P23903 (E13B_BACCI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glucan endo-1,3-beta-glucosidase A1
EC=3.2.1.39

Alternative name(s):
(1->3)-beta-glucan endohydrolase
(1->3)-beta-glucanase A1

Gene names

Name:

glcA

Organism

Bacillus circulans

Taxonomic identifier

1397 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	682 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Lysis of cellular walls containing beta-1,3-glucans. Implicated in the defense against fungal pathogens.
Catalytic activity	Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-beta-D-glucans.
Subcellular location	Secreted By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 16 family.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation
Cellular component	Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	glucan endo-1,3-beta-D-glucosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 38	38	Ref.1
	Chain	39 – 682	644	Glucan endo-1,3-beta-glucosidase A1		PRO_0000011796
Sites
	Active site	552	1	Nucleophile By similarity
	Active site	557	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	P23903 [UniParc]. Last modified November 1, 1991. Version 1. Checksum: 8C4F407E34D4ADD5	FASTA	682	75,466
	10 20 30 40 50 60 MKPSHFTEKR FMKKVLGLFL VVVMLASVGV LPTSKVQAAG TTVTSMEYFS PADGPVISKS 70 80 90 100 110 120 GVGKASYGFV MPKFNGGSAT WNDVYSDVGV NVKVGNNWVD IDQAGGYIYN QNWGHWSDGG 130 140 150 160 170 180 FNGYWFTLSA TTEIQLYSKA NGVKLEYQLV FQNINKTTIT AMNPTQGPQI TASFTGGAGF 190 200 210 220 230 240 TYPTFNNDSA VTYEAVADDL KVYVKPVNSS SWIDIDNNAA SGWIYDHNFG QFTDGGGGYW 250 260 270 280 290 300 FNVTESINVK LESKTSSANL VYTITFNEPT RNSYVITPYE GTTFTADANG SIGIPLPKID 310 320 330 340 350 360 GGAPIAKELG NFVYQINING QWVDLSNSSQ SKFAYSANGY NNMSDANQWG YWADYIYGLW 370 380 390 400 410 420 FQPIQENMQI RIGYPLNGQA GGNIGNNFVN YTFIGNPNAP RPDVSDQEDI SIGTPTDPAI 430 440 450 460 470 480 AGMNLIWQDE FNGTTLDTSK WNYETGYYLN NDPATWGWGN AELQHYTNST QNVYVQDGKL 490 500 510 520 530 540 NIKAMNDSKS FPQDPNRYAQ YSSGKINTKD KLSLKYGRVD FRAKLPTGDG VWPALWMLPK 550 560 570 580 590 600 DSVYGTWAAS GEIDVMEARG RLPGSVSGTI HFGGQWPVNQ SSGGDYHFPE GQTFANDYHV 610 620 630 640 650 660 YSVVWEEDNI KWYVDGKFFY KVTNQQWYST AAPNNPNAPF DEPFYLIMNL AVGGNFDGGR 670 680 TPNASDIPAT MQVDYVRVYK EQ

References

[1]	"Structure of the gene encoding beta-1,3-glucanase A1 of Bacillus circulans WL-12." Yahata N., Watanabe T., Nakamura Y., Yamamoto Y., Kamimiya S., Tanaka H. Gene 86:113-117(1990) [PubMed: 2311931] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 39-52. Strain: WL-12.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M34503 Genomic DNA. Translation: AAA22474.1.
PIR	JQ0420.
3D structure databases
ProteinModelPortal	P23903.
ModBase	Search...
Protein family/group databases
CAZy	GH16. Glycoside Hydrolase Family 16.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR008985. ConA-like_lec_gl. IPR013320. ConA-like_subgrp. IPR000757. Glyco_hydro_16. IPR008263. Glycoside_hydrolase_16_AS. [Graphical view]
Gene3D	G3DSA:2.60.120.200. ConA_like_subgrp. 1 hit.
Pfam	PF00722. Glyco_hydro_16. 1 hit. [Graphical view]
SUPFAM	SSF49899. ConA_like_lec_gl. 1 hit.
PROSITE	PS01034. GLYCOSYL_HYDROL_F16. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

E13B_BACCI

Accession

Primary (citable) accession number: P23903

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1991
Last sequence update:	November 1, 1991
Last modified:	September 21, 2011
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program