Reviewed, UniProtKB/Swiss-Prot P23920 (SYM_BACST)

Names and origin

Protein names

Recommended name:
    Methionyl-tRNA synthetase
    EC=6.1.1.10
Alternative name(s):
    Methionine--tRNA ligase
      Short name=MetRS

Gene names

Name:	metG
Synonyms:	metS

Organism

Bacillus stearothermophilus (Geobacillus stearothermophilus)

Taxonomic identifier

1422 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Geobacillus

Protein attributes

Sequence length	649 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation By similarity.
Catalytic activity	ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met). HAMAP MF_01228
Cofactor	Binds 1 zinc ion per subunit. HAMAP MF_01228
Subunit structure	Homodimer. HAMAP MF_01228
Subcellular location	Cytoplasm. HAMAP MF_01228
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 2A subfamily. Contains 1 tRNA-binding domain.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding RNA-binding Zinc tRNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Gene Ontology (GO)
Biological process	methionyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP methionine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP tRNA binding Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 649	649	Methionyl-tRNA synthetase HAMAP MF_01228		PRO_0000139210
Regions
	Domain	555 – 647	93	tRNA-binding
	Motif	13 – 23	11	"HIGH" region HAMAP MF_01228
	Motif	298 – 302	5	"KMSKS" region HAMAP MF_01228
Sites
	Metal binding	128	1	Zinc By similarity
	Metal binding	131	1	Zinc By similarity
	Metal binding	145	1	Zinc By similarity
	Metal binding	148	1	Zinc By similarity
	Binding site	301	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	P23920-1 [UniParc]. Last modified March 1, 1992. Version 1. Checksum: BDF5DCCA79B21A89	FASTA	649	74,354
	10 20 30 40 50 60 MEKKTFYLTT PIYYPSDKLH IGHAYTTVAG DAMARYKRLR GYDVMYLTGT DEHGQKIQRK 70 80 90 100 110 120 AQEKGVTPQQ YVDDIVAGIQ ELWRKLDISY DDFIRTTQER HKKIVEKIFA RLVEQGDIYL 130 140 150 160 170 180 GEYEGWYCTP CESFYTERQL VDGNCPDCGR PVEKVKEESY FFRMSKYVDR LLQYYEENPD 190 200 210 220 230 240 FIQPESRKNE MINNFIKPGL EDLAVSRTTF DWGIKVPGDP KHVIYVWIDA LANYITALGY 250 260 270 280 290 300 GTDNDEKFRK YWPADVHLVG KEIVRFHTIY WPIMLMALGL PLPKKVFGHG WLLMKDGKMS 310 320 330 340 350 360 KSKGNVVDPV MIIDRYGLDA LRYYLLREVP FGSDGVFTPE GFIERINYDL ANDLGNLLHR 370 380 390 400 410 420 TVAMIEKYFG GAIPPYRGPK TPFDRDLSET AREVVRQYEE AMERMEFSVA LSAVWQLIGR 430 440 450 460 470 480 TNKYIDETQP WVLAKEESKR EELASVMAHL AESLRYTAVL LQPFLTRTPE RIFTQLGISD 490 500 510 520 530 540 RSLKEWDSLY DFGLIPEGTN VQKGEPLFPR LDIGVEVEYI KAHMQGGKPA EAAKEEKQAA 550 560 570 580 590 600 RAEEISIDDF AKVDLRVAEV VQPERMKNAD KLLKLQLDLG GEKRQVISGI AEFYKPEELI 610 620 630 640 GKKVICVANL KPAKLRGEWS EGMILAGGSG GGFSLATVDQ HVPNGTKIK

References

[1]

"Methionyl-tRNA synthetase from Bacillus stearothermophilus: structural and functional identities with the Escherichia coli enzyme." Mechulam Y., Schmitt E., Panvert M., Schmitter J.-M., Lapadat-Tapolsky M., Meinnel T., Dessen P., Blanquet S., Fayat G. Nucleic Acids Res. 19:3673-3681(1991) [PubMed: 1852609] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 1518.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X57925 Genomic DNA. Translation: CAA40999.1.
PIR	S16682.
3D structure databases
HSSP	HSSP built from PDB template 1MKH based on UniProtKB Q9V011.
ModBase	Search...
Enzyme and pathway databases
BRENDA	6.1.1.10. 266715.
Family and domain databases
HAMAP	MF_01228. Fused. [Tree]
InterPro	IPR015413. aa-tRNA-synt_I. IPR001412. aa-tRNA-synth_I_CS. IPR002304. Met-tRNA-synth_Ia. IPR004495. Met-tRNA-synth_Ia_bsu_C. IPR012340. NA-bd_OB-fold. IPR014729. Rossmann-like_a/b/a_fold. IPR014758. tRNA-synt_met_N. IPR002547. tRNA_bd. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
Pfam	PF09334. tRNA-synt_1g. 1 hit. PF01588. tRNA_bind. 1 hit. [Graphical view]
PRINTS	PR01041. TRNASYNTHMET.
TIGRFAMs	TIGR00398. metG. 1 hit. TIGR00399. metG_C_term. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. PS50886. TRBD. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYM_BACST

Accession

Primary (citable) accession number: P23920

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 1992
Last sequence update:	March 1, 1992
Last modified:	June 16, 2009
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)