P23901 (ALDR_HORVU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Aldose reductase Short name=AR EC=1.1.1.21 Alternative name(s): Aldehyde reductase
Organism	Hordeum vulgare (Barley)
Taxonomic identifier	4513 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › Liliopsida › commelinids › Poales › Poaceae › BEP clade › Pooideae › Triticeae › Hordeum

Protein attributes

Sequence length	320 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Alditol + NAD(P)+ = aldose + NAD(P)H.
Induction	By abscisic acid (ABA) and gibberellic acid (GA).
Sequence similarities	Belongs to the aldo/keto reductase family.

Ontologies

Keywords
Biological process	Stress response
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	3D-structure
Gene Ontology (GO)
Biological_process	response to stress Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	alditol:NADP+ 1-oxidoreductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 320

320

Aldose reductase

PRO_0000124628

Regions

Nucleotide binding

215 – 269

55

NADP By similarity

Sites

Active site

60

1

Proton donor By similarity

Binding site

121

1

Substrate By similarity

Site

88

1

Lowers pKa of active site Tyr By similarity

Secondary structure

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320

Helix Strand Turn

Details...

Beta strand

15 – 17

3

Beta strand

23 – 27

5

Helix

35 – 37

3

Helix

38 – 47

10

Beta strand

53 – 55

3

Helix

58 – 60

3

Helix

63 – 75

13

Helix

80 – 82

3

Beta strand

84 – 89

6

Helix

91 – 93

3

Helix

96 – 98

3

Helix

99 – 110

12

Beta strand

115 – 123

9

Helix

144 – 156

13

Beta strand

159 – 167

9

Helix

170 – 179

10

Beta strand

185 – 190

6

Helix

198 – 206

9

Beta strand

210 – 215

6

Turn

219 – 222

4

Helix

224 – 226

3

Helix

228 – 237

10

Helix

241 – 252

12

Beta strand

255 – 257

3

Helix

263 – 268

6

Helix

279 – 287

9

Helix

298 – 301

4

Turn

304 – 306

3

Helix

312 – 315

4

Turn

316 – 318

3

Sequences

	Sequence		Length	Mass (Da)
	P23901 [UniParc]. Last modified November 1, 1991. Version 1. Checksum: 5AA72C021E9A93C0	FASTA	320	35,807
	10 20 30 40 50 60 MASAKATMGQ GEQDHFVLKS GHAMPAVGLG TWRAGSDTAH SVRTAITEAG YRHVDTAAEY 70 80 90 100 110 120 GVEKEVGKGL KAAMEAGIDR KDLFVTSKIW CTNLAPERVR PALENTLKDL QLDYIDLYHI 130 140 150 160 170 180 HWPFRLKDGA HMPPEAGEVL EFDMEGVWKE MENLVKDGLV KDIGVCNYTV TKLNRLLRSA 190 200 210 220 230 240 KIPPAVCQME MHPGWKNDKI FEACKKHGIH VTAYSPLGSS EKNLAHDPVV EKVANKLNKT 250 260 270 280 290 300 PGQVLIKWAL QRGTSVIPKS SKDERIKENI QVFGWEIPEE DFKVLCSIKD EKRVLTGEEL 310 320 FVNKTHGPYR SAADVWDHEN

References

[1]	"An ABA and GA modulated gene expressed in the barley embryo encodes an aldose reductase related protein." Bartels D., Engelhardt K., Roncarati R., Schneider K., Rotter M., Salamini F. EMBO J. 10:1037-1043(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. Aura.
[2]	"Barley aldose reductase: structure, cofactor binding, and substrate recognition in the aldo/keto reductase 4C family." Olsen J.G., Pedersen L., Christensen C.L., Olsen O., Henriksen A. Proteins 71:1572-1581(2008) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.64 ANGSTROMS) OF 2-320.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X57526 Genomic DNA. Translation: CAA40747.1.

PIR

S15024.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2BGQ	X-ray	2.50	A	2-320	[»]
2BGS	X-ray	1.64	A	2-320	[»]
2VDG	X-ray	1.92	A	2-320	[»]

ProteinModelPortal

P23901.

SMR

P23901. Positions 13-320.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Organism-specific databases

Gramene

P23901.

Gene expression databases

Genevestigator

P23901.

Family and domain databases

Gene3D

3.20.20.100. 1 hit.

InterPro

IPR001395. Aldo/ket_red.
IPR018170. Aldo/ket_reductase_CS.
IPR020471. Aldo/keto_reductase_subgr.
IPR023210. NADP_OxRdtase_dom.
[Graphical view]

PANTHER

PTHR11732. PTHR11732. 1 hit.

Pfam

PF00248. Aldo_ket_red. 1 hit.
[Graphical view]

PIRSF

PIRSF000097. AKR. 1 hit.

PRINTS

PR00069. ALDKETRDTASE.

SUPFAM

SSF51430. Aldo/ket_red. 1 hit.

PROSITE

PS00798. ALDOKETO_REDUCTASE_1. 1 hit.
PS00062. ALDOKETO_REDUCTASE_2. 1 hit.
PS00063. ALDOKETO_REDUCTASE_3. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P23901.

Entry information

Entry name

ALDR_HORVU

Accession

Primary (citable) accession number: P23901

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1991
Last sequence update:	November 1, 1991
Last modified:	April 3, 2013
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program