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Protein

Tail-specific protease

Gene

prc

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in the cleavage of a C-terminal peptide of 11 residues from the precursor form of penicillin-binding protein 3 (PBP3). May be involved in protection of the bacterium from thermal and osmotic stresses.

Catalytic activityi

The enzyme shows specific recognition of a C-terminal tripeptide, Xaa-Yaa-Zaa, in which Xaa is preferably Ala or Leu, Yaa is preferably Ala or Tyr, and Zaa is preferably Ala, but then cleaves at a variable distance from the C-terminus. A typical cleavage is -Ala-Ala-|-Arg-Ala-Ala-Lys-Glu-Asn-Tyr-Ala-Leu-Ala-Ala.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei452Charge relay system1
Active sitei463Charge relay systemCurated1
Active sitei477Charge relay system1

GO - Molecular functioni

  • endopeptidase activity Source: EcoliWiki
  • serine-type peptidase activity Source: UniProtKB-KW

GO - Biological processi

  • protein catabolic process Source: EcoliWiki
  • proteolysis Source: EcoliWiki
  • response to antibiotic Source: EcoCyc
  • signal transduction Source: GO_Central

Keywordsi

Molecular functionHydrolase, Protease, Serine protease

Enzyme and pathway databases

BioCyciEcoCyc:EG10760-MONOMER
MetaCyc:EG10760-MONOMER
BRENDAi3.4.21.102 2026

Protein family/group databases

MEROPSiS41.001
TCDBi9.B.174.1.2 the two tunnel gated c-terminal processing protease (ctp) family

Names & Taxonomyi

Protein namesi
Recommended name:
Tail-specific protease (EC:3.4.21.102)
Alternative name(s):
C-terminal-processing peptidase
PRC protein
Protease Re
Gene namesi
Name:prc
Synonyms:tsp
Ordered Locus Names:b1830, JW1819
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10760 prc

Subcellular locationi

GO - Cellular componenti

  • outer membrane-bounded periplasmic space Source: EcoCyc
  • plasma membrane Source: UniProtKB-SubCell

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi397G → A: Loss of activity. Perturbs protein structure. 1 Publication1
Mutagenesisi398G → A: Loss of activity. Perturbs protein structure. 1 Publication1
Mutagenesisi452S → A: Loss of activity. 1 Publication1
Mutagenesisi452S → C: Reduces activity by over 90%. 1 Publication1
Mutagenesisi455E → A: Loss of activity. Perturbs protein structure. 1 Publication1
Mutagenesisi463D → A: Loss of activity. 1 Publication1
Mutagenesisi463D → N: Reduces activity by 90%. 1 Publication1
Mutagenesisi474T → A: Loss of activity. Perturbs protein structure. 1 Publication1
Mutagenesisi477K → A or H: Loss of activity. No apparent effect on protein structure. 1 Publication1
Mutagenesisi477K → R: Loss of activity. Perturbs protein structure. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 22Sequence analysisAdd BLAST22
ChainiPRO_000002733123 – 682Tail-specific proteaseAdd BLAST660

Proteomic databases

EPDiP23865
PaxDbiP23865
PRIDEiP23865

Interactioni

Protein-protein interaction databases

BioGridi4263463512 interactors.
DIPiDIP-10557N
IntActiP23865 3 interactors.
STRINGi316385.ECDH10B_1969

Structurei

Secondary structure

1682
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi29 – 31Combined sources3
Helixi41 – 54Combined sources14
Helixi64 – 78Combined sources15
Helixi87 – 94Combined sources8
Helixi95 – 99Combined sources5
Helixi100 – 106Combined sources7
Helixi110 – 134Combined sources25
Beta strandi145 – 148Combined sources4
Helixi159 – 178Combined sources20
Turni179 – 181Combined sources3
Helixi184 – 203Combined sources20
Helixi207 – 222Combined sources16
Beta strandi227 – 229Combined sources3
Helixi231 – 242Combined sources12
Beta strandi243 – 247Combined sources5
Beta strandi249 – 257Combined sources9
Beta strandi259 – 264Combined sources6
Turni269 – 272Combined sources4
Beta strandi281 – 290Combined sources10
Helixi300 – 307Combined sources8
Beta strandi314 – 320Combined sources7
Beta strandi322 – 325Combined sources4
Beta strandi329 – 334Combined sources6
Beta strandi336 – 339Combined sources4
Helixi341 – 343Combined sources3
Beta strandi346 – 352Combined sources7
Beta strandi355 – 361Combined sources7
Helixi369 – 382Combined sources14
Beta strandi386 – 391Combined sources6
Beta strandi397 – 399Combined sources3
Helixi400 – 408Combined sources9
Beta strandi413 – 420Combined sources8
Beta strandi426 – 430Combined sources5
Beta strandi442 – 446Combined sources5
Helixi453 – 463Combined sources11
Beta strandi467 – 472Combined sources6
Beta strandi479 – 485Combined sources7
Helixi491 – 494Combined sources4
Beta strandi503 – 512Combined sources10
Turni520 – 522Combined sources3
Beta strandi527 – 529Combined sources3
Helixi531 – 534Combined sources4
Helixi541 – 543Combined sources3
Helixi565 – 567Combined sources3
Helixi568 – 580Combined sources13
Helixi583 – 598Combined sources16
Helixi599 – 601Combined sources3
Beta strandi604 – 606Combined sources3
Helixi609 – 633Combined sources25
Helixi642 – 644Combined sources3
Helixi654 – 670Combined sources17

