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P23940

- T2BA_BACAM

UniProt

P23940 - T2BA_BACAM

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Protein

Type-2 restriction enzyme BamHI

Gene
bamHIR
Organism
Bacillus amyloliquefaciens (Bacillus velezensis)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Recognizes the double-stranded sequence GGATCC and cleaves after G-1.

Catalytic activityi

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Cofactori

Binds 2 magnesium ions per subunit.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi77 – 771Magnesium 2
Active sitei94 – 941
Metal bindingi94 – 941Magnesium 1
Metal bindingi94 – 941Magnesium 2
Active sitei111 – 1111
Metal bindingi112 – 1121Magnesium 1; via carbonyl oxygen
Active sitei113 – 1131

GO - Molecular functioni

  1. DNA binding Source: InterPro
  2. magnesium ion binding Source: InterPro
  3. Type II site-specific deoxyribonuclease activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

Restriction system

Keywords - Ligandi

Magnesium, Metal-binding

Protein family/group databases

REBASEi185. BamHI.

Names & Taxonomyi

Protein namesi
Recommended name:
Type-2 restriction enzyme BamHI (EC:3.1.21.4)
Short name:
R.BamHI
Alternative name(s):
Endonuclease BamHI
Type II restriction enzyme BamHI
Gene namesi
Name:bamHIR
Encoded oniPlasmid0 Publication
OrganismiBacillus amyloliquefaciens (Bacillus velezensis)
Taxonomic identifieri1390 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 213213Type-2 restriction enzyme BamHIPRO_0000077281Add
BLAST

Interactioni

Subunit structurei

Homodimer.

Structurei

Secondary structure

1
213
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi2 – 87
Helixi10 – 189
Helixi20 – 3415
Beta strandi43 – 486
Helixi58 – 614
Helixi62 – 709
Beta strandi75 – 784
Helixi81 – 844
Beta strandi87 – 893
Beta strandi93 – 1019
Beta strandi104 – 1129
Helixi117 – 13216
Beta strandi137 – 1448
Helixi146 – 1494
Helixi159 – 1624
Helixi163 – 1697
Beta strandi174 – 1807
Beta strandi182 – 1854
Helixi200 – 2056

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1BAMX-ray1.95A1-213[»]
1BHMX-ray2.20A/B1-213[»]
1ESGX-ray1.90A/B1-213[»]
2BAMX-ray2.00A/B1-213[»]
3BAMX-ray1.80A/B1-213[»]
ProteinModelPortaliP23940.
SMRiP23940. Positions 1-212.

Miscellaneous databases

EvolutionaryTraceiP23940.

Family & Domainsi

Family and domain databases

Gene3Di3.40.91.20. 1 hit.
InterProiIPR011338. BamHI/BglII/BstY.
IPR011335. Restrct_endonuc-II-like.
IPR004194. Restrct_endonuc_II_BamHI.
[Graphical view]
PfamiPF02923. BamHI. 1 hit.
[Graphical view]
PIRSFiPIRSF009309. Restrict_endonuc_II_BamHI. 1 hit.
SUPFAMiSSF52980. SSF52980. 1 hit.

Sequencei

Sequence statusi: Complete.

P23940-1 [UniParc]FASTAAdd to Basket

« Hide

MEVEKEFITD EAKELLSKDK LIQQAYNEVK TSICSPIWPA TSKTFTINNT    50
EKNCNGVVPI KELCYTLLED TYNWYREKPL DILKLEKKKG GPIDVYKEFI 100
ENSELKRVGM EFETGNISSA HRSMNKLLLG LKHGEIDLAI ILMPIKQLAY 150
YLTDRVTNFE ELEPYFELTE GQPFIFIGFN AEAYNSNVPL IPKGSDGMSK 200
RSIKKWKDKV ENK 213
Length:213
Mass (Da):24,570
Last modified:March 1, 1992 - v1
Checksum:iFFE57F518F37D8D6
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X55285 Genomic DNA. Translation: CAA38999.1.
PIRiA38693.
S26844.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X55285 Genomic DNA. Translation: CAA38999.1 .
PIRi A38693.
S26844.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1BAM X-ray 1.95 A 1-213 [» ]
1BHM X-ray 2.20 A/B 1-213 [» ]
1ESG X-ray 1.90 A/B 1-213 [» ]
2BAM X-ray 2.00 A/B 1-213 [» ]
3BAM X-ray 1.80 A/B 1-213 [» ]
ProteinModelPortali P23940.
SMRi P23940. Positions 1-212.
ModBasei Search...
MobiDBi Search...

Chemistry

BindingDBi P23940.
ChEMBLi CHEMBL5928.

Protein family/group databases

REBASEi 185. BamHI.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Miscellaneous databases

EvolutionaryTracei P23940.

Family and domain databases

Gene3Di 3.40.91.20. 1 hit.
InterProi IPR011338. BamHI/BglII/BstY.
IPR011335. Restrct_endonuc-II-like.
IPR004194. Restrct_endonuc_II_BamHI.
[Graphical view ]
Pfami PF02923. BamHI. 1 hit.
[Graphical view ]
PIRSFi PIRSF009309. Restrict_endonuc_II_BamHI. 1 hit.
SUPFAMi SSF52980. SSF52980. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Characterization of the cloned BamHI restriction modification system: its nucleotide sequence, properties of the methylase, and expression in heterologous hosts."
    Brooks J.E., Nathan P.D., Landry D., Sznyter L.A., White-Rees P., Ives C.L., Moran L.S., Slatko B.E., Benner J.S.
    Nucleic Acids Res. 19:841-850(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-8 AND 11-14.
    Strain: 168.
  2. "Structure of restriction endonuclease BamHI and its relationship to EcoRI."
    Newman M., Strzelecka T., Dorner L.F., Schildkraut I., Aggarwal A.K.
    Nature 368:660-664(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.95 ANGSTROMS).
  3. "The role of metals in catalysis by the restriction endonuclease BamHI."
    Viadiu H., Aggarwal A.K.
    Nat. Struct. Biol. 5:910-916(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS).

Entry informationi

Entry nameiT2BA_BACAM
AccessioniPrimary (citable) accession number: P23940
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: March 1, 1992
Last modified: April 16, 2014
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing, Plasmid

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries

External Data

Dasty 3

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