
Beta strandi227 – 2304Combined sources
Helixi233 – 24917Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1CNSX-ray1.91A/B24-266[»]
2BAAX-ray1.80A24-266[»]
SMRiP23951. Positions 24-266.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP23951.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Family and domain databases

InterProiIPR016283. Glyco_hydro_19.
IPR000726. Glyco_hydro_19_cat.
IPR023346. Lysozyme-like_dom.
[Graphical view]
PfamiPF00182. Glyco_hydro_19. 1 hit.
[Graphical view]
PIRSFiPIRSF001060. Endochitinase. 1 hit.
SUPFAMiSSF53955. SSF53955. 1 hit.
PROSITEiPS00773. CHITINASE_19_1. 1 hit.
PS00774. CHITINASE_19_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P23951-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRSLAVVVAV VATVAMAIGT ARGSVSSIVS RAQFDRMLLH RNDGACQAKG
60 70 80 90 100
FYTYDAFVAA AAAFPGFGTT GSADAQKREV AAFLAQTSHE TTGGWATAPD
110 120 130 140 150
GAFAWGYCFK QERGASSDYC TPSAQWPCAP GKRYYGRGPI QLSHNYNYGP
160 170 180 190 200
AGRAIGVDLL ANPDLVATDA TVGFKTAIWF WMTAQPPKPS SHAVIAGQWS
210 220 230 240 250
PSGADRAAGR VPGFGVITNI INGGIECGHG QDSRVADRIG FYKRYCDILG
260
VGYGNNLDCY SQRPFA
Length:266
Mass (Da):28,156
Last modified:February 29, 1992 - v1
Checksum:i0949BE88CC20B664
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti173 – 1731G → A AA sequence (PubMed:1899089).Curated
Sequence conflicti205 – 2051D → S AA sequence (PubMed:1899089).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti205 – 2051D → S.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L34210 Genomic DNA. Translation: AAA56786.1.
M62904 mRNA. Translation: AAA32941.1.
PIRiA29104.
A38664.
UniGeneiHv.23168.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L34210 Genomic DNA. Translation: AAA56786.1.
M62904 mRNA. Translation: AAA32941.1.
PIRiA29104.
A38664.
UniGeneiHv.23168.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1CNSX-ray1.91A/B24-266[»]
2BAAX-ray1.80A24-266[»]
SMRiP23951. Positions 24-266.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGH19. Glycoside Hydrolase Family 19.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

GrameneiP23951.

Enzyme and pathway databases

BRENDAi3.2.1.14. 2687.

Miscellaneous databases

EvolutionaryTraceiP23951.

Gene expression databases

ExpressionAtlasiP23951. baseline.
GenevestigatoriP23951.

Family and domain databases

InterProiIPR016283. Glyco_hydro_19.
IPR000726. Glyco_hydro_19_cat.
IPR023346. Lysozyme-like_dom.
[Graphical view]
PfamiPF00182. Glyco_hydro_19. 1 hit.
[Graphical view]
PIRSFiPIRSF001060. Endochitinase. 1 hit.
SUPFAMiSSF53955. SSF53955. 1 hit.
PROSITEiPS00773. CHITINASE_19_1. 1 hit.
PS00774. CHITINASE_19_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Biochemical and molecular characterization of three barley seed proteins with antifungal properties."
    Leah R., Tommerup H., Svendsen I., Mundy J.
    J. Biol. Chem. 266:1564-1573(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
    Strain: cv. Piggy.
  2. "Identification of a 28,000 dalton endochitinase in barley endosperm."
    Leah R., Mikkelsen J.D., Mundy J., Svendsen I.
    Carlsberg Res. Commun. 52:31-37(1986)
    Cited for: PRELIMINARY PROTEIN SEQUENCE OF 24-77 AND 148-188.
    Tissue: Endosperm.
  3. "Crystal structure of an endochitinase from Hordeum vulgare L. seeds."
    Hart P.J., Monzingo A.F., Ready M.P., Ernst S.R., Robertus J.D.
    J. Mol. Biol. 229:189-193(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS).
    Tissue: Seed.

Entry informationi

Entry nameiCHI2_HORVU
AccessioniPrimary (citable) accession number: P23951
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 29, 1992
Last sequence update: February 29, 1992
Last modified: March 31, 2015
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.