SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P23951

- CHI2_HORVU

UniProt

P23951 - CHI2_HORVU

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
26 kDa endochitinase 2
Gene
N/A
Organism
Hordeum vulgare (Barley)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Defense against chitin containing fungal pathogens.

Catalytic activityi

Random hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.

GO - Molecular functioni

  1. chitinase activity Source: UniProtKB-EC

GO - Biological processi

  1. cell wall macromolecule catabolic process Source: InterPro
  2. chitin catabolic process Source: UniProtKB-KW
  3. defense response Source: UniProtKB-KW
  4. polysaccharide catabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Chitin degradation, Plant defense, Polysaccharide degradation

Protein family/group databases

CAZyiGH19. Glycoside Hydrolase Family 19.

Names & Taxonomyi

Protein namesi
Recommended name:
26 kDa endochitinase 2 (EC:3.2.1.14)
Alternative name(s):
CHI-26
OrganismiHordeum vulgare (Barley)
Taxonomic identifieri4513 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladePooideaeTriticeaeHordeum

Organism-specific databases

GrameneiP23951.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 23231 Publication
Add
BLAST
Chaini24 – 26624326 kDa endochitinase 2
PRO_0000005297Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi46 ↔ 108
Disulfide bondi120 ↔ 128
Disulfide bondi227 ↔ 259

Keywords - PTMi

Disulfide bond

Expressioni

Gene expression databases

GenevestigatoriP23951.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi25 – 273
Helixi31 – 377
Turni38 – 425
Turni47 – 515
Helixi54 – 618
Turni65 – 684
Beta strandi70 – 723
Helixi73 – 9018
Helixi102 – 1043
Beta strandi124 – 1263
Turni138 – 1414
Helixi145 – 15511
Turni159 – 1613
Helixi165 – 1684
Helixi170 – 18213
Helixi191 – 1955
Helixi203 – 2075
Helixi214 – 22613
Beta strandi227 – 2304
Helixi233 – 24917

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1CNSX-ray1.91A/B24-266[»]
2BAAX-ray1.80A24-266[»]
ProteinModelPortaliP23951.
SMRiP23951. Positions 24-266.

Miscellaneous databases

EvolutionaryTraceiP23951.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Family and domain databases

InterProiIPR016283. Glyco_hydro_19.
IPR000726. Glyco_hydro_19_cat.
IPR023346. Lysozyme-like_dom.
[Graphical view]
PfamiPF00182. Glyco_hydro_19. 1 hit.
[Graphical view]
PIRSFiPIRSF001060. Endochitinase. 1 hit.
SUPFAMiSSF53955. SSF53955. 1 hit.
PROSITEiPS00773. CHITINASE_19_1. 1 hit.
PS00774. CHITINASE_19_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P23951-1 [UniParc]FASTAAdd to Basket

« Hide

MRSLAVVVAV VATVAMAIGT ARGSVSSIVS RAQFDRMLLH RNDGACQAKG    50
FYTYDAFVAA AAAFPGFGTT GSADAQKREV AAFLAQTSHE TTGGWATAPD 100
GAFAWGYCFK QERGASSDYC TPSAQWPCAP GKRYYGRGPI QLSHNYNYGP 150
AGRAIGVDLL ANPDLVATDA TVGFKTAIWF WMTAQPPKPS SHAVIAGQWS 200
PSGADRAAGR VPGFGVITNI INGGIECGHG QDSRVADRIG FYKRYCDILG 250
VGYGNNLDCY SQRPFA 266
Length:266
Mass (Da):28,156
Last modified:March 1, 1992 - v1
Checksum:i0949BE88CC20B664
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti205 – 2051D → S.

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti173 – 1731G → A AA sequence 1 Publication
Sequence conflicti205 – 2051D → S AA sequence 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L34210 Genomic DNA. Translation: AAA56786.1.
M62904 mRNA. Translation: AAA32941.1.
PIRiA29104.
A38664.
UniGeneiHv.23168.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L34210 Genomic DNA. Translation: AAA56786.1 .
M62904 mRNA. Translation: AAA32941.1 .
PIRi A29104.
A38664.
UniGenei Hv.23168.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1CNS X-ray 1.91 A/B 24-266 [» ]
2BAA X-ray 1.80 A 24-266 [» ]
ProteinModelPortali P23951.
SMRi P23951. Positions 24-266.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi GH19. Glycoside Hydrolase Family 19.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Organism-specific databases

Gramenei P23951.

Miscellaneous databases

EvolutionaryTracei P23951.

Gene expression databases

Genevestigatori P23951.

Family and domain databases

InterProi IPR016283. Glyco_hydro_19.
IPR000726. Glyco_hydro_19_cat.
IPR023346. Lysozyme-like_dom.
[Graphical view ]
Pfami PF00182. Glyco_hydro_19. 1 hit.
[Graphical view ]
PIRSFi PIRSF001060. Endochitinase. 1 hit.
SUPFAMi SSF53955. SSF53955. 1 hit.
PROSITEi PS00773. CHITINASE_19_1. 1 hit.
PS00774. CHITINASE_19_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Biochemical and molecular characterization of three barley seed proteins with antifungal properties."
    Leah R., Tommerup H., Svendsen I., Mundy J.
    J. Biol. Chem. 266:1564-1573(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
    Strain: cv. Piggy.
  2. "Identification of a 28,000 dalton endochitinase in barley endosperm."
    Leah R., Mikkelsen J.D., Mundy J., Svendsen I.
    Carlsberg Res. Commun. 52:31-37(1987)
    Cited for: PRELIMINARY PROTEIN SEQUENCE OF 24-77 AND 148-188.
    Tissue: Endosperm.
  3. "Crystal structure of an endochitinase from Hordeum vulgare L. seeds."
    Hart P.J., Monzingo A.F., Ready M.P., Ernst S.R., Robertus J.D.
    J. Mol. Biol. 229:189-193(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS).
    Tissue: Seed.

Entry informationi

Entry nameiCHI2_HORVU
AccessioniPrimary (citable) accession number: P23951
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: March 1, 1992
Last modified: October 16, 2013
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi