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Protein

DNA mismatch repair protein MutS

Gene

mutS

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

This protein is involved in the repair of mismatches in DNA. It is possible that it carries out the mismatch recognition step. This protein has a weak ATPase activity.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi614 – 621ATPSequence analysis8

GO - Molecular functioni

  • adenine/cytosine mispair binding Source: EcoliWiki
  • ADP binding Source: EcoliWiki
  • ATPase activity Source: EcoliWiki
  • ATP binding Source: EcoliWiki
  • damaged DNA binding Source: UniProtKB-HAMAP
  • DNA binding, bending Source: EcoliWiki
  • mismatched DNA binding Source: EcoliWiki

GO - Biological processi

  • cellular response to DNA damage stimulus Source: EcoliWiki
  • mismatch repair Source: EcoliWiki
  • regulation of DNA recombination Source: EcoliWiki
Complete GO annotation...

Keywords - Biological processi

DNA damage, DNA repair

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG10625-MONOMER.
ECOL316407:JW2703-MONOMER.
MetaCyc:EG10625-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA mismatch repair protein MutS
Gene namesi
Name:mutS
Synonyms:fdv
Ordered Locus Names:b2733, JW2703
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10625. mutS.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc
  • mismatch repair complex Source: EcoliWiki
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001150941 – 853DNA mismatch repair protein MutSAdd BLAST853

Proteomic databases

PaxDbiP23909.
PRIDEiP23909.

2D gel databases

SWISS-2DPAGEP23909.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
itself3EBI-554920,EBI-554920

Protein-protein interaction databases

BioGridi4261421. 115 interactors.
DIPiDIP-10287N.
IntActiP23909. 10 interactors.
MINTiMINT-1264156.
STRINGi511145.b2733.

Structurei

Secondary structure

1853
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi7 – 9Combined sources3
Helixi12 – 23Combined sources12
Beta strandi27 – 33Combined sources7
Beta strandi36 – 40Combined sources5
Helixi41 – 51Combined sources11
Beta strandi56 – 58Combined sources3
Beta strandi61 – 63Combined sources3
Beta strandi66 – 72Combined sources7
Helixi73 – 75Combined sources3
Helixi76 – 85Combined sources10
Beta strandi90 – 95Combined sources6
Helixi99 – 101Combined sources3
Beta strandi103 – 105Combined sources3
Beta strandi108 – 114Combined sources7
Turni116 – 118Combined sources3
Helixi122 – 124Combined sources3
Beta strandi133 – 138Combined sources6
Beta strandi143 – 148Combined sources6
Turni150 – 152Combined sources3
Beta strandi155 – 158Combined sources4
Helixi163 – 173Combined sources11
Beta strandi176 – 181Combined sources6
Helixi187 – 189Combined sources3
Turni190 – 192Combined sources3
Beta strandi194 – 199Combined sources6
Helixi201 – 204Combined sources4
Helixi206 – 217Combined sources12
Beta strandi220 – 222Combined sources3
Helixi223 – 225Combined sources3
Helixi231 – 248Combined sources18
Beta strandi258 – 260Combined sources3
Helixi263 – 265Combined sources3
Helixi271 – 276Combined sources6
Beta strandi279 – 281Combined sources3
Beta strandi287 – 290Combined sources4
Helixi291 – 295Combined sources5
Helixi301 – 312Combined sources12
Helixi318 – 330Combined sources13
Helixi332 – 334Combined sources3
Helixi335 – 343Combined sources9
Helixi348 – 356Combined sources9
Helixi362 – 372Combined sources11
Helixi375 – 383Combined sources9
Helixi388 – 397Combined sources10
Helixi401 – 410Combined sources10
Beta strandi419 – 422Combined sources4
Helixi431 – 458Combined sources28
Beta strandi464 – 468Combined sources5
Turni469 – 471Combined sources3
Beta strandi472 – 478Combined sources7
Helixi479 – 482Combined sources4
Beta strandi490 – 494Combined sources5
Beta strandi496 – 502Combined sources7
Helixi504 – 535Combined sources32
Helixi536 – 538Combined sources3
Helixi539 – 564Combined sources26
Beta strandi570 – 572Combined sources3
Beta strandi574 – 576Combined sources3
Beta strandi578 – 583Combined sources6
Helixi588 – 591Combined sources4
Beta strandi592 – 594Combined sources3
Beta strandi599 – 603Combined sources5
Beta strandi605 – 607Combined sources3
Beta strandi609 – 613Combined sources5
Helixi620 – 634Combined sources15
Turni635 – 637Combined sources3
Beta strandi640 – 647Combined sources8
Beta strandi652 – 657Combined sources6
Helixi671 – 683Combined sources13
Beta strandi688 – 694Combined sources7
Beta strandi699 – 702Combined sources4
Helixi703 – 717Combined sources15
Beta strandi722 – 726Combined sources5
Helixi730 – 734Combined sources5
Helixi735 – 738Combined sources4
Beta strandi742 – 752Combined sources11
Beta strandi755 – 765Combined sources11
Helixi772 – 778Combined sources7
Helixi783 – 797Combined sources15
Helixi826 – 831Combined sources6
Turni834 – 836Combined sources3
Helixi839 – 852Combined sources14

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1E3MX-ray2.20A/B1-800[»]
1NG9X-ray2.60A/B1-800[»]
1OH5X-ray2.90A/B1-800[»]
1OH6X-ray2.40A/B1-800[»]
1OH7X-ray2.50A/B1-800[»]
1OH8X-ray2.90A/B1-800[»]
1W7AX-ray2.27A/B1-800[»]
1WB9X-ray2.10A/B1-800[»]
1WBBX-ray2.50A/B1-800[»]
1WBDX-ray2.40A/B1-800[»]
2OK2X-ray2.00A/B820-853[»]
2WTUX-ray3.40A/B1-800[»]
3K0SX-ray2.20A/B2-800[»]
3ZLJX-ray3.10A/B1-800[»]
C/D801-853[»]
5AKBX-ray4.71A/B/E1-800[»]
5AKCX-ray6.60A/B/E/F/I/J1-800[»]
5AKDX-ray7.60A/B/E/F/I/J1-800[»]
ProteinModelPortaliP23909.
SMRiP23909.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP23909.

Family & Domainsi

Sequence similaritiesi

Belongs to the DNA mismatch repair MutS family.Curated

Phylogenomic databases

eggNOGiENOG4105D86. Bacteria.
COG0249. LUCA.
HOGENOMiHOG000221407.
InParanoidiP23909.
KOiK03555.
OMAiEMTEMAN.
PhylomeDBiP23909.

Family and domain databases

Gene3Di3.30.420.110. 1 hit.
3.40.1170.10. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_00096. MutS. 1 hit.
InterProiIPR005748. DNA_mismatch_repair_MutS-1.
IPR007695. DNA_mismatch_repair_MutS-lik_N.
IPR000432. DNA_mismatch_repair_MutS_C.
IPR007861. DNA_mismatch_repair_MutS_clamp.
IPR007696. DNA_mismatch_repair_MutS_core.
IPR016151. DNA_mismatch_repair_MutS_N.
IPR007860. DNA_mmatch_repair_MutS_con_dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF01624. MutS_I. 1 hit.
PF05188. MutS_II. 1 hit.
PF05192. MutS_III. 1 hit.
PF05190. MutS_IV. 1 hit.
PF00488. MutS_V. 1 hit.
[Graphical view]
SMARTiSM00534. MUTSac. 1 hit.
SM00533. MUTSd. 1 hit.
[Graphical view]
SUPFAMiSSF48334. SSF48334. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF53150. SSF53150. 1 hit.
SSF55271. SSF55271. 1 hit.
TIGRFAMsiTIGR01070. mutS1. 1 hit.
PROSITEiPS00486. DNA_MISMATCH_REPAIR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P23909-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSAIENFDAH TPMMQQYLRL KAQHPEILLF YRMGDFYELF YDDAKRASQL
60 70 80 90 100
LDISLTKRGA SAGEPIPMAG IPYHAVENYL AKLVNQGESV AICEQIGDPA
110 120 130 140 150
TSKGPVERKV VRIVTPGTIS DEALLQERQD NLLAAIWQDS KGFGYATLDI
160 170 180 190 200
SSGRFRLSEP ADRETMAAEL QRTNPAELLY AEDFAEMSLI EGRRGLRRRP
210 220 230 240 250
LWEFEIDTAR QQLNLQFGTR DLVGFGVENA PRGLCAAGCL LQYAKDTQRT
260 270 280 290 300
TLPHIRSITM EREQDSIIMD AATRRNLEIT QNLAGGAENT LASVLDCTVT
310 320 330 340 350
PMGSRMLKRW LHMPVRDTRV LLERQQTIGA LQDFTAGLQP VLRQVGDLER
360 370 380 390 400
ILARLALRTA RPRDLARMRH AFQQLPELRA QLETVDSAPV QALREKMGEF
410 420 430 440 450
AELRDLLERA IIDTPPVLVR DGGVIASGYN EELDEWRALA DGATDYLERL
460 470 480 490 500
EVRERERTGL DTLKVGFNAV HGYYIQISRG QSHLAPINYM RRQTLKNAER
510 520 530 540 550
YIIPELKEYE DKVLTSKGKA LALEKQLYEE LFDLLLPHLE ALQQSASALA
560 570 580 590 600
ELDVLVNLAE RAYTLNYTCP TFIDKPGIRI TEGRHPVVEQ VLNEPFIANP
610 620 630 640 650
LNLSPQRRML IITGPNMGGK STYMRQTALI ALMAYIGSYV PAQKVEIGPI
660 670 680 690 700
DRIFTRVGAA DDLASGRSTF MVEMTETANI LHNATEYSLV LMDEIGRGTS
710 720 730 740 750
TYDGLSLAWA CAENLANKIK ALTLFATHYF ELTQLPEKME GVANVHLDAL
760 770 780 790 800
EHGDTIAFMH SVQDGAASKS YGLAVAALAG VPKEVIKRAR QKLRELESIS
810 820 830 840 850
PNAAATQVDG TQMSLLSVPE ETSPAVEALE NLDPDSLTPR QALEWIYRLK

