Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Transcriptional activator CadC

Gene

cadC

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Required for Pcad induction, a promoter upstream of cadBA that is responsible for the pH-regulated expression of CadA and CadB. Probably acts as an activating transcription factor.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi3 – 102100OmpR/PhoB-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

  • phosphorelay signal transduction system Source: UniProtKB-KW
  • positive regulation of transcription, DNA-templated Source: EcoCyc
  • transcription, DNA-templated Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation, Two-component regulatory system

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciEcoCyc:PD00436.
ECOL316407:JW4094-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcriptional activator CadC
Gene namesi
Name:cadC
Ordered Locus Names:b4133, JW4094
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10133. cadC.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei155 – 18026HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Loss of Pcad activity.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi120 – 1245PPPIP → AAAIS: Significantly reduced dependence on EF-P for translation, increased levels of protein in an efp-strain. 1 Publication
Mutagenesisi121 – 1211P → A: Reduced dependence on EF-P for translation, increased levels of protein in an efp- strain. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 512512Transcriptional activator CadCPRO_0000081345Add
BLAST

Proteomic databases

PaxDbiP23890.

Expressioni

Inductioni

Induction requires low pH plus exogenous lysine as sensed by LysP as well as EF-P for translation. This requirement is reduced as the number of consecutive Pro residues in the protein is decreased.1 Publication

Interactioni

Protein-protein interaction databases

BioGridi4260781. 6 interactions.
DIPiDIP-9239N.
IntActiP23890. 1 interaction.
STRINGi511145.b4133.

Structurei

Secondary structure

1
512
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi195 – 20410Combined sources
Beta strandi206 – 2116Combined sources
Helixi216 – 23419Combined sources
Beta strandi238 – 2414Combined sources
Beta strandi253 – 26210Combined sources
Beta strandi273 – 2808Combined sources
Turni281 – 2833Combined sources
Beta strandi286 – 2927Combined sources
Helixi299 – 31315Combined sources
Helixi320 – 32910Combined sources
Helixi334 – 3363Combined sources
Helixi337 – 34913Combined sources
Helixi352 – 36817Combined sources
Helixi373 – 38917Combined sources
Helixi394 – 40815Combined sources
Helixi411 – 4133Combined sources
Helixi417 – 43014Combined sources
Helixi433 – 44614Combined sources
Helixi450 – 46213Combined sources
Helixi466 – 47914Combined sources
Helixi483 – 4919Combined sources
Beta strandi492 – 4943Combined sources
Helixi498 – 5014Combined sources
Helixi503 – 5108Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3LY7X-ray1.80A188-512[»]
3LY8X-ray1.90A188-512[»]
3LY9X-ray2.20A188-512[»]
3LYAX-ray2.30A188-512[»]
ProteinModelPortaliP23890.
SMRiP23890. Positions 20-100, 190-511.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP23890.

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi120 – 1245Poly-Pro

Sequence similaritiesi

Contains 1 OmpR/PhoB-type DNA-binding domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4108M3R. Bacteria.
COG3710. LUCA.
HOGENOMiHOG000127085.
KOiK03765.
OMAiVPYLDNF.

