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P23940

- T2BA_BACAM

UniProt

P23940 - T2BA_BACAM

Protein

Type-2 restriction enzyme BamHI

Gene

bamHIR

Organism
Bacillus amyloliquefaciens (Bacillus velezensis)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 91 (16 Apr 2014)
      Sequence version 1 (01 Mar 1992)
      Previous versions | rss
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    Functioni

    Recognizes the double-stranded sequence GGATCC and cleaves after G-1.

    Catalytic activityi

    Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

    Cofactori

    Binds 2 magnesium ions per subunit.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi77 – 771Magnesium 2
    Active sitei94 – 941
    Metal bindingi94 – 941Magnesium 1
    Metal bindingi94 – 941Magnesium 2
    Active sitei111 – 1111
    Metal bindingi112 – 1121Magnesium 1; via carbonyl oxygen
    Active sitei113 – 1131

    GO - Molecular functioni

    1. DNA binding Source: InterPro
    2. magnesium ion binding Source: InterPro
    3. Type II site-specific deoxyribonuclease activity Source: UniProtKB-EC

    GO - Biological processi

    1. DNA restriction-modification system Source: UniProtKB-KW

    Keywords - Molecular functioni

    Endonuclease, Hydrolase, Nuclease

    Keywords - Biological processi

    Restriction system

    Keywords - Ligandi

    Magnesium, Metal-binding

    Protein family/group databases

    REBASEi185. BamHI.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Type-2 restriction enzyme BamHI (EC:3.1.21.4)
    Short name:
    R.BamHI
    Alternative name(s):
    Endonuclease BamHI
    Type II restriction enzyme BamHI
    Gene namesi
    Name:bamHIR
    Encoded oniPlasmid0 Publication
    OrganismiBacillus amyloliquefaciens (Bacillus velezensis)
    Taxonomic identifieri1390 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 213213Type-2 restriction enzyme BamHIPRO_0000077281Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.

    Structurei

    Secondary structure

    1
    213
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi2 – 87
    Helixi10 – 189
    Helixi20 – 3415
    Beta strandi43 – 486
    Helixi58 – 614
    Helixi62 – 709
    Beta strandi75 – 784
    Helixi81 – 844
    Beta strandi87 – 893
    Beta strandi93 – 1019
    Beta strandi104 – 1129
    Helixi117 – 13216
    Beta strandi137 – 1448
    Helixi146 – 1494
    Helixi159 – 1624
    Helixi163 – 1697
    Beta strandi174 – 1807
    Beta strandi182 – 1854
    Helixi200 – 2056

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1BAMX-ray1.95A1-213[»]
    1BHMX-ray2.20A/B1-213[»]
    1ESGX-ray1.90A/B1-213[»]
    2BAMX-ray2.00A/B1-213[»]
    3BAMX-ray1.80A/B1-213[»]
    ProteinModelPortaliP23940.
    SMRiP23940. Positions 1-212.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP23940.

    Family & Domainsi

    Family and domain databases

    Gene3Di3.40.91.20. 1 hit.
    InterProiIPR011338. BamHI/BglII/BstY.
    IPR011335. Restrct_endonuc-II-like.
    IPR004194. Restrct_endonuc_II_BamHI.
    [Graphical view]
    PfamiPF02923. BamHI. 1 hit.
    [Graphical view]
    PIRSFiPIRSF009309. Restrict_endonuc_II_BamHI. 1 hit.
    SUPFAMiSSF52980. SSF52980. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P23940-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEVEKEFITD EAKELLSKDK LIQQAYNEVK TSICSPIWPA TSKTFTINNT    50
    EKNCNGVVPI KELCYTLLED TYNWYREKPL DILKLEKKKG GPIDVYKEFI 100
    ENSELKRVGM EFETGNISSA HRSMNKLLLG LKHGEIDLAI ILMPIKQLAY 150
    YLTDRVTNFE ELEPYFELTE GQPFIFIGFN AEAYNSNVPL IPKGSDGMSK 200
    RSIKKWKDKV ENK 213
    Length:213
    Mass (Da):24,570
    Last modified:March 1, 1992 - v1
    Checksum:iFFE57F518F37D8D6
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X55285 Genomic DNA. Translation: CAA38999.1.
    PIRiA38693.
    S26844.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X55285 Genomic DNA. Translation: CAA38999.1 .
    PIRi A38693.
    S26844.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1BAM X-ray 1.95 A 1-213 [» ]
    1BHM X-ray 2.20 A/B 1-213 [» ]
    1ESG X-ray 1.90 A/B 1-213 [» ]
    2BAM X-ray 2.00 A/B 1-213 [» ]
    3BAM X-ray 1.80 A/B 1-213 [» ]
    ProteinModelPortali P23940.
    SMRi P23940. Positions 1-212.
    ModBasei Search...
    MobiDBi Search...

    Chemistry

    BindingDBi P23940.
    ChEMBLi CHEMBL5928.

    Protein family/group databases

    REBASEi 185. BamHI.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Miscellaneous databases

    EvolutionaryTracei P23940.

    Family and domain databases

    Gene3Di 3.40.91.20. 1 hit.
    InterProi IPR011338. BamHI/BglII/BstY.
    IPR011335. Restrct_endonuc-II-like.
    IPR004194. Restrct_endonuc_II_BamHI.
    [Graphical view ]
    Pfami PF02923. BamHI. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF009309. Restrict_endonuc_II_BamHI. 1 hit.
    SUPFAMi SSF52980. SSF52980. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Characterization of the cloned BamHI restriction modification system: its nucleotide sequence, properties of the methylase, and expression in heterologous hosts."
      Brooks J.E., Nathan P.D., Landry D., Sznyter L.A., White-Rees P., Ives C.L., Moran L.S., Slatko B.E., Benner J.S.
      Nucleic Acids Res. 19:841-850(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-8 AND 11-14.
      Strain: 168.
    2. "Structure of restriction endonuclease BamHI and its relationship to EcoRI."
      Newman M., Strzelecka T., Dorner L.F., Schildkraut I., Aggarwal A.K.
      Nature 368:660-664(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.95 ANGSTROMS).
    3. "The role of metals in catalysis by the restriction endonuclease BamHI."
      Viadiu H., Aggarwal A.K.
      Nat. Struct. Biol. 5:910-916(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS).

    Entry informationi

    Entry nameiT2BA_BACAM
    AccessioniPrimary (citable) accession number: P23940
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 1, 1992
    Last sequence update: March 1, 1992
    Last modified: April 16, 2014
    This is version 91 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Direct protein sequencing, Plasmid

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. Restriction enzymes and methylases
      Classification of restriction enzymes and methylases and list of entries

    External Data

    Dasty 3