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Protein

Transcription elongation factor A protein 3

Gene

Tcea3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Necessary for efficient RNA polymerase II transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by S-II allows the resumption of elongation from the new 3'-terminus.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri305 – 345TFIIS-typePROSITE-ProRule annotationAdd BLAST41

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription elongation factor A protein 3
Alternative name(s):
Transcription elongation factor S-II protein 3
Transcription elongation factor TFIIS.h
Gene namesi
Name:Tcea3
Synonyms:Tfiish
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:1196908. Tcea3.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001214531 – 347Transcription elongation factor A protein 3Add BLAST347

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei113PhosphoserineCombined sources1
Modified residuei139PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP23881.
PaxDbiP23881.
PeptideAtlasiP23881.
PRIDEiP23881.

PTM databases

iPTMnetiP23881.
PhosphoSitePlusiP23881.

Expressioni

Tissue specificityi

Liver, kidney and heart.

Gene expression databases

BgeeiENSMUSG00000001604.
CleanExiMM_TCEA3.
ExpressionAtlasiP23881. baseline and differential.
GenevisibleiP23881. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000099592.

Structurei

Secondary structure

1347
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi4 – 19Combined sources16
Helixi26 – 34Combined sources9
Helixi40 – 45Combined sources6
Helixi48 – 58Combined sources11
Helixi63 – 78Combined sources16

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1WJTNMR-A1-90[»]
ProteinModelPortaliP23881.
SMRiP23881.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP23881.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini5 – 82TFIIS N-terminalPROSITE-ProRule annotationAdd BLAST78
Domaini186 – 302TFIIS centralPROSITE-ProRule annotationAdd BLAST117

Sequence similaritiesi

Belongs to the TFS-II family.Curated
Contains 1 TFIIS central domain.PROSITE-ProRule annotation
Contains 1 TFIIS N-terminal domain.PROSITE-ProRule annotation
Contains 1 TFIIS-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri305 – 345TFIIS-typePROSITE-ProRule annotationAdd BLAST41

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG1105. Eukaryota.
COG1594. LUCA.
GeneTreeiENSGT00390000017794.
HOGENOMiHOG000195015.
HOVERGENiHBG055022.
InParanoidiP23881.
OMAiFAPSMCL.
OrthoDBiEOG091G10EJ.
PhylomeDBiP23881.
TreeFamiTF314970.

Family and domain databases

Gene3Di1.10.472.30. 1 hit.
1.20.930.10. 1 hit.
InterProiIPR016492. TF_IIS-rel.
IPR003617. TFIIS/CRSP70_N_sub.
IPR003618. TFIIS_cen_dom.
IPR017923. TFIIS_N.
IPR006289. TFSII.
IPR001222. Znf_TFIIS.
[Graphical view]
PfamiPF08711. Med26. 1 hit.
PF01096. TFIIS_C. 1 hit.
PF07500. TFIIS_M. 1 hit.
[Graphical view]
PIRSFiPIRSF006704. TF_IIS. 1 hit.
SMARTiSM00510. TFS2M. 1 hit.
SM00509. TFS2N. 1 hit.
SM00440. ZnF_C2C2. 1 hit.
[Graphical view]
SUPFAMiSSF46942. SSF46942. 1 hit.
SSF47676. SSF47676. 1 hit.
TIGRFAMsiTIGR01385. TFSII. 1 hit.
PROSITEiPS51321. TFIIS_CENTRAL. 1 hit.
PS51319. TFIIS_N. 1 hit.
PS00466. ZF_TFIIS_1. 1 hit.
PS51133. ZF_TFIIS_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P23881-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGLEEELLRI AKKLEKMVSR KKTEGALDLL KKLNSCQMSI QLLQTTRIGV
60 70 80 90 100
AVNGVRKHCS DKEVVSLAKV LIKNWKRLLD SPRTTKGERE EREKAKKEKG
110 120 130 140 150
LGCSDWKPEA GLSPPRKKGG GEPKTRRDSV DSRSSTTSSP KRPSLERSNS
160 170 180 190 200
SKSKVETPTT PSSPSTPTFA PAVCLLAPCY LTGDSVRDKC VEMLSAALKA
210 220 230 240 250
EDNFKDYGVN CDKLASEIED HIYQELKSTD MKYRNRVRSR ISNLKDPRNP
260 270 280 290 300
GLRRNVLSGA ISPELIAKMT AEEMASDELR ELRNAMTQEA IREHQMAKTG
310 320 330 340
GTTTDLLRCS KCKKKNCTYN QVQTRSADEP MTTFVLCNEC GNRWKFC
Length:347
Mass (Da):38,850
Last modified:February 12, 2003 - v3
Checksum:iA1711D4C5DAF8CC8
GO