Family and domain databases

CDDicd00383. trans_reg_C. 1 hit.
Gene3Di1.10.10.10. 1 hit.
InterProiIPR001867. OmpR/PhoB-type_DNA-bd.
IPR016032. Sig_transdc_resp-reg_C-effctor.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00486. Trans_reg_C. 1 hit.
[Graphical view]
SMARTiSM00862. Trans_reg_C. 1 hit.
[Graphical view]
SUPFAMiSSF46894. SSF46894. 1 hit.
PROSITEiPS51755. OMPR_PHOB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P23890-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQQPVVRVGE WLVTPSINQI SRNGRQLTLE PRLIDLLVFF AQHSGEVLSR
60 70 80 90 100
DELIDNVWKR SIVTNHVVTQ SISELRKSLK DNDEDSPVYI ATVPKRGYKL
110 120 130 140 150
MVPVIWYSEE EGEEIMLSSP PPIPEAVPAT DSPSHSLNIQ NTATPPEQSP
160 170 180 190 200
VKSKRFTTFW VWFFFLLSLG ICVALVAFSS LDTRLPMSKS RILLNPRDID
210 220 230 240 250
INMVNKSCNS WSSPYQLSYA IGVGDLVATS LNTFSTFMVH DKINYNIDEP
260 270 280 290 300
SSSGKTLSIA FVNQRQYRAQ QCFMSIKLVD NADGSTMLDK RYVITNGNQL
310 320 330 340 350
AIQNDLLESL SKALNQPWPQ RMQETLQKIL PHRGALLTNF YQAHDYLLHG
360 370 380 390 400
DDKSLNRASE LLGEIVQSSP EFTYARAEKA LVDIVRHSQH PLDEKQLAAL
410 420 430 440 450
NTEIDNIVTL PELNNLSIIY QIKAVSALVK GKTDESYQAI NTGIDLEMSW
460 470 480 490 500
LNYVLLGKVY EMKGMNREAA DAYLTAFNLR PGANTLYWIE NGIFQTSVPY
510
VVPYLDKFLA SE
Length:512
Mass (Da):57,813
Last modified:November 1, 1991 - v1
Checksum:iAA19F5E1D293ACCF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M67452 Genomic DNA. Translation: AAA23531.1.
U14003 Genomic DNA. Translation: AAA97033.1.
U00096 Genomic DNA. Translation: AAC77094.1.
AP009048 Genomic DNA. Translation: BAE78136.1.
PIRiC41968.
RefSeqiNP_418557.1. NC_000913.3.
WP_001187173.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC77094; AAC77094; b4133.
BAE78136; BAE78136; BAE78136.
GeneIDi948653.
KEGGiecj:JW4094.
eco:b4133.
PATRICi32123833. VBIEscCol129921_4265.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M67452 Genomic DNA. Translation: AAA23531.1.
U14003 Genomic DNA. Translation: AAA97033.1.
U00096 Genomic DNA. Translation: AAC77094.1.
AP009048 Genomic DNA. Translation: BAE78136.1.
PIRiC41968.
RefSeqiNP_418557.1. NC_000913.3.
WP_001187173.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3LY7X-ray1.80A188-512[»]
3LY8X-ray1.90A188-512[»]
3LY9X-ray2.20A188-512[»]
3LYAX-ray2.30A188-512[»]
ProteinModelPortaliP23890.
SMRiP23890. Positions 20-100, 190-511.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260781. 6 interactions.
DIPiDIP-9239N.
IntActiP23890. 1 interaction.
STRINGi511145.b4133.

Proteomic databases

PaxDbiP23890.

Protocols and materials databases

DNASUi948653.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC77094; AAC77094; b4133.
BAE78136; BAE78136; BAE78136.
GeneIDi948653.
KEGGiecj:JW4094.
eco:b4133.
PATRICi32123833. VBIEscCol129921_4265.

Organism-specific databases

EchoBASEiEB0131.
EcoGeneiEG10133. cadC.

Phylogenomic databases

eggNOGiENOG4108M3R. Bacteria.
COG3710. LUCA.
HOGENOMiHOG000127085.
KOiK03765.
OMAiVPYLDNF.

Enzyme and pathway databases

BioCyciEcoCyc:PD00436.
ECOL316407:JW4094-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP23890.
PROiP23890.

Family and domain databases

CDDicd00383. trans_reg_C. 1 hit.
Gene3Di1.10.10.10. 1 hit.
InterProiIPR001867. OmpR/PhoB-type_DNA-bd.
IPR016032. Sig_transdc_resp-reg_C-effctor.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00486. Trans_reg_C. 1 hit.
[Graphical view]
SMARTiSM00862. Trans_reg_C. 1 hit.
[Graphical view]
SUPFAMiSSF46894. SSF46894. 1 hit.
PROSITEiPS51755. OMPR_PHOB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCADC_ECOLI
AccessioniPrimary (citable) accession number: P23890
Secondary accession number(s): Q2M6H0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: November 1, 1991
Last modified: September 7, 2016
This is version 126 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.