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Protein

Acetylornithine deacetylase

Gene

argE

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Displays a broad specificity and can also deacylate substrates such as acetylarginine, acetylhistidine or acetylglutamate semialdehyde.

Catalytic activityi

N(2)-acetyl-L-ornithine + H2O = acetate + L-ornithine.

Cofactori

Protein has several cofactor binding sites:

Pathwayi: L-arginine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-ornithine from N(2)-acetyl-L-ornithine (linear).
Proteins known to be involved in this subpathway in this organism are:
  1. Acetylornithine deacetylase (argE)
This subpathway is part of the pathway L-arginine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-ornithine from N(2)-acetyl-L-ornithine (linear), the pathway L-arginine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi80 – 801Cobalt or zinc 1By similarity
Active sitei82 – 821By similarity
Metal bindingi112 – 1121Cobalt or zinc 1By similarity
Metal bindingi112 – 1121Cobalt or zinc 2By similarity
Active sitei144 – 1441Proton acceptorBy similarity
Metal bindingi145 – 1451Cobalt or zinc 2By similarity
Metal bindingi169 – 1691Cobalt or zinc 1By similarity
Metal bindingi355 – 3551Cobalt or zinc 2By similarity

GO - Molecular functioni

  • acetylornithine deacetylase activity Source: EcoliWiki
  • cobalt ion binding Source: EcoCyc
  • metallopeptidase activity Source: InterPro
  • zinc ion binding Source: EcoCyc

GO - Biological processi

  • arginine biosynthetic process Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Amino-acid biosynthesis, Arginine biosynthesis

Keywords - Ligandi

Cobalt, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciEcoCyc:ACETYLORNDEACET-MONOMER.
ECOL316407:JW3929-MONOMER.
MetaCyc:ACETYLORNDEACET-MONOMER.
BRENDAi3.5.1.16. 2026.
UniPathwayiUPA00068; UER00110.

Protein family/group databases

MEROPSiM20.974.

Names & Taxonomyi

Protein namesi
Recommended name:
Acetylornithine deacetylase (EC:3.5.1.16)
Short name:
AO
Short name:
Acetylornithinase
Alternative name(s):
N-acetylornithinase
Short name:
NAO
Gene namesi
Name:argE
Ordered Locus Names:b3957, JW3929
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11286. argE.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 383383Acetylornithine deacetylasePRO_0000185240Add
BLAST

Proteomic databases

EPDiP23908.
PaxDbiP23908.
PRIDEiP23908.

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

BioGridi4259568. 12 interactions.
STRINGi511145.b3957.

Structurei

3D structure databases

ProteinModelPortaliP23908.
SMRiP23908. Positions 1-367.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M20A family. ArgE subfamily.Curated

Phylogenomic databases

eggNOGiENOG4105CWC. Bacteria.
COG0624. LUCA.
HOGENOMiHOG000243769.
InParanoidiP23908.
KOiK01438.
OMAiGTEAPYL.
PhylomeDBiP23908.

Family and domain databases

CDDicd03894. M20_ArgE. 1 hit.
Gene3Di3.30.70.360. 1 hit.
HAMAPiMF_01108. ArgE. 1 hit.
InterProiIPR010169. AcOrn-deacetyl.
IPR001261. ArgE/DapE_CS.
IPR002933. Peptidase_M20.
IPR011650. Peptidase_M20_dimer.
[Graphical view]
PfamiPF07687. M20_dimer. 1 hit.
PF01546. Peptidase_M20. 1 hit.
[Graphical view]
SUPFAMiSSF55031. SSF55031. 1 hit.
TIGRFAMsiTIGR01892. AcOrn-deacetyl. 1 hit.
PROSITEiPS00758. ARGE_DAPE_CPG2_1. 1 hit.
PS00759. ARGE_DAPE_CPG2_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P23908-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKNKLPPFIE IYRALIATPS ISATEEALDQ SNADLITLLA DWFKDLGFNV
60 70 80 90 100
EVQPVPGTRN KFNMLASIGQ GAGGLLLAGH TDTVPFDDGR WTRDPFTLTE
110 120 130 140 150
HDGKLYGLGT ADMKGFFAFI LDALRDVDVT KLKKPLYILA TADEETSMAG
160 170 180 190 200
ARYFAETTAL RPDCAIIGEP TSLQPVRAHK GHISNAIRIQ GQSGHSSDPA
210 220 230 240 250
RGVNAIELMH DAIGHILQLR DNLKERYHYE AFTVPYPTLN LGHIHGGDAS
260 270 280 290 300
NRICACCELH MDIRPLPGMT LNELNGLLND ALAPVSERWP GRLTVDELHP
310 320 330 340 350
PIPGYECPPN HQLVEVVEKL LGAKTEVVNY CTEAPFIQTL CPTLVLGPGS
360 370 380
INQAHQPDEY LETRFIKPTR ELITQVIHHF CWH
Length:383
Mass (Da):42,347
Last modified:December 1, 1992 - v2
Checksum:iAFE04B99B296540B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X55417 Genomic DNA. Translation: CAA39076.1.
X62807 Genomic DNA. Translation: CAA44625.1.
AY330219 Genomic DNA. Translation: AAP92671.1.
AY331711 Genomic DNA. Translation: AAP92815.1.
U00006 Genomic DNA. Translation: AAC43063.1.
U00096 Genomic DNA. Translation: AAC76939.1.
AP009048 Genomic DNA. Translation: BAE77354.1.
J01587 Genomic DNA. Translation: AAB59145.1.
J01589 Genomic DNA. Translation: AAA23484.2.
PIRiB42377.
RefSeqiNP_418392.1. NC_000913.3.
WP_001298964.1. NZ_CP014272.1.

