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P23909

- MUTS_ECOLI

UniProt

P23909 - MUTS_ECOLI

Protein

DNA mismatch repair protein MutS

Gene

mutS

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 132 (01 Oct 2014)
      Sequence version 1 (01 Mar 1992)
      Previous versions | rss
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    Functioni

    This protein is involved in the repair of mismatches in DNA. It is possible that it carries out the mismatch recognition step. This protein has a weak ATPase activity.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi614 – 6218ATPSequence Analysis

    GO - Molecular functioni

    1. adenine/cytosine mispair binding Source: EcoliWiki
    2. ADP binding Source: EcoliWiki
    3. ATPase activity Source: EcoliWiki
    4. ATP binding Source: EcoliWiki
    5. damaged DNA binding Source: RefGenome
    6. DNA binding, bending Source: EcoliWiki
    7. DNA-dependent ATPase activity Source: RefGenome
    8. identical protein binding Source: IntAct
    9. mismatched DNA binding Source: EcoliWiki

    GO - Biological processi

    1. ATP catabolic process Source: GOC
    2. cellular response to DNA damage stimulus Source: EcoliWiki
    3. mismatch repair Source: EcoliWiki
    4. regulation of DNA recombination Source: EcoliWiki

    Keywords - Biological processi

    DNA damage, DNA repair

    Keywords - Ligandi

    ATP-binding, DNA-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciEcoCyc:EG10625-MONOMER.
    ECOL316407:JW2703-MONOMER.
    MetaCyc:EG10625-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA mismatch repair protein MutS
    Gene namesi
    Name:mutS
    Synonyms:fdv
    Ordered Locus Names:b2733, JW2703
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG10625. mutS.

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: EcoCyc
    2. mismatch repair complex Source: EcoliWiki

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 853853DNA mismatch repair protein MutSPRO_0000115094Add
    BLAST

    Proteomic databases

    PaxDbiP23909.
    PRIDEiP23909.

    2D gel databases

    SWISS-2DPAGEP23909.

    Expressioni

    Gene expression databases

    GenevestigatoriP23909.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    itself3EBI-554920,EBI-554920

    Protein-protein interaction databases

    DIPiDIP-10287N.
    IntActiP23909. 10 interactions.
    MINTiMINT-1264156.
    STRINGi511145.b2733.

    Structurei

    Secondary structure

    1
    853
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi7 – 93
    Helixi12 – 2312
    Beta strandi27 – 337
    Beta strandi36 – 405
    Helixi41 – 5111
    Beta strandi56 – 583
    Beta strandi61 – 633
    Beta strandi66 – 727
    Helixi73 – 753
    Helixi76 – 8510
    Beta strandi90 – 956
    Helixi99 – 1013
    Beta strandi103 – 1053
    Beta strandi108 – 1147
    Turni116 – 1183
    Helixi122 – 1243
    Beta strandi133 – 1386
    Beta strandi143 – 1486
    Turni150 – 1523
    Beta strandi155 – 1584
    Helixi163 – 17311
    Beta strandi176 – 1816
    Helixi187 – 1893
    Turni190 – 1923
    Beta strandi194 – 1996
    Helixi201 – 2044
    Helixi206 – 21712
    Beta strandi220 – 2223
    Helixi223 – 2253
    Helixi231 – 24818
    Beta strandi258 – 2603
    Helixi263 – 2653
    Helixi271 – 2766
    Beta strandi279 – 2813
    Beta strandi287 – 2904
    Helixi291 – 2955
    Helixi301 – 31212
    Helixi318 – 33013
    Helixi332 – 3343
    Helixi335 – 3439
    Helixi348 – 3569
    Helixi362 – 37211
    Helixi375 – 3839
    Helixi388 – 39710
    Helixi401 – 41010
    Beta strandi419 – 4224
    Helixi431 – 45828
    Beta strandi464 – 4685
    Turni469 – 4713
    Beta strandi472 – 4787
    Helixi479 – 4824
    Beta strandi490 – 4945
    Beta strandi496 – 5027
    Helixi504 – 53532
    Helixi536 – 5383
    Helixi539 – 56426
    Beta strandi570 – 5723
    Beta strandi574 – 5763
    Beta strandi578 – 5836
    Helixi588 – 5914
    Beta strandi592 – 5943
    Beta strandi599 – 6035
    Beta strandi605 – 6073
    Beta strandi609 – 6135
    Helixi620 – 63415
    Turni635 – 6373
    Beta strandi640 – 6478
    Beta strandi652 – 6576
    Helixi671 – 68313
    Beta strandi688 – 6947
    Beta strandi699 – 7024
    Helixi703 – 71715
    Beta strandi722 – 7265
    Helixi730 – 7345
    Helixi735 – 7384
    Beta strandi742 – 75211
    Beta strandi755 – 76511
    Helixi772 – 7787
    Helixi783 – 79715
    Helixi826 – 8316
    Turni834 – 8363
    Helixi839 – 85214

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1E3MX-ray2.20A/B1-800[»]
    1NG9X-ray2.60A/B1-800[»]
    1OH5X-ray2.90A/B1-800[»]
    1OH6X-ray2.40A/B1-800[»]
    1OH7X-ray2.50A/B1-800[»]
    1OH8X-ray2.90A/B1-800[»]
    1W7AX-ray2.27A/B1-800[»]
    1WB9X-ray2.10A/B1-800[»]
    1WBBX-ray2.50A/B1-800[»]
    1WBDX-ray2.40A/B1-800[»]
    2OK2X-ray2.00A/B820-853[»]
    2WTUX-ray3.40A/B1-800[»]
    3K0SX-ray2.20A/B2-800[»]
    3ZLJX-ray3.10A/B1-800[»]
    C/D801-853[»]
    ProteinModelPortaliP23909.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP23909.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the DNA mismatch repair MutS family.Curated

    Phylogenomic databases

    eggNOGiCOG0249.
    HOGENOMiHOG000221407.
    KOiK03555.
    OMAiSIAFMHA.
    OrthoDBiEOG6Q5NPV.
    PhylomeDBiP23909.

    Family and domain databases

    Gene3Di3.30.420.110. 1 hit.
    3.40.1170.10. 1 hit.
    3.40.50.300. 1 hit.
    HAMAPiMF_00096. MutS.
    InterProiIPR005748. DNA_mismatch_repair_MutS-1.
    IPR007695. DNA_mismatch_repair_MutS-lik_N.
    IPR000432. DNA_mismatch_repair_MutS_C.
    IPR007861. DNA_mismatch_repair_MutS_clamp.
    IPR007696. DNA_mismatch_repair_MutS_core.
    IPR016151. DNA_mismatch_repair_MutS_N.
    IPR007860. DNA_mmatch_repair_MutS_con_dom.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PfamiPF01624. MutS_I. 1 hit.
    PF05188. MutS_II. 1 hit.
    PF05192. MutS_III. 1 hit.
    PF05190. MutS_IV. 1 hit.
    PF00488. MutS_V. 1 hit.
    [Graphical view]
    SMARTiSM00534. MUTSac. 1 hit.
    SM00533. MUTSd. 1 hit.
    [Graphical view]
    SUPFAMiSSF48334. SSF48334. 1 hit.
    SSF52540. SSF52540. 1 hit.
    SSF53150. SSF53150. 1 hit.
    SSF55271. SSF55271. 1 hit.
    TIGRFAMsiTIGR01070. mutS1. 1 hit.
    PROSITEiPS00486. DNA_MISMATCH_REPAIR_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P23909-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSAIENFDAH TPMMQQYLRL KAQHPEILLF YRMGDFYELF YDDAKRASQL    50
    LDISLTKRGA SAGEPIPMAG IPYHAVENYL AKLVNQGESV AICEQIGDPA 100
    TSKGPVERKV VRIVTPGTIS DEALLQERQD NLLAAIWQDS KGFGYATLDI 150
    SSGRFRLSEP ADRETMAAEL QRTNPAELLY AEDFAEMSLI EGRRGLRRRP 200
    LWEFEIDTAR QQLNLQFGTR DLVGFGVENA PRGLCAAGCL LQYAKDTQRT 250
    TLPHIRSITM EREQDSIIMD AATRRNLEIT QNLAGGAENT LASVLDCTVT 300
    PMGSRMLKRW LHMPVRDTRV LLERQQTIGA LQDFTAGLQP VLRQVGDLER 350
    ILARLALRTA RPRDLARMRH AFQQLPELRA QLETVDSAPV QALREKMGEF 400
    AELRDLLERA IIDTPPVLVR DGGVIASGYN EELDEWRALA DGATDYLERL 450
    EVRERERTGL DTLKVGFNAV HGYYIQISRG QSHLAPINYM RRQTLKNAER 500
    YIIPELKEYE DKVLTSKGKA LALEKQLYEE LFDLLLPHLE ALQQSASALA 550
    ELDVLVNLAE RAYTLNYTCP TFIDKPGIRI TEGRHPVVEQ VLNEPFIANP 600
    LNLSPQRRML IITGPNMGGK STYMRQTALI ALMAYIGSYV PAQKVEIGPI 650
    DRIFTRVGAA DDLASGRSTF MVEMTETANI LHNATEYSLV LMDEIGRGTS 700
    TYDGLSLAWA CAENLANKIK ALTLFATHYF ELTQLPEKME GVANVHLDAL 750
    EHGDTIAFMH SVQDGAASKS YGLAVAALAG VPKEVIKRAR QKLRELESIS 800
    PNAAATQVDG TQMSLLSVPE ETSPAVEALE NLDPDSLTPR QALEWIYRLK 850
    SLV 853
    Length:853
    Mass (Da):95,247
    Last modified:March 1, 1992 - v1
    Checksum:i5A1F295362671D55
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M64730 Genomic DNA. Translation: AAA24188.1.
    U29579 Genomic DNA. Translation: AAA69243.1.
    U00096 Genomic DNA. Translation: AAC75775.1.
    AP009048 Genomic DNA. Translation: BAE76810.1.
    AF004287 Genomic DNA. Translation: AAD01197.1.
    AF001987 Genomic DNA. Translation: AAD00921.1.
    AF001988 Genomic DNA. Translation: AAD00922.1.
    AF001989 Genomic DNA. Translation: AAD00923.1.
    AF001990 Genomic DNA. Translation: AAD00924.1.
    AF001991 Genomic DNA. Translation: AAD00925.1.
