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P23919

- KTHY_HUMAN

UniProt

P23919 - KTHY_HUMAN

Protein

Thymidylate kinase

Gene

DTYMK

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 141 (01 Oct 2014)
      Sequence version 4 (07 Dec 2004)
      Previous versions | rss
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    Functioni

    Catalyzes the conversion of dTMP to dTDP.

    Catalytic activityi

    ATP + dTMP = ADP + dTDP.

    Pathwayi

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi13 – 208ATPCurated

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. nucleoside phosphate kinase activity Source: Reactome
    3. thymidylate kinase activity Source: MGI

    GO - Biological processi

    1. cell cycle Source: ProtInc
    2. cell proliferation Source: ProtInc
    3. cellular response to growth factor stimulus Source: Ensembl
    4. dTDP biosynthetic process Source: Ensembl
    5. dTTP biosynthetic process Source: UniProtKB-UniPathway
    6. myoblast differentiation Source: Ensembl
    7. nucleobase-containing small molecule interconversion Source: Reactome
    8. nucleobase-containing small molecule metabolic process Source: Reactome
    9. nucleoside monophosphate phosphorylation Source: GOC
    10. nucleotide phosphorylation Source: GOC
    11. response to cadmium ion Source: Ensembl
    12. response to estrogen Source: Ensembl
    13. small molecule metabolic process Source: Reactome

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Nucleotide biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciMetaCyc:HS11384-MONOMER.
    ReactomeiREACT_21330. Synthesis and interconversion of nucleotide di- and triphosphates.
    SABIO-RKP23919.
    UniPathwayiUPA00575.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Thymidylate kinase (EC:2.7.4.9)
    Alternative name(s):
    dTMP kinase
    Gene namesi
    Name:DTYMK
    Synonyms:CDC8, TMPK, TYMK
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 2

    Organism-specific databases

    HGNCiHGNC:3061. DTYMK.

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: Reactome
    2. mitochondrial intermembrane space Source: Ensembl
    3. mitochondrial matrix Source: Ensembl

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA150.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 212211Thymidylate kinasePRO_0000155210Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylalanine1 Publication
    Modified residuei169 – 1691N6-acetyllysine1 Publication

    Keywords - PTMi

    Acetylation

    Proteomic databases

    MaxQBiP23919.
    PaxDbiP23919.
    PRIDEiP23919.

    PTM databases

    PhosphoSiteiP23919.

    Expressioni

    Gene expression databases

    ArrayExpressiP23919.
    BgeeiP23919.
    CleanExiHS_DTYMK.
    GenevestigatoriP23919.

    Organism-specific databases

    HPAiHPA042593.
    HPA042719.

    Interactioni

    Protein-protein interaction databases

    BioGridi108174. 5 interactions.
    IntActiP23919. 2 interactions.
    MINTiMINT-3010142.
    STRINGi9606.ENSP00000304802.

    Structurei

    Secondary structure

    1
    212
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi8 – 147
    Helixi19 – 3214
    Beta strandi37 – 437
    Helixi48 – 5710
    Helixi65 – 7713
    Helixi80 – 889
    Beta strandi92 – 976
    Helixi99 – 1079
    Helixi114 – 1185
    Helixi119 – 1213
    Beta strandi124 – 1263
    Beta strandi128 – 1347
    Helixi137 – 1426
    Helixi149 – 1513
    Helixi154 – 16714
    Beta strandi171 – 1733
    Beta strandi175 – 1795
    Helixi184 – 20118
    Helixi202 – 2043

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1E2DX-ray1.65A1-212[»]
    1E2EX-ray2.00A1-212[»]
    1E2FX-ray1.60A1-212[»]
    1E2GX-ray1.70A1-212[»]
    1E2QX-ray1.70A1-212[»]
    1E98X-ray1.90A1-212[»]
    1E99X-ray1.80A1-212[»]
    1E9AX-ray1.60A1-212[»]
    1E9BX-ray1.70A1-212[»]
    1E9CX-ray1.60A1-212[»]
    1E9DX-ray1.70A1-212[»]
    1E9EX-ray1.60A1-212[»]
    1E9FX-ray1.90A1-144[»]
    A149-212[»]
    1NMXX-ray1.70A1-212[»]
    1NMYX-ray1.60A4-212[»]
    1NMZX-ray1.75A1-212[»]
    1NN0X-ray1.60A1-212[»]
    1NN1X-ray1.90A1-212[»]
    1NN3X-ray1.55A1-212[»]
    1NN5X-ray1.50A1-212[»]
    2XX3X-ray2.00A1-212[»]
    ProteinModelPortaliP23919.
    SMRiP23919. Positions 4-212.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP23919.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the thymidylate kinase family.Curated

    Phylogenomic databases

    eggNOGiCOG0125.
    HOGENOMiHOG000229079.
    HOVERGENiHBG051418.
    InParanoidiP23919.
    KOiK00943.
    OMAiGKMIDSY.
    OrthoDBiEOG7WQ7T8.
    PhylomeDBiP23919.
    TreeFamiTF324638.

