P23941 (MTB1_BACAM) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Modification methylase BamHI
Short name=M.BamHI
EC=2.1.1.113

Alternative name(s):
N(4)- cytosine-specific methyltransferase BamHI

Gene names

Name:

bamHIM

Encoded on

Plasmid

Organism

Bacillus amyloliquefaciens (Bacillus velezensis)

Taxonomic identifier

1390 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › Bacillus subtilis group

Protein attributes

Sequence length	423 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	This methylase recognizes the double-stranded sequence GGATCC, causes specific methylation on C-5 on both strands, and protects the DNA from cleavage by the BamHI endonuclease. Ref.1
Catalytic activity	S-adenosyl-L-methionine + DNA cytosine = S-adenosyl-L-homocysteine + DNA N(4)-methylcytosine.
Sequence similarities	Belongs to the N(4)/N(6)-methyltransferase family.
Sequence caution	The sequence CAA38200.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Ontologies

Keywords
Biological process	Restriction system
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Direct protein sequencing Plasmid
Gene Ontology (GO)
Biological_process	DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW N-4 methylation of cytosine Inferred from electronic annotation. Source: GOC
Molecular_function	DNA binding Inferred from electronic annotation. Source: InterPro N-methyltransferase activity Inferred from electronic annotation. Source: InterPro site-specific DNA-methyltransferase (cytosine-N4-specific) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 423	423	Modification methylase BamHI		PRO_0000087920

Sequences

	Sequence		Length	Mass (Da)
	P23941 [UniParc]. Last modified September 21, 2011. Version 3. Checksum: 8C2D9F58F33E6669	FASTA	423	49,136
	10 20 30 40 50 60 MRFFSVFDIV KNKANQLGYT ETEMYAVLKN YNVNKKDLLA YKENGVIPTD KVLNGILSYL 70 80 90 100 110 120 GMTKVELELK LGRIPAGLED VFLNNTKEIA KILENKNSVK LNEFNSIQEI KPYFYTDLGK 130 140 150 160 170 180 LYNGDCLELF KQVPDENVDT IFADPPFNLD KEYDEGVTDK NSFSGYLDWY YKWIDECIRV 190 200 210 220 230 240 LKPGGSLFIY NIPKWNTYLS EYLNRKLNFR NWITVDMKFG LPIQNRLYPA NYSLLYYVKG 250 260 270 280 290 300 DKPKTFNVQR IPLQTCPHCG REIKDYGGYK NKMNPKGVTL SDVWSDIYPV RHSSSKNRKF 310 320 330 340 350 360 NELSVKLLDR IITMSTNEGD VVLDPFGGSG TTFAVSEMLG RKWIGFELGN CEIIKERLKN 370 380 390 400 410 420 KDKDKKLLGK VYEEKNKLFP NRVKELRKKN GLWIDDDFRQ DHEGNSKGDK KNENNDQISL SLE

References

[1]	"Characterization of the cloned BamHI restriction modification system: its nucleotide sequence, properties of the methylase, and expression in heterologous hosts." Brooks J.E., Nathan P.D., Landry D., Sznyter L.A., White-Rees P., Ives C.L., Moran L.S., Slatko B.E., Benner J.S. Nucleic Acids Res. 19:841-850(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-19, FUNCTION. Strain: H.
[2]	Benner J.S. Submitted (DEC-2010) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION TO 28; 53; 167; 207 AND 348.
[3]	"The complete sequence of the Bacillus amyloliquefaciens strain H, cellular BamHI methylase gene." Vanek P.G., Connaughton J.F., Kaloss W.D., Chirikjian J.G. Nucleic Acids Res. 18:6145-6145(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 24-423. Strain: H.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X55285 Genomic DNA. Translation: CAA38998.2. X54303 Genomic DNA. Translation: CAA38200.1. Different initiation.
PIR	S26843.
3D structure databases
ModBase	Search...
Protein family/group databases
REBASE	3293. M.BamHI.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
BRENDA	2.1.1.113. 630.
Family and domain databases
InterPro	IPR002295. D21N6_MeTrfase. IPR002941. DNA_methylase_N4/N6. IPR002052. DNA_methylase_N6_adenine_CS. [Graphical view]
Pfam	PF01555. N6_N4_Mtase. 1 hit. [Graphical view]
PRINTS	PR00506. D21N6MTFRASE.
PROSITE	PS00092. N6_MTASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MTB1_BACAM

Accession

Primary (citable) accession number: P23941

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 1992
Last sequence update:	September 21, 2011
Last modified:	April 3, 2013
This is version 79 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program