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Protein

Zinc finger protein 36, C3H1 type-like 2

Gene

Zfp36l2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

mRNA-binding protein that plays a key role in self-renewal of erythroid cells in response to glucocorticoids. Specifically binds to the AU-rich element (ARE) in the 3'-UTR of target mRNAs, promoting their deadenylation and degradation. Specifically expressed in burst-forming unit-erythroid (BFU-E) progenitors in response to glucocorticoids and acts as a negative regulator of erythroid cell differentiation: promotes self-renewal of erythroid cells by binding mRNAs that are induced or highly expressed during terminal erythroid differentiation and promotes their degradation, preventing erythroid cell differentiation. Down-regulated during erythroid differentiation from the BFU-E stage, stabilizing mRNAs required for terminal differentiation.3 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri155 – 18329C3H1-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri193 – 22129C3H1-type 2PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

  • definitive hemopoiesis Source: UniProtKB
  • hemopoiesis Source: UniProtKB
  • mRNA catabolic process Source: UniProtKB
  • negative regulation of stem cell differentiation Source: UniProtKB
  • nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay Source: MGI
  • positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay Source: UniProtKB
  • regulation of mRNA stability Source: MGI
  • somatic stem cell division Source: UniProtKB
  • somatic stem cell population maintenance Source: UniProtKB
  • T cell differentiation in thymus Source: BHF-UCL
Complete GO annotation...

Keywords - Ligandi

DNA-binding, Metal-binding, RNA-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc finger protein 36, C3H1 type-like 2
Short name:
ZFP36-like 2
Alternative name(s):
Butyrate response factor 2
Protein TIS11D
Gene namesi
Name:Zfp36l2
Synonyms:Brf2, Tis11d
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:107945. Zfp36l2.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Lethality between 2 weeks of birth, due to pancytopenia. Peripheral blood shows a decrease in red and white cells, hemoglobin, hematocrit and platelets. Yolk sacs from embryonic day 11.5 (E11.5) and fetal livers from E14.5 display markedly reduced numbers of definitive multilineage and lineage-committed hematopoietic progenitors (PubMed:19633199). Mice lacking the N-terminus (DeltaN-Zfp36l2) display female infertility, with embryos that cannot progress beyond the 2-cell stage of development (PubMed:15342461). Mice lacking both Zfp36l1 and Zfp36l2 during thymopoiesis develop a T-cell acute lymphoblastic leukemia (PubMed:20622884).3 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 484484Zinc finger protein 36, C3H1 type-like 2PRO_0000089171Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei57 – 571PhosphoserineBy similarity
Modified residuei127 – 1271PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiP23949.
MaxQBiP23949.
PaxDbiP23949.
PeptideAtlasiP23949.
PRIDEiP23949.

PTM databases

iPTMnetiP23949.
PhosphoSiteiP23949.

Expressioni

Tissue specificityi

Expressed at highest level in lymphoid tissues such as thymus, spleen, lung, uterus, ovary, small and large intestine, mammary gland, fat and bone marrow. Expressed at intermediate level in kidney, heart, adrenal, eye and fetal liver. Weakly expressed in brain, skeletal muscle and liver.2 Publications

Developmental stagei

In embryos, expression is detected at E7, increases at E9.5, and then remains constant through E18.5. Expression in the yolk sac is relatively constant between E10.5 and E18.5. Placenta expression is also constant throughout development with a slight decrease observed at E18.5. Expressed in burst-forming unit-erythroid (BFU-E) progenitor cells and dow-regulated as erythroid cells differentiate.2 Publications

Inductioni

Expression is directly activated by NR3C1 in BFU-E progenitors. Down-regulated as erythroid cells differentiate.1 Publication

Gene expression databases

CleanExiMM_ZFP36L2.
GenevisibleiP23949. MM.

Interactioni

Protein-protein interaction databases

IntActiP23949. 3 interactions.
MINTiMINT-4137984.
STRINGi10090.ENSMUSP00000050820.

