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Protein

Thymidylate kinase

Gene

DTYMK

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the conversion of dTMP to dTDP.

Catalytic activityi

ATP + dTMP = ADP + dTDP.

Pathwayi: dTTP biosynthesis

This protein is involved in the pathway dTTP biosynthesis, which is part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the pathway dTTP biosynthesis and in Pyrimidine metabolism.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi13 – 20ATPCurated8

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Nucleotide biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:HS11626-MONOMER.
ZFISH:HS11626-MONOMER.
BRENDAi2.7.4.9. 2681.
ReactomeiR-HSA-499943. Synthesis and interconversion of nucleotide di- and triphosphates.
SABIO-RKP23919.
UniPathwayiUPA00575.

Names & Taxonomyi

Protein namesi
Recommended name:
Thymidylate kinase (EC:2.7.4.9)
Alternative name(s):
dTMP kinase
Gene namesi
Name:DTYMK
Synonyms:CDC8, TMPK, TYMK
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 2

Organism-specific databases

HGNCiHGNC:3061. DTYMK.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Organism-specific databases

DisGeNETi1841.
OpenTargetsiENSG00000168393.
PharmGKBiPA150.

Chemistry databases

ChEMBLiCHEMBL4388.

Polymorphism and mutation databases

BioMutaiDTYMK.
DMDMi56405325.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00001552102 – 212Thymidylate kinaseAdd BLAST211

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1
Modified residuei169N6-acetyllysineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiP23919.
PaxDbiP23919.
PeptideAtlasiP23919.
PRIDEiP23919.

PTM databases

iPTMnetiP23919.
PhosphoSitePlusiP23919.
SwissPalmiP23919.

Expressioni

Gene expression databases

BgeeiENSG00000168393.
CleanExiHS_DTYMK.
ExpressionAtlasiP23919. baseline and differential.
GenevisibleiP23919. HS.

Organism-specific databases

HPAiHPA042593.
HPA042719.

Interactioni

Protein-protein interaction databases

BioGridi108174. 8 interactors.
IntActiP23919. 3 interactors.
MINTiMINT-3010142.
STRINGi9606.ENSP00000304802.

Chemistry databases

BindingDBiP23919.

Structurei

Secondary structure

1212
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi8 – 14Combined sources7
Helixi19 – 32Combined sources14
Beta strandi37 – 43Combined sources7
Helixi48 – 57Combined sources10
Helixi65 – 77Combined sources13
Helixi80 – 88Combined sources9
Beta strandi92 – 97Combined sources6
Helixi99 – 107Combined sources9
Helixi114 – 118Combined sources5
Helixi119 – 121Combined sources3
Beta strandi124 – 126Combined sources3
Beta strandi128 – 134Combined sources7
Helixi137 – 142Combined sources6
Helixi149 – 151Combined sources3
Helixi154 – 167Combined sources14
Beta strandi171 – 173Combined sources3
Beta strandi175 – 179Combined sources5
Helixi184 – 201Combined sources18
Helixi202 – 204Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1E2DX-ray1.65A1-212[»]
1E2EX-ray2.00A1-212[»]
1E2FX-ray1.60A1-212[»]
1E2GX-ray1.70A1-212[»]
1E2QX-ray1.70A1-212[»]
1E98X-ray1.90A1-212[»]
1E99X-ray1.80A1-212[»]
1E9AX-ray1.60A1-212[»]
1E9BX-ray1.70A1-212[»]
1E9CX-ray1.60A1-212[»]
1E9DX-ray1.70A1-212[»]
1E9EX-ray1.60A1-212[»]
1E9FX-ray1.90A1-144[»]
A149-212[»]
1NMXX-ray1.70A1-212[»]
1NMYX-ray1.60A4-212[»]
1NMZX-ray1.75A1-212[»]
1NN0X-ray1.60A1-212[»]
1NN1X-ray1.90A1-212[»]
1NN3X-ray1.55A1-212[»]
1NN5X-ray1.50A1-212[»]
2XX3X-ray2.00A1-212[»]
ProteinModelPortaliP23919.
SMRiP23919.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP23919.

Family & Domainsi

Sequence similaritiesi

Belongs to the thymidylate kinase family.Curated

Phylogenomic databases

eggNOGiKOG3327. Eukaryota.
COG0125. LUCA.
GeneTreeiENSGT00390000017546.
HOGENOMiHOG000229079.
HOVERGENiHBG051418.
InParanoidiP23919.
KOiK00943.
OMAiDLVMFLQ.
OrthoDBiEOG091G0M12.
PhylomeDBiP23919.
TreeFamiTF324638.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00165. Thymidylate_kinase. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR018095. Thymidylate_kin_CS.
IPR018094. Thymidylate_kinase.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00041. DTMP_kinase. 1 hit.
PROSITEiPS01331. THYMIDYLATE_KINASE. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P23919-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAARRGALIV LEGVDRAGKS TQSRKLVEAL CAAGHRAELL RFPERSTEIG
60 70 80 90 100
KLLSSYLQKK SDVEDHSVHL LFSANRWEQV PLIKEKLSQG VTLVVDRYAF
110 120 130 140 150
SGVAFTGAKE NFSLDWCKQP DVGLPKPDLV LFLQLQLADA AKRGAFGHER
160 170 180 190 200
YENGAFQERA LRCFHQLMKD TTLNWKMVDA SKSIEAVHED IRVLSEDAIR
210
TATEKPLGEL WK
Length:212
Mass (Da):23,819
Last modified:December 7, 2004 - v4
Checksum:iA52876625B3621B1
GO
Isoform 2 (identifier: P23919-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     111-134: Missing.

Note: No experimental confirmation available.
Show »
Length:188
Mass (Da):21,064
Checksum:i38FE4F99F6DDA886
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti3A → P in CAG46783 (Ref. 3) Curated1
Sequence conflicti31 – 37CAAGHRA → SRGPPP in CAA38528 (PubMed:2017365).Curated7
Sequence conflicti58Q → K in AAA21719 (PubMed:8024690).Curated1
Sequence conflicti68V → A in CAG46783 (Ref. 3) Curated1
Sequence conflicti183 – 184SI → RL in CAA38528 (PubMed:2017365).Curated2
Sequence conflicti190 – 191DI → EL (PubMed:2017365).Curated2
Sequence conflicti190 – 191DI → EL (PubMed:8024690).Curated2

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_054164111 – 134Missing in isoform 2. 1 PublicationAdd BLAST24

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X54729 mRNA. Translation: CAA38528.1.
L16991 mRNA. Translation: AAA21719.1.
CR541986 mRNA. Translation: CAG46783.1.
BC001827 mRNA. Translation: AAH01827.1.
BC171902 mRNA. Translation: AAI71902.1.
CCDSiCCDS2552.1. [P23919-1]
PIRiS26845.
RefSeqiNP_001158503.1. NM_001165031.1. [P23919-2]
NP_001307832.1. NM_001320903.1.
NP_036277.2. NM_012145.3. [P23919-1]
UniGeneiHs.471873.

Genome annotation databases

EnsembliENST00000305784; ENSP00000304802; ENSG00000168393. [P23919-1]
GeneIDi1841.
KEGGihsa:1841.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X54729 mRNA. Translation: CAA38528.1.
L16991 mRNA. Translation: AAA21719.1.
CR541986 mRNA. Translation: CAG46783.1.
BC001827 mRNA. Translation: AAH01827.1.
BC171902 mRNA. Translation: AAI71902.1.
CCDSiCCDS2552.1. [P23919-1]
PIRiS26845.
RefSeqiNP_001158503.1. NM_001165031.1. [P23919-2]
NP_001307832.1. NM_001320903.1.
NP_036277.2. NM_012145.3. [P23919-1]
UniGeneiHs.471873.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1E2DX-ray1.65A1-212[»]
1E2EX-ray2.00A1-212[»]
1E2FX-ray1.60A1-212[»]
1E2GX-ray1.70A1-212[»]
1E2QX-ray1.70A1-212[»]
1E98X-ray1.90A1-212[»]
1E99X-ray1.80A1-212[»]
1E9AX-ray1.60A1-212[»]
1E9BX-ray1.70A1-212[»]
1E9CX-ray1.60A1-212[»]
1E9DX-ray1.70A1-212[»]
1E9EX-ray1.60A1-212[»]
1E9FX-ray1.90A1-144[»]
A149-212[»]
1NMXX-ray1.70A1-212[»]
1NMYX-ray1.60A4-212[»]
1NMZX-ray1.75A1-212[»]
1NN0X-ray1.60A1-212[»]
1NN1X-ray1.90A1-212[»]
1NN3X-ray1.55A1-212[»]
1NN5X-ray1.50A1-212[»]
2XX3X-ray2.00A1-212[»]
ProteinModelPortaliP23919.
SMRiP23919.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi108174. 8 interactors.
IntActiP23919. 3 interactors.
MINTiMINT-3010142.
STRINGi9606.ENSP00000304802.

Chemistry databases

BindingDBiP23919.
ChEMBLiCHEMBL4388.

PTM databases

iPTMnetiP23919.
PhosphoSitePlusiP23919.
SwissPalmiP23919.

Polymorphism and mutation databases

BioMutaiDTYMK.
DMDMi56405325.

Proteomic databases

EPDiP23919.
PaxDbiP23919.
PeptideAtlasiP23919.
PRIDEiP23919.

Protocols and materials databases

DNASUi1841.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000305784; ENSP00000304802; ENSG00000168393. [P23919-1]
GeneIDi1841.
KEGGihsa:1841.

Organism-specific databases

CTDi1841.
DisGeNETi1841.
GeneCardsiDTYMK.
H-InvDBHIX0161852.
HGNCiHGNC:3061. DTYMK.
HPAiHPA042593.
HPA042719.
MIMi188345. gene.
neXtProtiNX_P23919.
OpenTargetsiENSG00000168393.
PharmGKBiPA150.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3327. Eukaryota.
COG0125. LUCA.
GeneTreeiENSGT00390000017546.
HOGENOMiHOG000229079.
HOVERGENiHBG051418.
InParanoidiP23919.
KOiK00943.
OMAiDLVMFLQ.
OrthoDBiEOG091G0M12.
PhylomeDBiP23919.
TreeFamiTF324638.

Enzyme and pathway databases

UniPathwayiUPA00575.
BioCyciMetaCyc:HS11626-MONOMER.
ZFISH:HS11626-MONOMER.
BRENDAi2.7.4.9. 2681.
ReactomeiR-HSA-499943. Synthesis and interconversion of nucleotide di- and triphosphates.
SABIO-RKP23919.

Miscellaneous databases

EvolutionaryTraceiP23919.
GeneWikiiDTYMK.
GenomeRNAii1841.
PROiP23919.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000168393.
CleanExiHS_DTYMK.
ExpressionAtlasiP23919. baseline and differential.
GenevisibleiP23919. HS.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00165. Thymidylate_kinase. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR018095. Thymidylate_kin_CS.
IPR018094. Thymidylate_kinase.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00041. DTMP_kinase. 1 hit.
PROSITEiPS01331. THYMIDYLATE_KINASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiKTHY_HUMAN
AccessioniPrimary (citable) accession number: P23919
Secondary accession number(s): B7ZW70, Q6FGX1, Q9BUX4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: December 7, 2004
Last modified: November 30, 2016
This is version 163 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.