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P23893

- GSA_ECOLI

UniProt

P23893 - GSA_ECOLI

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Protein
Glutamate-1-semialdehyde 2,1-aminomutase
Gene
hemL, gsa, popC, b0154, JW0150
Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

(S)-4-amino-5-oxopentanoate = 5-aminolevulinate.UniRule annotation

Cofactori

Pyridoxal phosphate.

Pathwayi

GO - Molecular functioni

  1. glutamate-1-semialdehyde 2,1-aminomutase activity Source: EcoCyc
  2. identical protein binding Source: EcoCyc
  3. protein binding Source: IntAct
  4. pyridoxal phosphate binding Source: InterPro
  5. transaminase activity Source: InterPro

GO - Biological processi

  1. protoporphyrinogen IX biosynthetic process Source: EcoCyc
  2. tetrapyrrole biosynthetic process Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Porphyrin biosynthesis

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

BioCyciEcoCyc:GSAAMINOTRANS-MONOMER.
ECOL316407:JW0150-MONOMER.
MetaCyc:GSAAMINOTRANS-MONOMER.
UniPathwayiUPA00251; UER00317.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate-1-semialdehyde 2,1-aminomutase (EC:5.4.3.8)
Short name:
GSA
Alternative name(s):
Glutamate-1-semialdehyde aminotransferase
Short name:
GSA-AT
Gene namesi
Name:hemL
Synonyms:gsa, popC
Ordered Locus Names:b0154, JW0150
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG10432. hemL.

Subcellular locationi

Cytoplasm Reviewed prediction UniRule annotation

GO - Cellular componenti

  1. cytosol Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi265 – 2651K → R: 2% of wild-type activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 426426Glutamate-1-semialdehyde 2,1-aminomutaseUniRule annotation
PRO_0000120408Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei265 – 2651N6-(pyridoxal phosphate)lysine Inferred

Proteomic databases

PaxDbiP23893.
PRIDEiP23893.

Expressioni

Inductioni

Induced by low extracellular levels of magnesium via the PhoQ/PhoP two-component regulatory system.1 Publication

Gene expression databases

GenevestigatoriP23893.

Interactioni

Subunit structurei

Homodimer.

Binary interactionsi

WithEntry#Exp.IntActNotes
glcCP0ACL54EBI-909193,EBI-1115389

Protein-protein interaction databases

DIPiDIP-9886N.
IntActiP23893. 2 interactions.
STRINGi511145.b0154.

Structurei

3D structure databases

ProteinModelPortaliP23893.
SMRiP23893. Positions 1-426.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0001.
HOGENOMiHOG000020210.
KOiK01845.
OMAiRAIKPYP.
OrthoDBiEOG6QVRHN.
PhylomeDBiP23893.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 2 hits.
HAMAPiMF_00375. HemL_aminotrans_3.
InterProiIPR004639. 4pyrrol_synth_GluAld_NH2Trfase.
IPR005814. Aminotrans_3.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PANTHERiPTHR11986. PTHR11986. 1 hit.
PfamiPF00202. Aminotran_3. 1 hit.
[Graphical view]
PIRSFiPIRSF000521. Transaminase_4ab_Lys_Orn. 1 hit.
SUPFAMiSSF53383. SSF53383. 1 hit.
TIGRFAMsiTIGR00713. hemL. 1 hit.
PROSITEiPS00600. AA_TRANSFER_CLASS_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P23893-1 [UniParc]FASTAAdd to Basket

« Hide

MSKSENLYSA ARELIPGGVN SPVRAFTGVG GTPLFIEKAD GAYLYDVDGK    50
AYIDYVGSWG PMVLGHNHPA IRNAVIEAAE RGLSFGAPTE MEVKMAQLVT 100
ELVPTMDMVR MVNSGTEATM SAIRLARGFT GRDKIIKFEG CYHGHADCLL 150
VKAGSGALTL GQPNSPGVPA DFAKYTLTCT YNDLASVRAA FEQYPQEIAC 200
IIVEPVAGNM NCVPPLPEFL PGLRALCDEF GALLIIDEVM TGFRVALAGA 250
QDYYGVVPDL TCLGKIIGGG MPVGAFGGRR DVMDALAPTG PVYQAGTLSG 300
NPIAMAAGFA CLNEVAQPGV HETLDELTTR LAEGLLEAAE EAGIPLVVNH 350
VGGMFGIFFT DAESVTCYQD VMACDVERFK RFFHMMLDEG VYLAPSAFEA 400
GFMSVAHSME DINNTIDAAR RVFAKL 426
Length:426
Mass (Da):45,366
Last modified:November 1, 1997 - v2
Checksum:iBED817E100468CF2
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti2 – 21S → R1 Publication
Sequence conflicti2 – 21S → R1 Publication
Sequence conflicti9 – 91S → Q1 Publication
Sequence conflicti9 – 91S → Q1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X53696 Genomic DNA. Translation: CAA37734.1.
U00096 Genomic DNA. Translation: AAC73265.1.
AP009048 Genomic DNA. Translation: BAB96731.2.
U70214 Genomic DNA. Translation: AAB08584.1.
PIRiB64739.
RefSeqiNP_414696.1. NC_000913.3.
YP_488457.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC73265; AAC73265; b0154.
BAB96731; BAB96731; BAB96731.
GeneIDi12931839.
946892.
KEGGiecj:Y75_p0151.
eco:b0154.
PATRICi32115417. VBIEscCol129921_0160.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X53696 Genomic DNA. Translation: CAA37734.1 .
U00096 Genomic DNA. Translation: AAC73265.1 .
AP009048 Genomic DNA. Translation: BAB96731.2 .
U70214 Genomic DNA. Translation: AAB08584.1 .
PIRi B64739.
RefSeqi NP_414696.1. NC_000913.3.
YP_488457.1. NC_007779.1.

