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P23941 (MTB1_BACAM) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 81. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Modification methylase BamHI

Short name=M.BamHI
EC=2.1.1.113
Alternative name(s):
N(4)- cytosine-specific methyltransferase BamHI
Gene names
Name:bamHIM
Encoded onPlasmid
OrganismBacillus amyloliquefaciens (Bacillus velezensis)
Taxonomic identifier1390 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Protein attributes

Sequence length423 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

This methylase recognizes the double-stranded sequence GGATCC, causes specific methylation on C-5 on both strands, and protects the DNA from cleavage by the BamHI endonuclease. Ref.1

Catalytic activity

S-adenosyl-L-methionine + DNA cytosine = S-adenosyl-L-homocysteine + DNA N(4)-methylcytosine.

Sequence similarities

Belongs to the N(4)/N(6)-methyltransferase family.

Sequence caution

The sequence CAA38200.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 423423Modification methylase BamHI
PRO_0000087920

Sequences

Sequence LengthMass (Da)Tools
P23941 [UniParc].

Last modified September 21, 2011. Version 3.
Checksum: 8C2D9F58F33E6669

FASTA42349,136
        10         20         30         40         50         60 
MRFFSVFDIV KNKANQLGYT ETEMYAVLKN YNVNKKDLLA YKENGVIPTD KVLNGILSYL 

        70         80         90        100        110        120 
GMTKVELELK LGRIPAGLED VFLNNTKEIA KILENKNSVK LNEFNSIQEI KPYFYTDLGK 

       130        140        150        160        170        180 
LYNGDCLELF KQVPDENVDT IFADPPFNLD KEYDEGVTDK NSFSGYLDWY YKWIDECIRV 

       190        200        210        220        230        240 
LKPGGSLFIY NIPKWNTYLS EYLNRKLNFR NWITVDMKFG LPIQNRLYPA NYSLLYYVKG 

       250        260        270        280        290        300 
DKPKTFNVQR IPLQTCPHCG REIKDYGGYK NKMNPKGVTL SDVWSDIYPV RHSSSKNRKF 

       310        320        330        340        350        360 
NELSVKLLDR IITMSTNEGD VVLDPFGGSG TTFAVSEMLG RKWIGFELGN CEIIKERLKN 

       370        380        390        400        410        420 
KDKDKKLLGK VYEEKNKLFP NRVKELRKKN GLWIDDDFRQ DHEGNSKGDK KNENNDQISL 


SLE 

« Hide

References

[1]"Characterization of the cloned BamHI restriction modification system: its nucleotide sequence, properties of the methylase, and expression in heterologous hosts."
Brooks J.E., Nathan P.D., Landry D., Sznyter L.A., White-Rees P., Ives C.L., Moran L.S., Slatko B.E., Benner J.S.
Nucleic Acids Res. 19:841-850(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-19, FUNCTION.
Strain: H.
[2]Benner J.S.
Submitted (DEC-2010) to the EMBL/GenBank/DDBJ databases
Cited for: SEQUENCE REVISION TO 28; 53; 167; 207 AND 348.
[3]"The complete sequence of the Bacillus amyloliquefaciens strain H, cellular BamHI methylase gene."
Vanek P.G., Connaughton J.F., Kaloss W.D., Chirikjian J.G.
Nucleic Acids Res. 18:6145-6145(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 24-423.
Strain: H.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X55285 Genomic DNA. Translation: CAA38998.2.
X54303 Genomic DNA. Translation: CAA38200.1. Different initiation.
PIRS26843.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein family/group databases

REBASE3293. M.BamHI.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

BRENDA2.1.1.113. 630.

Family and domain databases

InterProIPR002295. D21N6_MeTrfase.
IPR002941. DNA_methylase_N4/N6.
IPR002052. DNA_methylase_N6_adenine_CS.
[Graphical view]
PfamPF01555. N6_N4_Mtase. 1 hit.
[Graphical view]
PRINTSPR00506. D21N6MTFRASE.
PROSITEPS00092. N6_MTASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMTB1_BACAM
AccessionPrimary (citable) accession number: P23941
Entry history
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: September 21, 2011
Last modified: April 16, 2014
This is version 81 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries