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Protein

Tail-specific protease

Gene

prc

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the cleavage of a C-terminal peptide of 11 residues from the precursor form of penicillin-binding protein 3 (PBP3). May be involved in protection of the bacterium from thermal and osmotic stresses.

Catalytic activityi

The enzyme shows specific recognition of a C-terminal tripeptide, Xaa-Yaa-Zaa, in which Xaa is preferably Ala or Leu, Yaa is preferably Ala or Tyr, and Zaa is preferably Ala, but then cleaves at a variable distance from the C-terminus. A typical cleavage is -Ala-Ala-|-Arg-Ala-Ala-Lys-Glu-Asn-Tyr-Ala-Leu-Ala-Ala.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei452Charge relay system1
Active sitei463Charge relay systemCurated1
Active sitei477Charge relay system1

GO - Molecular functioni

  • endopeptidase activity Source: EcoliWiki
  • serine-type peptidase activity Source: UniProtKB-KW

GO - Biological processi

  • protein catabolic process Source: EcoliWiki
  • proteolysis Source: EcoliWiki
  • response to antibiotic Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Enzyme and pathway databases

BioCyciEcoCyc:EG10760-MONOMER.
ECOL316407:JW1819-MONOMER.
MetaCyc:EG10760-MONOMER.
BRENDAi3.4.21.102. 2026.

Protein family/group databases

MEROPSiS41.001.
TCDBi9.B.174.1.2. the two tunnel gated c-terminal processing protease (ctp) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Tail-specific protease (EC:3.4.21.102)
Alternative name(s):
C-terminal-processing peptidase
PRC protein
Protease Re
Gene namesi
Name:prc
Synonyms:tsp
Ordered Locus Names:b1830, JW1819
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10760. prc.

Subcellular locationi

GO - Cellular componenti

  • outer membrane-bounded periplasmic space Source: EcoCyc
  • plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi397G → A: Loss of activity. Perturbs protein structure. 1 Publication1
Mutagenesisi398G → A: Loss of activity. Perturbs protein structure. 1 Publication1
Mutagenesisi452S → A: Loss of activity. 1 Publication1
Mutagenesisi452S → C: Reduces activity by over 90%. 1 Publication1
Mutagenesisi455E → A: Loss of activity. Perturbs protein structure. 1 Publication1
Mutagenesisi463D → A: Loss of activity. 1 Publication1
Mutagenesisi463D → N: Reduces activity by 90%. 1 Publication1
Mutagenesisi474T → A: Loss of activity. Perturbs protein structure. 1 Publication1
Mutagenesisi477K → A or H: Loss of activity. No apparent effect on protein structure. 1 Publication1
Mutagenesisi477K → R: Loss of activity. Perturbs protein structure. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 22Sequence analysisAdd BLAST22
ChainiPRO_000002733123 – 682Tail-specific proteaseAdd BLAST660

Proteomic databases

EPDiP23865.
PaxDbiP23865.
PRIDEiP23865.

Interactioni

Protein-protein interaction databases

BioGridi4263463. 432 interactors.
DIPiDIP-10557N.
IntActiP23865. 3 interactors.
MINTiMINT-1283244.
STRINGi511145.b1830.

Structurei

3D structure databases

ProteinModelPortaliP23865.
SMRiP23865.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini238 – 322PDZPROSITE-ProRule annotationAdd BLAST85

Sequence similaritiesi

Belongs to the peptidase S41A family.Curated
Contains 1 PDZ (DHR) domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4105CN1. Bacteria.
COG0793. LUCA.
HOGENOMiHOG000277886.
InParanoidiP23865.
KOiK03797.
OMAiIEIPAFY.
PhylomeDBiP23865.

Family and domain databases

Gene3Di2.30.42.10. 1 hit.
3.90.226.10. 2 hits.
InterProiIPR029045. ClpP/crotonase-like_dom.
IPR001478. PDZ.
IPR004447. Peptidase_S41A.
IPR005151. Tail-specific_protease.
IPR020992. Tail_Prtase_C.
[Graphical view]
PfamiPF11818. DUF3340. 1 hit.
PF00595. PDZ. 1 hit.
PF03572. Peptidase_S41. 1 hit.
[Graphical view]
SMARTiSM00228. PDZ. 1 hit.
SM00245. TSPc. 1 hit.
[Graphical view]
SUPFAMiSSF50156. SSF50156. 1 hit.
SSF52096. SSF52096. 2 hits.
TIGRFAMsiTIGR00225. prc. 1 hit.
PROSITEiPS50106. PDZ. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P23865-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNMFFRLTAL AGLLAIAGQT FAVEDITRAD QIPVLKEETQ HATVSERVTS
60 70 80 90 100
RFTRSHYRQF DLDQAFSAKI FDRYLNLLDY SHNVLLASDV EQFAKKKTEL
110 120 130 140 150
GDELRSGKLD VFYDLYNLAQ KRRFERYQYA LSVLEKPMDF TGNDTYNLDR
160 170 180 190 200
SKAPWPKNEA ELNALWDSKV KFDELSLKLT GKTDKEIRET LTRRYKFAIR
210 220 230 240 250
RLAQTNSEDV FSLAMTAFAR EIDPHTNYLS PRNTEQFNTE MSLSLEGIGA
260 270 280 290 300
VLQMDDDYTV INSMVAGGPA AKSKAISVGD KIVGVGQTGK PMVDVIGWRL
310 320 330 340 350
DDVVALIKGP KGSKVRLEIL PAGKGTKTRT VTLTRERIRL EDRAVKMSVK
360 370 380 390 400
TVGKEKVGVL DIPGFYVGLT DDVKVQLQKL EKQNVSSVII DLRSNGGGAL
410 420 430 440 450
TEAVSLSGLF IPAGPIVQVR DNNGKVREDS DTDGQVFYKG PLVVLVDRFS
460 470 480 490 500
ASASEIFAAA MQDYGRALVV GEPTFGKGTV QQYRSLNRIY DQMLRPEWPA
510 520 530 540 550
LGSVQYTIQK FYRVNGGSTQ RKGVTPDIIM PTGNEETETG EKFEDNALPW
560 570 580 590 600
DSIDAATYVK SGDLTAFEPE LLKEHNARIA KDPEFQNIMK DIARFNAMKD
610 620 630 640 650
KRNIVSLNYA VREKENNEDD ATRLARLNER FKREGKPELK KLDDLPKDYQ
660 670 680
EPDPYLDETV NIALDLAKLE KARPAEQPAP VK
Length:682
Mass (Da):76,663
Last modified:November 1, 1997 - v2
Checksum:i49D1DE9574CCD4BB
GO

Sequence cautioni

The sequence BAA00578 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti317L → Q in BAA00577 (PubMed:1856173).Curated1
Sequence conflicti317L → Q in BAA00578 (PubMed:1856173).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D00674 Genomic DNA. Translation: BAA00577.1.
D00674 Genomic DNA. Translation: BAA00578.1. Different initiation.
M75634 Genomic DNA. Translation: AAA24699.1.
U00096 Genomic DNA. Translation: AAC74900.1.
AP009048 Genomic DNA. Translation: BAA15638.1.
S49803 Genomic DNA. Translation: AAB24313.1.
L48409 Genomic DNA. Translation: AAD41528.1.
PIRiA41798.
RefSeqiNP_416344.1. NC_000913.3.
WP_001055791.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74900; AAC74900; b1830.
BAA15638; BAA15638; BAA15638.
GeneIDi946096.
KEGGiecj:JW1819.
eco:b1830.
PATRICi32118981. VBIEscCol129921_1908.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D00674 Genomic DNA. Translation: BAA00577.1.
D00674 Genomic DNA. Translation: BAA00578.1. Different initiation.
M75634 Genomic DNA. Translation: AAA24699.1.
U00096 Genomic DNA. Translation: AAC74900.1.
AP009048 Genomic DNA. Translation: BAA15638.1.
S49803 Genomic DNA. Translation: AAB24313.1.
L48409 Genomic DNA. Translation: AAD41528.1.
PIRiA41798.
RefSeqiNP_416344.1. NC_000913.3.
WP_001055791.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP23865.
SMRiP23865.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4263463. 432 interactors.
DIPiDIP-10557N.
IntActiP23865. 3 interactors.
MINTiMINT-1283244.
STRINGi511145.b1830.

Protein family/group databases

MEROPSiS41.001.
TCDBi9.B.174.1.2. the two tunnel gated c-terminal processing protease (ctp) family.

Proteomic databases

EPDiP23865.
PaxDbiP23865.
PRIDEiP23865.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74900; AAC74900; b1830.
BAA15638; BAA15638; BAA15638.
GeneIDi946096.
KEGGiecj:JW1819.
eco:b1830.
PATRICi32118981. VBIEscCol129921_1908.

Organism-specific databases

EchoBASEiEB0753.
EcoGeneiEG10760. prc.

Phylogenomic databases

eggNOGiENOG4105CN1. Bacteria.
COG0793. LUCA.
HOGENOMiHOG000277886.
InParanoidiP23865.
KOiK03797.
OMAiIEIPAFY.
PhylomeDBiP23865.

Enzyme and pathway databases

BioCyciEcoCyc:EG10760-MONOMER.
ECOL316407:JW1819-MONOMER.
MetaCyc:EG10760-MONOMER.
BRENDAi3.4.21.102. 2026.

Miscellaneous databases

PROiP23865.

Family and domain databases

Gene3Di2.30.42.10. 1 hit.
3.90.226.10. 2 hits.
InterProiIPR029045. ClpP/crotonase-like_dom.
IPR001478. PDZ.
IPR004447. Peptidase_S41A.
IPR005151. Tail-specific_protease.
IPR020992. Tail_Prtase_C.
[Graphical view]
PfamiPF11818. DUF3340. 1 hit.
PF00595. PDZ. 1 hit.
PF03572. Peptidase_S41. 1 hit.
[Graphical view]
SMARTiSM00228. PDZ. 1 hit.
SM00245. TSPc. 1 hit.
[Graphical view]
SUPFAMiSSF50156. SSF50156. 1 hit.
SSF52096. SSF52096. 2 hits.
TIGRFAMsiTIGR00225. prc. 1 hit.
PROSITEiPS50106. PDZ. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPRC_ECOLI
AccessioniPrimary (citable) accession number: P23865
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: November 1, 1997
Last modified: November 2, 2016
This is version 146 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.