Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P23897 (GUC2C_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 130. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Heat-stable enterotoxin receptor

Short name=STA receptor
EC=4.6.1.2
Alternative name(s):
Guanylyl cyclase C
Short name=GC-C
Intestinal guanylate cyclase
Gene names
Name:Gucy2c
Synonyms:Guc2c
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length1072 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Receptor for the E.coli heat-stable enterotoxin (E.coli enterotoxin markedly stimulates the accumulation of cGMP in mammalian cells expressing GC-C). Also activated by the endogenous peptide guanylin.

Catalytic activity

GTP = 3',5'-cyclic GMP + diphosphate.

Subunit structure

Homotrimer. Interacts via its C-terminal region with PDZK2. Interacts with the lectin chaperone VIP36 By similarity.

Subcellular location

Cell membrane; Single-pass type I membrane protein. Endoplasmic reticulum membrane By similarity; Single-pass type I membrane protein. Note: The 145 kDa plasma membrane form of GC-C contains sialic acid and galactose residues, while a differencially glycosylated 130 Kda form is a high mannose form that is resident in the endoplasmic reticulum and may serve as the precursor for the cell surface form By similarity.

Tissue specificity

Intestine and possibly at low levels in additional tissues.

Domain

The protein kinase domain is predicted to be catalytically inactive.

Post-translational modification

Glycosylation at Asn-74 and/or Asn-78 is required for interaction with VIP36 while glycosylation at Asn-401 modulates ligand-mediated GC-C activation By similarity.

Sequence similarities

Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.

Contains 1 guanylate cyclase domain.

Contains 1 protein kinase domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2222 Potential
Chain23 – 10721050Heat-stable enterotoxin receptor
PRO_0000012378

Regions

Topological domain23 – 429407Extracellular Potential
Transmembrane430 – 45324Helical; Potential
Topological domain454 – 1072619Cytoplasmic Potential
Domain488 – 748261Protein kinase
Domain823 – 953131Guanylate cyclase

Amino acid modifications

Glycosylation311N-linked (GlcNAc...) Potential
Glycosylation741N-linked (GlcNAc...) Potential
Glycosylation781N-linked (GlcNAc...) Potential
Glycosylation1871N-linked (GlcNAc...) Potential
Glycosylation1941N-linked (GlcNAc...) Potential
Glycosylation3061N-linked (GlcNAc...) Potential
Glycosylation4011N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
P23897 [UniParc].

Last modified February 1, 1996. Version 2.
Checksum: EBA8656B2948C211

FASTA1,072123,467
        10         20         30         40         50         60 
MTSLLGLAVR LLLFQPTLMF WASQVRQKCH NGTYEISVLM MDNSAYKEPL QNLRDAVEEG 

        70         80         90        100        110        120 
LDIVRKRLRE AELNVTVNAT FIYSDGLIHK SGDCRSSTCE GLDLLREITR DRKMGCVLMG 

       130        140        150        160        170        180 
PSCTYSTFQM YLDTELNYPM ISAGSFGLSC DYKETLTRIL PPARKLMYFL VDFWKVNNAP 

       190        200        210        220        230        240 
FKTFSWNSSY VYKNGSEPED CFWYLNALEA GVSYFSEVLS FKDVLRRSEQ FQEILMGRNR 

       250        260        270        280        290        300 
KSNVIVMCGT PETFYNVKGD LKVADDTVVI LVDLFSNHYF EDDTRAPEYM DNVLVLTLPP 

       310        320        330        340        350        360 
EKFIANASVS GRFPSERSDF SLAYLEGTLL FGHMLQTFLE NGESVTTPKF ARAFRNLTFQ 

       370        380        390        400        410        420 
GLEGPVTLDD SGDIDNIMCL LYVSLDTRKY KVLMAYDTHK NQTIPVATSP NFIWKNHRLP 

       430        440        450        460        470        480 
NDVPGLGPQI LMIAVFTLTG IVVVLLLIAL LVLRKYRRDH ELRQKKWSHI PSENIFPLET 