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5WQLX-ray2.30C/D1-682[»]
ProteinModelPortaliP23865
SMRiP23865
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini238 – 322PDZPROSITE-ProRule annotationAdd BLAST85

Sequence similaritiesi

Belongs to the peptidase S41A family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4105CN1 Bacteria
COG0793 LUCA
HOGENOMiHOG000277886
InParanoidiP23865
KOiK03797
OMAiIEIPAFY
PhylomeDBiP23865

Family and domain databases

CDDicd07560 Peptidase_S41_CPP, 1 hit
InterProiView protein in InterPro
IPR029045 ClpP/crotonase-like_dom_sf
IPR001478 PDZ
IPR036034 PDZ_sf
IPR004447 Peptidase_S41A
IPR005151 Tail-specific_protease
IPR020992 Tail_Prtase_C
PfamiView protein in Pfam
PF11818 DUF3340, 1 hit
PF00595 PDZ, 1 hit
PF03572 Peptidase_S41, 1 hit
SMARTiView protein in SMART
SM00228 PDZ, 1 hit
SM00245 TSPc, 1 hit
SUPFAMiSSF50156 SSF50156, 1 hit
SSF52096 SSF52096, 2 hits
TIGRFAMsiTIGR00225 prc, 1 hit
PROSITEiView protein in PROSITE
PS50106 PDZ, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P23865-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNMFFRLTAL AGLLAIAGQT FAVEDITRAD QIPVLKEETQ HATVSERVTS
60 70 80 90 100
RFTRSHYRQF DLDQAFSAKI FDRYLNLLDY SHNVLLASDV EQFAKKKTEL
110 120 130 140 150
GDELRSGKLD VFYDLYNLAQ KRRFERYQYA LSVLEKPMDF TGNDTYNLDR
160 170 180 190 200
SKAPWPKNEA ELNALWDSKV KFDELSLKLT GKTDKEIRET LTRRYKFAIR
210 220 230 240 250
RLAQTNSEDV FSLAMTAFAR EIDPHTNYLS PRNTEQFNTE MSLSLEGIGA
260 270 280 290 300
VLQMDDDYTV INSMVAGGPA AKSKAISVGD KIVGVGQTGK PMVDVIGWRL
310 320 330 340 350
DDVVALIKGP KGSKVRLEIL PAGKGTKTRT VTLTRERIRL EDRAVKMSVK
360 370 380 390 400
TVGKEKVGVL DIPGFYVGLT DDVKVQLQKL EKQNVSSVII DLRSNGGGAL
410 420 430 440 450
TEAVSLSGLF IPAGPIVQVR DNNGKVREDS DTDGQVFYKG PLVVLVDRFS
460 470 480 490 500
ASASEIFAAA MQDYGRALVV GEPTFGKGTV QQYRSLNRIY DQMLRPEWPA
510 520 530 540 550
LGSVQYTIQK FYRVNGGSTQ RKGVTPDIIM PTGNEETETG EKFEDNALPW
560 570 580 590 600
DSIDAATYVK SGDLTAFEPE LLKEHNARIA KDPEFQNIMK DIARFNAMKD
610 620 630 640 650
KRNIVSLNYA VREKENNEDD ATRLARLNER FKREGKPELK KLDDLPKDYQ
660 670 680
EPDPYLDETV NIALDLAKLE KARPAEQPAP VK
Length:682
Mass (Da):76,663
Last modified:November 1, 1997 - v2
Checksum:i49D1DE9574CCD4BB
GO

Sequence cautioni

The sequence BAA00578 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti317L → Q in BAA00577 (PubMed:1856173).Curated1
Sequence conflicti317L → Q in BAA00578 (PubMed:1856173).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D00674 Genomic DNA Translation: BAA00577.1
D00674 Genomic DNA Translation: BAA00578.1 Different initiation.
M75634 Genomic DNA Translation: AAA24699.1
U00096 Genomic DNA Translation: AAC74900.1
AP009048 Genomic DNA Translation: BAA15638.1
S49803 Genomic DNA Translation: AAB24313.1
L48409 Genomic DNA Translation: AAD41528.1
PIRiA41798
RefSeqiNP_416344.1, NC_000913.3
WP_001055791.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC74900; AAC74900; b1830
BAA15638; BAA15638; BAA15638
GeneIDi946096
KEGGiecj:JW1819
eco:b1830
PATRICifig|511145.12.peg.1908

Similar proteinsi

Entry informationi

Entry nameiPRC_ECOLI
AccessioniPrimary (citable) accession number: P23865
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: November 1, 1997
Last modified: March 28, 2018
This is version 159 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome