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Protein

Protease HtpX

Gene

htpX

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Membrane-localized protease able to endoproteolytically degrade overproduced SecY but not YccA, another membrane protein. It seems to cleave SecY at specific cytoplasmic sites. Does not require ATP. Its natural substrate has not been identified. Probably plays a role in the quality control of integral membrane proteins.1 Publication

Cofactori

Zn2+1 PublicationNote: Binds 1 zinc ion per subunit.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi139Zinc; catalyticBy similarity1
Active sitei140By similarity1
Metal bindingi143Zinc; catalyticBy similarity1
Metal bindingi222Zinc; catalyticBy similarity1

GO - Molecular functioni

  • metalloendopeptidase activity Source: EcoCyc
  • zinc ion binding Source: EcoCyc

GO - Biological processi

  • proteolysis Source: EcoCyc
  • response to temperature stimulus Source: EcoCyc

Keywordsi

Molecular functionHydrolase, Metalloprotease, Protease
Biological processStress response
LigandMetal-binding, Zinc

Enzyme and pathway databases

BioCyciEcoCyc:EG10462-MONOMER

Protein family/group databases

MEROPSiM48.002
TCDBi9.B.1.1.6 the integral membrane caax protease (caax protease) family

Names & Taxonomyi

Protein namesi
Recommended name:
Protease HtpX (EC:3.4.24.-)
Alternative name(s):
Heat shock protein HtpX
Gene namesi
Name:htpX
Ordered Locus Names:b1829, JW1818
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10462 htpX

Subcellular locationi

  • Cell inner membrane 1 Publication; Multi-pass membrane protein 1 Publication
  • Note: Bioinformatics programs predict 4 transmembrane helices, however PhoA and Bla fusions as well as other experiments only confirm the first 2.1 Publication

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 3CytoplasmicSequence analysis3
Transmembranei4 – 24HelicalSequence analysisAdd BLAST21
Topological domaini25 – 33Periplasmic9
Transmembranei34 – 54HelicalSequence analysisAdd BLAST21
Topological domaini55 – 293CytoplasmicAdd BLAST239

GO - Cellular componenti

  • integral component of membrane Source: EcoCyc
  • plasma membrane Source: EcoCyc

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

No visible phenotype, however one of HtpX or FtsH is essential for cell viability.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi105N → D: Does not complement a double ftsH/htpX disruption mutant. 1 Publication1
Mutagenesisi107F → A: Does not complement a double ftsH/htpX disruption mutant. 1 Publication1
Mutagenesisi139H → F: Does not complement a double ftsH/htpX disruption mutant. No self-degradation. 1 Publication1
Mutagenesisi140E → Q: Does not complement a double ftsH/htpX disruption mutant. 1 Publication1
Mutagenesisi222E → Q: Does not complement a double ftsH/htpX disruption mutant. 1 Publication1
Mutagenesisi226D → N: Does not complement a double ftsH/htpX disruption mutant. 1 Publication1
Mutagenesisi276H → F: Does not complement a double ftsH/htpX disruption mutant. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001388601 – 293Protease HtpXAdd BLAST293

Post-translational modificationi

Undergoes self-cleavage. This may not be physiological.

Keywords - PTMi

Autocatalytic cleavage

Proteomic databases

PaxDbiP23894
PRIDEiP23894

Expressioni

Inductioni

By temperature upshift (by the sigma-32 heat shock transcription factor). Also under control of CpxR.2 Publications

Interactioni

Protein-protein interaction databases

BioGridi4260355, 36 interactors
STRINGi316385.ECDH10B_1968

Structurei

3D structure databases

ProteinModelPortaliP23894
SMRiP23894
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M48B family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105D0M Bacteria
COG0501 LUCA
HOGENOMiHOG000227302
InParanoidiP23894
KOiK03799
OMAiAVCCTEG
PhylomeDBiP23894

Family and domain databases

HAMAPiMF_00188 Pept_M48_protease_HtpX, 1 hit
InterProiView protein in InterPro
IPR022919 Pept_M48_protease_HtpX
IPR001915 Peptidase_M48
PfamiView protein in Pfam
PF01435 Peptidase_M48, 1 hit

Sequencei

Sequence statusi: Complete.

P23894-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMRIALFLLT NLAVMVVFGL VLSLTGIQSS SVQGLMIMAL LFGFGGSFVS
60 70 80 90 100
LLMSKWMALR SVGGEVIEQP RNERERWLVN TVATQARQAG IAMPQVAIYH
110 120 130 140 150
APDINAFATG ARRDASLVAV STGLLQNMSP DEAEAVIAHE ISHIANGDMV
160 170 180 190 200
TMTLIQGVVN TFVIFISRIL AQLAAGFMGG NRDEGEESNG NPLIYFAVAT
210 220 230 240 250
VLELVFGILA SIITMWFSRH REFHADAGSA KLVGREKMIA ALQRLKTSYE
260 270 280 290
PQEATSMMAL CINGKSKSLS ELFMTHPPLD KRIEALRTGE YLK
Length:293
Mass (Da):31,923
Last modified:November 1, 1991 - v1
Checksum:i13253716D92DD5BF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M58470 Genomic DNA Translation: AAA62779.1
U00096 Genomic DNA Translation: AAC74899.1
AP009048 Genomic DNA Translation: BAA15637.1
PIRiA43659
RefSeqiNP_416343.1, NC_000913.3
WP_000984520.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC74899; AAC74899; b1829
BAA15637; BAA15637; BAA15637
GeneIDi946076
KEGGiecj:JW1818
eco:b1829
PATRICifig|1411691.4.peg.421

Similar proteinsi

Entry informationi

Entry nameiHTPX_ECOLI
AccessioniPrimary (citable) accession number: P23894
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: November 1, 1991
Last modified: April 25, 2018
This is version 147 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health