P23873 (HIPB_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Antitoxin HipB

Gene names

Name:	hipB
Ordered Locus Names:	b1508, JW1501

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	88 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Antitoxin component of a toxin-antitoxin (TA) module. Neutralizes the toxic effect of cognate toxin HipA. Binds to operator sites with the consensus sequence 5-'TATCCN8GGATA-3' to repress the hipBA operon promoter. Ref.5
Subunit structure	Homodimer. Forms a HipA2HipB2 heterotetramer which can interact with DNA. This complex also blocks the toxic activity of HipA. Ref.5
Disruption phenotype	Cannot be disrupted, suggesting it is a functional antitoxin. No visible phenotype when the hipBA operon is deleted. Ref.5
Sequence similarities	Contains 1 HTH cro/C1-type DNA-binding domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Ligand	DNA-binding
Molecular function	Repressor
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from direct assay Ref.5. Source: EcoCyc
Molecular_function	sequence-specific DNA binding Inferred from direct assay Ref.5. Source: EcoCyc
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 88

88

Antitoxin HipB

PRO_0000149726

Regions

Domain

17 – 71

55

HTH cro/C1-type

DNA binding

28 – 47

20

H-T-H motif Potential

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

88

Helix Strand Turn

Details...

Helix

10 – 23

14

Helix

28 – 35

8

Helix

39 – 47

9

Helix

49 – 51

3

Helix

54 – 63

10

Beta strand

67 – 71

5

Sequences

	Sequence		Length	Mass (Da)
	P23873 [UniParc]. Last modified November 1, 1991. Version 1. Checksum: 63C0E13C1C06CBDA	FASTA	88	10,016
	10 20 30 40 50 60 MMSFQKIYSP TQLANAMKLV RQQNGWTQSE LAKKIGIKQA TISNFENNPD NTTLTTFFKI 70 80 LQSLELSMTL CDAKNASPES TEQQNLEW

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structure and organization of hip, an operon that affects lethality due to inhibition of peptidoglycan or DNA synthesis." Black D.S., Kelly A.J., Mardis M.J., Moyed H.S. J. Bacteriol. 173:5732-5739(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map." Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Itoh T., Kasai H., Kashimoto K., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K. , Nakade S., Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Takeuchi Y., Wada C., Yamamoto Y., Horiuchi T. DNA Res. 3:363-377(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Autoregulation of hip, an operon that affects lethality due to inhibition of peptidoglycan or DNA synthesis." Black D.S., Irwin B., Moyed H.S. J. Bacteriol. 176:4081-4091(1994) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-6, CHARACTERIZATION, DNA-BINDING, FUNCTION AS A TRANSCRIPTIONAL REPRESSOR, SUBUNIT, DISRUPTION PHENOTYPE. Strain: K12.
[6]	"New kinase regulation mechanism found in HipBA: a bacterial persistence switch." Evdokimov A., Voznesensky I., Fennell K., Anderson M., Smith J.F., Fisher D.A. Acta Crystallogr. D 65:875-879(2009) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.35 ANGSTROMS) IN COMPLEX WITH HIPA. Strain: K12 / DH5-alpha.
[7]	"Molecular mechanisms of HipA-mediated multidrug tolerance and its neutralization by HipB." Schumacher M.A., Piro K.M., Xu W., Hansen S., Lewis K., Brennan R.G. Science 323:396-401(2009) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.68 ANGSTROMS) IN COMPLEX WITH HIPA AND DNA.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M61242 Genomic DNA. Translation: AAA56877.1.
U00096 Genomic DNA. Translation: AAC74581.1.
AP009048 Genomic DNA. Translation: BAA15180.1.

PIR

A38112.

RefSeq

NP_416025.1. NC_000913.2.
YP_489772.1. NC_007779.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2WIU	X-ray	2.35	B/D	1-88	[»]
3DNV	X-ray	2.68	B	1-88	[»]
3HZI	X-ray	2.98	B	1-88	[»]

ProteinModelPortal

P23873.

SMR

P23873. Positions 4-74.

ModBase

Search...

Protein-protein interaction databases

IntAct

P23873. 1 interaction.

STRING

511145.b1508.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

AAC74581; AAC74581; b1508.
BAA15180; BAA15180; BAA15180.

GeneID

12931227.
946065.

KEGG

ecj:Y75_p1483.
eco:b1508.

PATRIC

32118310. VBIEscCol129921_1575.

Organism-specific databases

EchoBASE

EB0437.

EcoGene

EG10442. hipB.

Phylogenomic databases

eggNOG

COG1396.

HOGENOM

HOG000225389.

KO

K15773.

OMA

HALEVHC.

ProtClustDB

PRK09726.

Enzyme and pathway databases

BioCyc

EcoCyc:EG10442-MONOMER.
ECOL316407:JW1501-MONOMER.

Gene expression databases

Genevestigator

P23873.

Family and domain databases

Gene3D

1.10.260.40. 1 hit.

InterPro

IPR001387. HTH.
IPR010982. Lambda_DNA-bd_dom.
[Graphical view]

Pfam

PF01381. HTH_3. 1 hit.
[Graphical view]

SMART

SM00530. HTH_XRE. 1 hit.
[Graphical view]

SUPFAM

SSF47413. Lambda_like_DNA. 1 hit.

PROSITE

PS50943. HTH_CROC1. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P23873.

Entry information

Entry name

HIPB_ECOLI

Accession

Primary (citable) accession number: P23873

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1991
Last sequence update:	November 1, 1991
Last modified:	May 1, 2013
This is version 97 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program