P23951 (CHI2_HORVU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: 26 kDa endochitinase 2 EC=3.2.1.14 Alternative name(s): CHI-26
Organism	Hordeum vulgare (Barley)
Taxonomic identifier	4513 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › Liliopsida › commelinids › Poales › Poaceae › BEP clade › Pooideae › Triticeae › Hordeum

Protein attributes

Sequence length	266 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Defense against chitin containing fungal pathogens.
Catalytic activity	Random hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.
Sequence similarities	Belongs to the glycosyl hydrolase 19 family. Chitinase class II subfamily.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Chitin degradation Plant defense Polysaccharide degradation
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Disulfide bond
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological_process	cell wall macromolecule catabolic process Inferred from electronic annotation. Source: InterPro chitin catabolic process Inferred from electronic annotation. Source: UniProtKB-KW defense response Inferred from electronic annotation. Source: UniProtKB-KW polysaccharide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	chitinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 23

23

Ref.2

Chain

24 – 266

243

26 kDa endochitinase 2

PRO_0000005297

Amino acid modifications

Disulfide bond

46 ↔ 108

Disulfide bond

120 ↔ 128

Disulfide bond

227 ↔ 259

Natural variations

Natural variant

205

1

D → S.

Experimental info

Sequence conflict

173

1

G → A AA sequence Ref.1

Sequence conflict

205

1

D → S AA sequence Ref.1

Secondary structure

1

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266

Helix Strand Turn

Details...

Helix

25 – 27

3

Helix

31 – 37

7

Turn

38 – 42

5

Turn

47 – 51

5

Helix

54 – 61

8

Turn

65 – 68

4

Beta strand

70 – 72

3

Helix

73 – 90

18

Helix

102 – 104

3

Beta strand

124 – 126

3

Turn

138 – 141

4

Helix

145 – 155

11

Turn

159 – 161

3

Helix

165 – 168

4

Helix

170 – 182

13

Helix

191 – 195

5

Helix

203 – 207

5

Helix

214 – 226

13

Beta strand

227 – 230

4

Helix

233 – 249

17

Sequences

	Sequence		Length	Mass (Da)
	P23951 [UniParc]. Last modified March 1, 1992. Version 1. Checksum: 0949BE88CC20B664	FASTA	266	28,156
	10 20 30 40 50 60 MRSLAVVVAV VATVAMAIGT ARGSVSSIVS RAQFDRMLLH RNDGACQAKG FYTYDAFVAA 70 80 90 100 110 120 AAAFPGFGTT GSADAQKREV AAFLAQTSHE TTGGWATAPD GAFAWGYCFK QERGASSDYC 130 140 150 160 170 180 TPSAQWPCAP GKRYYGRGPI QLSHNYNYGP AGRAIGVDLL ANPDLVATDA TVGFKTAIWF 190 200 210 220 230 240 WMTAQPPKPS SHAVIAGQWS PSGADRAAGR VPGFGVITNI INGGIECGHG QDSRVADRIG 250 260 FYKRYCDILG VGYGNNLDCY SQRPFA

References

[1]	"Biochemical and molecular characterization of three barley seed proteins with antifungal properties." Leah R., Tommerup H., Svendsen I., Mundy J. J. Biol. Chem. 266:1564-1573(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE. Strain: cv. Piggy.
[2]	"Identification of a 28,000 dalton endochitinase in barley endosperm." Leah R., Mikkelsen J.D., Mundy J., Svendsen I. Carlsberg Res. Commun. 52:31-37(1987) Cited for: PRELIMINARY PROTEIN SEQUENCE OF 24-77 AND 148-188. Tissue: Endosperm.
[3]	"Crystal structure of an endochitinase from Hordeum vulgare L. seeds." Hart P.J., Monzingo A.F., Ready M.P., Ernst S.R., Robertus J.D. J. Mol. Biol. 229:189-193(1993) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS). Tissue: Seed.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

L34210 Genomic DNA. Translation: AAA56786.1.
M62904 mRNA. Translation: AAA32941.1.

PIR

A29104.
A38664.

UniGene

Hv.23168.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1CNS	X-ray	1.91	A/B	24-266	[»]
2BAA	X-ray	1.80	A	24-266	[»]

ProteinModelPortal

P23951.

SMR

P23951. Positions 24-266.

ModBase

Search...

Protein family/group databases

CAZy

GH19. Glycoside Hydrolase Family 19.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Organism-specific databases

Gramene

P23951.

Gene expression databases

Genevestigator

P23951.

Family and domain databases

InterPro

IPR016283. Glyco_hydro_19.
IPR000726. Glyco_hydro_19_cat.
IPR023346. Lysozyme-like_dom.
[Graphical view]

Pfam

PF00182. Glyco_hydro_19. 1 hit.
[Graphical view]

PIRSF

PIRSF001060. Endochitinase. 1 hit.

SUPFAM

SSF53955. SSF53955. 1 hit.

PROSITE

PS00773. CHITINASE_19_1. 1 hit.
PS00774. CHITINASE_19_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P23951.

Entry information

Entry name

CHI2_HORVU

Accession

Primary (citable) accession number: P23951

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 1992
Last sequence update:	March 1, 1992
Last modified:	April 3, 2013
This is version 87 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program