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Protein

Glutamate-1-semialdehyde 2,1-aminomutase

Gene

hemL

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic activityi

(S)-4-amino-5-oxopentanoate = 5-aminolevulinate.

Cofactori

Pathwayi: protoporphyrin-IX biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes 5-aminolevulinate from L-glutamyl-tRNA(Glu).
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Glutamyl-tRNA reductase (hemA)
  2. Glutamate-1-semialdehyde 2,1-aminomutase (hemL)
This subpathway is part of the pathway protoporphyrin-IX biosynthesis, which is itself part of Porphyrin-containing compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-aminolevulinate from L-glutamyl-tRNA(Glu), the pathway protoporphyrin-IX biosynthesis and in Porphyrin-containing compound metabolism.

GO - Molecular functioni

  • glutamate-1-semialdehyde 2,1-aminomutase activity Source: EcoCyc
  • identical protein binding Source: EcoCyc
  • pyridoxal phosphate binding Source: InterPro
  • transaminase activity Source: InterPro

GO - Biological processi

  • protoporphyrinogen IX biosynthetic process Source: EcoCyc
  • tetrapyrrole biosynthetic process Source: EcoCyc

Keywordsi

Molecular functionIsomerase
Biological processPorphyrin biosynthesis
LigandPyridoxal phosphate

Enzyme and pathway databases

BioCyciEcoCyc:GSAAMINOTRANS-MONOMER
MetaCyc:GSAAMINOTRANS-MONOMER
UniPathwayiUPA00251; UER00317

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate-1-semialdehyde 2,1-aminomutase (EC:5.4.3.8)
Short name:
GSA
Alternative name(s):
Glutamate-1-semialdehyde aminotransferase
Short name:
GSA-AT
Gene namesi
Name:hemL
Synonyms:gsa, popC
Ordered Locus Names:b0154, JW0150
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10432 hemL

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: UniProtKB

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi265K → R: 2% of wild-type activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001204081 – 426Glutamate-1-semialdehyde 2,1-aminomutaseAdd BLAST426

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei265N6-(pyridoxal phosphate)lysineCurated1

Proteomic databases

EPDiP23893
PaxDbiP23893
PRIDEiP23893

Expressioni

Inductioni

Induced by low extracellular levels of magnesium via the PhoQ/PhoP two-component regulatory system.1 Publication

Interactioni

Subunit structurei

Homodimer.

Binary interactionsi

WithEntry#Exp.IntActNotes
glcCP0ACL54EBI-909193,EBI-1115389

GO - Molecular functioni

  • identical protein binding Source: EcoCyc

Protein-protein interaction databases

BioGridi426083331 interactors.
DIPiDIP-9886N
IntActiP23893 2 interactors.
STRINGi316385.ECDH10B_0134

Structurei

3D structure databases

ProteinModelPortaliP23893
SMRiP23893
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105CDM Bacteria
COG0001 LUCA
HOGENOMiHOG000020210
InParanoidiP23893
KOiK01845
OMAiWGPLIFG
PhylomeDBiP23893

Family and domain databases

CDDicd00610 OAT_like, 1 hit
Gene3Di3.40.640.101 hit
3.90.1150.102 hits
HAMAPiMF_00375 HemL_aminotrans_3, 1 hit
InterProiView protein in InterPro
IPR004639 4pyrrol_synth_GluAld_NH2Trfase
IPR005814 Aminotrans_3
IPR015424 PyrdxlP-dep_Trfase
IPR015422 PyrdxlP-dep_Trfase_dom1
IPR015421 PyrdxlP-dep_Trfase_major
PfamiView protein in Pfam
PF00202 Aminotran_3, 1 hit
SUPFAMiSSF53383 SSF53383, 1 hit
TIGRFAMsiTIGR00713 hemL, 1 hit
PROSITEiView protein in PROSITE
PS00600 AA_TRANSFER_CLASS_3, 1 hit

Sequencei

Sequence statusi: Complete.

P23893-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKSENLYSA ARELIPGGVN SPVRAFTGVG GTPLFIEKAD GAYLYDVDGK
60 70 80 90 100
AYIDYVGSWG PMVLGHNHPA IRNAVIEAAE RGLSFGAPTE MEVKMAQLVT
110 120 130 140 150
ELVPTMDMVR MVNSGTEATM SAIRLARGFT GRDKIIKFEG CYHGHADCLL
160 170 180 190 200
VKAGSGALTL GQPNSPGVPA DFAKYTLTCT YNDLASVRAA FEQYPQEIAC
210 220 230 240 250
IIVEPVAGNM NCVPPLPEFL PGLRALCDEF GALLIIDEVM TGFRVALAGA
260 270 280 290 300
QDYYGVVPDL TCLGKIIGGG MPVGAFGGRR DVMDALAPTG PVYQAGTLSG
310 320 330 340 350
NPIAMAAGFA CLNEVAQPGV HETLDELTTR LAEGLLEAAE EAGIPLVVNH
360 370 380 390 400
VGGMFGIFFT DAESVTCYQD VMACDVERFK RFFHMMLDEG VYLAPSAFEA
410 420
GFMSVAHSME DINNTIDAAR RVFAKL
Length:426
Mass (Da):45,366
Last modified:November 1, 1997 - v2
Checksum:iBED817E100468CF2
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti2S → R (PubMed:1900346).Curated1
Sequence conflicti2S → R (PubMed:8202364).Curated1
Sequence conflicti9S → Q (PubMed:1900346).Curated1
Sequence conflicti9S → Q (PubMed:8202364).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X53696 Genomic DNA Translation: CAA37734.1
U00096 Genomic DNA Translation: AAC73265.1
AP009048 Genomic DNA Translation: BAB96731.2
U70214 Genomic DNA Translation: AAB08584.1
PIRiB64739
RefSeqiNP_414696.1, NC_000913.3
WP_000045315.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC73265; AAC73265; b0154
BAB96731; BAB96731; BAB96731
GeneIDi946892
KEGGiecj:JW0150
eco:b0154
PATRICifig|1411691.4.peg.2126

Similar proteinsi

Entry informationi

Entry nameiGSA_ECOLI
AccessioniPrimary (citable) accession number: P23893
Secondary accession number(s): P78277
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: November 1, 1997
Last modified: March 28, 2018
This is version 160 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome