P23919 (KTHY_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Thymidylate kinase
EC=2.7.4.9

Alternative name(s):
dTMP kinase

Gene names

Name:	DTYMK
Synonyms:	CDC8, TMPK, TYMK

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	212 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the conversion of dTMP to dTDP.
Catalytic activity	ATP + dTMP = ADP + dTDP.
Pathway	Pyrimidine metabolism; dTTP biosynthesis.
Sequence similarities	Belongs to the thymidylate kinase family.

Ontologies

Keywords
Biological process	Nucleotide biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Acetylation
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cell cycle Traceable author statement. Source: ProtInc cell proliferation Traceable author statement. Source: ProtInc nucleobase-containing small molecule interconversion Traceable author statement. Source: Reactome
Cellular component	cytosol Traceable author statement. Source: Reactome
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW nucleoside phosphate kinase activity Inferred from experiment. Source: Reactome thymidylate kinase activity Inferred from direct assay. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed

Chain

2 – 212

211

Thymidylate kinase

PRO_0000155210

Regions

Nucleotide binding

13 – 20

8

ATP Probable

Amino acid modifications

Modified residue

2

1

N-acetylalanine Ref.5

Modified residue

169

1

N6-acetyllysine Ref.6

Experimental info

Sequence conflict

3

1

A → P in CAG46783. Ref.3

Sequence conflict

31 – 37

7

CAAGHRA → SRGPPP in CAA38528. Ref.1

Sequence conflict

58

1

Q → K in AAA21719. Ref.2

Sequence conflict

68

1

V → A in CAG46783. Ref.3

Sequence conflict

183 – 184

2

SI → RL in CAA38528. Ref.1

Sequence conflict

190 – 191

2

DI → EL Ref.1

Sequence conflict

190 – 191

2

DI → EL Ref.2

Secondary structure

1

.

.

.

.

.

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.

.

.

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212

Helix Strand Turn

Details...

Beta strand

8 – 14

7

Helix

19 – 32

14

Beta strand

37 – 43

7

Helix

48 – 57

10

Helix

65 – 77

13

Helix

80 – 88

9

Beta strand

92 – 97

6

Helix

99 – 107

9

Helix

114 – 118

5

Helix

119 – 121

3

Beta strand

128 – 134

7

Helix

137 – 141

5

Helix

154 – 167

14

Beta strand

175 – 179

5

Helix

184 – 202

19

Sequences

	Sequence		Length	Mass (Da)
	P23919 [UniParc]. Last modified December 7, 2004. Version 4. Checksum: A52876625B3621B1	FASTA	212	23,819
	10 20 30 40 50 60 MAARRGALIV LEGVDRAGKS TQSRKLVEAL CAAGHRAELL RFPERSTEIG KLLSSYLQKK 70 80 90 100 110 120 SDVEDHSVHL LFSANRWEQV PLIKEKLSQG VTLVVDRYAF SGVAFTGAKE NFSLDWCKQP 130 140 150 160 170 180 DVGLPKPDLV LFLQLQLADA AKRGAFGHER YENGAFQERA LRCFHQLMKD TTLNWKMVDA 190 200 210 SKSIEAVHED IRVLSEDAIR TATEKPLGEL WK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and expression of the human deoxythymidylate kinase gene in yeast." Su J.Y., Sclafani R.A. Nucleic Acids Res. 19:823-827(1991) [PubMed: 2017365] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Human dTMP kinase: gene expression and enzymatic activity coinciding with cell cycle progression and cell growth." Huang S.H., Tang A., Drisco B., Zhang S.Q., Seeger R., Li C., Jong A. DNA Cell Biol. 13:461-471(1994) [PubMed: 8024690] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S., Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W., Korn B., Zuo D., Hu Y., LaBaer J. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Muscle.
[5]	"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach." Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S. Anal. Chem. 81:4493-4501(2009) [PubMed: 19413330] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, MASS SPECTROMETRY. Tissue: Embryonic kidney.
[6]	"Lysine acetylation targets protein complexes and co-regulates major cellular functions." Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T., Olsen J.V., Mann M. Science 325:834-840(2009) [PubMed: 19608861] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-169, MASS SPECTROMETRY.
[7]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed: 21269460] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[8]	"Structures of human thymidylate kinase in complex with prodrugs: implications for the structure-based design of novel compounds." Ostermann N., Segura-Pena D., Meier C., Veit T., Monnerjahn C., Konrad M., Lavie A. Biochemistry 42:2568-2577(2003) [PubMed: 12614151] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.65 ANGSTROMS).
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X54729 mRNA. Translation: CAA38528.1.
L16991 mRNA. Translation: AAA21719.1.
CR541986 mRNA. Translation: CAG46783.1.
BC001827 mRNA. Translation: AAH01827.1.