       490        500        510        520        530        540 
NETNHVSLKI DDDRRRDTIQ RVRQCKYDKK KVILKDLKHC DGNFSEKQKI ELNKLLQSDY 

       550        560        570        580        590        600 
YNLTKFYGTV KLDTRIFGVV EYCERGSLRE VLNDTISYPD GTFMDWEFKI SVLNDIAKGM 

       610        620        630        640        650        660 
SYLHSSKIEV HGRLKSTNCV VDSRMVVKIT DFGCNSILPP KKDLWTAPEH LRQATISQKG 

       670        680        690        700        710        720 
DVYSFSIIAQ EIILRKETFY TLSCRDQNEK IFRVENSYGT KPFRPDLFLE TADEKELEVY 

       730        740        750        760        770        780 
LLVKSCWEED PEKRPDFKKI ESTLAKIFGL FHDQKNESYM DTLIRRLQLY SRNLEHLVEE 

       790        800        810        820        830        840 
RTQLYKAERD RADHLNFMLL PRLVVKSLKE KGIVEPELYE EVTIYFSDIV GFTTICKYST 

       850        860        870        880        890        900 
PMEVVDMLND IYKSFDQIVD HHDVYKVETI GDAYVVASGL PMRNGNRHAV DISKMALDIL 

       910        920        930        940        950        960 
SFMGTFELEH LPGLPVWIRI GVHSGPCAAG VVGIKMPRYC LFGDTVNTAS RMESTGLPLR 

       970        980        990       1000       1010       1020 
IHMSSSTIAI LRRTDCQFLY EVRGETYLKG RGTETTYWLT GMKDQEYNLP TPPTVENQQR 

      1030       1040       1050       1060       1070 
LQTEFSDMIV SALQKRQASG VKSRRPTRVA SYKKGFLEYM QLNNSDHDST YF 

« Hide

References

[1]"Guanylyl cyclase is a heat-stable enterotoxin receptor."
Schulz S., Green C.K., Yuen P.S.T., Garbers D.L.
Cell 63:941-948(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Sprague-Dawley.
Tissue: Small intestine.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M55636 mRNA. Translation: AAA41201.1.
PIROYRTHX. A36292.
RefSeqNP_037302.1. NM_013170.1.
UniGeneRn.10931.

3D structure databases

ProteinModelPortalP23897.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

MINTMINT-4996544.
STRING10116.ENSRNOP00000012212.

PTM databases

PhosphoSiteP23897.

Proteomic databases

PaxDbP23897.
PRIDEP23897.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000012212; ENSRNOP00000012212; ENSRNOG00000009031.
GeneID25711.
KEGGrno:25711.
UCSCRGD:2771. rat.

Organism-specific databases

CTD2984.
RGD2771. Gucy2c.

Phylogenomic databases

eggNOGKOG1023.
GeneTreeENSGT00730000110520.
HOGENOMHOG000112833.
HOVERGENHBG106967.
InParanoidP23897.
KOK12320.
OMAAYHDAVL.
OrthoDBEOG72VH58.
PhylomeDBP23897.

Gene expression databases

GenevestigatorP23897.

Family and domain databases

Gene3D3.30.70.1230. 1 hit.
InterProIPR001054. A/G_cyclase.
IPR018297. A/G_cyclase_CS.
IPR001828. ANF_lig-bd_rcpt.
IPR011009. Kinase-like_dom.
IPR028082. Peripla_BP_I.
IPR000719. Prot_kinase_dom.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
[Graphical view]
PfamPF01094. ANF_receptor. 1 hit.
PF00211. Guanylate_cyc. 1 hit.
PF07714. Pkinase_Tyr. 1 hit.
[Graphical view]
SMARTSM00044. CYCc. 1 hit.
[Graphical view]
SUPFAMSSF53822. SSF53822. 1 hit.
SSF55073. SSF55073. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEPS00452. GUANYLATE_CYCLASE_1. 1 hit.
PS50125. GUANYLATE_CYCLASE_2. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio607775.

Entry information

Entry nameGUC2C_RAT
AccessionPrimary (citable) accession number: P23897
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: February 1, 1996
Last modified: April 16, 2014
This is version 130 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families