SLV
Length:853
Mass (Da):95,247
Last modified:March 1, 1992 - v1
Checksum:i5A1F295362671D55
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M64730 Genomic DNA. Translation: AAA24188.1.
U29579 Genomic DNA. Translation: AAA69243.1.
U00096 Genomic DNA. Translation: AAC75775.1.
AP009048 Genomic DNA. Translation: BAE76810.1.
AF004287 Genomic DNA. Translation: AAD01197.1.
AF001987 Genomic DNA. Translation: AAD00921.1.
AF001988 Genomic DNA. Translation: AAD00922.1.
AF001989 Genomic DNA. Translation: AAD00923.1.
AF001990 Genomic DNA. Translation: AAD00924.1.
AF001991 Genomic DNA. Translation: AAD00925.1.
AF001992 Genomic DNA. Translation: AAD00926.1.
AF001993 Genomic DNA. Translation: AAD00927.1.
AF001994 Genomic DNA. Translation: AAD00928.1.
AF001995 Genomic DNA. Translation: AAD00929.1.
AF001996 Genomic DNA. Translation: AAD00930.1.
AF001997 Genomic DNA. Translation: AAD00931.1.
AF001998 Genomic DNA. Translation: AAD00932.1.
AF001999 Genomic DNA. Translation: AAD00933.1.
AF002000 Genomic DNA. Translation: AAD00934.1.
AF002001 Genomic DNA. Translation: AAD00935.1.
AF002002 Genomic DNA. Translation: AAD00936.1.
AF002003 Genomic DNA. Translation: AAD00937.1.
AF002004 Genomic DNA. Translation: AAD00938.1.
AF002005 Genomic DNA. Translation: AAD00939.1.
AF002006 Genomic DNA. Translation: AAD00940.1.
AF002007 Genomic DNA. Translation: AAD00941.1.
AF002008 Genomic DNA. Translation: AAD00942.1.
AF002009 Genomic DNA. Translation: AAD00943.1.
AF002010 Genomic DNA. Translation: AAD00944.1.
PIRiI54964.
RefSeqiNP_417213.1. NC_000913.3.
WP_001272928.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75775; AAC75775; b2733.
BAE76810; BAE76810; BAE76810.
GeneIDi947206.
KEGGiecj:JW2703.
eco:b2733.
PATRICi32120868. VBIEscCol129921_2826.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M64730 Genomic DNA. Translation: AAA24188.1.
U29579 Genomic DNA. Translation: AAA69243.1.
U00096 Genomic DNA. Translation: AAC75775.1.
AP009048 Genomic DNA. Translation: BAE76810.1.
AF004287 Genomic DNA. Translation: AAD01197.1.
AF001987 Genomic DNA. Translation: AAD00921.1.
AF001988 Genomic DNA. Translation: AAD00922.1.
AF001989 Genomic DNA. Translation: AAD00923.1.
AF001990 Genomic DNA. Translation: AAD00924.1.
AF001991 Genomic DNA. Translation: AAD00925.1.
AF001992 Genomic DNA. Translation: AAD00926.1.
AF001993 Genomic DNA. Translation: AAD00927.1.
AF001994 Genomic DNA. Translation: AAD00928.1.
AF001995 Genomic DNA. Translation: AAD00929.1.
AF001996 Genomic DNA. Translation: AAD00930.1.
AF001997 Genomic DNA. Translation: AAD00931.1.
AF001998 Genomic DNA. Translation: AAD00932.1.
AF001999 Genomic DNA. Translation: AAD00933.1.
AF002000 Genomic DNA. Translation: AAD00934.1.
AF002001 Genomic DNA. Translation: AAD00935.1.
AF002002 Genomic DNA. Translation: AAD00936.1.
AF002003 Genomic DNA. Translation: AAD00937.1.
AF002004 Genomic DNA. Translation: AAD00938.1.
AF002005 Genomic DNA. Translation: AAD00939.1.
AF002006 Genomic DNA. Translation: AAD00940.1.
AF002007 Genomic DNA. Translation: AAD00941.1.
AF002008 Genomic DNA. Translation: AAD00942.1.
AF002009 Genomic DNA. Translation: AAD00943.1.
AF002010 Genomic DNA. Translation: AAD00944.1.
PIRiI54964.
RefSeqiNP_417213.1. NC_000913.3.
WP_001272928.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1E3MX-ray2.20A/B1-800[»]
1NG9X-ray2.60A/B1-800[»]
1OH5X-ray2.90A/B1-800[»]
1OH6X-ray2.40A/B1-800[»]
1OH7X-ray2.50A/B1-800[»]
1OH8X-ray2.90A/B1-800[»]
1W7AX-ray2.27A/B1-800[»]
1WB9X-ray2.10A/B1-800[»]
1WBBX-ray2.50A/B1-800[»]
1WBDX-ray2.40A/B1-800[»]
2OK2X-ray2.00A/B820-853[»]
2WTUX-ray3.40A/B1-800[»]
3K0SX-ray2.20A/B2-800[»]
3ZLJX-ray3.10A/B1-800[»]
C/D801-853[»]
5AKBX-ray4.71A/B/E1-800[»]
5AKCX-ray6.60A/B/E/F/I/J1-800[»]
5AKDX-ray7.60A/B/E/F/I/J1-800[»]
ProteinModelPortaliP23909.
SMRiP23909.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261421. 115 interactors.
DIPiDIP-10287N.
IntActiP23909. 10 interactors.
MINTiMINT-1264156.
STRINGi511145.b2733.

