Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P23951

- CHI2_HORVU

UniProt

P23951 - CHI2_HORVU

Protein

26 kDa endochitinase 2

Gene
N/A
Organism
Hordeum vulgare (Barley)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 89 (01 Oct 2014)
      Sequence version 1 (01 Mar 1992)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Defense against chitin containing fungal pathogens.

    Catalytic activityi

    Random hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.

    GO - Molecular functioni

    1. chitinase activity Source: UniProtKB-EC

    GO - Biological processi

    1. cell wall macromolecule catabolic process Source: InterPro
    2. chitin catabolic process Source: UniProtKB-KW
    3. defense response Source: UniProtKB-KW
    4. polysaccharide catabolic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Carbohydrate metabolism, Chitin degradation, Plant defense, Polysaccharide degradation

    Protein family/group databases

    CAZyiGH19. Glycoside Hydrolase Family 19.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    26 kDa endochitinase 2 (EC:3.2.1.14)
    Alternative name(s):
    CHI-26
    OrganismiHordeum vulgare (Barley)
    Taxonomic identifieri4513 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladePooideaeTriticeaeHordeum

    Organism-specific databases

    GrameneiP23951.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2323Add
    BLAST
    Chaini24 – 26624326 kDa endochitinase 2PRO_0000005297Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi46 ↔ 108
    Disulfide bondi120 ↔ 128
    Disulfide bondi227 ↔ 259

    Keywords - PTMi

    Disulfide bond

    Expressioni

    Gene expression databases

    GenevestigatoriP23951.

    Structurei

    Secondary structure

    1
    266
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi25 – 273
    Helixi31 – 377
    Turni38 – 425
    Turni47 – 515
    Helixi54 – 618
    Turni65 – 684
    Beta strandi70 – 723
    Helixi73 – 9018
    Helixi102 – 1043
    Beta strandi124 – 1263
    Turni138 – 1414
    Helixi145 – 15511
    Turni159 – 1613
    Helixi165 – 1684
    Helixi170 – 18213
    Helixi191 – 1955
    Helixi203 – 2075
    Helixi214 – 22613
    Beta strandi227 – 2304
    Helixi233 – 24917

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1CNSX-ray1.91A/B24-266[»]
    2BAAX-ray1.80A24-266[»]
    ProteinModelPortaliP23951.
    SMRiP23951. Positions 24-266.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP23951.

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Signal

    Family and domain databases

    InterProiIPR016283. Glyco_hydro_19.
    IPR000726. Glyco_hydro_19_cat.
    IPR023346. Lysozyme-like_dom.
    [Graphical view]
    PfamiPF00182. Glyco_hydro_19. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001060. Endochitinase. 1 hit.
    SUPFAMiSSF53955. SSF53955. 1 hit.
    PROSITEiPS00773. CHITINASE_19_1. 1 hit.
    PS00774. CHITINASE_19_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P23951-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRSLAVVVAV VATVAMAIGT ARGSVSSIVS RAQFDRMLLH RNDGACQAKG    50
    FYTYDAFVAA AAAFPGFGTT GSADAQKREV AAFLAQTSHE TTGGWATAPD 100
    GAFAWGYCFK QERGASSDYC TPSAQWPCAP GKRYYGRGPI QLSHNYNYGP 150
    AGRAIGVDLL ANPDLVATDA TVGFKTAIWF WMTAQPPKPS SHAVIAGQWS 200
    PSGADRAAGR VPGFGVITNI INGGIECGHG QDSRVADRIG FYKRYCDILG 250
    VGYGNNLDCY SQRPFA 266
    Length:266
    Mass (Da):28,156
    Last modified:March 1, 1992 - v1
    Checksum:i0949BE88CC20B664
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti173 – 1731G → A AA sequence (PubMed:1899089)Curated
    Sequence conflicti205 – 2051D → S AA sequence (PubMed:1899089)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti205 – 2051D → S.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L34210 Genomic DNA. Translation: AAA56786.1.
    M62904 mRNA. Translation: AAA32941.1.
    PIRiA29104.
    A38664.
    UniGeneiHv.23168.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L34210 Genomic DNA. Translation: AAA56786.1 .
    M62904 mRNA. Translation: AAA32941.1 .
    PIRi A29104.
    A38664.
    UniGenei Hv.23168.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1CNS X-ray 1.91 A/B 24-266 [» ]
    2BAA X-ray 1.80 A 24-266 [» ]
    ProteinModelPortali P23951.
    SMRi P23951. Positions 24-266.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GH19. Glycoside Hydrolase Family 19.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Organism-specific databases

    Gramenei P23951.

    Miscellaneous databases

    EvolutionaryTracei P23951.

    Gene expression databases

    Genevestigatori P23951.

    Family and domain databases

    InterProi IPR016283. Glyco_hydro_19.
    IPR000726. Glyco_hydro_19_cat.
    IPR023346. Lysozyme-like_dom.
    [Graphical view ]
    Pfami PF00182. Glyco_hydro_19. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001060. Endochitinase. 1 hit.
    SUPFAMi SSF53955. SSF53955. 1 hit.
    PROSITEi PS00773. CHITINASE_19_1. 1 hit.
    PS00774. CHITINASE_19_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Biochemical and molecular characterization of three barley seed proteins with antifungal properties."
      Leah R., Tommerup H., Svendsen I., Mundy J.
      J. Biol. Chem. 266:1564-1573(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
      Strain: cv. Piggy.
    2. "Identification of a 28,000 dalton endochitinase in barley endosperm."
      Leah R., Mikkelsen J.D., Mundy J., Svendsen I.
      Carlsberg Res. Commun. 52:31-37(1987)
      Cited for: PRELIMINARY PROTEIN SEQUENCE OF 24-77 AND 148-188.
      Tissue: Endosperm.
    3. "Crystal structure of an endochitinase from Hordeum vulgare L. seeds."
      Hart P.J., Monzingo A.F., Ready M.P., Ernst S.R., Robertus J.D.
      J. Mol. Biol. 229:189-193(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS).
      Tissue: Seed.

    Entry informationi

    Entry nameiCHI2_HORVU
    AccessioniPrimary (citable) accession number: P23951
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 1, 1992
    Last sequence update: March 1, 1992
    Last modified: October 1, 2014
    This is version 89 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Direct protein sequencing

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3