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P23897

- GUC2C_RAT

UniProt

P23897 - GUC2C_RAT

Protein

Heat-stable enterotoxin receptor

Gene

Gucy2c

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 131 (01 Oct 2014)
      Sequence version 2 (01 Feb 1996)
      Previous versions | rss
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    Functioni

    Receptor for the E.coli heat-stable enterotoxin (E.coli enterotoxin markedly stimulates the accumulation of cGMP in mammalian cells expressing GC-C). Also activated by the endogenous peptide guanylin.

    Catalytic activityi

    GTP = 3',5'-cyclic GMP + diphosphate.

    GO - Molecular functioni

    1. ATP binding Source: InterPro
    2. GTP binding Source: UniProtKB-KW
    3. guanylate cyclase activity Source: RGD
    4. protein kinase activity Source: InterPro
    5. toxic substance binding Source: RGD

    GO - Biological processi

    1. intracellular signal transduction Source: InterPro
    2. regulation of cell proliferation Source: Ensembl
    3. response to toxic substance Source: Ensembl

    Keywords - Molecular functioni

    Lyase, Receptor

    Keywords - Biological processi

    cGMP biosynthesis

    Keywords - Ligandi

    GTP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Heat-stable enterotoxin receptor (EC:4.6.1.2)
    Short name:
    STA receptor
    Alternative name(s):
    Guanylyl cyclase C
    Short name:
    GC-C
    Intestinal guanylate cyclase
    Gene namesi
    Name:Gucy2c
    Synonyms:Guc2c
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 4

    Organism-specific databases

    RGDi2771. Gucy2c.

    Subcellular locationi

    Cell membrane; Single-pass type I membrane protein. Endoplasmic reticulum membrane By similarity; Single-pass type I membrane protein
    Note: The 145 kDa plasma membrane form of GC-C contains sialic acid and galactose residues, while a differencially glycosylated 130 Kda form is a high mannose form that is resident in the endoplasmic reticulum and may serve as the precursor for the cell surface form.By similarity

    GO - Cellular componenti

    1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW
    3. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Endoplasmic reticulum, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2222Sequence AnalysisAdd
    BLAST
    Chaini23 – 10721050Heat-stable enterotoxin receptorPRO_0000012378Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi31 – 311N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi74 – 741N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi78 – 781N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi187 – 1871N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi194 – 1941N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi306 – 3061N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi401 – 4011N-linked (GlcNAc...)Sequence Analysis

    Post-translational modificationi

    Glycosylation at Asn-74 and/or Asn-78 is required for interaction with VIP36 while glycosylation at Asn-401 modulates ligand-mediated GC-C activation.By similarity

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PaxDbiP23897.
    PRIDEiP23897.

    PTM databases

    PhosphoSiteiP23897.

    Expressioni

    Tissue specificityi

    Intestine and possibly at low levels in additional tissues.

    Gene expression databases

    GenevestigatoriP23897.

    Interactioni

    Subunit structurei

    Homotrimer. Interacts via its C-terminal region with PDZK2. Interacts with the lectin chaperone VIP36 By similarity.By similarity

    Protein-protein interaction databases

    MINTiMINT-4996544.
    STRINGi10116.ENSRNOP00000012212.

    Structurei

    3D structure databases

    ProteinModelPortaliP23897.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini23 – 429407ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini454 – 1072619CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei430 – 45324HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini488 – 748261Protein kinasePROSITE-ProRule annotationAdd
    BLAST
    Domaini823 – 953131Guanylate cyclasePROSITE-ProRule annotationAdd
    BLAST

    Domaini

    The protein kinase domain is predicted to be catalytically inactive.

    Sequence similaritiesi

    Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.PROSITE-ProRule annotation
    Contains 1 guanylate cyclase domain.PROSITE-ProRule annotation
    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiKOG1023.
    GeneTreeiENSGT00730000110520.
    HOGENOMiHOG000112833.
    HOVERGENiHBG106967.
    InParanoidiP23897.
    KOiK12320.
    OMAiAYHDAVL.
    OrthoDBiEOG72VH58.
    PhylomeDBiP23897.

    Family and domain databases

    Gene3Di3.30.70.1230. 1 hit.
    InterProiIPR001054. A/G_cyclase.
    IPR018297. A/G_cyclase_CS.
    IPR001828. ANF_lig-bd_rcpt.
    IPR011009. Kinase-like_dom.
    IPR028082. Peripla_BP_I.
    IPR000719. Prot_kinase_dom.
    IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
    [Graphical view]
    PfamiPF01094. ANF_receptor. 1 hit.
    PF00211. Guanylate_cyc. 1 hit.
    PF07714. Pkinase_Tyr. 1 hit.
    [Graphical view]
    SMARTiSM00044. CYCc. 1 hit.
    [Graphical view]
    SUPFAMiSSF53822. SSF53822. 1 hit.
    SSF55073. SSF55073. 1 hit.
    SSF56112. SSF56112. 1 hit.
    PROSITEiPS00452. GUANYLATE_CYCLASE_1. 1 hit.
    PS50125. GUANYLATE_CYCLASE_2. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P23897-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTSLLGLAVR LLLFQPTLMF WASQVRQKCH NGTYEISVLM MDNSAYKEPL     50
    QNLRDAVEEG LDIVRKRLRE AELNVTVNAT FIYSDGLIHK SGDCRSSTCE 100
    GLDLLREITR DRKMGCVLMG PSCTYSTFQM YLDTELNYPM ISAGSFGLSC 150
    DYKETLTRIL PPARKLMYFL VDFWKVNNAP FKTFSWNSSY VYKNGSEPED 200
    CFWYLNALEA GVSYFSEVLS FKDVLRRSEQ FQEILMGRNR KSNVIVMCGT 250
    PETFYNVKGD LKVADDTVVI LVDLFSNHYF EDDTRAPEYM DNVLVLTLPP 300
    EKFIANASVS GRFPSERSDF SLAYLEGTLL FGHMLQTFLE NGESVTTPKF 350
    ARAFRNLTFQ GLEGPVTLDD SGDIDNIMCL LYVSLDTRKY KVLMAYDTHK 400
    NQTIPVATSP NFIWKNHRLP NDVPGLGPQI LMIAVFTLTG IVVVLLLIAL 450
    LVLRKYRRDH ELRQKKWSHI PSENIFPLET NETNHVSLKI DDDRRRDTIQ 500
    RVRQCKYDKK KVILKDLKHC DGNFSEKQKI ELNKLLQSDY YNLTKFYGTV 550
    KLDTRIFGVV EYCERGSLRE VLNDTISYPD GTFMDWEFKI SVLNDIAKGM 600
    SYLHSSKIEV HGRLKSTNCV VDSRMVVKIT DFGCNSILPP KKDLWTAPEH 650
    LRQATISQKG DVYSFSIIAQ EIILRKETFY TLSCRDQNEK IFRVENSYGT 700
    KPFRPDLFLE TADEKELEVY LLVKSCWEED PEKRPDFKKI ESTLAKIFGL 750
    FHDQKNESYM DTLIRRLQLY SRNLEHLVEE RTQLYKAERD RADHLNFMLL 800
    PRLVVKSLKE KGIVEPELYE EVTIYFSDIV GFTTICKYST PMEVVDMLND 850
    IYKSFDQIVD HHDVYKVETI GDAYVVASGL PMRNGNRHAV DISKMALDIL 900
    SFMGTFELEH LPGLPVWIRI GVHSGPCAAG VVGIKMPRYC LFGDTVNTAS 950
    RMESTGLPLR IHMSSSTIAI LRRTDCQFLY EVRGETYLKG RGTETTYWLT 1000
    GMKDQEYNLP TPPTVENQQR LQTEFSDMIV SALQKRQASG VKSRRPTRVA 1050
    SYKKGFLEYM QLNNSDHDST YF 1072
    Length:1,072
    Mass (Da):123,467
    Last modified:February 1, 1996 - v2
    Checksum:iEBA8656B2948C211
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M55636 mRNA. Translation: AAA41201.1.
    PIRiA36292. OYRTHX.
    RefSeqiNP_037302.1. NM_013170.1.
    UniGeneiRn.10931.

    Genome annotation databases

    EnsembliENSRNOT00000012212; ENSRNOP00000012212; ENSRNOG00000009031.
    GeneIDi25711.
    KEGGirno:25711.
    UCSCiRGD:2771. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M55636 mRNA. Translation: AAA41201.1 .
    PIRi A36292. OYRTHX.
    RefSeqi NP_037302.1. NM_013170.1.
    UniGenei Rn.10931.

    3D structure databases

    ProteinModelPortali P23897.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    MINTi MINT-4996544.
    STRINGi 10116.ENSRNOP00000012212.

    PTM databases

    PhosphoSitei P23897.

    Proteomic databases

    PaxDbi P23897.
    PRIDEi P23897.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000012212 ; ENSRNOP00000012212 ; ENSRNOG00000009031 .
    GeneIDi 25711.
    KEGGi rno:25711.
    UCSCi RGD:2771. rat.

    Organism-specific databases

    CTDi 2984.
    RGDi 2771. Gucy2c.

    Phylogenomic databases

    eggNOGi KOG1023.
    GeneTreei ENSGT00730000110520.
    HOGENOMi HOG000112833.
    HOVERGENi HBG106967.
    InParanoidi P23897.
    KOi K12320.
    OMAi AYHDAVL.
    OrthoDBi EOG72VH58.
    PhylomeDBi P23897.

    Miscellaneous databases

    NextBioi 607775.

    Gene expression databases

    Genevestigatori P23897.

    Family and domain databases

    Gene3Di 3.30.70.1230. 1 hit.
    InterProi IPR001054. A/G_cyclase.
    IPR018297. A/G_cyclase_CS.
    IPR001828. ANF_lig-bd_rcpt.
    IPR011009. Kinase-like_dom.
    IPR028082. Peripla_BP_I.
    IPR000719. Prot_kinase_dom.
    IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
    [Graphical view ]
    Pfami PF01094. ANF_receptor. 1 hit.
    PF00211. Guanylate_cyc. 1 hit.
    PF07714. Pkinase_Tyr. 1 hit.
    [Graphical view ]
    SMARTi SM00044. CYCc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53822. SSF53822. 1 hit.
    SSF55073. SSF55073. 1 hit.
    SSF56112. SSF56112. 1 hit.
    PROSITEi PS00452. GUANYLATE_CYCLASE_1. 1 hit.
    PS50125. GUANYLATE_CYCLASE_2. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Guanylyl cyclase is a heat-stable enterotoxin receptor."
      Schulz S., Green C.K., Yuen P.S.T., Garbers D.L.
      Cell 63:941-948(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Sprague-Dawley.
      Tissue: Small intestine.

    Entry informationi

    Entry nameiGUC2C_RAT
    AccessioniPrimary (citable) accession number: P23897
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1991
    Last sequence update: February 1, 1996
    Last modified: October 1, 2014
    This is version 131 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3