2D gel databases

SWISS-2DPAGEP23909.

Proteomic databases

PaxDbiP23909.
PRIDEiP23909.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75775; AAC75775; b2733.
BAE76810; BAE76810; BAE76810.
GeneIDi947206.
KEGGiecj:JW2703.
eco:b2733.
PATRICi32120868. VBIEscCol129921_2826.

Organism-specific databases

EchoBASEiEB0620.
EcoGeneiEG10625. mutS.

Phylogenomic databases

eggNOGiENOG4105D86. Bacteria.
COG0249. LUCA.
HOGENOMiHOG000221407.
InParanoidiP23909.
KOiK03555.
OMAiEMTEMAN.
PhylomeDBiP23909.

Enzyme and pathway databases

BioCyciEcoCyc:EG10625-MONOMER.
ECOL316407:JW2703-MONOMER.
MetaCyc:EG10625-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP23909.
PROiP23909.

Family and domain databases

Gene3Di3.30.420.110. 1 hit.
3.40.1170.10. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_00096. MutS. 1 hit.
InterProiIPR005748. DNA_mismatch_repair_MutS-1.
IPR007695. DNA_mismatch_repair_MutS-lik_N.
IPR000432. DNA_mismatch_repair_MutS_C.
IPR007861. DNA_mismatch_repair_MutS_clamp.
IPR007696. DNA_mismatch_repair_MutS_core.
IPR016151. DNA_mismatch_repair_MutS_N.
IPR007860. DNA_mmatch_repair_MutS_con_dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF01624. MutS_I. 1 hit.
PF05188. MutS_II. 1 hit.
PF05192. MutS_III. 1 hit.
PF05190. MutS_IV. 1 hit.
PF00488. MutS_V. 1 hit.
[Graphical view]
SMARTiSM00534. MUTSac. 1 hit.
SM00533. MUTSd. 1 hit.
[Graphical view]
SUPFAMiSSF48334. SSF48334. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF53150. SSF53150. 1 hit.
SSF55271. SSF55271. 1 hit.
TIGRFAMsiTIGR01070. mutS1. 1 hit.
PROSITEiPS00486. DNA_MISMATCH_REPAIR_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMUTS_ECOLI
AccessioniPrimary (citable) accession number: P23909
Secondary accession number(s): P71279, Q2MA96
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: March 1, 1992
Last modified: November 2, 2016
This is version 149 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.