Genome annotation databases

EnsemblBacteriaiAAC76939; AAC76939; b3957.
BAE77354; BAE77354; BAE77354.
GeneIDi948456.
KEGGiecj:JW3929.
eco:b3957.
PATRICi32123435. VBIEscCol129921_4078.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X55417 Genomic DNA. Translation: CAA39076.1.
X62807 Genomic DNA. Translation: CAA44625.1.
AY330219 Genomic DNA. Translation: AAP92671.1.
AY331711 Genomic DNA. Translation: AAP92815.1.
U00006 Genomic DNA. Translation: AAC43063.1.
U00096 Genomic DNA. Translation: AAC76939.1.
AP009048 Genomic DNA. Translation: BAE77354.1.
J01587 Genomic DNA. Translation: AAB59145.1.
J01589 Genomic DNA. Translation: AAA23484.2.
PIRiB42377.
RefSeqiNP_418392.1. NC_000913.3.
WP_001298964.1. NZ_CP014272.1.

3D structure databases

ProteinModelPortaliP23908.
SMRiP23908. Positions 1-367.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259568. 12 interactions.
STRINGi511145.b3957.

Protein family/group databases

MEROPSiM20.974.

Proteomic databases

EPDiP23908.
PaxDbiP23908.
PRIDEiP23908.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76939; AAC76939; b3957.
BAE77354; BAE77354; BAE77354.
GeneIDi948456.
KEGGiecj:JW3929.
eco:b3957.
PATRICi32123435. VBIEscCol129921_4078.

Organism-specific databases

EchoBASEiEB1263.
EcoGeneiEG11286. argE.

Phylogenomic databases

eggNOGiENOG4105CWC. Bacteria.
COG0624. LUCA.
HOGENOMiHOG000243769.
InParanoidiP23908.
KOiK01438.
OMAiGTEAPYL.
PhylomeDBiP23908.

Enzyme and pathway databases

UniPathwayiUPA00068; UER00110.
BioCyciEcoCyc:ACETYLORNDEACET-MONOMER.
ECOL316407:JW3929-MONOMER.
MetaCyc:ACETYLORNDEACET-MONOMER.
BRENDAi3.5.1.16. 2026.

Miscellaneous databases

PROiP23908.

Family and domain databases

CDDicd03894. M20_ArgE. 1 hit.
Gene3Di3.30.70.360. 1 hit.
HAMAPiMF_01108. ArgE. 1 hit.
InterProiIPR010169. AcOrn-deacetyl.
IPR001261. ArgE/DapE_CS.
IPR002933. Peptidase_M20.
IPR011650. Peptidase_M20_dimer.
[Graphical view]
PfamiPF07687. M20_dimer. 1 hit.
PF01546. Peptidase_M20. 1 hit.
[Graphical view]
SUPFAMiSSF55031. SSF55031. 1 hit.
TIGRFAMsiTIGR01892. AcOrn-deacetyl. 1 hit.
PROSITEiPS00758. ARGE_DAPE_CPG2_1. 1 hit.
PS00759. ARGE_DAPE_CPG2_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiARGE_ECOLI
AccessioniPrimary (citable) accession number: P23908
Secondary accession number(s): Q2M8Q2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: December 1, 1992
Last modified: September 7, 2016
This is version 146 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. Peptidase families
    Classification of peptidase families and list of entries
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.