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P23904

- GUB_PAEMA

UniProt

P23904 - GUB_PAEMA

Protein

Beta-glucanase

Gene
N/A
Organism
Paenibacillus macerans (Bacillus macerans)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 91 (01 Oct 2014)
      Sequence version 2 (01 Jun 1994)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Hydrolysis of (1->4)-beta-D-glucosidic linkages in beta-D-glucans containing (1->3)- and (1->4)-bonds.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei128 – 1281NucleophilePROSITE-ProRule annotation
    Active sitei132 – 1321Proton donorPROSITE-ProRule annotation

    GO - Molecular functioni

    1. licheninase activity Source: UniProtKB-EC

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Protein family/group databases

    CAZyiGH16. Glycoside Hydrolase Family 16.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-glucanase (EC:3.2.1.73)
    Alternative name(s):
    1,3-1,4-beta-D-glucan 4-glucanohydrolase
    Endo-beta-1,3-1,4 glucanase
    Lichenase
    OrganismiPaenibacillus macerans (Bacillus macerans)
    Taxonomic identifieri44252 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesPaenibacillaceaePaenibacillus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi128 – 1281E → D, N, A, L, P, R, H, C, S or Y: Loss of activity. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2323Sequence AnalysisAdd
    BLAST
    Chaini24 – 237214Beta-glucanasePRO_0000011789Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi55 ↔ 841 Publication

    Keywords - PTMi

    Disulfide bond

    Structurei

    Secondary structure

    1
    237
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi28 – 314
    Beta strandi38 – 436
    Beta strandi46 – 483
    Beta strandi55 – 573
    Helixi59 – 613
    Beta strandi62 – 643
    Beta strandi70 – 789
    Beta strandi81 – 9111
    Beta strandi95 – 1039
    Beta strandi110 – 1189
    Helixi120 – 1223
    Beta strandi127 – 1348
    Helixi135 – 1373
    Beta strandi140 – 1478
    Beta strandi155 – 1584
    Helixi163 – 1653
    Beta strandi168 – 1758
    Beta strandi178 – 1836
    Beta strandi186 – 1916
    Beta strandi200 – 21112
    Helixi213 – 2164
    Beta strandi225 – 23612

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1AJKX-ray1.80A/B107-237[»]
    1AJOX-ray2.07A/B37-149[»]
    1AXKX-ray2.10A/B82-237[»]
    1BYHX-ray2.80A40-237[»]
    1CPMX-ray2.00A82-237[»]
    1CPNX-ray1.80A82-237[»]
    1GLHX-ray2.00A37-237[»]
    1MACX-ray2.30A/B26-237[»]
    1U0AX-ray1.64A/B/C/D37-237[»]
    2AYHX-ray1.60A37-237[»]
    ProteinModelPortaliP23904.
    SMRiP23904. Positions 22-237.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP23904.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 16 family.Curated

    Keywords - Domaini

    Signal

    Family and domain databases

    Gene3Di2.60.120.200. 1 hit.
    InterProiIPR008264. Beta_glucanase.
    IPR008985. ConA-like_lec_gl_sf.
    IPR013320. ConA-like_subgrp.
    IPR000757. Glyco_hydro_16.
    IPR008263. Glycoside_hydrolase_16_AS.
    [Graphical view]
    PfamiPF00722. Glyco_hydro_16. 1 hit.
    [Graphical view]
    PRINTSiPR00737. GLHYDRLASE16.
    SUPFAMiSSF49899. SSF49899. 1 hit.
    PROSITEiPS01034. GLYCOSYL_HYDROL_F16. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P23904-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKKKSCFTLV TTFAFSLIFS VSALAGSVFW EPLSYFNRST WEKADGYSNG    50
    GVFNCTWRAN NVNFTNDGKL KLGLTSSAYN KFDCAEYRST NIYGYGLYEV 100
    SMKPAKNTGI VSSFFTYTGP AHGTQWDEID IEFLGKDTTK VQFNYYTNGV 150
    GGHEKVISLG FDASKGFHTY AFDWQPGYIK WYVDGVLKHT ATANIPSTPG 200
    KIMMNLWNGT GVDDWLGSYN GANPLYAEYD WVKYTSN 237
    Length:237
    Mass (Da):26,589
    Last modified:June 1, 1994 - v2
    Checksum:i436EABCDFFC87781
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X55959 Genomic DNA. Translation: CAA39426.1.
    PIRiS11927.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X55959 Genomic DNA. Translation: CAA39426.1 .
    PIRi S11927.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1AJK X-ray 1.80 A/B 107-237 [» ]
    1AJO X-ray 2.07 A/B 37-149 [» ]
    1AXK X-ray 2.10 A/B 82-237 [» ]
    1BYH X-ray 2.80 A 40-237 [» ]
    1CPM X-ray 2.00 A 82-237 [» ]
    1CPN X-ray 1.80 A 82-237 [» ]
    1GLH X-ray 2.00 A 37-237 [» ]
    1MAC X-ray 2.30 A/B 26-237 [» ]
    1U0A X-ray 1.64 A/B/C/D 37-237 [» ]
    2AYH X-ray 1.60 A 37-237 [» ]
    ProteinModelPortali P23904.
    SMRi P23904. Positions 22-237.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GH16. Glycoside Hydrolase Family 16.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Miscellaneous databases

    EvolutionaryTracei P23904.

    Family and domain databases

    Gene3Di 2.60.120.200. 1 hit.
    InterProi IPR008264. Beta_glucanase.
    IPR008985. ConA-like_lec_gl_sf.
    IPR013320. ConA-like_subgrp.
    IPR000757. Glyco_hydro_16.
    IPR008263. Glycoside_hydrolase_16_AS.
    [Graphical view ]
    Pfami PF00722. Glyco_hydro_16. 1 hit.
    [Graphical view ]
    PRINTSi PR00737. GLHYDRLASE16.
    SUPFAMi SSF49899. SSF49899. 1 hit.
    PROSITEi PS01034. GLYCOSYL_HYDROL_F16. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Structure of the beta-1,3-1,4-glucanase gene of Bacillus macerans: homologies to other beta-glucanases."
      Borriss R., Buettner K., Maentsaelae P.
      Mol. Gen. Genet. 222:278-283(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Identification of glutamic acid 105 at the active site of Bacillus amyloliquefaciens 1,3-1,4-beta-D-glucan 4-glucanohydrolase using epoxide-based inhibitors."
      Hoej P.B., Condron R., Traeger J.C., McAuliffe J.C., Stone B.A.
      J. Biol. Chem. 267:25059-25066(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACTIVE SITE.
    3. Olsen O.
      Thesis (1990), University of Aarhus, Denmark
      Cited for: MUTAGENESIS OF GLU-128.
    4. "Molecular and active-site structure of a Bacillus 1,3-1,4-beta-glucanase."
      Keitel T., Simon O., Borriss R., Heinemann U.
      Proc. Natl. Acad. Sci. U.S.A. 90:5287-5291(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS).
    5. "Crystal and molecular structure at 0.16-nm resolution of the hybrid Bacillus endo-1,3-1,4-beta-D-glucan 4-glucanohydrolase H(A16-M)."
      Hahn M., Keitel T., Heinemann U.
      Eur. J. Biochem. 232:849-858(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS).

    Entry informationi

    Entry nameiGUB_PAEMA
    AccessioniPrimary (citable) accession number: P23904
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1991
    Last sequence update: June 1, 1994
    Last modified: October 1, 2014
    This is version 91 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Beta-glucanases of Bacillus have a substrate range similar to lichenase of germinating barley.

    Keywords - Technical termi

    3D-structure

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3