Reviewed,
UniProtKB/Swiss-Prot P23908 (ARGE_ECOLI)
Last modified
February 9, 2010.
Version 97.
History...
Clusters with 100%,
90%,
50% identity |
Documents (4) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Acetylornithine deacetylase Short name=Acetylornithinase Short name=AO EC=3.5.1.16 Alternative name(s): N-acetylornithinase Short name=NAO | ||||
| Gene names |
| ||||
| Organism | Escherichia coli (strain K12) [Complete proteome] [HAMAP] | ||||
| Taxonomic identifier | 83333 [NCBI] | ||||
| Taxonomic lineage | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia |
Protein attributes
| Sequence length | 383 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Displays a broad specificity and can also deacylate substrates such as acetylarginine, acetylhistidine or acetylglutamate semialdehyde. HAMAP MF_01108 |
| Catalytic activity | N(2)-acetyl-L-ornithine + H2O = acetate + L-ornithine. HAMAP MF_01108 |
| Cofactor | Binds 2 zinc or cobalt ions per subunit. HAMAP MF_01108 Glutathione. HAMAP MF_01108 |
| Pathway | Amino-acid biosynthesis; L-arginine biosynthesis; L-ornithine from N(2)-acetyl-L-ornithine (linear): step 1/1. HAMAP MF_01108 |
| Subunit structure | Homodimer. HAMAP MF_01108 |
| Subcellular location | Cytoplasm Probable HAMAP MF_01108. |
| Sequence similarities | Belongs to the peptidase M20A family. ArgE subfamily. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Amino-acid biosynthesis Arginine biosynthesis |
| Cellular component | Cytoplasm |
| Ligand | Cobalt Metal-binding Zinc |
| Molecular function | Hydrolase |
| Technical term | Complete proteome |
| Gene Ontology (GO) | |
| Biological process | arginine biosynthetic process Inferred from electronic annotation. Source: HAMAP proteolysisInferred from electronic annotation. Source: InterPro |
| Cellular component | cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular function | acetylornithine deacetylase activity Ref.1 Inferred from direct assay. Source: UniProtKB cobalt ion bindingInferred from electronic annotation. Source: UniProtKB-KW metallopeptidase activityInferred from electronic annotation. Source: InterPro protein dimerization activityInferred from electronic annotation. Source: InterPro zinc ion bindingInferred from electronic annotation. Source: HAMAP |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 383 | 383 | Acetylornithine deacetylase HAMAP MF_01108 | PRO_0000185240 | |||||
Sites | |||||||||
| Active site | 82 | 1 | By similarity | ||||||
| Active site | 144 | 1 | Proton acceptor By similarity | ||||||
| Metal binding | 80 | 1 | Cobalt or zinc 1 By similarity | ||||||
| Metal binding | 112 | 1 | Cobalt or zinc 1 By similarity | ||||||
| Metal binding | 112 | 1 | Cobalt or zinc 2 By similarity | ||||||
| Metal binding | 145 | 1 | Cobalt or zinc 2 By similarity | ||||||
| Metal binding | 169 | 1 | Cobalt or zinc 1 By similarity | ||||||
| Metal binding | 355 | 1 | Cobalt or zinc 2 By similarity | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Structural and biochemical characterization of the Escherichia coli argE gene product." Meinnel T., Schmitt E., Mechulam Y., Blanquet S. J. Bacteriol. 174:2323-2331(1992) [PubMed: 1551850] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12. |
| [2] | "Acetylornithine deacetylase, succinyldiaminopimelate desuccinylase and carboxypeptidase G2 are evolutionarily related." Boyen A., Charlier D.R.M., Sakanyan V., Mett I., Glansdorff N. Gene 116:1-6(1992) [PubMed: 1628835] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12. |
| [3] | Xiao X.G., Cao K.H. Submitted (JUN-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12 / DH5-alpha and K12 / JM101 / ATCC 33876 / DSM 3948. |
| [4] | "Analysis of the Escherichia coli genome. IV. DNA sequence of the region from 89.2 to 92.8 minutes." Blattner F.R., Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L. Nucleic Acids Res. 21:5408-5417(1993) [PubMed: 8265357] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076. |
| [5] | "The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076. |
| [6] | "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911. |
| [7] | "The regulatory region of the divergent argECBH operon in Escherichia coli K-12." Piette J., Cunin R., Boyen A., Charlier D.R.M., Crabeel M., van Vliet F., Glansdorff N., Squires C., Squires C.L. Nucleic Acids Res. 10:8031-8048(1982) [PubMed: 6761650] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-11. Strain: K12. |
| [8] | "IS3 can function as a mobile promoter in E. coli." Charlier D.R.M., Piette J., Glansdorff N. Nucleic Acids Res. 10:5935-5948(1982) [PubMed: 6292860] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-11. |
| [9] | "Mechanistic analysis of the argE-encoded N-acetylornithine deacetylase." Javid-Majd F., Blanchard J.S. Biochemistry 39:1285-1293(2000) [PubMed: 10684608] [Abstract] Cited for: CHARACTERIZATION. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | X55417 Genomic DNA. Translation: CAA39076.1. X62807 Genomic DNA. Translation: CAA44625.1. AY330219 Genomic DNA. Translation: AAP92671.1. AY331711 Genomic DNA. Translation: AAP92815.1. U00006 Genomic DNA. Translation: AAC43063.1. U00096 Genomic DNA. Translation: AAC76939.1. AP009048 Genomic DNA. Translation: BAE77354.1. J01587 Genomic DNA. Translation: AAB59145.1. J01589 Genomic DNA. Translation: AAA23484.2. |
| PIR | B42377. |
| RefSeq | AP_003853.1. NP_418392.1. |
3D structure databases | |
| SMR | P23908. Positions 1-378. |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | P23908. |
Genome annotation databases | |
| GeneID | 948456. |
| GenomeReviews | Gene locus JW3929 in contig AP009048_GR. Gene locus b3957 in contig U00096_GR. |
| KEGG | ecj:JW3929. eco:b3957. |
Organism-specific databases | |
| EchoBASE | EB1263. |
| EcoGene | EG11286. argE. |
| CMR | Search... |
Phylogenomic databases | |
| eggNOG | COG0624. |
| HOGENOM | HBG728841. |
| OMA | ILATCDE. |
Enzyme and pathway databases | |
| BioCyc | EcoCyc:ACETYLORNDEACET-MONOMER. ECOL168927:B3957-MONOMER. MetaCyc:ACETYLORNDEACET-MONOMER. |
Gene expression databases | |
| Genevestigator | P23908. |
Family and domain databases | |
| HAMAP | MF_01108. ArgE. [Tree] |
| InterPro | IPR010169. AcOrn-deacetyl. IPR001261. ArgE/DapE_CS. IPR002933. Peptidase_M20. IPR011650. Peptidase_M20_dimer. [Graphical view] |
| Pfam | PF07687. M20_dimer. 1 hit. PF01546. Peptidase_M20. 1 hit. [Graphical view] |
| TIGRFAMs | TIGR01892. AcOrn-deacetyl. 1 hit. |
| PROSITE | PS00758. ARGE_DAPE_CPG2_1. 1 hit. PS00759. ARGE_DAPE_CPG2_2. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | ARGE_ECOLI | ||||||||
| Accession | Primary (citable) accession number: P23908 Secondary accession number(s): Q2M8Q2 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes) | ||||||||
Relevant documents
| Escherichia coli Escherichia coli (strain K12): entries and cross-references to EcoGene |
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| Peptidase families Classification of peptidase families and list of entries |
| SIMILARITY comments Index of protein domains and families |

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