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Protein

Beta-glucanase

Gene
N/A
Organism
Paenibacillus macerans (Bacillus macerans)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Hydrolysis of (1->4)-beta-D-glucosidic linkages in beta-D-glucans containing (1->3)- and (1->4)-bonds.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei128NucleophilePROSITE-ProRule annotation1
Active sitei132Proton donorPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Protein family/group databases

CAZyiGH16. Glycoside Hydrolase Family 16.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-glucanase (EC:3.2.1.73)
Alternative name(s):
1,3-1,4-beta-D-glucan 4-glucanohydrolase
Endo-beta-1,3-1,4 glucanase
Lichenase
OrganismiPaenibacillus macerans (Bacillus macerans)
Taxonomic identifieri44252 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesPaenibacillaceaePaenibacillus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi128E → D, N, A, L, P, R, H, C, S or Y: Loss of activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 23Sequence analysisAdd BLAST23
ChainiPRO_000001178924 – 237Beta-glucanaseAdd BLAST214

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi55 ↔ 841 Publication

Keywords - PTMi

Disulfide bond

Structurei

Secondary structure

1237
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi28 – 31Combined sources4
Beta strandi38 – 43Combined sources6
Beta strandi46 – 48Combined sources3
Beta strandi55 – 57Combined sources3
Helixi59 – 61Combined sources3
Beta strandi62 – 64Combined sources3
Beta strandi70 – 78Combined sources9
Beta strandi81 – 91Combined sources11
Beta strandi95 – 103Combined sources9
Beta strandi110 – 118Combined sources9
Helixi120 – 122Combined sources3
Beta strandi127 – 134Combined sources8
Helixi135 – 137Combined sources3
Beta strandi140 – 147Combined sources8
Beta strandi155 – 158Combined sources4
Helixi163 – 165Combined sources3
Beta strandi168 – 175Combined sources8
Beta strandi178 – 183Combined sources6
Beta strandi186 – 191Combined sources6
Beta strandi200 – 211Combined sources12
Helixi213 – 216Combined sources4
Beta strandi225 – 236Combined sources12

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1AJKX-ray1.80A/B107-237[»]
1AJOX-ray2.07A/B37-149[»]
1AXKX-ray2.10A/B82-237[»]
1BYHX-ray2.80A40-237[»]
1CPMX-ray2.00A82-237[»]
1CPNX-ray1.80A82-237[»]
1GLHX-ray2.00A37-237[»]
1MACX-ray2.30A/B26-237[»]
1U0AX-ray1.64A/B/C/D37-237[»]
2AYHX-ray1.60A37-237[»]
ProteinModelPortaliP23904.
SMRiP23904.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP23904.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini28 – 237GH16PROSITE-ProRule annotationAdd BLAST210

Sequence similaritiesi

Belongs to the glycosyl hydrolase 16 family.Curated
Contains 1 GH16 (glycosyl hydrolase family 16) domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
InterProiIPR008264. Beta_glucanase.
IPR013320. ConA-like_dom.
IPR000757. GH16.
IPR008263. GH16_AS.
[Graphical view]
PfamiPF00722. Glyco_hydro_16. 1 hit.
[Graphical view]
PRINTSiPR00737. GLHYDRLASE16.
SUPFAMiSSF49899. SSF49899. 1 hit.
PROSITEiPS01034. GH16_1. 1 hit.
PS51762. GH16_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P23904-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKKSCFTLV TTFAFSLIFS VSALAGSVFW EPLSYFNRST WEKADGYSNG
60 70 80 90 100
GVFNCTWRAN NVNFTNDGKL KLGLTSSAYN KFDCAEYRST NIYGYGLYEV
110 120 130 140 150
SMKPAKNTGI VSSFFTYTGP AHGTQWDEID IEFLGKDTTK VQFNYYTNGV
160 170 180 190 200
GGHEKVISLG FDASKGFHTY AFDWQPGYIK WYVDGVLKHT ATANIPSTPG
210 220 230
KIMMNLWNGT GVDDWLGSYN GANPLYAEYD WVKYTSN
Length:237
Mass (Da):26,589
Last modified:June 1, 1994 - v2
Checksum:i436EABCDFFC87781
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X55959 Genomic DNA. Translation: CAA39426.1.
PIRiS11927.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X55959 Genomic DNA. Translation: CAA39426.1.
PIRiS11927.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1AJKX-ray1.80A/B107-237[»]
1AJOX-ray2.07A/B37-149[»]
1AXKX-ray2.10A/B82-237[»]
1BYHX-ray2.80A40-237[»]
1CPMX-ray2.00A82-237[»]
1CPNX-ray1.80A82-237[»]
1GLHX-ray2.00A37-237[»]
1MACX-ray2.30A/B26-237[»]
1U0AX-ray1.64A/B/C/D37-237[»]
2AYHX-ray1.60A37-237[»]
ProteinModelPortaliP23904.
SMRiP23904.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGH16. Glycoside Hydrolase Family 16.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiP23904.

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
InterProiIPR008264. Beta_glucanase.
IPR013320. ConA-like_dom.
IPR000757. GH16.
IPR008263. GH16_AS.
[Graphical view]
PfamiPF00722. Glyco_hydro_16. 1 hit.
[Graphical view]
PRINTSiPR00737. GLHYDRLASE16.
SUPFAMiSSF49899. SSF49899. 1 hit.
PROSITEiPS01034. GH16_1. 1 hit.
PS51762. GH16_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGUB_PAEMA
AccessioniPrimary (citable) accession number: P23904
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: June 1, 1994
Last modified: November 2, 2016
This is version 98 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Beta-glucanases of Bacillus have a substrate range similar to lichenase of germinating barley.

Keywords - Technical termi

3D-structure

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.