Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Chymase

Gene

CMA1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Major secreted protease of mast cells with suspected roles in vasoactive peptide generation, extracellular matrix degradation, and regulation of gland secretion.

Catalytic activityi

Preferential cleavage: Phe-|-Xaa > Tyr-|-Xaa > Trp-|-Xaa > Leu-|-Xaa.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei66Charge relay system1
Active sitei110Charge relay system1
Active sitei203Charge relay system1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Enzyme and pathway databases

BioCyciZFISH:HS01750-MONOMER.
BRENDAi3.4.21.39. 2681.
ReactomeiR-HSA-1433557. Signaling by SCF-KIT.
R-HSA-1592389. Activation of Matrix Metalloproteinases.
R-HSA-2022377. Metabolism of Angiotensinogen to Angiotensins.
SABIO-RKP23946.

Protein family/group databases

MEROPSiS01.140.

Names & Taxonomyi

Protein namesi
Recommended name:
Chymase (EC:3.4.21.39)
Alternative name(s):
Alpha-chymase
Mast cell protease I
Gene namesi
Name:CMA1
Synonyms:CYH, CYM
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 14

Organism-specific databases

HGNCiHGNC:2097. CMA1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Organism-specific databases

DisGeNETi1215.
OpenTargetsiENSG00000092009.
PharmGKBiPA26623.

Chemistry databases

ChEMBLiCHEMBL4068.
GuidetoPHARMACOLOGYi2340.

Polymorphism and mutation databases

BioMutaiCMA1.
DMDMi126825.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 19Add BLAST19
PropeptideiPRO_000002743320 – 21Activation peptide1 Publication2
ChainiPRO_000002743422 – 247ChymaseAdd BLAST226

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi51 ↔ 67
Glycosylationi80N-linked (GlcNAc...)2 Publications1
Glycosylationi103N-linked (GlcNAc...)1 Publication1
Disulfide bondi144 ↔ 209
Disulfide bondi175 ↔ 188

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

PaxDbiP23946.
PeptideAtlasiP23946.
PRIDEiP23946.

PTM databases

iPTMnetiP23946.
PhosphoSitePlusiP23946.

Miscellaneous databases

PMAP-CutDBP23946.

Expressioni

Tissue specificityi

Mast cells in lung, heart, skin and placenta. Expressed in both normal skin and in urticaria pigmentosa lesions.1 Publication

Gene expression databases

BgeeiENSG00000092009.
CleanExiHS_CMA1.
GenevisibleiP23946. HS.

Organism-specific databases

HPAiCAB000363.
HPA052634.

Interactioni

Protein-protein interaction databases

BioGridi107624. 2 interactors.
STRINGi9606.ENSP00000250378.

Chemistry databases

BindingDBiP23946.

Structurei

Secondary structure

1247
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi36 – 42Combined sources7
Beta strandi44 – 47Combined sources4
Beta strandi49 – 57Combined sources9
Beta strandi60 – 63Combined sources4
Helixi65 – 67Combined sources3
Beta strandi70 – 77Combined sources8
Beta strandi79 – 83Combined sources5
Beta strandi89 – 98Combined sources10
Turni104 – 106Combined sources3
Beta strandi112 – 118Combined sources7
Beta strandi124 – 126Combined sources3
Beta strandi143 – 153Combined sources11
Beta strandi163 – 170Combined sources8
Helixi172 – 175Combined sources4
Turni183 – 185Combined sources3
Beta strandi186 – 190Combined sources5
Turni192 – 194Combined sources3
Beta strandi206 – 209Combined sources4
Beta strandi212 – 219Combined sources8
Beta strandi228 – 232Combined sources5
Helixi233 – 246Combined sources14

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1KLTX-ray1.90A22-247[»]
1NN6X-ray1.75A20-247[»]
1PJPX-ray2.20A22-247[»]
1T31X-ray1.90A22-247[»]
2HVXX-ray2.60A22-247[»]
3N7OX-ray1.80A22-247[»]
3S0NX-ray1.95A22-247[»]
4AFQX-ray1.51A/B22-247[»]
4AFSX-ray1.90A22-247[»]
4AFUX-ray1.82A/B22-247[»]
4AFZX-ray2.25A/B22-247[»]
4AG1X-ray1.40A22-247[»]
4AG2X-ray1.80A/B22-247[»]
4K2YX-ray2.30A22-247[»]
4K5ZX-ray1.80A22-247[»]
4K60X-ray1.50A22-247[»]
4K69X-ray1.50A22-247[»]
4KP0X-ray2.80A22-247[»]
ProteinModelPortaliP23946.
SMRiP23946.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP23946.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini22 – 245Peptidase S1PROSITE-ProRule annotationAdd BLAST224

Sequence similaritiesi

Belongs to the peptidase S1 family. Granzyme subfamily.PROSITE-ProRule annotation
Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG3627. Eukaryota.
COG5640. LUCA.
GeneTreeiENSGT00760000118895.
HOGENOMiHOG000251820.
HOVERGENiHBG013304.
InParanoidiP23946.
KOiK01329.
OMAiTLHHDIM.
OrthoDBiEOG091G0G5F.
PhylomeDBiP23946.
TreeFamiTF333630.

Family and domain databases

CDDicd00190. Tryp_SPc. 1 hit.
InterProiIPR009003. Peptidase_S1_PA.
IPR001314. Peptidase_S1A.
IPR001254. Trypsin_dom.
IPR018114. TRYPSIN_HIS.
IPR033116. TRYPSIN_SER.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P23946-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLLLPLPLLL FLLCSRAEAG EIIGGTECKP HSRPYMAYLE IVTSNGPSKF
60 70 80 90 100
CGGFLIRRNF VLTAAHCAGR SITVTLGAHN ITEEEDTWQK LEVIKQFRHP
110 120 130 140 150
KYNTSTLHHD IMLLKLKEKA SLTLAVGTLP FPSQFNFVPP GRMCRVAGWG
160 170 180 190 200
RTGVLKPGSD TLQEVKLRLM DPQACSHFRD FDHNLQLCVG NPRKTKSAFK
210 220 230 240
GDSGGPLLCA GVAQGIVSYG RSDAKPPAVF TRISHYRPWI NQILQAN
Length:247
Mass (Da):27,325
Last modified:March 1, 1992 - v1
Checksum:iDC1464A049ED6B00
GO
Isoform 2 (identifier: P23946-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-111: Missing.

Note: No experimental confirmation available.
Show »
Length:136
Mass (Da):14,893
Checksum:iDA8A15F89D222FC4
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti28C → S in AAB26828 (PubMed:8495723).Curated1
Sequence conflicti131 – 132FP → AV AA sequence (PubMed:8144971).Curated2

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_01177046G → R.Corresponds to variant rs5246dbSNPEnsembl.1
Natural variantiVAR_01177166H → R.1 PublicationCorresponds to variant rs5247dbSNPEnsembl.1
Natural variantiVAR_02919098R → H.Corresponds to variant rs13306252dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0569471 – 111Missing in isoform 2. 1 PublicationAdd BLAST111

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M64269 Genomic DNA. Translation: AAA52020.1.
M69137 Genomic DNA. Translation: AAA52021.1.
M69136 mRNA. Translation: AAA52019.1.
AB451464 mRNA. Translation: BAG70278.1.
AL132800 Genomic DNA. No translation available.
CH471078 Genomic DNA. Translation: EAW66007.1.
BC069110 mRNA. Translation: AAH69110.1.
BC069370 mRNA. Translation: AAH69370.1.
BC069490 mRNA. Translation: AAH69490.1.
BC103974 mRNA. Translation: AAI03975.1.
BC103975 mRNA. Translation: AAI03976.1.
S61334 mRNA. Translation: AAB26828.1.
X59072 Genomic DNA. Translation: CAA41796.1.
CCDSiCCDS76666.1. [P23946-2]
CCDS9630.1. [P23946-1]
PIRiA40967. KYHUCM.
RefSeqiNP_001295012.1. NM_001308083.1. [P23946-2]
NP_001827.1. NM_001836.4. [P23946-1]
UniGeneiHs.135626.

