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P23941

- MTB1_BACAM

UniProt

P23941 - MTB1_BACAM

Protein

Modification methylase BamHI

Gene

bamHIM

Organism
Bacillus amyloliquefaciens (Bacillus velezensis)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 83 (01 Oct 2014)
      Sequence version 3 (21 Sep 2011)
      Previous versions | rss
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    Functioni

    This methylase recognizes the double-stranded sequence GGATCC, causes specific methylation on C-5 on both strands, and protects the DNA from cleavage by the BamHI endonuclease.1 Publication

    Catalytic activityi

    S-adenosyl-L-methionine + DNA cytosine = S-adenosyl-L-homocysteine + DNA N(4)-methylcytosine.

    GO - Molecular functioni

    1. DNA binding Source: InterPro
    2. N-methyltransferase activity Source: InterPro
    3. site-specific DNA-methyltransferase (cytosine-N4-specific) activity Source: UniProtKB-EC

    GO - Biological processi

    1. DNA restriction-modification system Source: UniProtKB-KW

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    Restriction system

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Enzyme and pathway databases

    BRENDAi2.1.1.113. 630.

    Protein family/group databases

    REBASEi3293. M.BamHI.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Modification methylase BamHI (EC:2.1.1.113)
    Short name:
    M.BamHI
    Alternative name(s):
    N(4)- cytosine-specific methyltransferase BamHI
    Gene namesi
    Name:bamHIM
    Encoded oniPlasmid0 Publication
    OrganismiBacillus amyloliquefaciens (Bacillus velezensis)
    Taxonomic identifieri1390 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 423423Modification methylase BamHIPRO_0000087920Add
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the N(4)/N(6)-methyltransferase family.Curated

    Family and domain databases

    Gene3Di3.40.50.150. 1 hit.
    InterProiIPR002295. D21N6_MeTrfase.
    IPR002941. DNA_methylase_N4/N6.
    IPR002052. DNA_methylase_N6_adenine_CS.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view]
    PfamiPF01555. N6_N4_Mtase. 1 hit.
    [Graphical view]
    PRINTSiPR00506. D21N6MTFRASE.
    SUPFAMiSSF53335. SSF53335. 1 hit.
    PROSITEiPS00092. N6_MTASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P23941-1 [UniParc]FASTAAdd to Basket

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    MRFFSVFDIV KNKANQLGYT ETEMYAVLKN YNVNKKDLLA YKENGVIPTD    50
    KVLNGILSYL GMTKVELELK LGRIPAGLED VFLNNTKEIA KILENKNSVK 100
    LNEFNSIQEI KPYFYTDLGK LYNGDCLELF KQVPDENVDT IFADPPFNLD 150
    KEYDEGVTDK NSFSGYLDWY YKWIDECIRV LKPGGSLFIY NIPKWNTYLS 200
    EYLNRKLNFR NWITVDMKFG LPIQNRLYPA NYSLLYYVKG DKPKTFNVQR 250
    IPLQTCPHCG REIKDYGGYK NKMNPKGVTL SDVWSDIYPV RHSSSKNRKF 300
    NELSVKLLDR IITMSTNEGD VVLDPFGGSG TTFAVSEMLG RKWIGFELGN 350
    CEIIKERLKN KDKDKKLLGK VYEEKNKLFP NRVKELRKKN GLWIDDDFRQ 400
    DHEGNSKGDK KNENNDQISL SLE 423
    Length:423
    Mass (Da):49,136
    Last modified:September 21, 2011 - v3
    Checksum:i8C2D9F58F33E6669
    GO

    Sequence cautioni

    The sequence CAA38200.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X55285 Genomic DNA. Translation: CAA38998.2.
    X54303 Genomic DNA. Translation: CAA38200.1. Different initiation.
    PIRiS26843.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X55285 Genomic DNA. Translation: CAA38998.2 .
    X54303 Genomic DNA. Translation: CAA38200.1 . Different initiation.
    PIRi S26843.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    REBASEi 3293. M.BamHI.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    BRENDAi 2.1.1.113. 630.

    Family and domain databases

    Gene3Di 3.40.50.150. 1 hit.
    InterProi IPR002295. D21N6_MeTrfase.
    IPR002941. DNA_methylase_N4/N6.
    IPR002052. DNA_methylase_N6_adenine_CS.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view ]
    Pfami PF01555. N6_N4_Mtase. 1 hit.
    [Graphical view ]
    PRINTSi PR00506. D21N6MTFRASE.
    SUPFAMi SSF53335. SSF53335. 1 hit.
    PROSITEi PS00092. N6_MTASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Characterization of the cloned BamHI restriction modification system: its nucleotide sequence, properties of the methylase, and expression in heterologous hosts."
      Brooks J.E., Nathan P.D., Landry D., Sznyter L.A., White-Rees P., Ives C.L., Moran L.S., Slatko B.E., Benner J.S.
      Nucleic Acids Res. 19:841-850(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-19, FUNCTION.
      Strain: H.
    2. Benner J.S.
      Submitted (DEC-2010) to the EMBL/GenBank/DDBJ databases
      Cited for: SEQUENCE REVISION TO 28; 53; 167; 207 AND 348.
    3. "The complete sequence of the Bacillus amyloliquefaciens strain H, cellular BamHI methylase gene."
      Vanek P.G., Connaughton J.F., Kaloss W.D., Chirikjian J.G.
      Nucleic Acids Res. 18:6145-6145(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 24-423.
      Strain: H.

    Entry informationi

    Entry nameiMTB1_BACAM
    AccessioniPrimary (citable) accession number: P23941
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 1, 1992
    Last sequence update: September 21, 2011
    Last modified: October 1, 2014
    This is version 83 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing, Plasmid

    Documents

    1. Restriction enzymes and methylases
      Classification of restriction enzymes and methylases and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3