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P23951 (CHI2_HORVU) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 88. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
26 kDa endochitinase 2

EC=3.2.1.14
Alternative name(s):
CHI-26
OrganismHordeum vulgare (Barley)
Taxonomic identifier4513 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladePooideaeTriticeaeHordeum

Protein attributes

Sequence length266 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Defense against chitin containing fungal pathogens.

Catalytic activity

Random hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.

Sequence similarities

Belongs to the glycosyl hydrolase 19 family. Chitinase class II subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2323 Ref.2
Chain24 – 26624326 kDa endochitinase 2
PRO_0000005297

Amino acid modifications

Disulfide bond46 ↔ 108
Disulfide bond120 ↔ 128
Disulfide bond227 ↔ 259

Natural variations

Natural variant2051D → S.

Experimental info

Sequence conflict1731G → A AA sequence Ref.1
Sequence conflict2051D → S AA sequence Ref.1

Secondary structure

...................................... 266
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P23951 [UniParc].

Last modified March 1, 1992. Version 1.
Checksum: 0949BE88CC20B664

FASTA26628,156
        10         20         30         40         50         60 
MRSLAVVVAV VATVAMAIGT ARGSVSSIVS RAQFDRMLLH RNDGACQAKG FYTYDAFVAA 

        70         80         90        100        110        120 
AAAFPGFGTT GSADAQKREV AAFLAQTSHE TTGGWATAPD GAFAWGYCFK QERGASSDYC 

       130        140        150        160        170        180 
TPSAQWPCAP GKRYYGRGPI QLSHNYNYGP AGRAIGVDLL ANPDLVATDA TVGFKTAIWF 

       190        200        210        220        230        240 
WMTAQPPKPS SHAVIAGQWS PSGADRAAGR VPGFGVITNI INGGIECGHG QDSRVADRIG 

       250        260 
FYKRYCDILG VGYGNNLDCY SQRPFA 

« Hide

References

[1]"Biochemical and molecular characterization of three barley seed proteins with antifungal properties."
Leah R., Tommerup H., Svendsen I., Mundy J.
J. Biol. Chem. 266:1564-1573(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
Strain: cv. Piggy.
[2]"Identification of a 28,000 dalton endochitinase in barley endosperm."
Leah R., Mikkelsen J.D., Mundy J., Svendsen I.
Carlsberg Res. Commun. 52:31-37(1987)
Cited for: PRELIMINARY PROTEIN SEQUENCE OF 24-77 AND 148-188.
Tissue: Endosperm.
[3]"Crystal structure of an endochitinase from Hordeum vulgare L. seeds."
Hart P.J., Monzingo A.F., Ready M.P., Ernst S.R., Robertus J.D.
J. Mol. Biol. 229:189-193(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS).
Tissue: Seed.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L34210 Genomic DNA. Translation: AAA56786.1.
M62904 mRNA. Translation: AAA32941.1.
PIRA29104.
A38664.
UniGeneHv.23168.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1CNSX-ray1.91A/B24-266[»]
2BAAX-ray1.80A24-266[»]
ProteinModelPortalP23951.
SMRP23951. Positions 24-266.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyGH19. Glycoside Hydrolase Family 19.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Organism-specific databases

GrameneP23951.

Gene expression databases

GenevestigatorP23951.

Family and domain databases

InterProIPR016283. Glyco_hydro_19.
IPR000726. Glyco_hydro_19_cat.
IPR023346. Lysozyme-like_dom.
[Graphical view]
PfamPF00182. Glyco_hydro_19. 1 hit.
[Graphical view]
PIRSFPIRSF001060. Endochitinase. 1 hit.
SUPFAMSSF53955. SSF53955. 1 hit.
PROSITEPS00773. CHITINASE_19_1. 1 hit.
PS00774. CHITINASE_19_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP23951.

Entry information

Entry nameCHI2_HORVU
AccessionPrimary (citable) accession number: P23951
Entry history
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: March 1, 1992
Last modified: October 16, 2013
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries