Reviewed, UniProtKB/Swiss-Prot P23941 (MTB1_BACAM)

Names and origin

Protein names

Recommended name:
    Modification methylase BamHI
      Short name=M.BamHI
    EC=2.1.1.113
Alternative name(s):
    N(4)- cytosine-specific methyltransferase BamHI

Gene names

Name:

bamHIM

Encoded on

Plasmid

Organism

Bacillus amyloliquefaciens

Taxonomic identifier

1390 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	423 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	This methylase recognizes the double-stranded sequence GGATCC, causes specific methylation on C-5 on both strands, and protects the DNA from cleavage by the BamHI endonuclease.
Catalytic activity	S-adenosyl-L-methionine + DNA cytosine = S-adenosyl-L-homocysteine + DNA N(4)-methylcytosine.
Sequence similarities	Belongs to the N(4)/N(6)-methyltransferase family.

Ontologies

Keywords
Biological process	Restriction system
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Direct protein sequencing Plasmid
Gene Ontology (GO)
Biological process	DNA methylation Inferred from electronic annotation. Source: InterPro DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro N-methyltransferase activity Inferred from electronic annotation. Source: InterPro site-specific DNA-methyltransferase (cytosine-N4-specific) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 423	423	Modification methylase BamHI		PRO_0000087920

Sequences

	Sequence		Length	Mass (Da)
	P23941-1 [UniParc]. Last modified November 1, 1995. Version 2. Checksum: 6C094F7B9E5339E7	FASTA	423	49,226
	10 20 30 40 50 60 MRFFSVFDIV KNKANQLGYT ETEMYAVMKN YNVNKKDLLA YKENGVIPTD KVMNGILSYL 70 80 90 100 110 120 GMTKVELELK LGRIPAGLED VFLNNTKEIA KILENKNSVK LNEFNSIQEI KPYFYTDLGK 130 140 150 160 170 180 LYNGDCLELF KQVPDENVDT IFADPPFNLD KEYDEGVTDK NSFSGYMDWY YKWIDECIRV 190 200 210 220 230 240 LKPGGSLFIY NIPKWNTYLS EYLNRKMNFR NWITVDMKFG LPIQNRLYPA NYSLLYYVKG 250 260 270 280 290 300 DKPKTFNVQR IPLQTCPHCG REIKDYGGYK NKMNPKGVTL SDVWSDIYPV RHSSSKNRKF 310 320 330 340 350 360 NELSVKLLDR IITMSTNEGD VVLDPFGGSG TTFAVSEMLG RKWIGFEMGN CEIIKERLKN 370 380 390 400 410 420 KDKDKKLLGK VYEEKNKLFP NRVKELRKKN GLWIDDDFRQ DHEGNSKGDK KNENNDQISL SLE

References

[1]

"Characterization of the cloned BamHI restriction modification system: its nucleotide sequence, properties of the methylase, and expression in heterologous hosts." Brooks J.E., Nathan P.D., Landry D., Sznyter L.A., White-Rees P., Ives C.L., Moran L.S., Slatko B.E., Benner J.S. Nucleic Acids Res. 19:841-850(1991) [PubMed: 1901989] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-19. Strain: 168.

[2]

"The complete sequence of the Bacillus amyloliquefaciens strain H, cellular BamHI methylase gene." Vanek P.G., Connaughton J.F., Kaloss W.D., Chirikjian J.G. Nucleic Acids Res. 18:6145-6145(1990) [PubMed: 2235511] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 24-423. Strain: H.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X55285 Genomic DNA. Translation: CAA38998.1. X54303 Genomic DNA. Translation: CAA38200.1.
PIR	S26843.
3D structure databases
HSSP	HSSP built from PDB template 1G60 based on UniProtKB P23192.
ModBase	Search...
Protein family/group databases
REBASE	3293. M.BamHI.
Enzyme and pathway databases
BRENDA	2.1.1.113. 1130.
Family and domain databases
InterPro	IPR002295. D21N6_MeTrfase. IPR002941. DNA_methylase_N4/N6. IPR002052. DNA_methylase_N6_adenine_CS. [Graphical view]
Pfam	PF01555. N6_N4_Mtase. 1 hit. [Graphical view]
PRINTS	PR00506. D21N6MTFRASE.
PROSITE	PS00092. N6_MTASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MTB1_BACAM

Accession

Primary (citable) accession number: P23941

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 1992
Last sequence update:	November 1, 1995
Last modified:	September 22, 2009
This is version 67 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)