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P23941

- MTB1_BACAM

UniProt

P23941 - MTB1_BACAM

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Protein

Modification methylase BamHI

Gene

bamHIM

Organism
Bacillus amyloliquefaciens (Bacillus velezensis)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

This methylase recognizes the double-stranded sequence GGATCC, causes specific methylation on C-5 on both strands, and protects the DNA from cleavage by the BamHI endonuclease.1 Publication

Catalytic activityi

S-adenosyl-L-methionine + DNA cytosine = S-adenosyl-L-homocysteine + DNA N(4)-methylcytosine.

GO - Molecular functioni

  1. DNA binding Source: InterPro
  2. N-methyltransferase activity Source: InterPro
  3. site-specific DNA-methyltransferase (cytosine-N4-specific) activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Restriction system

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BRENDAi2.1.1.113. 630.

Protein family/group databases

REBASEi3293. M.BamHI.

Names & Taxonomyi

Protein namesi
Recommended name:
Modification methylase BamHI (EC:2.1.1.113)
Short name:
M.BamHI
Alternative name(s):
N(4)- cytosine-specific methyltransferase BamHI
Gene namesi
Name:bamHIM
Encoded oniPlasmid0 Publication
OrganismiBacillus amyloliquefaciens (Bacillus velezensis)
Taxonomic identifieri1390 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 423423Modification methylase BamHIPRO_0000087920Add
BLAST

Family & Domainsi

Sequence similaritiesi

Belongs to the N(4)/N(6)-methyltransferase family.Curated

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR002295. D21N6_MeTrfase.
IPR002941. DNA_methylase_N4/N6.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PfamiPF01555. N6_N4_Mtase. 1 hit.
[Graphical view]
PRINTSiPR00506. D21N6MTFRASE.
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS00092. N6_MTASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P23941-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MRFFSVFDIV KNKANQLGYT ETEMYAVLKN YNVNKKDLLA YKENGVIPTD
60 70 80 90 100
KVLNGILSYL GMTKVELELK LGRIPAGLED VFLNNTKEIA KILENKNSVK
110 120 130 140 150
LNEFNSIQEI KPYFYTDLGK LYNGDCLELF KQVPDENVDT IFADPPFNLD
160 170 180 190 200
KEYDEGVTDK NSFSGYLDWY YKWIDECIRV LKPGGSLFIY NIPKWNTYLS
210 220 230 240 250
EYLNRKLNFR NWITVDMKFG LPIQNRLYPA NYSLLYYVKG DKPKTFNVQR
260 270 280 290 300
IPLQTCPHCG REIKDYGGYK NKMNPKGVTL SDVWSDIYPV RHSSSKNRKF
310 320 330 340 350
NELSVKLLDR IITMSTNEGD VVLDPFGGSG TTFAVSEMLG RKWIGFELGN
360 370 380 390 400
CEIIKERLKN KDKDKKLLGK VYEEKNKLFP NRVKELRKKN GLWIDDDFRQ
410 420
DHEGNSKGDK KNENNDQISL SLE
Length:423
Mass (Da):49,136
Last modified:September 21, 2011 - v3
Checksum:i8C2D9F58F33E6669
GO

Sequence cautioni

The sequence CAA38200.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X55285 Genomic DNA. Translation: CAA38998.2.
X54303 Genomic DNA. Translation: CAA38200.1. Different initiation.
PIRiS26843.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X55285 Genomic DNA. Translation: CAA38998.2 .
X54303 Genomic DNA. Translation: CAA38200.1 . Different initiation.
PIRi S26843.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein family/group databases

REBASEi 3293. M.BamHI.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

BRENDAi 2.1.1.113. 630.

Family and domain databases

Gene3Di 3.40.50.150. 1 hit.
InterProi IPR002295. D21N6_MeTrfase.
IPR002941. DNA_methylase_N4/N6.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR029063. SAM-dependent_MTases-like.
[Graphical view ]
Pfami PF01555. N6_N4_Mtase. 1 hit.
[Graphical view ]
PRINTSi PR00506. D21N6MTFRASE.
SUPFAMi SSF53335. SSF53335. 1 hit.
PROSITEi PS00092. N6_MTASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Characterization of the cloned BamHI restriction modification system: its nucleotide sequence, properties of the methylase, and expression in heterologous hosts."
    Brooks J.E., Nathan P.D., Landry D., Sznyter L.A., White-Rees P., Ives C.L., Moran L.S., Slatko B.E., Benner J.S.
    Nucleic Acids Res. 19:841-850(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-19, FUNCTION.
    Strain: H.
  2. Benner J.S.
    Submitted (DEC-2010) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION TO 28; 53; 167; 207 AND 348.
  3. "The complete sequence of the Bacillus amyloliquefaciens strain H, cellular BamHI methylase gene."
    Vanek P.G., Connaughton J.F., Kaloss W.D., Chirikjian J.G.
    Nucleic Acids Res. 18:6145-6145(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 24-423.
    Strain: H.

Entry informationi

Entry nameiMTB1_BACAM
AccessioniPrimary (citable) accession number: P23941
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: September 21, 2011
Last modified: October 1, 2014
This is version 83 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing, Plasmid

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3