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Protein

Hepatocyte nuclear factor 1-beta

Gene

Hnf1b

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcription factor, probably binds to the inverted palindrome 5'-GTTAATNATTAAC-3'.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi231 – 31181Homeobox; HNF1-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  • core promoter proximal region DNA binding Source: RGD
  • DNA binding Source: UniProtKB
  • protein complex binding Source: RGD
  • protein heterodimerization activity Source: RGD
  • protein homodimerization activity Source: UniProtKB
  • sequence-specific DNA binding Source: RGD
  • transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding Source: RGD
  • transcription factor activity, sequence-specific DNA binding Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Hepatocyte nuclear factor 1-beta
Short name:
HNF-1-beta
Short name:
HNF-1B
Alternative name(s):
Transcription factor 2
Short name:
TCF-2
Variant hepatic nuclear factor 1
Short name:
vHNF1
Gene namesi
Name:Hnf1b
Synonyms:Hnf-1b, Tcf2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 10

Organism-specific databases

RGDi3830. Hnf1b.

Subcellular locationi

GO - Cellular componenti

  • nucleoplasm Source: Ensembl
  • nucleus Source: UniProtKB
  • transcription factor complex Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 557557Hepatocyte nuclear factor 1-betaPRO_0000049124Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei49 – 491PhosphoserineCombined sources
Modified residuei52 – 521PhosphoserineBy similarity
Modified residuei75 – 751PhosphoserineCombined sources
Modified residuei80 – 801PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

PTM databases

iPTMnetiP23899.
PhosphoSiteiP23899.

Expressioni

Tissue specificityi

Liver, kidney and intestine.

Gene expression databases

ExpressionAtlasiP23899. baseline and differential.
GenevisibleiP23899. RN.

Interactioni

Subunit structurei

Binds DNA as a dimer. Can form homodimer or heterodimer with HNF1-alpha.

GO - Molecular functioni

  • protein complex binding Source: RGD
  • protein heterodimerization activity Source: RGD
  • protein homodimerization activity Source: UniProtKB

Structurei

3D structure databases

ProteinModelPortaliP23899.
SMRiP23899. Positions 1-31, 91-321.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 3131DimerizationBy similarityAdd
BLAST

Sequence similaritiesi

Belongs to the HNF1 homeobox family.Curated
Contains 1 homeobox DNA-binding domain.PROSITE-ProRule annotation

Keywords - Domaini

Homeobox

Phylogenomic databases

GeneTreeiENSGT00730000110937.
HOVERGENiHBG005980.
InParanoidiP23899.
KOiK08034.
PhylomeDBiP23899.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
1.10.260.40. 1 hit.
InterProiIPR006899. HNF-1_N.
IPR023219. HNF1_dimer_dom.
IPR006897. HNF1b_C.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR010982. Lambda_DNA-bd_dom.
[Graphical view]
PfamiPF04814. HNF-1_N. 1 hit.
PF04812. HNF-1B_C. 1 hit.
PF00046. Homeobox. 1 hit.
[Graphical view]
SMARTiSM00389. HOX. 1 hit.
[Graphical view]
SUPFAMiSSF100957. SSF100957. 1 hit.
SSF46689. SSF46689. 1 hit.
SSF47413. SSF47413. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P23899-1) [UniParc]FASTAAdd to basket

Also known as: VHNF1-A

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVSKLTSLQQ ELLSALLSSG VTKEVLIQAL EELLPSPNFG VKLETLPLSP
60 70 80 90 100
GSGADLDTKP VFHTLTNGHA KGRLSGDEGS EDGDDYDTPP ILKELQALNT
110 120 130 140 150
EEAAEQRAEV DRMLSEDPWR AAKMIKGYMQ QHNIPQREVV DVTGLNQSHL
160 170 180 190 200
SQHLNKGTPM KTQKRAALYT WYVRKQREIL RQFNQTVQSS GNMTDKSSQD
210 220 230 240 250
QLLFLFPEFS QQNQGPGQSE DACSEPTNKK MRRNRFKWGP ASQQILYQAY
260 270 280 290 300
DRQKNPSKEE REALVEECNR AECLQRGVSP SKAHGLGSNL VTEVRVYNWF
310 320 330 340 350
ANRRKEEAFR QKLAMDAYSS NQTHNLNPLL THGSPHHQPS SSPPNKLSGV
360 370 380 390 400
RYSQPGNNEV TSSSTISHHG NSAMVTSQSV LQQVSPASLD PGHSLLSPDS
410 420 430 440 450
KMISVSGGGL PPVSTLTNIH SLSHHNPQQS QNLIMTPLSG VMAIAQSLNT
460 470 480 490 500
SQAQGVPVIN SVASSLAALQ PVQFSQQLHS PHQQPLMQQS PGSHMAQQPF
510 520 530 540 550
MAAVTQLQNS HMYAHKQEPP QYSHTSRFPS AMVVTDTSSI NTLTSMSSSK

