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Protein

Ferrochelatase

Gene

hemH

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the ferrous insertion into protoporphyrin IX.

Catalytic activityi

Protoheme + 2 H+ = protoporphyrin + Fe2+.

Pathwayi: protoheme biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes protoheme from protoporphyrin-IX.
Proteins known to be involved in this subpathway in this organism are:
  1. Ferrochelatase (hemH)
This subpathway is part of the pathway protoheme biosynthesis, which is itself part of Porphyrin-containing compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes protoheme from protoporphyrin-IX, the pathway protoheme biosynthesis and in Porphyrin-containing compound metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi194IronBy similarity1
Metal bindingi275IronBy similarity1

GO - Molecular functioni

  • ferrochelatase activity Source: EcoCyc
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • heme biosynthetic process Source: EcoliWiki
  • protoporphyrinogen IX metabolic process Source: EcoliWiki
  • response to light stimulus Source: EcoliWiki

Keywordsi

Molecular functionLyase
Biological processHeme biosynthesis, Porphyrin biosynthesis
LigandIron, Metal-binding

Enzyme and pathway databases

BioCyciEcoCyc:PROTOHEME-FERROCHELAT-MONOMER
MetaCyc:PROTOHEME-FERROCHELAT-MONOMER
UniPathwayiUPA00252; UER00325

Names & Taxonomyi

Protein namesi
Recommended name:
Ferrochelatase (EC:4.99.1.1)
Alternative name(s):
Heme synthase
Protoheme ferro-lyase
Gene namesi
Name:hemH
Synonyms:popA, visA
Ordered Locus Names:b0475, JW0464
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10431 hemH

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Cells are sensitive to visible light (which is lethal).1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001751381 – 320FerrochelataseAdd BLAST320

Proteomic databases

PaxDbiP23871
PRIDEiP23871

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

BioGridi4259849, 7 interactors
IntActiP23871, 9 interactors
STRINGi316385.ECDH10B_0431

Structurei

3D structure databases

ProteinModelPortaliP23871
SMRiP23871
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ferrochelatase family.Curated

Phylogenomic databases

eggNOGiENOG4105CFX Bacteria
COG0276 LUCA
HOGENOMiHOG000060730
InParanoidiP23871
KOiK01772
OMAiLGDPYHC
PhylomeDBiP23871

Family and domain databases

CDDicd00419 Ferrochelatase_C, 1 hit
cd03411 Ferrochelatase_N, 1 hit
HAMAPiMF_00323 Ferrochelatase, 1 hit
InterProiView protein in InterPro
IPR001015 Ferrochelatase
IPR019772 Ferrochelatase_AS
IPR033644 Ferrochelatase_C
IPR033659 Ferrochelatase_N
PANTHERiPTHR11108 PTHR11108, 1 hit
PfamiView protein in Pfam
PF00762 Ferrochelatase, 1 hit
TIGRFAMsiTIGR00109 hemH, 1 hit
PROSITEiView protein in PROSITE
PS00534 FERROCHELATASE, 1 hit

Sequencei

Sequence statusi: Complete.

P23871-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRQTKTGILL ANLGTPDAPT PEAVKRYLKQ FLSDRRVVDT SRLLWWPLLR
60 70 80 90 100
GVILPLRSPR VAKLYASVWM EGGSPLMVYS RQQQQALAQR LPEMPVALGM
110 120 130 140 150
SYGSPSLESA VDELLAEHVD HIVVLPLYPQ FSCSTVGAVW DELARILARK
160 170 180 190 200
RSIPGISFIR DYADNHDYIN ALANSVRASF AKHGEPDLLL LSYHGIPQRY
210 220 230 240 250
ADEGDDYPQR CRTTTRELAS ALGMAPEKVM MTFQSRFGRE PWLMPYTDET
260 270 280 290 300
LKMLGEKGVG HIQVMCPGFA ADCLETLEEI AEQNREVFLG AGGKKYEYIP
310 320
ALNATPEHIE MMANLVAAYR
Length:320
Mass (Da):35,884
Last modified:November 1, 1997 - v3
Checksum:i65BB56EBFDD95D5C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti54L → F in BAA14304 (PubMed:2051480).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D90259 Genomic DNA Translation: BAA14304.1
U82664 Genomic DNA Translation: AAB40229.1
U00096 Genomic DNA Translation: AAC73577.1
AP009048 Genomic DNA Translation: BAE76254.1
PIRiB64778
RefSeqiNP_415008.1, NC_000913.3
WP_001250103.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC73577; AAC73577; b0475
BAE76254; BAE76254; BAE76254
GeneIDi947532
KEGGiecj:JW0464
eco:b0475
PATRICifig|1411691.4.peg.1801

Similar proteinsi

Entry informationi

Entry nameiHEMH_ECOLI
AccessioniPrimary (citable) accession number: P23871
Secondary accession number(s): P78232, Q2MBV2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: November 1, 1997
Last modified: March 28, 2018
This is version 140 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

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