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Protein

Fructokinase

Gene

mak

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the phosphorylation of fructose to fructose-6-P. Has also low level glucokinase activity in vitro. Is not able to phosphorylate D-ribose, D-mannitol, D-sorbitol, inositol, and L-threonine.2 Publications

Catalytic activityi

ATP + D-fructose = ADP + D-fructose 6-phosphate.

Kineticsi

Catalytic efficiency with D-fructose as substrate is 55-fold higher than that with D-glucose.

  1. KM=1.3 mM for D-fructose (at 25 degrees Celsius and pH 7.6)1 Publication
  2. KM=1.8 mM for ATP (at 25 degrees Celsius and pH 7.6)1 Publication
  3. KM=59 mM for glucose (at 25 degrees Celsius and pH 7.6)1 Publication

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi4 – 118ATPSequence analysis
    Nucleotide bindingi133 – 1408ATPSequence analysis

    GO - Molecular functioni

    • ATP binding Source: UniProtKB-KW
    • fructokinase activity Source: UniProtKB-EC
    • hexokinase activity Source: EcoCyc

    GO - Biological processi

    • hexose metabolic process Source: EcoCyc
    Complete GO annotation...

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Carbohydrate metabolism

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciEcoCyc:EG11288-MONOMER.
    ECOL316407:JW0385-MONOMER.
    MetaCyc:EG11288-MONOMER.
    SABIO-RKP23917.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Fructokinase (EC:2.7.1.4)
    Alternative name(s):
    D-fructose kinase
    Manno(fructo)kinase
    Gene namesi
    Name:mak
    Synonyms:yajF
    Ordered Locus Names:b0394, JW0385
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    Proteomesi
    • UP000000318 Componenti: Chromosome
    • UP000000625 Componenti: Chromosome

    Organism-specific databases

    EcoGeneiEG11288. mak.

    Subcellular locationi

    GO - Cellular componenti

    • cytosol Source: EcoCyc
    Complete GO annotation...

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 302302FructokinasePRO_0000095689Add
    BLAST

    Proteomic databases

    EPDiP23917.
    PaxDbiP23917.
    PRIDEiP23917.

    Interactioni

    Protein-protein interaction databases

    BioGridi4259818. 3 interactions.
    DIPiDIP-11288N.
    IntActiP23917. 2 interactions.
    STRINGi511145.b0394.

    Structurei

    3D structure databases

    ProteinModelPortaliP23917.
    SMRiP23917. Positions 1-298.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ROK (NagC/XylR) family.Curated

    Phylogenomic databases

    eggNOGiENOG4105DKQ. Bacteria.
    COG1940. LUCA.
    HOGENOMiHOG000150087.
    InParanoidiP23917.
    KOiK00847.
    OMAiLEAWYEA.
    OrthoDBiEOG61P6P0.
    PhylomeDBiP23917.

    Family and domain databases

    InterProiIPR000600. ROK.
    [Graphical view]
    PfamiPF00480. ROK. 1 hit.
    [Graphical view]
    PROSITEiPS01125. ROK. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P23917-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MRIGIDLGGT KTEVIALGDA GEQLYRHRLP TPRDDYRQTI ETIATLVDMA
    60 70 80 90 100
    EQATGQRGTV GMGIPGSISP YTGVVKNANS TWLNGQPFDK DLSARLQREV
    110 120 130 140 150
    RLANDANCLA VSEAVDGAAA GAQTVFAVII GTGCGAGVAF NGRAHIGGNG
    160 170 180 190 200
    TAGEWGHNPL PWMDEDELRY REEVPCYCGK QGCIETFISG TGFAMDYRRL
    210 220 230 240 250
    SGHALKGSEI IRLVEESDPV AELALRRYEL RLAKSLAHVV NILDPDVIVL
    260 270 280 290 300
    GGGMSNVDRL YQTVGQLIKQ FVFGGECETP VRKAKHGDSS GVRGAAWLWP

    QE
    Length:302
    Mass (Da):32,500
    Last modified:June 1, 1994 - v2
    Checksum:i9791F9C29C91049C
    GO

    Sequence cautioni

    The sequence AAB18118.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    X76979 Genomic DNA. Translation: CAA54284.1.
    U73857 Genomic DNA. Translation: AAB18118.1. Different initiation.
    U00096 Genomic DNA. Translation: AAC73497.2.
    AP009048 Genomic DNA. Translation: BAE76175.1.
    M64787 Genomic DNA. Translation: AAA23475.1.
    PIRiB64768.
    RefSeqiNP_414928.2. NC_000913.3.
    WP_001219309.1. NZ_LN832404.1.

    Genome annotation databases

    EnsemblBacteriaiAAC73497; AAC73497; b0394.
    BAE76175; BAE76175; BAE76175.
    GeneIDi949086.
    KEGGiecj:JW0385.
    eco:b0394.
    PATRICi32115931. VBIEscCol129921_0407.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    X76979 Genomic DNA. Translation: CAA54284.1.
    U73857 Genomic DNA. Translation: AAB18118.1. Different initiation.
    U00096 Genomic DNA. Translation: AAC73497.2.
    AP009048 Genomic DNA. Translation: BAE76175.1.
    M64787 Genomic DNA. Translation: AAA23475.1.
    PIRiB64768.
    RefSeqiNP_414928.2. NC_000913.3.
    WP_001219309.1. NZ_LN832404.1.

    3D structure databases

    ProteinModelPortaliP23917.
    SMRiP23917. Positions 1-298.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi4259818. 3 interactions.
    DIPiDIP-11288N.
    IntActiP23917. 2 interactions.
    STRINGi511145.b0394.

    Proteomic databases

    EPDiP23917.
    PaxDbiP23917.
    PRIDEiP23917.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiAAC73497; AAC73497; b0394.
    BAE76175; BAE76175; BAE76175.
    GeneIDi949086.
    KEGGiecj:JW0385.
    eco:b0394.
    PATRICi32115931. VBIEscCol129921_0407.

    Organism-specific databases

    EchoBASEiEB1265.
    EcoGeneiEG11288. mak.

    Phylogenomic databases

    eggNOGiENOG4105DKQ. Bacteria.
    COG1940. LUCA.
    HOGENOMiHOG000150087.
    InParanoidiP23917.
    KOiK00847.
    OMAiLEAWYEA.
    OrthoDBiEOG61P6P0.
    PhylomeDBiP23917.

    Enzyme and pathway databases

    BioCyciEcoCyc:EG11288-MONOMER.
    ECOL316407:JW0385-MONOMER.
    MetaCyc:EG11288-MONOMER.
    SABIO-RKP23917.

    Miscellaneous databases

    PROiP23917.

    Family and domain databases

    InterProiIPR000600. ROK.
    [Graphical view]
    PfamiPF00480. ROK. 1 hit.
    [Graphical view]
    PROSITEiPS01125. ROK. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Publicationsi

    « Hide 'large scale' publications
    1. "Recombination-dependent growth in exonuclease-depleted recBC sbcBC strains of Escherichia coli K-12."
      Ryder L., Sharples G.J., Lloyd R.G.
      Genetics 143:1101-1114(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: K12.
    2. "Sequence of minutes 4-25 of Escherichia coli."
      Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.
      Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    5. "Mapping, sequence, and apparent lack of function of araJ, a gene of the Escherichia coli arabinose regulon."
      Reeder T.C., Schleif R.F.
      J. Bacteriol. 173:7765-7771(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 43-302.
    6. "Genetic control of manno(fructo)kinase activity in Escherichia coli."
      Sproul A.A., Lambourne L.T., Jims J.-J.D., Kornberg H.L.
      Proc. Natl. Acad. Sci. U.S.A. 98:15257-15259(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: ROLE IN FRUCTOSE METABOLISM.
    7. "Identifying latent enzyme activities: substrate ambiguity within modern bacterial sugar kinases."
      Miller B.G., Raines R.T.
      Biochemistry 43:6387-6392(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBSTRATE SPECIFICITY, KINETIC PARAMETERS.
      Strain: K12 / MC4100 / ATCC 35695 / DSM 6574.

    Entry informationi

    Entry nameiMAK_ECOLI
    AccessioniPrimary (citable) accession number: P23917
    Secondary accession number(s): P71316, P75705, Q2MC31
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 1, 1992
    Last sequence update: June 1, 1994
    Last modified: July 6, 2016
    This is version 111 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.