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P23906 (IRF2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 123. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Interferon regulatory factor 2

Short name=IRF-2
Gene names
Name:Irf2
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length349 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Specifically binds to the upstream regulatory region of type I IFN and IFN-inducible MHC class I genes (the interferon consensus sequence (ICS)) and represses those genes. Also acts as an activator for several genes including H4 and IL7. Constitutively binds to the ISRE promoter to activate IL7. Involved in cell cycle regulation through binding the site II (HiNF-M) promoter region of H4 and activating transcription during cell growth. Antagonizes IRF1 transcriptional activation.

Subunit structure

Interacts with BRD7, IRF2BP1 and IRF2BP2. Interacts with CREBBP in growing cells; the interaction acetylates IRF2 and regulates IRF2-dependent H4 promoter activity By similarity.

Subcellular location

Nucleus.

Induction

By viruses and IFN.

Post-translational modification

Acetylated by CBP/ p300 during cell-growth. Acetylation on Lys-75 is required for stimulation of H4 promoter activity By similarity.

The major sites of sumoylation are Lys-137 and Lys-293. Sumoylation with SUMO1 increases its transcriptional repressor activity on IRF1 and diminishes its ability to activate ISRE and H4 promoter By similarity.

Sequence similarities

Belongs to the IRF family.

Contains 1 IRF tryptophan pentad repeat DNA-binding domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 349349Interferon regulatory factor 2
PRO_0000154550

Regions

DNA binding5 – 113109IRF tryptophan pentad repeat

Amino acid modifications

Modified residue751N6-acetyllysine By similarity
Modified residue781N6-acetyllysine By similarity
Cross-link137Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO) By similarity
Cross-link166Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO) By similarity
Cross-link293Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO) By similarity

Secondary structure

....................... 349
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P23906 [UniParc].

Last modified November 1, 1991. Version 1.
Checksum: 8738B082FB40FB11

FASTA34939,453
        10         20         30         40         50         60 
MPVERMRMRP WLEEQINSNT IPGLKWLNKE KKIFQIPWMH AARHGWDVEK DAPLFRNWAI 

        70         80         90        100        110        120 
HTGKHQPGID KPDPKTWKAN FRCAMNSLPD IEEVKDRSIK KGNNAFRVYR MLPLSERPSK 

       130        140        150        160        170        180 
KGKKPKTEKE ERVKHIKQEP VESSLGLSNG VSGFSPEYAV LTSAIKNEVD STVNIIVVGQ 

       190        200        210        220        230        240 
SHLDSNIEDQ EIVTNPPDIC QVVEVTTESD DQPVSMSELY PLQISPVSSY AESETTDSVA 

       250        260        270        280        290        300 
SDEENAEGRP HWRKRSIEGK QYLSNMGTRN TYLLPSMATF VTSNKPDLQV TIKEDSCPMP 

       310        320        330        340 
YNSSWPPFTD LPLPAPVTPT PSSSRPDRET RASVIKKTSD ITQARVKSC 

« Hide

References

[1]"Structurally similar but functionally distinct factors, IRF-1 and IRF-2, bind to the same regulatory elements of IFN and IFN-inducible genes."
Harada H., Fujita T., Miyamoto M., Kimura Y., Maruyama M., Furia A., Miyata T., Taniguchi T.
Cell 58:729-739(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]Lubec G., Sunyer B., Chen W.-Q.
Submitted (JAN-2009) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 108-120, IDENTIFICATION BY MASS SPECTROMETRY.
Strain: OF1.
Tissue: Hippocampus.
[3]"Solution structure of the IRF-2 DNA-binding domain: a novel subgroup of the winged helix-turn-helix family."
Furui J., Uegaki K., Yamazaki T., Shirakawa M., Swindells M.B., Harada H., Taniguchi T., Kyogoku Y.
Structure 6:491-500(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: STRUCTURE BY NMR OF 2-113.
[4]"Crystal structure of an IRF-DNA complex reveals novel DNA recognition and cooperative binding to a tandem repeat of core sequences."
Fujii Y., Shimizu T., Kusumoto M., Kyogoku Y., Taniguchi T., Hakoshima T.
EMBO J. 18:5028-5041(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS) OF 1-113.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
J03168 mRNA. Translation: AAA39333.1.
CCDSCCDS22295.1.
RefSeqNP_032417.3. NM_008391.4.
XP_006509351.1. XM_006509288.1.
XP_006509352.1. XM_006509289.1.
UniGeneMm.1149.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1IRFNMR-A2-113[»]
1IRGNMR-A2-113[»]
2IRFX-ray2.20G/H/I/J/K/L1-113[»]
ProteinModelPortalP23906.
SMRP23906. Positions 5-113.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid200785. 1 interaction.
IntActP23906. 1 interaction.
MINTMINT-4098768.

PTM databases

PhosphoSiteP23906.

Proteomic databases

PaxDbP23906.
PRIDEP23906.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000034041; ENSMUSP00000034041; ENSMUSG00000031627.
GeneID16363.
KEGGmmu:16363.
UCSCuc009lqo.2. mouse.

Organism-specific databases

CTD3660.
MGIMGI:96591. Irf2.

Phylogenomic databases

eggNOGNOG41436.
HOGENOMHOG000037937.
HOVERGENHBG003455.
InParanoidP23906.
KOK10153.
OMASWPPFPD.
OrthoDBEOG72ZCFD.
PhylomeDBP23906.
TreeFamTF328512.

Gene expression databases

ArrayExpressP23906.
BgeeP23906.
CleanExMM_IRF2.
GenevestigatorP23906.

Family and domain databases

Gene3D1.10.10.10. 1 hit.
InterProIPR017431. Interferon_reg_fac-1/2.
IPR019817. Interferon_reg_fac_CS.
IPR001346. Interferon_reg_fact_DNA-bd_dom.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamPF00605. IRF. 1 hit.
[Graphical view]
PIRSFPIRSF038196. IFN_RF1/2. 1 hit.
PRINTSPR00267. INTFRNREGFCT.
SMARTSM00348. IRF. 1 hit.
[Graphical view]
PROSITEPS00601. IRF_1. 1 hit.
PS51507. IRF_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP23906.
NextBio289470.
PROP23906.
SOURCESearch...

Entry information

Entry nameIRF2_MOUSE
AccessionPrimary (citable) accession number: P23906
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: November 1, 1991
Last modified: July 9, 2014
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot