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Protein

Heat-stable enterotoxin receptor

Gene

Gucy2c

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Receptor for the E.coli heat-stable enterotoxin (E.coli enterotoxin markedly stimulates the accumulation of cGMP in mammalian cells expressing GC-C). Also activated by the endogenous peptide guanylin.

Catalytic activityi

GTP = 3',5'-cyclic GMP + diphosphate.

GO - Molecular functioni

  • ATP binding Source: InterPro
  • GTP binding Source: UniProtKB-KW
  • guanylate cyclase activity Source: RGD
  • protein kinase activity Source: InterPro
  • toxic substance binding Source: RGD

GO - Biological processi

Keywordsi

Molecular functionLyase, Receptor
Biological processcGMP biosynthesis
LigandGTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-RNO-8935690 Digestion

Names & Taxonomyi

Protein namesi
Recommended name:
Heat-stable enterotoxin receptor (EC:4.6.1.2)
Short name:
STA receptor
Alternative name(s):
Guanylyl cyclase C
Short name:
GC-C
Intestinal guanylate cyclase
Gene namesi
Name:Gucy2c
Synonyms:Guc2c
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 4

Organism-specific databases

RGDi2771 Gucy2c

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini23 – 429ExtracellularSequence analysisAdd BLAST407
Transmembranei430 – 453HelicalSequence analysisAdd BLAST24
Topological domaini454 – 1072CytoplasmicSequence analysisAdd BLAST619

Keywords - Cellular componenti

Cell membrane, Endoplasmic reticulum, Membrane

Pathology & Biotechi

Chemistry databases

GuidetoPHARMACOLOGYi1750

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 22Sequence analysisAdd BLAST22
ChainiPRO_000001237823 – 1072Heat-stable enterotoxin receptorAdd BLAST1050

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi31N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi74N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi78N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi187N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi194N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi306N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi401N-linked (GlcNAc...) asparagineSequence analysis1

Post-translational modificationi

Glycosylation at Asn-74 and/or Asn-78 is required for interaction with VIP36 while glycosylation at Asn-401 modulates ligand-mediated GC-C activation.By similarity

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiP23897
PRIDEiP23897

PTM databases

PhosphoSitePlusiP23897

Expressioni

Tissue specificityi

Intestine and possibly at low levels in additional tissues.

Gene expression databases

BgeeiENSRNOG00000009031
GenevisibleiP23897 RN

Interactioni

Subunit structurei

Homotrimer. Interacts via its C-terminal region with PDZK2. Interacts with the lectin chaperone VIP36 (By similarity).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000012212

Structurei

3D structure databases

ProteinModelPortaliP23897
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini488 – 748Protein kinasePROSITE-ProRule annotationAdd BLAST261
Domaini823 – 953Guanylate cyclasePROSITE-ProRule annotationAdd BLAST131

Domaini

The protein kinase domain is predicted to be catalytically inactive.

Sequence similaritiesi

Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.PROSITE-ProRule annotation

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1023 Eukaryota
COG2114 LUCA
GeneTreeiENSGT00760000118959
HOGENOMiHOG000112833
HOVERGENiHBG106967
InParanoidiP23897
KOiK12320
OMAiKYSTPME
OrthoDBiEOG091G01F8
PhylomeDBiP23897

Family and domain databases

Gene3Di3.30.70.1230, 1 hit
InterProiView protein in InterPro
IPR001054 A/G_cyclase
IPR018297 A/G_cyclase_CS
IPR011009 Kinase-like_dom_sf
IPR029787 Nucleotide_cyclase
IPR028082 Peripla_BP_I
IPR000719 Prot_kinase_dom
IPR001245 Ser-Thr/Tyr_kinase_cat_dom
PfamiView protein in Pfam
PF00211 Guanylate_cyc, 1 hit
PF07714 Pkinase_Tyr, 1 hit
SMARTiView protein in SMART
SM00044 CYCc, 1 hit
SUPFAMiSSF53822 SSF53822, 1 hit
SSF55073 SSF55073, 1 hit
SSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS00452 GUANYLATE_CYCLASE_1, 1 hit
PS50125 GUANYLATE_CYCLASE_2, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P23897-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTSLLGLAVR LLLFQPTLMF WASQVRQKCH NGTYEISVLM MDNSAYKEPL
60 70 80 90 100
QNLRDAVEEG LDIVRKRLRE AELNVTVNAT FIYSDGLIHK SGDCRSSTCE
110 120 130 140 150
GLDLLREITR DRKMGCVLMG PSCTYSTFQM YLDTELNYPM ISAGSFGLSC
160 170 180 190 200
DYKETLTRIL PPARKLMYFL VDFWKVNNAP FKTFSWNSSY VYKNGSEPED
210 220 230 240 250
CFWYLNALEA GVSYFSEVLS FKDVLRRSEQ FQEILMGRNR KSNVIVMCGT
260 270 280 290 300
PETFYNVKGD LKVADDTVVI LVDLFSNHYF EDDTRAPEYM DNVLVLTLPP
310 320 330 340 350
EKFIANASVS GRFPSERSDF SLAYLEGTLL FGHMLQTFLE NGESVTTPKF
360 370 380 390 400
ARAFRNLTFQ GLEGPVTLDD SGDIDNIMCL LYVSLDTRKY KVLMAYDTHK
410 420 430 440 450
NQTIPVATSP NFIWKNHRLP NDVPGLGPQI LMIAVFTLTG IVVVLLLIAL
460 470 480 490 500
LVLRKYRRDH ELRQKKWSHI PSENIFPLET NETNHVSLKI DDDRRRDTIQ
510 520 530 540 550
RVRQCKYDKK KVILKDLKHC DGNFSEKQKI ELNKLLQSDY YNLTKFYGTV
560 570 580 590 600
KLDTRIFGVV EYCERGSLRE VLNDTISYPD GTFMDWEFKI SVLNDIAKGM
610 620 630 640 650
SYLHSSKIEV HGRLKSTNCV VDSRMVVKIT DFGCNSILPP KKDLWTAPEH
660 670 680 690 700
LRQATISQKG DVYSFSIIAQ EIILRKETFY TLSCRDQNEK IFRVENSYGT
710 720 730 740 750
KPFRPDLFLE TADEKELEVY LLVKSCWEED PEKRPDFKKI ESTLAKIFGL
760 770 780 790 800
FHDQKNESYM DTLIRRLQLY SRNLEHLVEE RTQLYKAERD RADHLNFMLL
810 820 830 840 850
PRLVVKSLKE KGIVEPELYE EVTIYFSDIV GFTTICKYST PMEVVDMLND
860 870 880 890 900
IYKSFDQIVD HHDVYKVETI GDAYVVASGL PMRNGNRHAV DISKMALDIL
910 920 930 940 950
SFMGTFELEH LPGLPVWIRI GVHSGPCAAG VVGIKMPRYC LFGDTVNTAS
960 970 980 990 1000
RMESTGLPLR IHMSSSTIAI LRRTDCQFLY EVRGETYLKG RGTETTYWLT
1010 1020 1030 1040 1050
GMKDQEYNLP TPPTVENQQR LQTEFSDMIV SALQKRQASG VKSRRPTRVA
1060 1070
SYKKGFLEYM QLNNSDHDST YF
Length:1,072
Mass (Da):123,467
Last modified:February 1, 1996 - v2
Checksum:iEBA8656B2948C211
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M55636 mRNA Translation: AAA41201.1
PIRiA36292 OYRTHX
RefSeqiNP_037302.1, NM_013170.1
UniGeneiRn.10931

Genome annotation databases

EnsembliENSRNOT00000012212; ENSRNOP00000012212; ENSRNOG00000009031
GeneIDi25711
KEGGirno:25711
UCSCiRGD:2771 rat

Similar proteinsi

Entry informationi

Entry nameiGUC2C_RAT
AccessioniPrimary (citable) accession number: P23897
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: February 1, 1996
Last modified: May 23, 2018
This is version 152 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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