SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P23906

- IRF2_MOUSE

UniProt

P23906 - IRF2_MOUSE

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Interferon regulatory factor 2
Gene
Irf2
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Specifically binds to the upstream regulatory region of type I IFN and IFN-inducible MHC class I genes (the interferon consensus sequence (ICS)) and represses those genes. Also acts as an activator for several genes including H4 and IL7. Constitutively binds to the ISRE promoter to activate IL7. Involved in cell cycle regulation through binding the site II (HiNF-M) promoter region of H4 and activating transcription during cell growth. Antagonizes IRF1 transcriptional activation.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi5 – 113109IRF tryptophan pentad repeat
Add
BLAST

GO - Molecular functioni

  1. regulatory region DNA binding Source: InterPro
  2. sequence-specific DNA binding transcription factor activity Source: InterPro

GO - Biological processi

  1. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator, Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

ReactomeiREACT_198521. TRAF6 mediated IRF7 activation.
REACT_198649. Factors involved in megakaryocyte development and platelet production.
REACT_198660. Interferon gamma signaling.

Names & Taxonomyi

Protein namesi
Recommended name:
Interferon regulatory factor 2
Short name:
IRF-2
Gene namesi
Name:Irf2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 8

Organism-specific databases

MGIiMGI:96591. Irf2.

Subcellular locationi

GO - Cellular componenti

  1. focal adhesion Source: Ensembl
  2. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 349349Interferon regulatory factor 2
PRO_0000154550Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei75 – 751N6-acetyllysine By similarity
Modified residuei78 – 781N6-acetyllysine By similarity
Cross-linki137 – 137Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO) By similarity
Cross-linki166 – 166Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO) By similarity
Cross-linki293 – 293Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO) By similarity

Post-translational modificationi

Acetylated by CBP/ p300 during cell-growth. Acetylation on Lys-75 is required for stimulation of H4 promoter activity By similarity.
The major sites of sumoylation are Lys-137 and Lys-293. Sumoylation with SUMO1 increases its transcriptional repressor activity on IRF1 and diminishes its ability to activate ISRE and H4 promoter By similarity.

Keywords - PTMi

Acetylation, Isopeptide bond, Ubl conjugation

Proteomic databases

PaxDbiP23906.
PRIDEiP23906.

PTM databases

PhosphoSiteiP23906.

Expressioni

Inductioni

By viruses and IFN.

Gene expression databases

ArrayExpressiP23906.
BgeeiP23906.
CleanExiMM_IRF2.
GenevestigatoriP23906.

Interactioni

Subunit structurei

Interacts with BRD7, IRF2BP1 and IRF2BP2. Interacts with CREBBP in growing cells; the interaction acetylates IRF2 and regulates IRF2-dependent H4 promoter activity By similarity.

Protein-protein interaction databases

BioGridi200785. 1 interaction.
IntActiP23906. 1 interaction.
MINTiMINT-4098768.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi8 – 1710
Beta strandi25 – 284
Turni29 – 324
Beta strandi33 – 375
Beta strandi43 – 475
Helixi48 – 514
Helixi53 – 619
Turni67 – 693
Helixi74 – 8714
Beta strandi91 – 933
Turni95 – 973
Beta strandi98 – 1003
Beta strandi106 – 1116

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1IRFNMR-A2-113[»]
1IRGNMR-A2-113[»]
2IRFX-ray2.20G/H/I/J/K/L1-113[»]
ProteinModelPortaliP23906.
SMRiP23906. Positions 5-113.

Miscellaneous databases

EvolutionaryTraceiP23906.

Family & Domainsi

Sequence similaritiesi

Belongs to the IRF family.

Phylogenomic databases

eggNOGiNOG41436.
HOGENOMiHOG000037937.
HOVERGENiHBG003455.
InParanoidiP23906.
KOiK10153.
OMAiSWPPFPD.
OrthoDBiEOG72ZCFD.
PhylomeDBiP23906.
TreeFamiTF328512.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR017431. Interferon_reg_fac-1/2.
IPR019817. Interferon_reg_fac_CS.
IPR001346. Interferon_reg_fact_DNA-bd_dom.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00605. IRF. 1 hit.
[Graphical view]
PIRSFiPIRSF038196. IFN_RF1/2. 1 hit.
PRINTSiPR00267. INTFRNREGFCT.
SMARTiSM00348. IRF. 1 hit.
[Graphical view]
PROSITEiPS00601. IRF_1. 1 hit.
PS51507. IRF_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P23906-1 [UniParc]FASTAAdd to Basket

