Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Apolipoprotein N-acyltransferase

Gene

lnt

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Transfers the fatty acyl group on membrane lipoproteins.

Pathwayi: lipoprotein biosynthesis (N-acyl transfer)

This protein is involved in the pathway lipoprotein biosynthesis (N-acyl transfer), which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway lipoprotein biosynthesis (N-acyl transfer) and in Protein modification.

GO - Molecular functioni

GO - Biological processi

  • lipoprotein biosynthetic process Source: EcoCyc
  • nitrogen compound metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Ligandi

Copper

Enzyme and pathway databases

BioCyciEcoCyc:EG10168-MONOMER.
ECOL316407:JW0654-MONOMER.
MetaCyc:EG10168-MONOMER.
UniPathwayiUPA00666.

Names & Taxonomyi

Protein namesi
Recommended name:
Apolipoprotein N-acyltransferase (EC:2.3.1.-)
Short name:
ALP N-acyltransferase
Alternative name(s):
Copper homeostasis protein CutE
Gene namesi
Name:lnt
Synonyms:cutE
Ordered Locus Names:b0657, JW0654
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10168. cutE.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei12 – 28HelicalSequence analysisAdd BLAST17
Transmembranei57 – 77HelicalSequence analysisAdd BLAST21
Transmembranei91 – 111HelicalSequence analysisAdd BLAST21
Transmembranei168 – 188HelicalSequence analysisAdd BLAST21
Transmembranei194 – 214HelicalSequence analysisAdd BLAST21
Transmembranei487 – 507HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

  • integral component of plasma membrane Source: EcoCyc
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001780621 – 512Apolipoprotein N-acyltransferaseAdd BLAST512

Proteomic databases

PaxDbiP23930.
PRIDEiP23930.

Interactioni

Protein-protein interaction databases

BioGridi4259916. 191 interactors.
DIPiDIP-10111N.
IntActiP23930. 2 interactors.
MINTiMINT-1238661.
STRINGi511145.b0657.

Structurei

3D structure databases

ProteinModelPortaliP23930.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini222 – 512CN hydrolaseAdd BLAST291

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni425 – 431Could contain a copper-binding motif7

Sequence similaritiesi

Contains 1 CN hydrolase domain.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105CE8. Bacteria.
COG0815. LUCA.
HOGENOMiHOG000264279.
InParanoidiP23930.
KOiK03820.
OMAiIPQSMKW.
PhylomeDBiP23930.

Family and domain databases

CDDicd07571. ALP_N-acyl_transferase. 1 hit.
Gene3Di3.60.110.10. 1 hit.
HAMAPiMF_01148. Lnt. 1 hit.
InterProiIPR004563. Apolipo_AcylTrfase.
IPR003010. C-N_Hydrolase.
[Graphical view]
PfamiPF00795. CN_hydrolase. 1 hit.
[Graphical view]
SUPFAMiSSF56317. SSF56317. 1 hit.
TIGRFAMsiTIGR00546. lnt. 1 hit.
PROSITEiPS50263. CN_HYDROLASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P23930-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAFASLIERQ RIRLLLALLF GACGTLAFSP YDVWPAAIIS LMGLQALTFN
60 70 80 90 100
RRPLQSAAIG FCWGFGLFGS GINWVYVSIA TFGGMPGPVN IFLVVLLAAY
110 120 130 140 150
LSLYTGLFAG VLSRLWPKTT WLRVAIAAPA LWQVTEFLRG WVLTGFPWLQ
160 170 180 190 200
FGYSQIDGPL KGLAPIMGVE AINFLLMMVS GLLALALVKR NWRPLVVAVV
210 220 230 240 250
LFALPFPLRY IQWFTPQPEK TIQVSMVQGD IPQSLKWDEG QLLNTLKIYY
260 270 280 290 300
NATAPLMGKS SLIIWPESAI TDLEINQQPF LKALDGELRD KGSSLVTGIV
310 320 330 340 350
DARLNKQNRY DTYNTIITLG KGAPYSYESA DRYNKNHLVP FGEFVPLESI
360 370 380 390 400
LRPLAPFFDL PMSSFSRGPY IQPPLSANGI ELTAAICYEI ILGEQVRDNF
410 420 430 440 450
RPDTDYLLTI SNDAWFGKSI GPWQHFQMAR MRALELARPL LRSTNNGITA
460 470 480 490 500
VIGPQGEIQA MIPQFTREVL TTNVTPTTGL TPYARTGNWP LWVLTALFGF
510
AAVLMSLRQR RK
Length:512
Mass (Da):57,066
Last modified:March 1, 1992 - v1
Checksum:i70CFD6627C286615
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti186A → V (PubMed:8905232).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X58070 Genomic DNA. Translation: CAA41100.1.
U82598 Genomic DNA. Translation: AAB40859.1.
U00096 Genomic DNA. Translation: AAC73758.1.
AP009048 Genomic DNA. Translation: BAA35308.2.
PIRiS18194.
RefSeqiNP_415190.1. NC_000913.3.
WP_000853021.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73758; AAC73758; b0657.
BAA35308; BAA35308; BAA35308.
GeneIDi946201.
KEGGiecj:JW0654.
eco:b0657.
PATRICi32116503. VBIEscCol129921_0691.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X58070 Genomic DNA. Translation: CAA41100.1.
U82598 Genomic DNA. Translation: AAB40859.1.
U00096 Genomic DNA. Translation: AAC73758.1.
AP009048 Genomic DNA. Translation: BAA35308.2.
PIRiS18194.
RefSeqiNP_415190.1. NC_000913.3.
WP_000853021.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP23930.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259916. 191 interactors.
DIPiDIP-10111N.
IntActiP23930. 2 interactors.
MINTiMINT-1238661.
STRINGi511145.b0657.

Proteomic databases

PaxDbiP23930.
PRIDEiP23930.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73758; AAC73758; b0657.
BAA35308; BAA35308; BAA35308.
GeneIDi946201.
KEGGiecj:JW0654.
eco:b0657.
PATRICi32116503. VBIEscCol129921_0691.

Organism-specific databases

EchoBASEiEB0166.
EcoGeneiEG10168. cutE.

Phylogenomic databases

eggNOGiENOG4105CE8. Bacteria.
COG0815. LUCA.
HOGENOMiHOG000264279.
InParanoidiP23930.
KOiK03820.
OMAiIPQSMKW.
PhylomeDBiP23930.

Enzyme and pathway databases

UniPathwayiUPA00666.
BioCyciEcoCyc:EG10168-MONOMER.
ECOL316407:JW0654-MONOMER.
MetaCyc:EG10168-MONOMER.

Miscellaneous databases

PROiP23930.

Family and domain databases

CDDicd07571. ALP_N-acyl_transferase. 1 hit.
Gene3Di3.60.110.10. 1 hit.
HAMAPiMF_01148. Lnt. 1 hit.
InterProiIPR004563. Apolipo_AcylTrfase.
IPR003010. C-N_Hydrolase.
[Graphical view]
PfamiPF00795. CN_hydrolase. 1 hit.
[Graphical view]
SUPFAMiSSF56317. SSF56317. 1 hit.
TIGRFAMsiTIGR00546. lnt. 1 hit.
PROSITEiPS50263. CN_HYDROLASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLNT_ECOLI
AccessioniPrimary (citable) accession number: P23930
Secondary accession number(s): P77703
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: March 1, 1992
Last modified: November 2, 2016
This is version 137 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.