Reviewed, UniProtKB/Swiss-Prot P23906 (IRF2_MOUSE)

Names and origin

Protein names

Recommended name:
    Interferon regulatory factor 2
      Short name=IRF-2

Gene names

Name:

Irf2

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	349 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Specifically binds to the upstream regulatory region of type I IFN and IFN-inducible MHC class I genes (the interferon consensus sequence (ICS)) and represses those genes. Also acts as an activator for several genes including H4 and IL7. Constitutively binds to the ISRE promoter to activate IL7. Involved in cell cycle regulation through binding the site II (HiNF-M) promoter region of H4 and activating transcription during cell growth. Antagonizes IRF1 transcriptional activation.
Subunit structure	Interacts with BRD7, IRF2BP1 and IRF2BP2. Interacts with CREBBP in growing cells; the interaction acetylates IRF2 and regulates IRF2-dependent H4 promoter activity By similarity.
Subcellular location	Nucleus.
Induction	By viruses and IFN.
Post-translational modification	Acetylated by CBP/ p300 during cell-growth. Acetylation on Lys-75 is required for stimulation of H4 promoter activity By similarity. The major sites of sumoylation are Lys-137 and Lys-293. Sumoylation by SUMO1 increases its transcriptional repressor activity on IRF1 and diminishes its ability to activate ISRE and H4 promoter By similarity.
Sequence similarities	Belongs to the IRF family. Contains 1 tryptophan pentad repeat DNA-binding domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Ligand	DNA-binding
Molecular function	Activator Repressor
PTM	Acetylation Isopeptide bond Ubl conjugation
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological process	regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	transcription factor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 349

349

Interferon regulatory factor 2

PRO_0000154550

Regions

DNA binding

7 – 109

103

Tryptophan pentad repeat

Amino acid modifications

Modified residue

75

1

N6-acetyllysine By similarity

Modified residue

78

1

N6-acetyllysine By similarity

Cross-link

137

Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO) By similarity

Cross-link

166

Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO) By similarity

Cross-link

293

Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO) By similarity

Secondary structure

1

.

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349

Helix Strand Turn

Details...

Helix

8 – 17

10

Beta strand

25 – 28

4

Turn

29 – 32

4

Beta strand

33 – 37

5

Helix

48 – 51

4

Helix

53 – 61

9

Turn

67 – 69

3

Helix

74 – 87

14

Beta strand

91 – 93

3

Turn

95 – 97

3

Beta strand

106 – 111

6

Sequences

	Sequence		Length	Mass (Da)
	P23906-1 [UniParc]. Last modified November 1, 1991. Version 1. Checksum: 8738B082FB40FB11	FASTA	349	39,453
	10 20 30 40 50 60 MPVERMRMRP WLEEQINSNT IPGLKWLNKE KKIFQIPWMH AARHGWDVEK DAPLFRNWAI 70 80 90 100 110 120 HTGKHQPGID KPDPKTWKAN FRCAMNSLPD IEEVKDRSIK KGNNAFRVYR MLPLSERPSK 130 140 150 160 170 180 KGKKPKTEKE ERVKHIKQEP VESSLGLSNG VSGFSPEYAV LTSAIKNEVD STVNIIVVGQ 190 200 210 220 230 240 SHLDSNIEDQ EIVTNPPDIC QVVEVTTESD DQPVSMSELY PLQISPVSSY AESETTDSVA 250 260 270 280 290 300 SDEENAEGRP HWRKRSIEGK QYLSNMGTRN TYLLPSMATF VTSNKPDLQV TIKEDSCPMP 310 320 330 340 YNSSWPPFTD LPLPAPVTPT PSSSRPDRET RASVIKKTSD ITQARVKSC

References

[1]	"Structurally similar but functionally distinct factors, IRF-1 and IRF-2, bind to the same regulatory elements of IFN and IFN-inducible genes." Harada H., Fujita T., Miyamoto M., Kimura Y., Maruyama M., Furia A., Miyata T., Taniguchi T. Cell 58:729-739(1989) [PubMed: 2475256] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	Lubec G., Sunyer B., Chen W.-Q. Submitted (JAN-2009) to UniProtKB Cited for: PROTEIN SEQUENCE OF 108-120, MASS SPECTROMETRY. Strain: OF1. Tissue: Hippocampus.
[3]	"Solution structure of the IRF-2 DNA-binding domain: a novel subgroup of the winged helix-turn-helix family." Furui J., Uegaki K., Yamazaki T., Shirakawa M., Swindells M.B., Harada H., Taniguchi T., Kyogoku Y. Structure 6:491-500(1998) [PubMed: 9562558] [Abstract] Cited for: STRUCTURE BY NMR OF 2-113.
[4]	"Crystal structure of an IRF-DNA complex reveals novel DNA recognition and cooperative binding to a tandem repeat of core sequences." Fujii Y., Shimizu T., Kusumoto M., Kyogoku Y., Taniguchi T., Hakoshima T. EMBO J. 18:5028-5041(1999) [PubMed: 10487755] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS) OF 1-113.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

J03168 mRNA. Translation: AAA39333.1.

IPI

IPI00138234.

RefSeq

NP_032417.3.

UniGene

Mm.1149

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1IRF	NMR	-	A	2-113	[»]
1IRG	NMR	-	A	2-113	[»]
2IRF	X-ray	2.20	G/H/I/J/K/L	1-113	[»]

ModBase

Search...

Protein-protein interaction databases

STRING

P23906.

PTM databases

PhosphoSite

P23906.

Proteomic databases

PRIDE

P23906.

Genome annotation databases

Ensembl

ENSMUST00000034041; ENSMUSP00000034041; ENSMUSG00000031627; Mus musculus. [Genome view]

GeneID

16363.

KEGG

mmu:16363.

Organism-specific databases

CTD

16363.

MGI

MGI:96591. Irf2.

Phylogenomic databases

HOGENOM

P23906.

HOVERGEN

P23906.

OMA

VTIKEES.

Gene expression databases

ArrayExpress

P23906.

Bgee

P23906.

CleanEx

MM_IRF2.

Genevestigator

P23906.

GermOnline

ENSMUSG00000031627. Mus musculus.

Family and domain databases

InterPro

IPR017431. Interferon_regulatory_fac-1/2.
IPR019817. Interferon_regulatory_fac_CS.
IPR001346. Interferon_regulatory_factor.
IPR011991. Wing_hlx_DNA_bd.
[Graphical view]

Gene3D

G3DSA:1.10.10.10. Wing_hlx_DNA_bd. 1 hit.

Pfam

PF00605. IRF. 1 hit.
[Graphical view]

PIRSF

PIRSF038196. IFN_RF1/2. 1 hit.

PRINTS

PR00267. INTFRNREGFCT.

ProDom

PD002355. IRF. 1 hit.
[Graphical view] [Entries sharing at least one domain]

SMART

SM00348. IRF. 1 hit.
[Graphical view]

PROSITE

PS00601. IRF. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

289470.

SOURCE

Search...

Entry information

Entry name

IRF2_MOUSE

Accession

Primary (citable) accession number: P23906

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1991
Last sequence update:	November 1, 1991
Last modified:	November 3, 2009
This is version 81 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)