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    HAMAPiMF_00165. Thymidylate_kinase.
    InterProiIPR027417. P-loop_NTPase.
    IPR018095. Thymidylate_kin_CS.
    IPR018094. Thymidylate_kinase.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.
    TIGRFAMsiTIGR00041. DTMP_kinase. 1 hit.
    PROSITEiPS01331. THYMIDYLATE_KINASE. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: P23919-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MAARRGALIV LEGVDRAGKS TQSRKLVEAL CAAGHRAELL RFPERSTEIG    50
    KLLSSYLQKK SDVEDHSVHL LFSANRWEQV PLIKEKLSQG VTLVVDRYAF 100
    SGVAFTGAKE NFSLDWCKQP DVGLPKPDLV LFLQLQLADA AKRGAFGHER 150
    YENGAFQERA LRCFHQLMKD TTLNWKMVDA SKSIEAVHED IRVLSEDAIR 200
    TATEKPLGEL WK 212
    Length:212
    Mass (Da):23,819
    Last modified:December 7, 2004 - v4
    Checksum:iA52876625B3621B1
    GO
    Isoform 2 (identifier: P23919-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         111-134: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:188
    Mass (Da):21,064
    Checksum:i38FE4F99F6DDA886
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti3 – 31A → P in CAG46783. 1 PublicationCurated
    Sequence conflicti31 – 377CAAGHRA → SRGPPP in CAA38528. (PubMed:2017365)Curated
    Sequence conflicti58 – 581Q → K in AAA21719. (PubMed:8024690)Curated
    Sequence conflicti68 – 681V → A in CAG46783. 1 PublicationCurated
    Sequence conflicti183 – 1842SI → RL in CAA38528. (PubMed:2017365)Curated
    Sequence conflicti190 – 1912DI → EL(PubMed:2017365)Curated
    Sequence conflicti190 – 1912DI → EL(PubMed:8024690)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei111 – 13424Missing in isoform 2. 1 PublicationVSP_054164Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X54729 mRNA. Translation: CAA38528.1.
    L16991 mRNA. Translation: AAA21719.1.
    CR541986 mRNA. Translation: CAG46783.1.
    BC001827 mRNA. Translation: AAH01827.1.
    BC171902 mRNA. Translation: AAI71902.1.
    CCDSiCCDS2552.1. [P23919-1]
    PIRiS26845.
    RefSeqiNP_001158503.1. NM_001165031.1. [P23919-2]
    NP_036277.2. NM_012145.3. [P23919-1]
    UniGeneiHs.471873.

    Genome annotation databases

    EnsembliENST00000305784; ENSP00000304802; ENSG00000168393. [P23919-1]
    GeneIDi1841.
    KEGGihsa:1841.
    UCSCiuc002wbz.2. human. [P23919-1]

    Polymorphism databases

    DMDMi56405325.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X54729 mRNA. Translation: CAA38528.1 .
    L16991 mRNA. Translation: AAA21719.1 .
    CR541986 mRNA. Translation: CAG46783.1 .
    BC001827 mRNA. Translation: AAH01827.1 .
    BC171902 mRNA. Translation: AAI71902.1 .
    CCDSi CCDS2552.1. [P23919-1 ]
    PIRi S26845.
    RefSeqi NP_001158503.1. NM_001165031.1. [P23919-2 ]
    NP_036277.2. NM_012145.3. [P23919-1 ]
    UniGenei Hs.471873.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1E2D X-ray 1.65 A 1-212 [» ]
    1E2E X-ray 2.00 A 1-212 [» ]
    1E2F X-ray 1.60 A 1-212 [» ]
    1E2G X-ray 1.70 A 1-212 [» ]
    1E2Q X-ray 1.70 A 1-212 [» ]
    1E98 X-ray 1.90 A 1-212 [» ]
    1E99 X-ray 1.80 A 1-212 [» ]
    1E9A X-ray 1.60 A 1-212 [» ]
    1E9B X-ray 1.70 A 1-212 [» ]
    1E9C X-ray 1.60 A 1-212 [» ]
    1E9D X-ray 1.70 A 1-212 [» ]
    1E9E X-ray 1.60 A 1-212 [» ]
    1E9F X-ray 1.90 A 1-144 [» ]
    A 149-212 [» ]
    1NMX X-ray 1.70 A 1-212 [» ]
    1NMY X-ray 1.60 A 4-212 [» ]
    1NMZ X-ray 1.75 A 1-212 [» ]
    1NN0 X-ray 1.60 A 1-212 [» ]
    1NN1 X-ray 1.90 A 1-212 [» ]
    1NN3 X-ray 1.55 A 1-212 [» ]
    1NN5 X-ray 1.50 A 1-212 [» ]
    2XX3 X-ray 2.00 A 1-212 [» ]
    ProteinModelPortali P23919.
    SMRi P23919. Positions 4-212.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 108174. 5 interactions.
    IntActi P23919. 2 interactions.
    MINTi MINT-3010142.
    STRINGi 9606.ENSP00000304802.

    Chemistry

    BindingDBi P23919.
    ChEMBLi CHEMBL4388.

    PTM databases

    PhosphoSitei P23919.

    Polymorphism databases

    DMDMi 56405325.

    Proteomic databases

    MaxQBi P23919.
    PaxDbi P23919.
    PRIDEi P23919.

    Protocols and materials databases

    DNASUi 1841.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000305784 ; ENSP00000304802 ; ENSG00000168393 . [P23919-1 ]
    GeneIDi 1841.
    KEGGi hsa:1841.
    UCSCi uc002wbz.2. human. [P23919-1 ]

    Organism-specific databases

    CTDi 1841.
    GeneCardsi GC02M242634.
    H-InvDB HIX0161852.
    HGNCi HGNC:3061. DTYMK.
    HPAi HPA042593.
    HPA042719.
    MIMi 188345. gene.
    neXtProti NX_P23919.
    PharmGKBi PA150.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG0125.
    HOGENOMi HOG000229079.
    HOVERGENi HBG051418.
    InParanoidi P23919.
    KOi K00943.
    OMAi GKMIDSY.
    OrthoDBi EOG7WQ7T8.
    PhylomeDBi P23919.
    TreeFami TF324638.

    Enzyme and pathway databases

    UniPathwayi UPA00575 .
    BioCyci MetaCyc:HS11384-MONOMER.
    Reactomei REACT_21330. Synthesis and interconversion of nucleotide di- and triphosphates.
    SABIO-RK P23919.

    Miscellaneous databases

    EvolutionaryTracei P23919.
    GeneWikii DTYMK.
    GenomeRNAii 1841.
    NextBioi 7537.
    PROi P23919.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P23919.
    Bgeei P23919.
    CleanExi HS_DTYMK.
    Genevestigatori P23919.

    Family and domain databases

    Gene3Di 3.40.50.300. 1 hit.
    HAMAPi MF_00165. Thymidylate_kinase.
    InterProi IPR027417. P-loop_NTPase.
    IPR018095. Thymidylate_kin_CS.
    IPR018094. Thymidylate_kinase.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 1 hit.
    TIGRFAMsi TIGR00041. DTMP_kinase. 1 hit.
    PROSITEi PS01331. THYMIDYLATE_KINASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning and expression of the human deoxythymidylate kinase gene in yeast."
      Su J.Y., Sclafani R.A.
      Nucleic Acids Res. 19:823-827(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    2. "Human dTMP kinase: gene expression and enzymatic activity coinciding with cell cycle progression and cell growth."
      Huang S.H., Tang A., Drisco B., Zhang S.Q., Seeger R., Li C., Jong A.
      DNA Cell Biol. 13:461-471(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    3. "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
      Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S., Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W., Korn B., Zuo D., Hu Y., LaBaer J.
      Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
      Tissue: Brain and Muscle.
    5. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
      Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
      Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
    6. "Lysine acetylation targets protein complexes and co-regulates major cellular functions."
      Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.C., Olsen J.V., Mann M.
      Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-169, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    8. "Structures of human thymidylate kinase in complex with prodrugs: implications for the structure-based design of novel compounds."
      Ostermann N., Segura-Pena D., Meier C., Veit T., Monnerjahn C., Konrad M., Lavie A.
      Biochemistry 42:2568-2577(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.65 ANGSTROMS).

    Entry informationi

    Entry nameiKTHY_HUMAN
    AccessioniPrimary (citable) accession number: P23919
    Secondary accession number(s): B7ZW70, Q6FGX1, Q9BUX4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 1, 1992
    Last sequence update: December 7, 2004
    Last modified: October 1, 2014
    This is version 141 of the entry and version 4 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Human chromosome 2
      Human chromosome 2: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    4. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    5. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3