##gff-version 3
P23895	UniProtKB	Chain	1	110	.	.	.	ID=PRO_0000108074;Note=Multidrug transporter EmrE	
P23895	UniProtKB	Transmembrane	4	21	.	.	.	Note=Helical%3B Name%3D1;Ontology_term=ECO:0000305;evidence=ECO:0000305|PubMed:17005200;Dbxref=PMID:17005200	
P23895	UniProtKB	Transmembrane	34	52	.	.	.	Note=Helical%3B Name%3D2;Ontology_term=ECO:0000305;evidence=ECO:0000305|PubMed:17005200;Dbxref=PMID:17005200	
P23895	UniProtKB	Transmembrane	58	80	.	.	.	Note=Helical%3B Name%3D3;Ontology_term=ECO:0000305;evidence=ECO:0000305|PubMed:17005200;Dbxref=PMID:17005200	
P23895	UniProtKB	Transmembrane	87	104	.	.	.	Note=Helical%3B Name%3D4;Ontology_term=ECO:0000305;evidence=ECO:0000305|PubMed:17005200;Dbxref=PMID:17005200	
P23895	UniProtKB	Site	4	4	.	.	.	Note=Required for proper coupling between the substrate transport and the proton gradient	
P23895	UniProtKB	Site	14	14	.	.	.	Note=Essential for translocation and for substrate and proton binding;Ontology_term=ECO:0000305,ECO:0000305;evidence=ECO:0000305|PubMed:12590142,ECO:0000305|PubMed:29114048;Dbxref=PMID:12590142,PMID:29114048	
P23895	UniProtKB	Site	40	40	.	.	.	Note=Involved in substrate binding	
P23895	UniProtKB	Site	60	60	.	.	.	Note=Involved in substrate binding	
P23895	UniProtKB	Site	63	63	.	.	.	Note=Involved in substrate binding	
P23895	UniProtKB	Site	110	110	.	.	.	Note=Important for activity;Ontology_term=ECO:0000305;evidence=ECO:0000305|PubMed:30287687;Dbxref=PMID:30287687	
P23895	UniProtKB	Mutagenesis	4	4	.	.	.	Note=Still binds substrate. No transport activity in the presence of a proton gradient%2C but still transports substrate in the absence of a proton gradient. Resistance to toxicants is abolished. Y->C;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:16672221;Dbxref=PMID:16672221	
P23895	UniProtKB	Mutagenesis	4	4	.	.	.	Note=No effect on resistance to toxicants. Y->F%2CW;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:16672221;Dbxref=PMID:16672221	
P23895	UniProtKB	Mutagenesis	6	6	.	.	.	Note=No effect on resistance to toxicants. Y->C%2CF%2CL;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:16672221;Dbxref=PMID:16672221	
P23895	UniProtKB	Mutagenesis	7	7	.	.	.	Note=No substrate binding. Resistance to toxicants is abolished. L->C;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:12590142;Dbxref=PMID:12590142	
P23895	UniProtKB	Mutagenesis	10	10	.	.	.	Note=Still binds substrate%2C with lower affinity. Resistance to toxicants is abolished. A->C;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:12590142;Dbxref=PMID:12590142	
P23895	UniProtKB	Mutagenesis	11	11	.	.	.	Note=Still binds substrate%2C with lower affinity. Resistance to toxicants is abolished. I->C;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:12590142;Dbxref=PMID:12590142	
P23895	UniProtKB	Mutagenesis	14	14	.	.	.	Note=No substrate binding. No transport activity. Resistance to toxicants is abolished. E->C;Ontology_term=ECO:0000269,ECO:0000269;evidence=ECO:0000269|PubMed:10681497,ECO:0000269|PubMed:12590142;Dbxref=PMID:10681497,PMID:12590142	
P23895	UniProtKB	Mutagenesis	14	14	.	.	.	Note=Still binds substrate. No transport activity in the presence of a proton gradient%2C but still transports substrate in the absence of a proton gradient. Resistance to toxicants is abolished. E->D;Ontology_term=ECO:0000269,ECO:0000269;evidence=ECO:0000269|PubMed:10681497,ECO:0000269|PubMed:12590142;Dbxref=PMID:10681497,PMID:12590142	
P23895	UniProtKB	Mutagenesis	17	17	.	.	.	Note=No substrate binding. Resistance to toxicants is abolished. G->C;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:12590142;Dbxref=PMID:12590142	
P23895	UniProtKB	Mutagenesis	18	18	.	.	.	Note=Still binds substrate%2C with lower affinity. Resistance to toxicants is abolished. T->C;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:12590142;Dbxref=PMID:12590142	
P23895	UniProtKB	Mutagenesis	40	40	.	.	.	Note=Modifies substrate specificity. Y->C%2CF%2CL%2CM%2CS%2CT%2CV;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:16672221;Dbxref=PMID:16672221	
P23895	UniProtKB	Mutagenesis	53	53	.	.	.	Note=No effect on resistance to toxicants. Y->C;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:16672221;Dbxref=PMID:16672221	
P23895	UniProtKB	Mutagenesis	60	60	.	.	.	Note=Still binds substrate%2C with lower affinity. Resistance to toxicants is abolished. Y->C%2CF;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:16672221;Dbxref=PMID:16672221	
P23895	UniProtKB	Mutagenesis	63	63	.	.	.	Note=No transport activity. Resistance to toxicants is abolished. W->C%2CY;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:15882076;Dbxref=PMID:15882076	
P23895	UniProtKB	Mutagenesis	63	63	.	.	.	Note=Still binds substrate%2C with two-fold reduction in substrate affinity. Resistance to toxicants is abolished. W->F;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:15882076;Dbxref=PMID:15882076	
P23895	UniProtKB	Mutagenesis	67	67	.	.	.	Note=Major destabilizing effect on the dimer form. G->W;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:23920359;Dbxref=PMID:23920359	
P23895	UniProtKB	Helix	3	23	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:7MH6	
P23895	UniProtKB	Beta strand	24	27	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:7MH6	
P23895	UniProtKB	Helix	30	50	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:7MH6	
P23895	UniProtKB	Turn	51	53	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:7MH6	
P23895	UniProtKB	Helix	56	78	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:7MH6	
P23895	UniProtKB	Helix	88	99	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:7MH6