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Protein

Primosomal replication protein N''

Gene

priC

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

PriA recognizes a specific hairpin sequence on bacteriophage phi X174 ssDNA. This structure is then recognized and bound by proteins PriB and PriC. Formation of the primosome proceeds with the subsequent actions of DnaB, DnaC, DnaT and primase.

GO - Molecular functioni

  • DNA binding Source: EcoCyc

GO - Biological processi

  • DNA-dependent DNA replication Source: EcoCyc
  • DNA replication, synthesis of RNA primer Source: UniProtKB-KW
  • DNA replication initiation Source: EcoCyc
Complete GO annotation...

Enzyme and pathway databases

BioCyciEcoCyc:EG10765-MONOMER.
ECOL316407:JW0456-MONOMER.
MetaCyc:EG10765-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Primosomal replication protein N''
Gene namesi
Name:priC
Ordered Locus Names:b0467, JW0456
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10765. priC.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Primosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved1 Publication
Chaini2 – 175174Primosomal replication protein N''PRO_0000058574Add
BLAST

Proteomic databases

PaxDbiP23862.
PRIDEiP23862.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
crpP0ACJ82EBI-1117383,EBI-547513
dnaBP0ACB05EBI-1117383,EBI-548978
rhmRP777323EBI-1117383,EBI-549004

Protein-protein interaction databases

BioGridi4262816. 50 interactions.
DIPiDIP-10564N.
IntActiP23862. 25 interactions.
STRINGi511145.b0467.

Structurei

Secondary structure

1
175
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi3 – 2018Combined sources
Turni23 – 275Combined sources
Turni36 – 383Combined sources
Helixi46 – 6318Combined sources
Helixi64 – 663Combined sources
Helixi69 – 9325Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2RT6NMR-A1-98[»]
ProteinModelPortaliP23862.
SMRiP23862. Positions 1-98.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Phylogenomic databases

eggNOGiENOG4108URS. Bacteria.
COG3923. LUCA.
HOGENOMiHOG000117643.
KOiK04067.
OMAiQHQEYER.
OrthoDBiEOG6XDGW7.

Family and domain databases

InterProiIPR010890. Primosomal_replicat_PriB/PriC.
[Graphical view]
PfamiPF07445. PriC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P23862-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKTALLLEKL EGQLATLRQR CAPVSQFATL SARFDRHLFQ TRATTLQACL
60 70 80 90 100
DEAGDNLAAL RHAVEQQQLP QVAWLAEHLA AQLEAIAREA SAWSLREWDS
110 120 130 140 150
APPKIARWQR KRIQHQDFER RLREMVAERR ARLARVTDLV EQQTLHREVE
160 170
AYEARLARCR HALEKIENRL ARLTR
Length:175
Mass (Da):20,375
Last modified:January 23, 2007 - v3
Checksum:i6C735A9CAABB82B3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D13958 Genomic DNA. Translation: BAA03055.1.
U82664 Genomic DNA. Translation: AAB40221.1.
U00096 Genomic DNA. Translation: AAC73569.1.
AP009048 Genomic DNA. Translation: BAE76246.1.
PIRiJQ1149.
RefSeqiNP_415000.1. NC_000913.3.
WP_000844874.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73569; AAC73569; b0467.
BAE76246; BAE76246; BAE76246.
GeneIDi945071.
KEGGiecj:JW0456.
eco:b0467.
PATRICi32116089. VBIEscCol129921_0486.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D13958 Genomic DNA. Translation: BAA03055.1.
U82664 Genomic DNA. Translation: AAB40221.1.
U00096 Genomic DNA. Translation: AAC73569.1.
AP009048 Genomic DNA. Translation: BAE76246.1.
PIRiJQ1149.
RefSeqiNP_415000.1. NC_000913.3.
WP_000844874.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2RT6NMR-A1-98[»]
ProteinModelPortaliP23862.
SMRiP23862. Positions 1-98.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262816. 50 interactions.
DIPiDIP-10564N.
IntActiP23862. 25 interactions.
STRINGi511145.b0467.

Proteomic databases

PaxDbiP23862.
PRIDEiP23862.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73569; AAC73569; b0467.
BAE76246; BAE76246; BAE76246.
GeneIDi945071.
KEGGiecj:JW0456.
eco:b0467.
PATRICi32116089. VBIEscCol129921_0486.

Organism-specific databases

EchoBASEiEB0758.
EcoGeneiEG10765. priC.

Phylogenomic databases

eggNOGiENOG4108URS. Bacteria.
COG3923. LUCA.
HOGENOMiHOG000117643.
KOiK04067.
OMAiQHQEYER.
OrthoDBiEOG6XDGW7.

Enzyme and pathway databases

BioCyciEcoCyc:EG10765-MONOMER.
ECOL316407:JW0456-MONOMER.
MetaCyc:EG10765-MONOMER.

Miscellaneous databases

PROiP23862.

Family and domain databases

InterProiIPR010890. Primosomal_replicat_PriB/PriC.
[Graphical view]
PfamiPF07445. PriC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The priB and priC replication proteins of Escherichia coli. Genes, DNA sequence, overexpression, and purification."
    Zavitz K.H., Digate R.J., Marians K.J.
    J. Biol. Chem. 266:13988-13995(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-19.
    Strain: K12.
  2. "Sequence of minutes 4-25 of Escherichia coli."
    Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.
    Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Entry informationi

Entry nameiPRIC_ECOLI
AccessioniPrimary (citable) accession number: P23862
Secondary accession number(s): Q2MBW0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: January 23, 2007
Last modified: April 13, 2016
This is version 109 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.