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Protein

Fructokinase

Gene

mak

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the phosphorylation of fructose to fructose-6-P. Has also low level glucokinase activity in vitro. Is not able to phosphorylate D-ribose, D-mannitol, D-sorbitol, inositol, and L-threonine.2 Publications

Catalytic activityi

ATP + D-fructose = ADP + D-fructose 6-phosphate.

Kineticsi

Catalytic efficiency with D-fructose as substrate is 55-fold higher than that with D-glucose.
  1. KM=1.3 mM for D-fructose (at 25 degrees Celsius and pH 7.6)1 Publication
  2. KM=1.8 mM for ATP (at 25 degrees Celsius and pH 7.6)1 Publication
  3. KM=59 mM for glucose (at 25 degrees Celsius and pH 7.6)1 Publication

    Regions

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Nucleotide bindingi4 – 11ATPSequence analysis8
    Nucleotide bindingi133 – 140ATPSequence analysis8

    GO - Molecular functioni

    • ATP binding Source: UniProtKB-KW
    • fructokinase activity Source: UniProtKB-EC
    • hexokinase activity Source: EcoCyc

    GO - Biological processi

    • hexose metabolic process Source: EcoCyc

    Keywordsi

    Molecular functionKinase, Transferase
    Biological processCarbohydrate metabolism
    LigandATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciEcoCyc:EG11288-MONOMER
    MetaCyc:EG11288-MONOMER
    SABIO-RKP23917

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Fructokinase (EC:2.7.1.4)
    Alternative name(s):
    D-fructose kinase
    Manno(fructo)kinase
    Gene namesi
    Name:mak
    Synonyms:yajF
    Ordered Locus Names:b0394, JW0385
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
    Proteomesi
    • UP000000318 Componenti: Chromosome
    • UP000000625 Componenti: Chromosome

    Organism-specific databases

    EcoGeneiEG11288 mak

    Subcellular locationi

    GO - Cellular componenti

    • cytosol Source: EcoCyc

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00000956891 – 302FructokinaseAdd BLAST302

    Proteomic databases

    PaxDbiP23917
    PRIDEiP23917

    Interactioni

    Protein-protein interaction databases

    BioGridi4259818, 15 interactors
    DIPiDIP-11288N
    IntActiP23917, 30 interactors
    STRINGi316385.ECDH10B_0351

    Structurei

    3D structure databases

    ProteinModelPortaliP23917
    SMRiP23917
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ROK (NagC/XylR) family.Curated

    Phylogenomic databases

    eggNOGiENOG4105DKQ Bacteria
    COG1940 LUCA
    HOGENOMiHOG000150087
    InParanoidiP23917
    KOiK00847
    OMAiGAPCNCG
    PhylomeDBiP23917

    Family and domain databases

    InterProiView protein in InterPro
    IPR000600 ROK
    PANTHERiPTHR18964 PTHR18964, 1 hit
    PfamiView protein in Pfam
    PF00480 ROK, 1 hit
    PROSITEiView protein in PROSITE
    PS01125 ROK, 1 hit

    Sequencei

    Sequence statusi: Complete.

    P23917-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MRIGIDLGGT KTEVIALGDA GEQLYRHRLP TPRDDYRQTI ETIATLVDMA
    60 70 80 90 100
    EQATGQRGTV GMGIPGSISP YTGVVKNANS TWLNGQPFDK DLSARLQREV
    110 120 130 140 150
    RLANDANCLA VSEAVDGAAA GAQTVFAVII GTGCGAGVAF NGRAHIGGNG
    160 170 180 190 200
    TAGEWGHNPL PWMDEDELRY REEVPCYCGK QGCIETFISG TGFAMDYRRL
    210 220 230 240 250
    SGHALKGSEI IRLVEESDPV AELALRRYEL RLAKSLAHVV NILDPDVIVL
    260 270 280 290 300
    GGGMSNVDRL YQTVGQLIKQ FVFGGECETP VRKAKHGDSS GVRGAAWLWP

    QE
    Length:302
    Mass (Da):32,500
    Last modified:June 1, 1994 - v2
    Checksum:i9791F9C29C91049C
    GO

    Sequence cautioni

    The sequence AAB18118 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    X76979 Genomic DNA Translation: CAA54284.1
    U73857 Genomic DNA Translation: AAB18118.1 Different initiation.
    U00096 Genomic DNA Translation: AAC73497.2
    AP009048 Genomic DNA Translation: BAE76175.1
    M64787 Genomic DNA Translation: AAA23475.1
    PIRiB64768
    RefSeqiNP_414928.2, NC_000913.3
    WP_001219309.1, NZ_LN832404.1

    Genome annotation databases

    EnsemblBacteriaiAAC73497; AAC73497; b0394
    BAE76175; BAE76175; BAE76175
    GeneIDi949086
    KEGGiecj:JW0385
    eco:b0394
    PATRICifig|1411691.4.peg.1884

    Similar proteinsi

    Entry informationi

    Entry nameiMAK_ECOLI
    AccessioniPrimary (citable) accession number: P23917
    Secondary accession number(s): P71316, P75705, Q2MC31
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 1, 1992
    Last sequence update: June 1, 1994
    Last modified: March 28, 2018
    This is version 121 of the entry and version 2 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

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