« Hide

MPVERMRMRP WLEEQINSNT IPGLKWLNKE KKIFQIPWMH AARHGWDVEK    50
DAPLFRNWAI HTGKHQPGID KPDPKTWKAN FRCAMNSLPD IEEVKDRSIK 100
KGNNAFRVYR MLPLSERPSK KGKKPKTEKE ERVKHIKQEP VESSLGLSNG 150
VSGFSPEYAV LTSAIKNEVD STVNIIVVGQ SHLDSNIEDQ EIVTNPPDIC 200
QVVEVTTESD DQPVSMSELY PLQISPVSSY AESETTDSVA SDEENAEGRP 250
HWRKRSIEGK QYLSNMGTRN TYLLPSMATF VTSNKPDLQV TIKEDSCPMP 300
YNSSWPPFTD LPLPAPVTPT PSSSRPDRET RASVIKKTSD ITQARVKSC 349
Length:349
Mass (Da):39,453
Last modified:November 1, 1991 - v1
Checksum:i8738B082FB40FB11
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J03168 mRNA. Translation: AAA39333.1.
CCDSiCCDS22295.1.
RefSeqiNP_032417.3. NM_008391.4.
XP_006509351.1. XM_006509288.1.
XP_006509352.1. XM_006509289.1.
UniGeneiMm.1149.

Genome annotation databases

EnsembliENSMUST00000034041; ENSMUSP00000034041; ENSMUSG00000031627.
GeneIDi16363.
KEGGimmu:16363.
UCSCiuc009lqo.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J03168 mRNA. Translation: AAA39333.1 .
CCDSi CCDS22295.1.
RefSeqi NP_032417.3. NM_008391.4.
XP_006509351.1. XM_006509288.1.
XP_006509352.1. XM_006509289.1.
UniGenei Mm.1149.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1IRF NMR - A 2-113 [» ]
1IRG NMR - A 2-113 [» ]
2IRF X-ray 2.20 G/H/I/J/K/L 1-113 [» ]
ProteinModelPortali P23906.
SMRi P23906. Positions 5-113.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 200785. 1 interaction.
IntActi P23906. 1 interaction.
MINTi MINT-4098768.

PTM databases

PhosphoSitei P23906.

Proteomic databases

PaxDbi P23906.
PRIDEi P23906.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000034041 ; ENSMUSP00000034041 ; ENSMUSG00000031627 .
GeneIDi 16363.
KEGGi mmu:16363.
UCSCi uc009lqo.2. mouse.

Organism-specific databases

CTDi 3660.
MGIi MGI:96591. Irf2.

Phylogenomic databases

eggNOGi NOG41436.
HOGENOMi HOG000037937.
HOVERGENi HBG003455.
InParanoidi P23906.
KOi K10153.
OMAi SWPPFPD.
OrthoDBi EOG72ZCFD.
PhylomeDBi P23906.
TreeFami TF328512.

Enzyme and pathway databases

Reactomei REACT_198521. TRAF6 mediated IRF7 activation.
REACT_198649. Factors involved in megakaryocyte development and platelet production.
REACT_198660. Interferon gamma signaling.

Miscellaneous databases

EvolutionaryTracei P23906.
NextBioi 289470.
PROi P23906.
SOURCEi Search...

Gene expression databases

ArrayExpressi P23906.
Bgeei P23906.
CleanExi MM_IRF2.
Genevestigatori P23906.

Family and domain databases

Gene3Di 1.10.10.10. 1 hit.
InterProi IPR017431. Interferon_reg_fac-1/2.
IPR019817. Interferon_reg_fac_CS.
IPR001346. Interferon_reg_fact_DNA-bd_dom.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view ]
Pfami PF00605. IRF. 1 hit.
[Graphical view ]
PIRSFi PIRSF038196. IFN_RF1/2. 1 hit.
PRINTSi PR00267. INTFRNREGFCT.
SMARTi SM00348. IRF. 1 hit.
[Graphical view ]
PROSITEi PS00601. IRF_1. 1 hit.
PS51507. IRF_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Structurally similar but functionally distinct factors, IRF-1 and IRF-2, bind to the same regulatory elements of IFN and IFN-inducible genes."
    Harada H., Fujita T., Miyamoto M., Kimura Y., Maruyama M., Furia A., Miyata T., Taniguchi T.
    Cell 58:729-739(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. Lubec G., Sunyer B., Chen W.-Q.
    Submitted (JAN-2009) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 108-120, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: OF1.
    Tissue: Hippocampus.
  3. "Solution structure of the IRF-2 DNA-binding domain: a novel subgroup of the winged helix-turn-helix family."
    Furui J., Uegaki K., Yamazaki T., Shirakawa M., Swindells M.B., Harada H., Taniguchi T., Kyogoku Y.
    Structure 6:491-500(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR OF 2-113.
  4. "Crystal structure of an IRF-DNA complex reveals novel DNA recognition and cooperative binding to a tandem repeat of core sequences."
    Fujii Y., Shimizu T., Kusumoto M., Kyogoku Y., Taniguchi T., Hakoshima T.
    EMBO J. 18:5028-5041(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS) OF 1-113.

Entry informationi

Entry nameiIRF2_MOUSE
AccessioniPrimary (citable) accession number: P23906
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: November 1, 1991
Last modified: September 3, 2014
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi