P23917 (MAK_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Fructokinase
EC=2.7.1.4

Alternative name(s):
D-fructose kinase
Manno(fructo)kinase

Gene names

Name:	mak
Synonyms:	yajF
Ordered Locus Names:	b0394, JW0385

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	302 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the phosphorylation of fructose to fructose-6-P. Has also low level glucokinase activity in vitro. Is not able to phosphorylate D-ribose, D-mannitol, D-sorbitol, inositol, and L-threonine. Ref.6 Ref.7
Catalytic activity	ATP + D-fructose = ADP + D-fructose 6-phosphate.
Sequence similarities	Belongs to the ROK (nagC/xylR) family.
Biophysicochemical properties	Kinetic parameters: Catalytic efficiency with D-fructose as substrate is 55-fold higher than that with D-glucose. KM=1.3 mM for D-fructose (at 25 degrees Celsius and pH 7.6) Ref.7 KM=1.8 mM for ATP (at 25 degrees Celsius and pH 7.6) KM=59 mM for glucose (at 25 degrees Celsius and pH 7.6)
Sequence caution	The sequence AAB18118.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
Biological process	Carbohydrate metabolism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW fructokinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 302	302	Fructokinase		PRO_0000095689
Regions
	Nucleotide binding	4 – 11	8	ATP Potential
	Nucleotide binding	133 – 140	8	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	P23917 [UniParc]. Last modified June 1, 1994. Version 2. Checksum: 9791F9C29C91049C	FASTA	302	32,500
	10 20 30 40 50 60 MRIGIDLGGT KTEVIALGDA GEQLYRHRLP TPRDDYRQTI ETIATLVDMA EQATGQRGTV 70 80 90 100 110 120 GMGIPGSISP YTGVVKNANS TWLNGQPFDK DLSARLQREV RLANDANCLA VSEAVDGAAA 130 140 150 160 170 180 GAQTVFAVII GTGCGAGVAF NGRAHIGGNG TAGEWGHNPL PWMDEDELRY REEVPCYCGK 190 200 210 220 230 240 QGCIETFISG TGFAMDYRRL SGHALKGSEI IRLVEESDPV AELALRRYEL RLAKSLAHVV 250 260 270 280 290 300 NILDPDVIVL GGGMSNVDRL YQTVGQLIKQ FVFGGECETP VRKAKHGDSS GVRGAAWLWP QE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Recombination-dependent growth in exonuclease-depleted recBC sbcBC strains of Escherichia coli K-12." Ryder L., Sharples G.J., Lloyd R.G. Genetics 143:1101-1114(1996) [PubMed: 8807285] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"Sequence of minutes 4-25 of Escherichia coli." Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W. Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Mapping, sequence, and apparent lack of function of araJ, a gene of the Escherichia coli arabinose regulon." Reeder T.C., Schleif R.F. J. Bacteriol. 173:7765-7771(1991) [PubMed: 1744033] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 43-302.
[6]	"Genetic control of manno(fructo)kinase activity in Escherichia coli." Sproul A.A., Lambourne L.T., Jims J.-J.D., Kornberg H.L. Proc. Natl. Acad. Sci. U.S.A. 98:15257-15259(2001) [PubMed: 11742072] [Abstract] Cited for: ROLE IN FRUCTOSE METABOLISM.
[7]	"Identifying latent enzyme activities: substrate ambiguity within modern bacterial sugar kinases." Miller B.G., Raines R.T. Biochemistry 43:6387-6392(2004) [PubMed: 15157072] [Abstract] Cited for: FUNCTION, SUBSTRATE SPECIFICITY, KINETIC PARAMETERS. Strain: K12 / MC4100 / ATCC 35695 / DSM 6574.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X76979 Genomic DNA. Translation: CAA54284.1. U73857 Genomic DNA. Translation: AAB18118.1. Different initiation. U00096 Genomic DNA. Translation: AAC73497.2. AP009048 Genomic DNA. Translation: BAE76175.1. M64787 Genomic DNA. Translation: AAA23475.1.
PIR	B64768.
RefSeq	NP_414928.2. NC_000913.2.
3D structure databases
ProteinModelPortal	P23917.
SMR	P23917. Positions 1-302.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-11288N.
IntAct	P23917. 2 interactions.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000002837; EBESCP00000002837; EBESCG00000002314. EBESCT00000017778; EBESCP00000017069; EBESCG00000016834.
GeneID	949086.
GenomeReviews	Gene locus JW0385 in contig AP009048_GR. Gene locus b0394 in contig U00096_GR.
KEGG	ecj:JW0385. eco:b0394.
PATRIC	32115931. VBIEscCol129921_0407.
Organism-specific databases
EchoBASE	EB1265.
EcoGene	EG11288. mak.
Phylogenomic databases
eggNOG	COG1940.
GeneTree	EBGT00050000009665.
HOGENOM	HBG730260.
OMA	ECETPIR.
PhylomeDB	P23917.
ProtClustDB	PRK09557.
Enzyme and pathway databases
BioCyc	EcoCyc:EG11288-MONOMER. MetaCyc:EG11288-MONOMER.
Gene expression databases
Genevestigator	P23917.
Family and domain databases
InterPro	IPR000600. ROK. [Graphical view]
KO	K00847.
Pfam	PF00480. ROK. 1 hit. [Graphical view]
PROSITE	PS01125. ROK. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MAK_ECOLI

Accession

Primary (citable) accession number: P23917
Secondary accession number(s): P71316, P75705, Q2MC31

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 1992
Last sequence update:	June 1, 1994
Last modified:	January 25, 2012
This is version 82 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program