    AF001992 Genomic DNA. Translation: AAD00926.1.
    AF001993 Genomic DNA. Translation: AAD00927.1.
    AF001994 Genomic DNA. Translation: AAD00928.1.
    AF001995 Genomic DNA. Translation: AAD00929.1.
    AF001996 Genomic DNA. Translation: AAD00930.1.
    AF001997 Genomic DNA. Translation: AAD00931.1.
    AF001998 Genomic DNA. Translation: AAD00932.1.
    AF001999 Genomic DNA. Translation: AAD00933.1.
    AF002000 Genomic DNA. Translation: AAD00934.1.
    AF002001 Genomic DNA. Translation: AAD00935.1.
    AF002002 Genomic DNA. Translation: AAD00936.1.
    AF002003 Genomic DNA. Translation: AAD00937.1.
    AF002004 Genomic DNA. Translation: AAD00938.1.
    AF002005 Genomic DNA. Translation: AAD00939.1.
    AF002006 Genomic DNA. Translation: AAD00940.1.
    AF002007 Genomic DNA. Translation: AAD00941.1.
    AF002008 Genomic DNA. Translation: AAD00942.1.
    AF002009 Genomic DNA. Translation: AAD00943.1.
    AF002010 Genomic DNA. Translation: AAD00944.1.
    PIRiI54964.
    RefSeqiNP_417213.1. NC_000913.3.
    YP_490942.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC75775; AAC75775; b2733.
    BAE76810; BAE76810; BAE76810.
    GeneIDi12932427.
    947206.
    KEGGiecj:Y75_p2671.
    eco:b2733.
    PATRICi32120868. VBIEscCol129921_2826.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M64730 Genomic DNA. Translation: AAA24188.1 .
    U29579 Genomic DNA. Translation: AAA69243.1 .
    U00096 Genomic DNA. Translation: AAC75775.1 .
    AP009048 Genomic DNA. Translation: BAE76810.1 .
    AF004287 Genomic DNA. Translation: AAD01197.1 .
    AF001987 Genomic DNA. Translation: AAD00921.1 .
    AF001988 Genomic DNA. Translation: AAD00922.1 .
    AF001989 Genomic DNA. Translation: AAD00923.1 .
    AF001990 Genomic DNA. Translation: AAD00924.1 .
    AF001991 Genomic DNA. Translation: AAD00925.1 .
    AF001992 Genomic DNA. Translation: AAD00926.1 .
    AF001993 Genomic DNA. Translation: AAD00927.1 .
    AF001994 Genomic DNA. Translation: AAD00928.1 .
    AF001995 Genomic DNA. Translation: AAD00929.1 .
    AF001996 Genomic DNA. Translation: AAD00930.1 .
    AF001997 Genomic DNA. Translation: AAD00931.1 .
    AF001998 Genomic DNA. Translation: AAD00932.1 .
    AF001999 Genomic DNA. Translation: AAD00933.1 .
    AF002000 Genomic DNA. Translation: AAD00934.1 .
    AF002001 Genomic DNA. Translation: AAD00935.1 .
    AF002002 Genomic DNA. Translation: AAD00936.1 .
    AF002003 Genomic DNA. Translation: AAD00937.1 .
    AF002004 Genomic DNA. Translation: AAD00938.1 .
    AF002005 Genomic DNA. Translation: AAD00939.1 .
    AF002006 Genomic DNA. Translation: AAD00940.1 .
    AF002007 Genomic DNA. Translation: AAD00941.1 .
    AF002008 Genomic DNA. Translation: AAD00942.1 .
    AF002009 Genomic DNA. Translation: AAD00943.1 .
    AF002010 Genomic DNA. Translation: AAD00944.1 .
    PIRi I54964.
    RefSeqi NP_417213.1. NC_000913.3.
    YP_490942.1. NC_007779.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1E3M X-ray 2.20 A/B 1-800 [» ]
    1NG9 X-ray 2.60 A/B 1-800 [» ]
    1OH5 X-ray 2.90 A/B 1-800 [» ]
    1OH6 X-ray 2.40 A/B 1-800 [» ]
    1OH7 X-ray 2.50 A/B 1-800 [» ]
    1OH8 X-ray 2.90 A/B 1-800 [» ]
    1W7A X-ray 2.27 A/B 1-800 [» ]
    1WB9 X-ray 2.10 A/B 1-800 [» ]
    1WBB X-ray 2.50 A/B 1-800 [» ]
    1WBD X-ray 2.40 A/B 1-800 [» ]
    2OK2 X-ray 2.00 A/B 820-853 [» ]
    2WTU X-ray 3.40 A/B 1-800 [» ]
    3K0S X-ray 2.20 A/B 2-800 [» ]
    3ZLJ X-ray 3.10 A/B 1-800 [» ]
    C/D 801-853 [» ]
    ProteinModelPortali P23909.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-10287N.
    IntActi P23909. 10 interactions.
    MINTi MINT-1264156.
    STRINGi 511145.b2733.