3D structure databases

ProteinModelPortali P23893.
SMRi P23893. Positions 1-426.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-9886N.
IntActi P23893. 2 interactions.
STRINGi 511145.b0154.

Proteomic databases

PaxDbi P23893.
PRIDEi P23893.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC73265 ; AAC73265 ; b0154 .
BAB96731 ; BAB96731 ; BAB96731 .
GeneIDi 12931839.
946892.
KEGGi ecj:Y75_p0151.
eco:b0154.
PATRICi 32115417. VBIEscCol129921_0160.

Organism-specific databases

EchoBASEi EB0427.
EcoGenei EG10432. hemL.

Phylogenomic databases

eggNOGi COG0001.
HOGENOMi HOG000020210.
KOi K01845.
OMAi RAIKPYP.
OrthoDBi EOG6QVRHN.
PhylomeDBi P23893.

Enzyme and pathway databases

UniPathwayi UPA00251 ; UER00317 .
BioCyci EcoCyc:GSAAMINOTRANS-MONOMER.
ECOL316407:JW0150-MONOMER.
MetaCyc:GSAAMINOTRANS-MONOMER.

Miscellaneous databases

PROi P23893.

Gene expression databases

Genevestigatori P23893.

Family and domain databases

Gene3Di 3.40.640.10. 1 hit.
3.90.1150.10. 2 hits.
HAMAPi MF_00375. HemL_aminotrans_3.
InterProi IPR004639. 4pyrrol_synth_GluAld_NH2Trfase.
IPR005814. Aminotrans_3.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view ]
PANTHERi PTHR11986. PTHR11986. 1 hit.
Pfami PF00202. Aminotran_3. 1 hit.
[Graphical view ]
PIRSFi PIRSF000521. Transaminase_4ab_Lys_Orn. 1 hit.
SUPFAMi SSF53383. SSF53383. 1 hit.
TIGRFAMsi TIGR00713. hemL. 1 hit.
PROSITEi PS00600. AA_TRANSFER_CLASS_3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Structural genes of glutamate 1-semialdehyde aminotransferase for porphyrin synthesis in a cyanobacterium and Escherichia coli."
    Grimm B., Bull A., Breu V.
    Mol. Gen. Genet. 225:1-10(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Systematic sequencing of the Escherichia coli genome: analysis of the 2.4-4.1 min (110,917-193,643 bp) region."
    Fujita N., Mori H., Yura T., Ishihama A.
    Nucleic Acids Res. 22:1637-1639(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. "Sequence of minutes 4-25 of Escherichia coli."
    Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.
    Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  5. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], SEQUENCE REVISION TO 2 AND 9.
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  6. "The Escherichia coli hemL gene encodes glutamate 1-semialdehyde aminotransferase."
    Ilag L.L., Jahn D., Eggertsson G., Soell D.
    J. Bacteriol. 173:3408-3413(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  7. "Activity and spectroscopic properties of the Escherichia coli glutamate 1-semialdehyde aminotransferase and the putative active site mutant K265R."
    Ilag L.L., Jahn D.
    Biochemistry 31:7143-7151(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF LYS-265.
  8. "Identification and molecular characterization of the Mg2+ stimulon of Escherichia coli."
    Minagawa S., Ogasawara H., Kato A., Yamamoto K., Eguchi Y., Oshima T., Mori H., Ishihama A., Utsumi R.
    J. Bacteriol. 185:3696-3702(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
    Strain: K12.

Entry informationi

Entry nameiGSA_ECOLI
AccessioniPrimary (citable) accession number: P23893
Secondary accession number(s): P78277
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: November 1, 1997
Last modified: June 11, 2014
This is version 138 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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