QCPLQAW
Length:557
Mass (Da):61,371
Last modified:November 1, 1991 - v1
Checksum:i7729E78C458B6DBC
GO
Isoform 2 (identifier: P23899-2) [UniParc]FASTAAdd to basket

Also known as: VHNF1-B

The sequence of this isoform differs from the canonical sequence as follows:
     183-208: Missing.

Show »
Length:531
Mass (Da):58,426
Checksum:i3818C87AD2F184EB
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei183 – 20826Missing in isoform 2. 1 PublicationVSP_002257Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X56546 mRNA. Translation: CAA39886.1.
BC081826 mRNA. Translation: AAH81826.1.
PIRiS15342.
RefSeqiNP_037235.1. NM_013103.2. [P23899-1]
UniGeneiRn.11342.

Genome annotation databases

EnsembliENSRNOT00000047427; ENSRNOP00000046025; ENSRNOG00000002598. [P23899-1]
GeneIDi25640.
KEGGirno:25640.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X56546 mRNA. Translation: CAA39886.1.
BC081826 mRNA. Translation: AAH81826.1.
PIRiS15342.
RefSeqiNP_037235.1. NM_013103.2. [P23899-1]
UniGeneiRn.11342.

3D structure databases

ProteinModelPortaliP23899.
SMRiP23899. Positions 1-31, 91-321.
ModBaseiSearch...
MobiDBiSearch...

PTM databases

iPTMnetiP23899.
PhosphoSiteiP23899.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000047427; ENSRNOP00000046025; ENSRNOG00000002598. [P23899-1]
GeneIDi25640.
KEGGirno:25640.

Organism-specific databases

CTDi6928.
RGDi3830. Hnf1b.

Phylogenomic databases

GeneTreeiENSGT00730000110937.
HOVERGENiHBG005980.
InParanoidiP23899.
KOiK08034.
PhylomeDBiP23899.

Miscellaneous databases

PROiP23899.

Gene expression databases

ExpressionAtlasiP23899. baseline and differential.
GenevisibleiP23899. RN.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
1.10.260.40. 1 hit.
InterProiIPR006899. HNF-1_N.
IPR023219. HNF1_dimer_dom.
IPR006897. HNF1b_C.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR010982. Lambda_DNA-bd_dom.
[Graphical view]
PfamiPF04814. HNF-1_N. 1 hit.
PF04812. HNF-1B_C. 1 hit.
PF00046. Homeobox. 1 hit.
[Graphical view]
SMARTiSM00389. HOX. 1 hit.
[Graphical view]
SUPFAMiSSF100957. SSF100957. 1 hit.
SSF46689. SSF46689. 1 hit.
SSF47413. SSF47413. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "vHNF1 is a homeoprotein that activates transcription and forms heterodimers with HNF1."
    Rey-Campos J., Chouard T., Yaniv M., Cereghini S.
    EMBO J. 10:1445-1457(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
    Tissue: Liver.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Kidney.
  3. "Quantitative phosphoproteomics of vasopressin-sensitive renal cells: regulation of aquaporin-2 phosphorylation at two sites."
    Hoffert J.D., Pisitkun T., Wang G., Shen R.-F., Knepper M.A.
    Proc. Natl. Acad. Sci. U.S.A. 103:7159-7164(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  4. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
    Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
    Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-49; SER-75 AND SER-80, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiHNF1B_RAT
AccessioniPrimary (citable) accession number: P23899
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: November 1, 1991
Last modified: June 8, 2016
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.