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P23940 (T2BA_BACAM) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 91. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Type-2 restriction enzyme BamHI

Short name=R.BamHI
EC=3.1.21.4
Alternative name(s):
Endonuclease BamHI
Type II restriction enzyme BamHI
Gene names
Name:bamHIR
Encoded onPlasmid
OrganismBacillus amyloliquefaciens (Bacillus velezensis)
Taxonomic identifier1390 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Protein attributes

Sequence length213 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Recognizes the double-stranded sequence GGATCC and cleaves after G-1.

Catalytic activity

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Cofactor

Binds 2 magnesium ions per subunit.

Subunit structure

Homodimer.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 213213Type-2 restriction enzyme BamHI
PRO_0000077281

Sites

Active site941
Active site1111
Active site1131
Metal binding771Magnesium 2
Metal binding941Magnesium 1
Metal binding941Magnesium 2
Metal binding1121Magnesium 1; via carbonyl oxygen

Secondary structure

..................................... 213
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P23940 [UniParc].

Last modified March 1, 1992. Version 1.
Checksum: FFE57F518F37D8D6

FASTA21324,570
        10         20         30         40         50         60 
MEVEKEFITD EAKELLSKDK LIQQAYNEVK TSICSPIWPA TSKTFTINNT EKNCNGVVPI 

        70         80         90        100        110        120 
KELCYTLLED TYNWYREKPL DILKLEKKKG GPIDVYKEFI ENSELKRVGM EFETGNISSA 

       130        140        150        160        170        180 
HRSMNKLLLG LKHGEIDLAI ILMPIKQLAY YLTDRVTNFE ELEPYFELTE GQPFIFIGFN 

       190        200        210 
AEAYNSNVPL IPKGSDGMSK RSIKKWKDKV ENK 

« Hide

References

[1]"Characterization of the cloned BamHI restriction modification system: its nucleotide sequence, properties of the methylase, and expression in heterologous hosts."
Brooks J.E., Nathan P.D., Landry D., Sznyter L.A., White-Rees P., Ives C.L., Moran L.S., Slatko B.E., Benner J.S.
Nucleic Acids Res. 19:841-850(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-8 AND 11-14.
Strain: 168.
[2]"Structure of restriction endonuclease BamHI and its relationship to EcoRI."
Newman M., Strzelecka T., Dorner L.F., Schildkraut I., Aggarwal A.K.
Nature 368:660-664(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.95 ANGSTROMS).
[3]"The role of metals in catalysis by the restriction endonuclease BamHI."
Viadiu H., Aggarwal A.K.
Nat. Struct. Biol. 5:910-916(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS).
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X55285 Genomic DNA. Translation: CAA38999.1.
PIRA38693.
S26844.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1BAMX-ray1.95A1-213[»]
1BHMX-ray2.20A/B1-213[»]
1ESGX-ray1.90A/B1-213[»]
2BAMX-ray2.00A/B1-213[»]
3BAMX-ray1.80A/B1-213[»]
ProteinModelPortalP23940.
SMRP23940. Positions 1-212.
ModBaseSearch...
MobiDBSearch...

Chemistry

BindingDBP23940.
ChEMBLCHEMBL5928.

Protein family/group databases

REBASE185. BamHI.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.40.91.20. 1 hit.
InterProIPR011338. BamHI/BglII/BstY.
IPR011335. Restrct_endonuc-II-like.
IPR004194. Restrct_endonuc_II_BamHI.
[Graphical view]
PfamPF02923. BamHI. 1 hit.
[Graphical view]
PIRSFPIRSF009309. Restrict_endonuc_II_BamHI. 1 hit.
SUPFAMSSF52980. SSF52980. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceP23940.

Entry information

Entry nameT2BA_BACAM
AccessionPrimary (citable) accession number: P23940
Entry history
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: March 1, 1992
Last modified: April 16, 2014
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries

PDB cross-references

Index of Protein Data Bank (PDB) cross-references