    2D gel databases

    SWISS-2DPAGE P23909.

    Proteomic databases

    PaxDbi P23909.
    PRIDEi P23909.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC75775 ; AAC75775 ; b2733 .
    BAE76810 ; BAE76810 ; BAE76810 .
    GeneIDi 12932427.
    947206.
    KEGGi ecj:Y75_p2671.
    eco:b2733.
    PATRICi 32120868. VBIEscCol129921_2826.

    Organism-specific databases

    EchoBASEi EB0620.
    EcoGenei EG10625. mutS.

    Phylogenomic databases

    eggNOGi COG0249.
    HOGENOMi HOG000221407.
    KOi K03555.
    OMAi SIAFMHA.
    OrthoDBi EOG6Q5NPV.
    PhylomeDBi P23909.

    Enzyme and pathway databases

    BioCyci EcoCyc:EG10625-MONOMER.
    ECOL316407:JW2703-MONOMER.
    MetaCyc:EG10625-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P23909.
    PROi P23909.

    Gene expression databases

    Genevestigatori P23909.

    Family and domain databases

    Gene3Di 3.30.420.110. 1 hit.
    3.40.1170.10. 1 hit.
    3.40.50.300. 1 hit.
    HAMAPi MF_00096. MutS.
    InterProi IPR005748. DNA_mismatch_repair_MutS-1.
    IPR007695. DNA_mismatch_repair_MutS-lik_N.
    IPR000432. DNA_mismatch_repair_MutS_C.
    IPR007861. DNA_mismatch_repair_MutS_clamp.
    IPR007696. DNA_mismatch_repair_MutS_core.
    IPR016151. DNA_mismatch_repair_MutS_N.
    IPR007860. DNA_mmatch_repair_MutS_con_dom.
    IPR027417. P-loop_NTPase.
    [Graphical view ]
    Pfami PF01624. MutS_I. 1 hit.
    PF05188. MutS_II. 1 hit.
    PF05192. MutS_III. 1 hit.
    PF05190. MutS_IV. 1 hit.
    PF00488. MutS_V. 1 hit.
    [Graphical view ]
    SMARTi SM00534. MUTSac. 1 hit.
    SM00533. MUTSd. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48334. SSF48334. 1 hit.
    SSF52540. SSF52540. 1 hit.
    SSF53150. SSF53150. 1 hit.
    SSF55271. SSF55271. 1 hit.
    TIGRFAMsi TIGR01070. mutS1. 1 hit.
    PROSITEi PS00486. DNA_MISMATCH_REPAIR_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The Escherichia coli fdv gene probably encodes mutS and is located at minute 58.8 adjacent to the hyc-hyp gene cluster."
      Schlensog V., Boeck A.
      J. Bacteriol. 173:7414-7415(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    4. Denamur E.
      Submitted (MAY-1997) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 602-731.
      Strain: Various strains.
    5. "Escherichia coli proteome analysis using the gene-protein database."
      VanBogelen R.A., Abshire K.Z., Moldover B., Olson E.R., Neidhardt F.C.
      Electrophoresis 18:1243-1251(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY 2D-GEL.

    Entry informationi

    Entry nameiMUTS_ECOLI
    AccessioniPrimary (citable) accession number: P23909
    Secondary accession number(s): P71279, Q2MA96
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 1, 1992
    Last sequence update: March 1, 1992
    Last modified: October 1, 2014
    This is version 132 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3