IPI

IPI00013862.

PIR

S26845.

RefSeq

NP_036277.2. NM_012145.3.

UniGene

Hs.471873.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1E2D	X-ray	1.65	A	1-212	[»]
1E2E	X-ray	2.00	A	1-212	[»]
1E2F	X-ray	1.60	A	1-212	[»]
1E2G	X-ray	1.70	A	1-212	[»]
1E2Q	X-ray	1.70	A	1-212	[»]
1E98	X-ray	1.90	A	1-212	[»]
1E99	X-ray	1.80	A	1-212	[»]
1E9A	X-ray	1.60	A	1-212	[»]
1E9B	X-ray	1.70	A	1-212	[»]
1E9C	X-ray	1.60	A	1-212	[»]
1E9D	X-ray	1.70	A	1-212	[»]
1E9E	X-ray	1.60	A	1-212	[»]
1E9F	X-ray	1.90	A	1-212	[»]
1NMX	X-ray	1.70	A	1-212	[»]
1NMY	X-ray	1.60	A	4-212	[»]
1NMZ	X-ray	1.75	A	1-212	[»]
1NN0	X-ray	1.60	A	1-212	[»]
1NN1	X-ray	1.90	A	1-212	[»]
1NN3	X-ray	1.55	A	1-212	[»]
1NN5	X-ray	1.50	A	1-212	[»]

ProteinModelPortal

P23919.

SMR

P23919. Positions 4-212.

ModBase

Search...

Protein-protein interaction databases

IntAct

P23919. 1 interaction.

STRING

P23919.

PTM databases

PhosphoSite

P23919.

Polymorphism databases

DMDM

56405325.

Proteomic databases

PRIDE

P23919.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000305784; ENSP00000304802; ENSG00000168393.

GeneID

1841.

KEGG

hsa:1841.

UCSC

uc002wbz.1. human.

Organism-specific databases

CTD

1841.

GeneCards

GC02M242634.

HGNC

HGNC:3061. DTYMK.

HPA

HPA042593.
HPA042719.

MIM

188345. gene.

neXtProt

NX_P23919.

PharmGKB

PA150.

GenAtlas

Search...

Phylogenomic databases

HOGENOM

HBG626009.

HOVERGEN

HBG051418.

InParanoid

P23919.

OMA

YSGVAYS.

OrthoDB

EOG4KKZ46.

PhylomeDB

P23919.

Enzyme and pathway databases

Reactome

REACT_111217. Metabolism.

Gene expression databases

ArrayExpress

P23919.

Bgee

P23919.

CleanEx

HS_DTYMK.

Genevestigator

P23919.

GermOnline

ENSG00000168393. Homo sapiens.

Family and domain databases

InterPro

IPR018095. Thymidylate_kin_CS.
IPR018094. Thymidylate_kinase.
[Graphical view]

KO

K00943.

PANTHER

PTHR10344. Thymidylate_kin. 1 hit.

Pfam

PF02223. Thymidylate_kin. 1 hit.
[Graphical view]

TIGRFAMs

TIGR00041. DTMP_kinase. 1 hit.

PROSITE

PS01331. THYMIDYLATE_KINASE. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

7537.

SOURCE

Search...

Entry information

Entry name

KTHY_HUMAN

Accession

Primary (citable) accession number: P23919
Secondary accession number(s): Q6FGX1, Q9BUX4

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 1992
Last sequence update:	December 7, 2004
Last modified:	January 25, 2012
This is version 114 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.