Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

3-oxoacyl-[acyl-carrier-protein] synthase I, chloroplastic

Gene

KAS12

Organism
Hordeum vulgare (Barley)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the condensation reaction of fatty acid synthesis by the addition to an acyl acceptor of two carbons from malonyl-ACP. Specific for elongation from C-10 to unsaturated C-16 and C-18 fatty acids.

Catalytic activityi

Acyl-[acyl-carrier-protein] + malonyl-[acyl-carrier-protein] = 3-oxoacyl-[acyl-carrier-protein] + CO2 + [acyl-carrier-protein].

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei213 – 2131PROSITE-ProRule annotation

GO - Molecular functioni

  1. 3-oxoacyl-[acyl-carrier-protein] synthase activity Source: UniProtKB-EC

GO - Biological processi

  1. fatty acid biosynthetic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism

Names & Taxonomyi

Protein namesi
Recommended name:
3-oxoacyl-[acyl-carrier-protein] synthase I, chloroplastic (EC:2.3.1.41)
Alternative name(s):
Beta-ketoacyl-ACP synthase I
Short name:
KAS I
Gene namesi
Name:KAS12
OrganismiHordeum vulgare (Barley)
Taxonomic identifieri4513 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladePooideaeTriticeaeHordeum

Organism-specific databases

GrameneiP23902.

Subcellular locationi

GO - Cellular componenti

  1. chloroplast Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 3535ChloroplastAdd
BLAST
Chaini36 – 4624273-oxoacyl-[acyl-carrier-protein] synthase I, chloroplasticPRO_0000000587Add
BLAST

Proteomic databases

PRIDEiP23902.

Expressioni

Gene expression databases

GenevestigatoriP23902.

Interactioni

Subunit structurei

Homodimer.

Structurei

3D structure databases

ProteinModelPortaliP23902.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the beta-ketoacyl-ACP synthases family.Curated

Keywords - Domaini

Transit peptide

Family and domain databases

Gene3Di3.40.47.10. 2 hits.
InterProiIPR017568. 3-oxoacyl-ACP_synth-2.
IPR018201. Ketoacyl_synth_AS.
IPR014031. Ketoacyl_synth_C.
IPR014030. Ketoacyl_synth_N.
IPR016039. Thiolase-like.
IPR016038. Thiolase-like_subgr.
[Graphical view]
PfamiPF00109. ketoacyl-synt. 1 hit.
PF02801. Ketoacyl-synt_C. 1 hit.
[Graphical view]
PIRSFiPIRSF000447. KAS_II. 1 hit.
SUPFAMiSSF53901. SSF53901. 2 hits.
TIGRFAMsiTIGR03150. fabF. 1 hit.
PROSITEiPS00606. B_KETOACYL_SYNTHASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P23902-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHAHAAHALG LRVPPPAFPR RRARPRRRPA AAVLATSAAP QRETDPRKRV
60 70 80 90 100
VITGMGLASV FGSDVDTFYD RLLAGESGVG PIDRFDASSF PTRFAGQIRG
110 120 130 140 150
FSSEGYIDGK NDRRLDDCIR YCILSGKKAL ESAGLGAGSD AHVKLDVGRA
160 170 180 190 200
GVLVGTGMGG LSVFSDGVQN LIEKGYRKIS PFFIPYAITN MGSALLAIDV
210 220 230 240 250
GFMGPNYSIS TACATSNYCF YAAANHIRRG EADIIVAGGT EAAIIPIGLG
260 270 280 290 300
GFVACRALSQ RNDDPITACR PWDKERDGFV MGEGAGVLVM ESLEHAMKRD
310 320 330 340 350
APIIAEYLGG AVNCDAYHMT DPRADGLGVS SCITMSLRDA GVAPEEVNYI
360 370 380 390 400
NAHATSTLAG DLAEVRAIKQ VFKNPSEIKI NSTKSMIGHC LGAAGGLEAI
410 420 430 440 450
ATIKSITTGW VHPTINQFNP EPEVDFDTVA NEKKQHEVNV GISNSFGFGG
460
HNSVVVFAPF KP
Length:462
Mass (Da):49,016
Last modified:October 31, 1991 - v1
Checksum:iBDFA3622263A7764
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M60410 mRNA. Translation: AAA32968.1.
PIRiA39356.
UniGeneiHv.115.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M60410 mRNA. Translation: AAA32968.1.
PIRiA39356.
UniGeneiHv.115.

3D structure databases

ProteinModelPortaliP23902.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiP23902.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

GrameneiP23902.

Gene expression databases

GenevestigatoriP23902.

Family and domain databases

Gene3Di3.40.47.10. 2 hits.
InterProiIPR017568. 3-oxoacyl-ACP_synth-2.
IPR018201. Ketoacyl_synth_AS.
IPR014031. Ketoacyl_synth_C.
IPR014030. Ketoacyl_synth_N.
IPR016039. Thiolase-like.
IPR016038. Thiolase-like_subgr.
[Graphical view]
PfamiPF00109. ketoacyl-synt. 1 hit.
PF02801. Ketoacyl-synt_C. 1 hit.
[Graphical view]
PIRSFiPIRSF000447. KAS_II. 1 hit.
SUPFAMiSSF53901. SSF53901. 2 hits.
TIGRFAMsiTIGR03150. fabF. 1 hit.
PROSITEiPS00606. B_KETOACYL_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Primary structure of a cerulenin-binding beta-ketoacyl-[acyl carrier protein] synthase from barley chloroplasts."
    Siggaard-Andersen M., Kauppinen S., von Wettstein-Knowles P.
    Proc. Natl. Acad. Sci. U.S.A. 88:4114-4118(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
    Strain: cv. Bonus.

Entry informationi

Entry nameiKASC1_HORVU
AccessioniPrimary (citable) accession number: P23902
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 1991
Last sequence update: October 31, 1991
Last modified: January 6, 2015
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.