Structurei

3D structure databases

ProteinModelPortaliP23949.
SMRiP23949. Positions 153-222.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni172 – 21342RNA-bindingBy similarityAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi155 – 1606RNA-bindingBy similarity

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi93 – 964Poly-Gly
Compositional biasi140 – 1434Poly-Gln
Compositional biasi229 – 2324Poly-Gly
Compositional biasi292 – 2954Poly-Pro
Compositional biasi296 – 31924Ser-richAdd
BLAST
Compositional biasi326 – 3327Poly-Ala
Compositional biasi383 – 3897Poly-Ala
Compositional biasi401 – 44040Pro-richAdd
BLAST

Sequence similaritiesi

Contains 2 C3H1-type zinc fingers.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri155 – 18329C3H1-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri193 – 22129C3H1-type 2PROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1677. Eukaryota.
COG5063. LUCA.
GeneTreeiENSGT00530000063262.
HOGENOMiHOG000233479.
HOVERGENiHBG008483.
InParanoidiP23949.
KOiK18753.
OMAiQRDRPKL.
OrthoDBiEOG76QFJP.
TreeFamiTF315463.

Family and domain databases

Gene3Di4.10.1000.10. 2 hits.
InterProiIPR007635. Tis11B_N.
IPR000571. Znf_CCCH.
[Graphical view]
PfamiPF04553. Tis11B_N. 1 hit.
PF00642. zf-CCCH. 2 hits.
[Graphical view]
SMARTiSM00356. ZnF_C3H1. 2 hits.
[Graphical view]
SUPFAMiSSF90229. SSF90229. 2 hits.
PROSITEiPS50103. ZF_C3H1. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P23949-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTTLLSPFY DIDFLCKTEK SLANLNLNNM LDKKAVGTPV AAAPSSSFTP
60 70 80 90 100
GFLRRHSASN LHALAHPVPS PGSCSPKFPG APNGGGSSCG PAGGGGLASY
110 120 130 140 150
GQLKEPSGGS GTALVTKESK FRDRSFSENG ERSQHLLHLQ QQQKGGSGSQ
160 170 180 190 200
INSTRYKTEL CRPFEESGTC KYGEKCQFAH GFHELRSLTR HPKYKTELCR
210 220 230 240 250
TFHTIGFCPY GPRCHFIHNA DERRPAPSGG GGASGDLRAF GARDALHLGF
260 270 280 290 300
AREPRPKLHH SLSFSGFPSG HHQPPGGLES PLLLDSPTSR TPPPPSSSAS
310 320 330 340 350
SCSSSASSCS SASAASTPSG APTCCATAAA AALLYGPGGA EDLLSPGAPC
360 370 380 390 400
ASCSSSGANN AFAFGPELSS LITPLAIQTH NFAAAAAAAY YRNQQQGLTG
410 420 430 440 450
PAPPPAQPPA APAPPSPPFG FQLPRRLSES PVFDAPPSPP DSLSDRDSYL
460 470 480
SGSLSSGSLS GSESPSLDPG RRLPIFSRLS ISDD
Length:484
Mass (Da):50,070
Last modified:September 18, 2013 - v2
Checksum:iFBEB18B64775842A
GO

Sequence cautioni

The sequence AAA39709.1 differs from that shown. Reason: Erroneous gene model prediction. Curated
The sequence AAA72946.1 differs from that shown. Reason: Frameshift at position 385. Curated
The sequence AAA72946.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti413 – 4131A → T in BAE42014 (PubMed:16141072).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK160955 mRNA. Translation: BAE36112.1.
AK170763 mRNA. Translation: BAE42014.1.
AC132455 Genomic DNA. No translation available.
BC139416 mRNA. Translation: AAI39417.1.
M97165 Genomic DNA. Translation: AAA39709.1. Sequence problems.
M58564 mRNA. Translation: AAA72946.1. Sequence problems.
CCDSiCCDS28998.1.
PIRiC39590.
RefSeqiNP_001001806.1. NM_001001806.2.
UniGeneiMm.259321.

Genome annotation databases

EnsembliENSMUST00000060366; ENSMUSP00000050820; ENSMUSG00000045817.
GeneIDi12193.
KEGGimmu:12193.
UCSCiuc008dsq.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK160955 mRNA. Translation: BAE36112.1.
AK170763 mRNA. Translation: BAE42014.1.
AC132455 Genomic DNA. No translation available.
BC139416 mRNA. Translation: AAI39417.1.
M97165 Genomic DNA. Translation: AAA39709.1. Sequence problems.
M58564 mRNA. Translation: AAA72946.1. Sequence problems.
CCDSiCCDS28998.1.
PIRiC39590.
RefSeqiNP_001001806.1. NM_001001806.2.
UniGeneiMm.259321.

3D structure databases

ProteinModelPortaliP23949.
SMRiP23949. Positions 153-222.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP23949. 3 interactions.
MINTiMINT-4137984.
STRINGi10090.ENSMUSP00000050820.

PTM databases

iPTMnetiP23949.
PhosphoSiteiP23949.

Proteomic databases

EPDiP23949.
MaxQBiP23949.
PaxDbiP23949.
PeptideAtlasiP23949.
PRIDEiP23949.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000060366; ENSMUSP00000050820; ENSMUSG00000045817.
GeneIDi12193.
KEGGimmu:12193.
UCSCiuc008dsq.2. mouse.

Organism-specific databases

CTDi678.
MGIiMGI:107945. Zfp36l2.

Phylogenomic databases

eggNOGiKOG1677. Eukaryota.
COG5063. LUCA.
GeneTreeiENSGT00530000063262.
HOGENOMiHOG000233479.
HOVERGENiHBG008483.
InParanoidiP23949.
KOiK18753.
OMAiQRDRPKL.
OrthoDBiEOG76QFJP.
TreeFamiTF315463.

Miscellaneous databases

ChiTaRSiZfp36l2. mouse.
PROiP23949.
SOURCEiSearch...

Gene expression databases

CleanExiMM_ZFP36L2.
GenevisibleiP23949. MM.

Family and domain databases

Gene3Di4.10.1000.10. 2 hits.
InterProiIPR007635. Tis11B_N.
IPR000571. Znf_CCCH.
[Graphical view]
PfamiPF04553. Tis11B_N. 1 hit.
PF00642. zf-CCCH. 2 hits.
[Graphical view]
SMARTiSM00356. ZnF_C3H1. 2 hits.
[Graphical view]
SUPFAMiSSF90229. SSF90229. 2 hits.
PROSITEiPS50103. ZF_C3H1. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J and NOD.
    Tissue: Head.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  4. "Structure and expression of two mitogen inducible genes."
    Fletcher B.S.
    Thesis (1992), University of California Los Angeles, United States
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-50.
  5. "The TIS11 primary response gene is a member of a gene family that encodes proteins with a highly conserved sequence containing an unusual Cys-His repeat."
    Varnum B.C., Ma Q., Chi T., Fletcher B.S., Herschman H.R.
    Mol. Cell. Biol. 11:1754-1758(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 25-396.
    Strain: BALB/cJ.
  6. "Members of the tristetraprolin family of tandem CCCH zinc finger proteins exhibit CRM1-dependent nucleocytoplasmic shuttling."
    Phillips R.S., Ramos S.B., Blackshear P.J.
    J. Biol. Chem. 277:11606-11613(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  7. "The CCCH tandem zinc-finger protein Zfp36l2 is crucial for female fertility and early embryonic development."
    Ramos S.B., Stumpo D.J., Kennington E.A., Phillips R.S., Bock C.B., Ribeiro-Neto F., Blackshear P.J.
    Development 131:4883-4893(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE.
  8. "Targeted disruption of Zfp36l2, encoding a CCCH tandem zinc finger RNA-binding protein, results in defective hematopoiesis."
    Stumpo D.J., Broxmeyer H.E., Ward T., Cooper S., Hangoc G., Chung Y.J., Shelley W.C., Richfield E.K., Ray M.K., Yoder M.C., Aplan P.D., Blackshear P.J.
    Blood 114:2401-2410(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
  9. "Deletion of the RNA-binding proteins ZFP36L1 and ZFP36L2 leads to perturbed thymic development and T lymphoblastic leukemia."
    Hodson D.J., Janas M.L., Galloway A., Bell S.E., Andrews S., Li C.M., Pannell R., Siebel C.W., MacDonald H.R., De Keersmaecker K., Ferrando A.A., Grutz G., Turner M.
    Nat. Immunol. 11:717-724(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE.
  10. "Characterization of DeltaN-Zfp36l2 mutant associated with arrest of early embryonic development and female infertility."
    Ramos S.B.
    J. Biol. Chem. 287:13116-13127(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  11. "ZFP36L2 is required for self-renewal of early burst-forming unit erythroid progenitors."
    Zhang L., Prak L., Rayon-Estrada V., Thiru P., Flygare J., Lim B., Lodish H.F.
    Nature 499:92-96(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DEVELOPMENTAL STAGE, INDUCTION, RNA-BINDING.

Entry informationi

Entry nameiTISD_MOUSE
AccessioniPrimary (citable) accession number: P23949
Secondary accession number(s): B9EIF6, Q3TCE3, Q3TU59
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: September 18, 2013
Last modified: July 6, 2016
This is version 116 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.