Genome annotation databases

EnsembliENST00000206446; ENSP00000206446; ENSG00000092009. [P23946-2]
ENST00000250378; ENSP00000250378; ENSG00000092009. [P23946-1]
GeneIDi1215.
KEGGihsa:1215.
UCSCiuc001wpp.2. human. [P23946-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M64269 Genomic DNA. Translation: AAA52020.1.
M69137 Genomic DNA. Translation: AAA52021.1.
M69136 mRNA. Translation: AAA52019.1.
AB451464 mRNA. Translation: BAG70278.1.
AL132800 Genomic DNA. No translation available.
CH471078 Genomic DNA. Translation: EAW66007.1.
BC069110 mRNA. Translation: AAH69110.1.
BC069370 mRNA. Translation: AAH69370.1.
BC069490 mRNA. Translation: AAH69490.1.
BC103974 mRNA. Translation: AAI03975.1.
BC103975 mRNA. Translation: AAI03976.1.
S61334 mRNA. Translation: AAB26828.1.
X59072 Genomic DNA. Translation: CAA41796.1.
CCDSiCCDS76666.1. [P23946-2]
CCDS9630.1. [P23946-1]
PIRiA40967. KYHUCM.
RefSeqiNP_001295012.1. NM_001308083.1. [P23946-2]
NP_001827.1. NM_001836.4. [P23946-1]
UniGeneiHs.135626.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1KLTX-ray1.90A22-247[»]
1NN6X-ray1.75A20-247[»]
1PJPX-ray2.20A22-247[»]
1T31X-ray1.90A22-247[»]
2HVXX-ray2.60A22-247[»]
3N7OX-ray1.80A22-247[»]
3S0NX-ray1.95A22-247[»]
4AFQX-ray1.51A/B22-247[»]
4AFSX-ray1.90A22-247[»]
4AFUX-ray1.82A/B22-247[»]
4AFZX-ray2.25A/B22-247[»]
4AG1X-ray1.40A22-247[»]
4AG2X-ray1.80A/B22-247[»]
4K2YX-ray2.30A22-247[»]
4K5ZX-ray1.80A22-247[»]
4K60X-ray1.50A22-247[»]
4K69X-ray1.50A22-247[»]
4KP0X-ray2.80A22-247[»]
ProteinModelPortaliP23946.
SMRiP23946.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi107624. 2 interactors.
STRINGi9606.ENSP00000250378.

Chemistry databases

BindingDBiP23946.
ChEMBLiCHEMBL4068.
GuidetoPHARMACOLOGYi2340.

Protein family/group databases

MEROPSiS01.140.

PTM databases

iPTMnetiP23946.
PhosphoSitePlusiP23946.

Polymorphism and mutation databases

BioMutaiCMA1.
DMDMi126825.

Proteomic databases

PaxDbiP23946.
PeptideAtlasiP23946.
PRIDEiP23946.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000206446; ENSP00000206446; ENSG00000092009. [P23946-2]
ENST00000250378; ENSP00000250378; ENSG00000092009. [P23946-1]
GeneIDi1215.
KEGGihsa:1215.
UCSCiuc001wpp.2. human. [P23946-1]

Organism-specific databases

CTDi1215.
DisGeNETi1215.
GeneCardsiCMA1.
HGNCiHGNC:2097. CMA1.
HPAiCAB000363.
HPA052634.
MIMi118938. gene.
neXtProtiNX_P23946.
OpenTargetsiENSG00000092009.
PharmGKBiPA26623.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3627. Eukaryota.
COG5640. LUCA.
GeneTreeiENSGT00760000118895.
HOGENOMiHOG000251820.
HOVERGENiHBG013304.
InParanoidiP23946.
KOiK01329.
OMAiTLHHDIM.
OrthoDBiEOG091G0G5F.
PhylomeDBiP23946.
TreeFamiTF333630.

Enzyme and pathway databases

BioCyciZFISH:HS01750-MONOMER.
BRENDAi3.4.21.39. 2681.
ReactomeiR-HSA-1433557. Signaling by SCF-KIT.
R-HSA-1592389. Activation of Matrix Metalloproteinases.
R-HSA-2022377. Metabolism of Angiotensinogen to Angiotensins.
SABIO-RKP23946.

Miscellaneous databases

EvolutionaryTraceiP23946.
GeneWikiiCMA1.
GenomeRNAii1215.
PMAP-CutDBP23946.
PROiP23946.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000092009.
CleanExiHS_CMA1.
GenevisibleiP23946. HS.

Family and domain databases

CDDicd00190. Tryp_SPc. 1 hit.
InterProiIPR009003. Peptidase_S1_PA.
IPR001314. Peptidase_S1A.
IPR001254. Trypsin_dom.
IPR018114. TRYPSIN_HIS.
IPR033116. TRYPSIN_SER.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCMA1_HUMAN
AccessioniPrimary (citable) accession number: P23946
Secondary accession number(s): B5BUM8
, Q16018, Q3SY36, Q3SY37, Q9UDH5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: March 1, 1992
Last modified: November 2, 2016
This is version 168 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 14
    Human chromosome 14: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. Peptidase families
    Classification of peptidase families and list of entries
  7. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.