Sequence cautioni

The sequence BAA28177 differs from that shown. Chimeric cDNA. The sequence from position 1 to 95 is due to a chimeric cDNA.Curated
The sequence BAB31514 differs from that shown. Reason: Erroneous translation. Wrong choice of frame.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti7L → P in BAB25037 (PubMed:16141072).Curated1
Sequence conflicti63 – 64EV → VL in BAB25037 (PubMed:16141072).Curated2
Sequence conflicti96 – 99KKEK → RREE in BAB25037 (PubMed:16141072).Curated4

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D00926 mRNA. Translation: BAA28177.1. Sequence problems.
AJ223472 mRNA. Translation: CAA11392.1.
AK002319 mRNA. Translation: BAB22010.1.
AK007437 mRNA. Translation: BAB25037.1.
AK019024 mRNA. Translation: BAB31514.2. Sequence problems.
AL935264 Genomic DNA. Translation: CAM13835.1.
BC010807 mRNA. Translation: AAH10807.1.
CCDSiCCDS18803.1.
RefSeqiNP_035672.1. NM_011542.2.
UniGeneiMm.112.

Genome annotation databases

EnsembliENSMUST00000102533; ENSMUSP00000099592; ENSMUSG00000001604.
GeneIDi21401.
KEGGimmu:21401.
UCSCiuc008vhv.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D00926 mRNA. Translation: BAA28177.1. Sequence problems.
AJ223472 mRNA. Translation: CAA11392.1.
AK002319 mRNA. Translation: BAB22010.1.
AK007437 mRNA. Translation: BAB25037.1.
AK019024 mRNA. Translation: BAB31514.2. Sequence problems.
AL935264 Genomic DNA. Translation: CAM13835.1.
BC010807 mRNA. Translation: AAH10807.1.
CCDSiCCDS18803.1.
RefSeqiNP_035672.1. NM_011542.2.
UniGeneiMm.112.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1WJTNMR-A1-90[»]
ProteinModelPortaliP23881.
SMRiP23881.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000099592.

PTM databases

iPTMnetiP23881.
PhosphoSitePlusiP23881.

Proteomic databases

MaxQBiP23881.
PaxDbiP23881.
PeptideAtlasiP23881.
PRIDEiP23881.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000102533; ENSMUSP00000099592; ENSMUSG00000001604.
GeneIDi21401.
KEGGimmu:21401.
UCSCiuc008vhv.2. mouse.

Organism-specific databases

CTDi6920.
MGIiMGI:1196908. Tcea3.

Phylogenomic databases

eggNOGiKOG1105. Eukaryota.
COG1594. LUCA.
GeneTreeiENSGT00390000017794.
HOGENOMiHOG000195015.
HOVERGENiHBG055022.
InParanoidiP23881.
OMAiFAPSMCL.
OrthoDBiEOG091G10EJ.
PhylomeDBiP23881.
TreeFamiTF314970.

Miscellaneous databases

ChiTaRSiTcea3. mouse.
EvolutionaryTraceiP23881.
PROiP23881.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000001604.
CleanExiMM_TCEA3.
ExpressionAtlasiP23881. baseline and differential.
GenevisibleiP23881. MM.

Family and domain databases

Gene3Di1.10.472.30. 1 hit.
1.20.930.10. 1 hit.
InterProiIPR016492. TF_IIS-rel.
IPR003617. TFIIS/CRSP70_N_sub.
IPR003618. TFIIS_cen_dom.
IPR017923. TFIIS_N.
IPR006289. TFSII.
IPR001222. Znf_TFIIS.
[Graphical view]
PfamiPF08711. Med26. 1 hit.
PF01096. TFIIS_C. 1 hit.
PF07500. TFIIS_M. 1 hit.
[Graphical view]
PIRSFiPIRSF006704. TF_IIS. 1 hit.
SMARTiSM00510. TFS2M. 1 hit.
SM00509. TFS2N. 1 hit.
SM00440. ZnF_C2C2. 1 hit.
[Graphical view]
SUPFAMiSSF46942. SSF46942. 1 hit.
SSF47676. SSF47676. 1 hit.
TIGRFAMsiTIGR01385. TFSII. 1 hit.
PROSITEiPS51321. TFIIS_CENTRAL. 1 hit.
PS51319. TFIIS_N. 1 hit.
PS00466. ZF_TFIIS_1. 1 hit.
PS51133. ZF_TFIIS_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTCEA3_MOUSE
AccessioniPrimary (citable) accession number: P23881
Secondary accession number(s): A2AW46
, O88710, Q9CTZ8, Q9DCZ5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: February 12, 2003
Last modified: November 2, 2016
This is version 143 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.